| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,169,260 | Δ1 bp | 10.4% | intergenic (‑29/+24) | ygiV ← / ← ygiW | transcriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,169,260 | 0 | A | . | 10.4% | 139.3 / 10.5 | 48 | intergenic (‑29/+24) | ygiV/ygiW | transcriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein |
| Reads supporting (aligned to +/- strand): ref base A (21/21); new base . (3/2); total (25/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGC > NC_000913/3168965‑3169554 | cacaCCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAAcgc‑g > 2:377892/1‑301 (MQ=255) aTTTTCGTGGATCGGTTCTCTAACGCTGCCGCAGATATGAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGGCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGG‑ACGC‑GTTCCCGGGaataa < 1:26727/301‑5 (MQ=255) tttCGCTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGAt < 2:210689/300‑1 (MQ=255) cTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACCGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCCCAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGAAGCGGTCACAATTACGGATTTACt > 2:459859/1‑301 (MQ=255) cGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCCACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCATGCGAAATTATTCCCGGAAACGC‑G‑TCCCGGGAGCGGTAACAATTAAGAATTTAcgtaag > 1:338606/1‑296 (MQ=255) gccgATATCAAAGTGAAACGCTTCCGGTGCGGTGGGGTCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTGGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGGGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTg < 1:339361/298‑1 (MQ=255) aGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATCAATTTCGCCACGCTGTAGTTGAGCAATTCACGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCCTTTTGCCTCCCTGCGAACTCATTCCCAGGAACGC‑G‑TCACGGGAGCGGTAACAATACCGGACATACTTAGCGGacacact > 1:129863/1‑295 (MQ=255) gttatCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGttt < 2:242278/299‑1 (MQ=255) atCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGaa > 2:111376/1‑300 (MQ=255) aaaaGTGAATCGCTTCCGGTGGGGTGGTGGCAGGGTCGTCCCAGGCGACTCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGt < 1:377396/300‑1 (MQ=255) gAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGCTCTGTTTGACGTCAAttt > 1:616432/1‑301 (MQ=255) aCGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCaa < 2:347770/301‑1 (MQ=255) gttgggtggtGGGAGGGTCGTCCCAGGCGGCGCCAAAAGTTTGGCTTTGGTTAACAGGAGAATGCCCCGTCGCTGTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCGACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTGTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTcc < 1:459295/297‑1 (MQ=255) cAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCTCAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGa > 2:492130/1‑301 (MQ=255) cccAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCTCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGCTCAGCAATTCAGGCCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTATTTATCGACTTCACCCt > 2:408280/1‑301 (MQ=255) tgACGCTAAAAGTTTGGCTTTGGGTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGTTAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACATGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACCTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCAGGAtcg < 1:407717/300‑2 (MQ=255) gggagcgaaaagttgggtgtgggTTTACATGAGAAATCCCCGGTTGTTTTCGCCACGTGTTAAATTTCGCCACGCTGTAGTTGAGCAATTCTGGGCTACAGCGGTTCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACTATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAAtct < 1:335433/280‑1 (MQ=255) aCGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCa < 2:360963/301‑1 (MQ=255) ccAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAAAAATTACGGATTTACTTTGCGGATCTTTTTGACGTCCATTTCAACAGAATTCCAGTCTTTCACACCTTCACCCTGAATCCCAAcc > 1:51896/1‑301 (MQ=255) gCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCgg < 2:107741/301‑1 (MQ=255) tGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCaa > 2:143419/1‑301 (MQ=255) gATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGACATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGTACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGAGGATCTGTTTGACGTCAATTTCACCAGAATTCCAGTCTTTACCGACTTCACCCTGAATCGCAACCGTAACTTTCGGCTTCACCGTCACGCCGTTcaacccatcgtgcacgatctcaac > 1:68829/1‑278 (MQ=255) tttCGCCACGTTGTAGTTGGGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGAGGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACGTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGAt < 1:530087/301‑1 (MQ=255) ttgtccACGGTGTAGTTGTGCAATTCAGGGCTACTGCGGTGCGGCAACAGCGCCTCAGGGATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATa < 1:491484/297‑1 (MQ=255) caccgctgtagccctgaattgctcaacTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGACCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGGGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTAc > 2:448581/27‑301 (MQ=255) caggttGTAGTTGGGCAATTCAGGGCTAGAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTAcc < 1:111252/296‑1 (MQ=255) gTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCTTCACCCCCTTCCAACCTTTGTGGTCCATAACAACATTTATTGTTCCGCTTGc > 1:11683/1‑301 (MQ=255) tGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCtt < 2:11683/301‑1 (MQ=255) gggCAATTTAGGGCTTCAGGGGTGCGGCAACATCGCCACAGGTAGTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCttt < 1:143293/299‑1 (MQ=255) ggCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACATCAATTTCAACAGCATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTAACGCTGGCATCTTTGAACACGTaga > 1:212678/1‑301 (MQ=255) ttACAGCTGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAgaga < 2:617290/300‑1 (MQ=255) ggTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCag < 2:296285/301‑1 (MQ=255) gTGCGTCAACATCGCCACAGGTATTGATGGGAAATCGATTATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCaga < 2:339069/301‑1 (MQ=255) gCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACg > 2:364257/1‑301 (MQ=255) ccACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAAAGa > 2:568591/1‑301 (MQ=255) gATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAACTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGACCACGTAGAGATCGTCAGAGATGCTTTCAAAGATATTGCCgcgc > 1:626186/1‑301 (MQ=255) cGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTAccc > 2:486242/1‑301 (MQ=255) cgcttttccgttctaGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAgg < 1:546185/287‑1 (MQ=255) aGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAggagatcggaagagca > 2:546858/1‑287 (MQ=255) ttttCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTgcgc < 2:51964/298‑1 (MQ=255) ttGTTATTGTTCGTCCCGGTGTAATTGTTCGCGGGAAGGT‑G‑TTCGGGTATCTGTAAAAATTATGGTTTTAGTTTGAGGATCTGGTGGAAGTCAATGTAAACAGAATTCCAGTCTTTATCGACTTCAACCTGAATCGAAACCGTATCTTTCGGCGTTACCGTCACGCCGTTCCAGCGTTTGTGGTCGTTATCAACATTGATAGTACCGCTGGCATCGATGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTAACGCAGGGATTTTgcgc < 1:549709/301‑1 (MQ=255) cacacgacgctcttccgatctgctCCCGGG‑ACGC‑GTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCt < 1:307790/278‑1 (MQ=255) tttGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATCTCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATCTTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTCCTAc > 1:28949/1‑301 (MQ=255) cGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAACGATCGTAAGAGATGCGTTCAACGATATTGCCGACAAGGGTTACCCAGGTGTCGTCACGCAGGAATTTTGCGCTTTCTACAGTCGTTAAGCt > 1:184092/1‑301 (MQ=255) gctCCCGGG‑ACGCGG‑TCTCGGGAGCGGTAACAATTAAGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTAACTTTCGGCGTCACCGACACTCCGTTCCAGCGTTTGTGTTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGACGGGTTACCCAGGCGACGTCACGAAGGGAATTTGCACTATCTAAAGACGTTACGCTGCCGTTc > 1:388512/3‑301 (MQ=255) gctCCCGGG‑ACGC‑GTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTagatcggaagagcacacatct > 2:308194/3‑281 (MQ=255) cccGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTTCCGTTCGGc > 2:144054/1‑301 (MQ=255) cccGGG‑ACGC‑GTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTAAAGTCGTTACACGGCCGTTCGGc > 2:493684/1‑301 (MQ=255) | CACACCATAACGATTATCGGGAATCGGTTCGCTAACGCTGCCGCAGATATCAAAGCGAAACGCTTCCGGTGCGGTGGTGGCAGGGTCGTCCCAGGCGACGCCAAAAGTCTGGCTTTGGTTAACAGGAGAAAGCCCCGTCTCTTTACGCCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGC‑G‑TCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTCGATATCAACATTGATAGTACCGCTGGCATCTTTGAACACGTAGAGATCGTCAGAGATGCGTTCAACGATATTGCCGCGCAGGGTTACCCAGGTGTCGTCACGCAGGGATTTTGCGCTTTCTACAGTCGTTACGCTGCCGTTCGGC > NC_000913/3168965‑3169554 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |