| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,502,577 | G→A | P637S (CCG→TCG) | fryA ← | putative PTS enzyme: Hpr, enzyme I and IIA components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,502,577 | 0 | G | A | 98.3% | 388.0 / NA | 118 | P637S (CCG→TCG) | fryA | putative PTS enzyme: Hpr, enzyme I and IIA components |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (63/52); minor base T (1/1); total (64/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 118 total aligned reads displayed.
ACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGGGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTCCACC > NC_000913/2502282‑2502872 | ccAATCTCTTGCCCTCGCCCGCCATATACTTCCAGCTCAGTCGGGTGTTCAGTGCGCCCGTTAACGCCGAGTTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACACAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCtac < 2:332692/300‑1 (MQ=255) aTCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCtactca < 1:773259/301‑1 (MQ=255) tctTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCtactcata > 1:215122/1‑300 (MQ=255) tCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGACGCTGGCTTTTCACCGCCGGAATACGCGTCCtacccataccc > 2:783947/1‑298 (MQ=255) ccGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGTCTCGCTACCCTGTTGCCGAACCTGGCTTTTCACCTCCGGTATACGGCAGCTCCTCATACCCAg > 2:220039/1‑300 (MQ=255) ccGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGc < 2:214906/301‑1 (MQ=255) cAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGCATACGCGAGCCACTCATACTCAGCTCATCCa > 1:585387/1‑301 (MQ=255) tcttctTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGACTACGCCAGCTCCCCATACTCAGCTCATCCAGgccc > 2:493246/1‑300 (MQ=255) tcccgggacAAACGGATGTTAAGCGCGTCCGTTAACGCCAAGGTTGCCGAACCGGAACTGGAACGCCTGCTCTTTAGTGGTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGGAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGGGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGgcccagccca < 2:951834/293‑1 (MQ=255) tcccAGCTCACACTGATGTTCAGTGCTCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAATGCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGgcccagccca < 2:104878/299‑1 (MQ=255) ccAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAGTACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAg > 1:556925/1‑301 (MQ=255) cAAACGGAGGTCCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAg < 2:370072/301‑1 (MQ=255) accGGACGTTCGGGGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCg < 1:347645/299‑1 (MQ=255) cGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCgg > 1:272814/1‑300 (MQ=255) aTGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGa > 1:622208/1‑301 (MQ=255) gTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCCGGCCCAGCCCAAGCAGTAGCGGCCGATa > 2:814940/1‑301 (MQ=255) tGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACg < 1:850634/298‑1 (MQ=255) tGCGCCCGTAAACGCCAAGGTTGCCGCACAGAAACTTGATTGCCTGCTCATTATTGCTAAAATCCTGGTCAACAAATATATTCGCCAGCGCCAGCATTAGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGGGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTGCCAGACACGCCTCGCTATACAGTTGGCGAAGCTTGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGc < 2:471361/300‑1 (MQ=255) cGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGCTATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTTAGTAACGCTTCAATTTCCTGCGCCCTGCGGCATTCACATGCCTGACGCG‑CCCGATCCCGCCACTCCTCGCCATCCAGTTGACGACGCTGGCTTTTCACGGCCGGAACACGCGAGCTACTCGTTCACAGCTCATCGAGCCCCGGCCCCAGCATCAGCGGCTGCTAACTGCtttgccctgccaa > 2:820341/1‑291 (MQ=255) ggTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTccc > 2:291651/1‑298 (MQ=255) gtGCCTCACAGGAACGGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGTTGTTCTCCAGCGCCAGCAGTGGGCGAACGTCTGCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAGTACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCg < 2:556314/299‑1 (MQ=255) ccGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCaaa > 1:1052202/1‑301 (MQ=255) caGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGTCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGCCGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAGTACGCGAGCTACTCATACTCAGCTCACCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGTTTTCACCGCCCAGTTCATCGCAAATGc > 1:292901/1‑299 (MQ=255) tGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCCAATGCCTACCCAtt > 1:257333/1‑301 (MQ=255) gCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTCGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGccc > 2:925892/1‑300 (MQ=255) ccTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGcccc > 1:1008834/1‑300 (MQ=255) tGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:815874/301‑1 (MQ=255) ctctTTATTGCTAAAATCCTGATACACAAAGATATCCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGTCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTg < 1:821325/300‑1 (MQ=255) tctTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGAt > 1:1014526/1‑301 (MQ=255) tctTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGAt < 1:328741/301‑1 (MQ=255) ctTTATTGCTAAAATCCTGATCAACAAGGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCTGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATg < 1:491202/301‑1 (MQ=255) ctcttccgatctaTCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATg < 1:1047165/288‑1 (MQ=255) tAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCacgggg > 2:1089460/1‑296 (MQ=255) aTCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGCTgagctcggaagag > 2:1045817/1‑291 (MQ=255) atcacacaaGATATTCTCCCGGGCAAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAAGACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtgc < 2:621536/294‑1 (MQ=255) tCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCCAGCTACTCCTACTCAGCTCATCCAGGCCCAGCCCAAGCAGGAGCCGCAGATAACGGCTTTCACCGCCCAGTTCACCACAAATTCCTACCCATTTGCCCCGCTGATTCCCCGGTGTAACTACTtg > 2:674552/1‑299 (MQ=255) aacaacGATATTCTCCAGCGCCAGCAGTGGACTGACGTCTGCTTCCGGCGTAATGGCGGGTCTTAACGCTTCAATTTCCTGCGCACTGCGGCATTCCCATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCCTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtgctgc < 2:177809/301‑1 (MQ=255) aacaaAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCGGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtgctgc < 1:1096666/301‑1 (MQ=255) aacaaAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtgctg > 1:339221/1‑300 (MQ=255) caaAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATTCCCCGGGGTAACTTTTTCCTGCaa > 2:1187089/1‑301 (MQ=255) caaAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCTCAGTGGTAACTATTTGCTGCaa > 1:270942/1‑301 (MQ=255) aaaGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCCGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATTCGCAGGTGTAACTATTTGCTGCaa > 2:523355/1‑300 (MQ=255) atatTCTGCAGCGCCAGCAGTGGGCGAAAGTCTTCTTCAGGCGTAAAGGCGGTGAGTAACGCTTCAAATTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCGATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACAt < 2:272532/299‑1 (MQ=255) atatTCTCCAGCGCAAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACAt < 1:1090850/299‑1 (MQ=255) aatctcCAGGGCCGGCCGTGGTCGTCCGTCATCTTCCGGCGGAAAGGCGGTTAGTAACGCCTCTATTTCCTGCGCACTGCGGCATTCACATGCC‑GGTGCGCCCAGTTCCCGACACGCCTCGTGATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGGGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAATCATTAGCGGCAGATAACGGCGTACACCGCCCAGTTCACCGCAAATGCCTACCCATGTGCCCCGCTGATGCTCAGTGGTAACTATTTGCTGCAACATgcgc < 2:292582/299‑1 (MQ=255) gcCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTTACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCCGGAACGATg > 1:587158/1‑301 (MQ=255) ctcttccgatctcGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCg < 1:111055/288‑1 (MQ=255) ggCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCgg > 1:1082621/1‑301 (MQ=255) aaCGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCCATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGtt < 1:702500/300‑1 (MQ=255) aCGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTAACCGCCGGATTACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTACCCGCAAATGCCTCCCCATTTTCCCCGCTGTCGCGAAGTGGGAACTATTTGCTTCAACACGCGCAGGCACGATGGCGAAATCGGGTtat > 1:978091/1‑301 (MQ=255) cGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCCGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATTGCGAGATCggaagcg > 2:110961/1‑295 (MQ=255) ttcCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAgcggc < 1:493796/301‑1 (MQ=255) gcgtcagtgcggggcgtacacgcgcAATTCCCTCCTCACTGCTGCATTCACATGCCCGGCGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCCTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGa < 2:489783/274‑1 (MQ=255) ccGTAAAGGCGGTGGGTAACGTTTCAATTTCCTGGGCACTGCGGCACTCACAGGCCGGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATa < 2:338855/299‑1 (MQ=255) tAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATAcgc > 2:191610/1‑300 (MQ=255) ggCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCTTACCCATTTGCCCCGCTGATGCGTAGTGGTAACTATTTGCTGCAACATGCGCAGGACAGAGGCCGTAATCTGGTTATATAGTGGCGATACGCGCggg > 2:336933/1‑301 (MQ=255) cgtgtgctcttccgatctaTTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGt < 1:702978/283‑1 (MQ=255) tGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGttat < 1:927044/300‑1 (MQ=255) gAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGACTACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCCACGCAAATGCCTCCCCATTTGCCCCGCTGATGCGCAGTGGTCACTATTTGCTGCAACATGCGCCGGAACGCTGGCGTAATCGGGTTATCTTGCGGCGATACGCGAGGGttatgt > 2:821844/1‑299 (MQ=255) tctcTTCAATCTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATc < 1:46705/298‑1 (MQ=255) ccTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGa < 1:191824/300‑1 (MQ=255) cTTCAATCTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTTCTGCAACATGCGCAGGAACGCTGGCGTACTCGGGTTATATAGCGGCGATACGCTCGGGGTATTCCGTTcaga > 2:46673/1‑298 (MQ=255) ttCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGAcc < 1:1054570/301‑1 (MQ=255) aaaTTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGc < 1:523932/300‑1 (MQ=255) aaTTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCa < 1:822828/301‑1 (MQ=255) aTTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGtagatcggaagcgcgtcgg > 2:702181/1‑283 (MQ=255) cgcACTGCGGCATTCACATGCCTGACGCG‑CCCGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTTAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATa < 1:46171/300‑1 (MQ=255) cgcACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATa > 1:498429/1‑300 (MQ=255) gcACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATAAGCGCGGGTTATTACGATCGACCGCATACAGATa > 1:770609/1‑299 (MQ=255) ggCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGGTATTACGATCGCCCGCATAAAGCTACTGGGTCat > 2:1068918/1‑301 (MQ=255) ttCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCCGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCg < 1:337299/301‑1 (MQ=255) tgtgaaTGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCg < 2:393096/296‑1 (MQ=255) acaTGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAATCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCACAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCCCCGCAAATGCCTACCCATTTGCCCCGCCGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGCACTCGGGTTATATAGCGGCGATACGCGCGGGTTACTACGCTTCACCGTCTCAAGACACTGGGTCatacactgg > 2:677193/1‑295 (MQ=255) aTGCCTGACGCG‑CCAGTTCCAGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGa < 1:220288/301‑1 (MQ=255) tGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCCGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCCCCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAACCGGGTTATATCGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATCCTGGGTCATATCGTTggcg > 2:842095/1‑299 (MQ=255) cg‑cCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGACCGACCGCATACAGATATAGGGTCATTACGTTGGAGccacta > 2:759627/1‑294 (MQ=255) g‑cCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTga > 1:1050584/1‑301 (MQ=255) ccAGTTCCCGTGACGCCTCGCTATCCAGTTTACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCAAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTgaa < 1:576664/301‑1 (MQ=255) ccacgacgcctttacgatctgaTCCAGTTGACGAAGCTGGTTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCACAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTgaag < 2:1116347/280‑1 (MQ=255) tCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAAt > 1:1062947/1‑301 (MQ=255) cccGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGCTCGACCTCATACCGATACTGGGTCATATCGTTGGGGCCGATACTTAAGAAAt > 2:306632/1‑300 (MQ=255) acacGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGAcc < 1:291970/301‑1 (MQ=255) ccTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGCTGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGCTCCTGGGTCATATCGTTGGTGCCGATACTGAAGAAATCGACCTTAt > 2:244431/1‑300 (MQ=255) ccTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGAccactt > 2:873374/1‑296 (MQ=255) ccTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATc < 1:843079/301‑1 (MQ=255) gtgaaaagccagcttcgtcaacTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCg > 1:239453/22‑301 (MQ=255) cGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGc > 1:1083739/1‑300 (MQ=255) accGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGTCCCAGCCCAAGCACTAGCGTCAGAAAACGGCTTTCACCGCCCAATTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGAccacttctgcgatgaggtcgat < 1:393524/299‑21 (MQ=255) cGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCCGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGCTTCGCCCTGGTACCTATTTGCTGCAACATGCGCAGGAACGCTGGCGCAATCGGGTTATATAGCTGCGCTACCCGCGGGTTATCACGATCTACCGCATACAGATACTTGGTCTCCTCGTTGTTGCCGAAACTGCAGACATCGACCTCATACCAGAAGTCGTCgatgt > 2:216041/1‑300 (MQ=255) cGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCTGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCgatga > 1:201342/1‑301 (MQ=255) cGAAGCTGGCTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGAccacttctgcgatgaggtcgattt < 1:874431/301‑23 (MQ=255) cTTTTCACCGCCGGAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACAGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATAGACCTCATCGAAGACGGGGTCGAGGATGTAGcaaa > 2:608408/1‑299 (MQ=255) ggAATACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACtt > 2:969774/1‑301 (MQ=255) aaTACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTcc < 1:760515/301‑1 (MQ=255) aaTACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTcc < 1:675301/301‑1 (MQ=255) aTACGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCTGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGGGGTCGATGATGTAGCACACCGACGGAACTTcc > 2:1123638/1‑300 (MQ=255) taagcgAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTccac < 1:114381/298‑1 (MQ=255) aaCGCGAGCTAATCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGGAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTccac < 2:645901/300‑1 (MQ=255) ccgcgAGATACTCCTCCCCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCAACGCACAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTAGTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTccacc < 2:270660/300‑1 (MQ=255) aCGCGAGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTccacc < 1:975504/301‑1 (MQ=255) | ACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCG‑CCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCGCCGGAATACGCGGGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTCCACC > NC_000913/2502282‑2502872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |