| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,400,350 | A→T | 6.9% | P402P (CCT→CCA) | nuoF ← | NADH:ubiquinone oxidoreductase, chain F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,400,350 | 0 | A | T | 6.9% | 184.6 / 3.6 | 61 | P402P (CCT→CCA) | nuoF | NADH:ubiquinone oxidoreductase, chain F |
| Reads supporting (aligned to +/- strand): ref base A (30/26); new base T (2/2); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.50e-01 | |||||||||||
TGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCA > NC_000913/2400059‑2400636 | tGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTgg < 2:573976/301‑1 (MQ=255) aGCTAAAGTAAGGAATATCAAGGCCCAGAGACAGATAAGCTTCCAGCATGTTGTCCGCGCCGTTGACCTCGTATTTGTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGTGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCTGGTGcagtggagc < 1:498413/301‑9 (MQ=255) gCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCAC‑GCACCTGGTGcagtggagccg < 2:615408/301‑11 (MQ=255) ttatCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTAccc < 2:266899/300‑1 (MQ=255) agttcagacgtgtgctcttccgatctCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCGCAGAAAGTTTTACCCGGGCCTAAGAATCg < 2:86490/276‑1 (MQ=255) gCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCACCTACACCAGGTAC‑GTGGGACCAGAAAGTTATACCCGAGCCTAAGAATCGACACAGTTGc > 1:346298/1‑301 (MQ=255) ccGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCGCAGAAAGTTTTACCCGGGCCTAAGAATCGagatcggaagagcgtcgtgtagggaa > 1:86366/1‑276 (MQ=255) ttggagttcagacgtgtgctcttccgatcttGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTTTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGccc < 2:338506/271‑1 (MQ=255) ttgtttttCTTTGCTGTCTACATGAATTGTAGCCATTGGATTGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGAcc < 1:628587/295‑1 (MQ=255) cTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCCCAAGTGTTTCGATATCCCCCGGCTGAcc > 1:224617/1‑301 (MQ=255) ttCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTcacc < 2:346761/301‑1 (MQ=255) ttccGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCTACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCATCACCACgac > 2:244294/3‑299 (MQ=255) ccGTTTATATGATTTTTTGGCATTTGCTTGCTTCCAGTTTTCTCATTCAGTGACTTAGCGTTAGTCGAAAGTCGGTTACCTGCGATGTGTCAGCTAGTTCGGCTGAGACCAATTAATCAACTAGGTATTGGTGGACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTcc < 1:244027/301‑1 (MQ=255) cGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTATGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCAACTGCAACAGGTGC‑GTGGGTACAGAAAGTTTTACCCGGGCCTAAGAAGCGACACCGTTGCTACAGTGTTTCGCTATCGACCGGCTGAACTTcaaccagcaccc > 1:589806/1‑291 (MQ=255) tGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTTTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGACAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGAAATCGcccaaatcggaagagaggcgtgtagggaaagag > 1:338043/1‑271 (MQ=255) tGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCAAGagcacga > 2:148533/1‑300 (MQ=255) tAGCCATTAGCTTGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGTATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAAttt < 2:590567/301‑1 (MQ=255) tgCCATTTGCATGTTTCCAGTTTTCTCATTCATAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAAtttt < 2:66007/300‑1 (MQ=255) aGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGACACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCCCCAGCGCACGCAGAATTTTCACGCTc > 1:257062/1‑301 (MQ=255) tGCTTCCAGTTTTCTCAGTCAGAGACTGGGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATAACGCCCGGCTGACCTTCACCACGCTCCAGCCCACGCAGAACTTTCACCCTCCAcc > 1:19509/1‑300 (MQ=255) tGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACg > 2:315448/1‑301 (MQ=255) gattaacgctcAGTCTCTGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGAACGCAAAATTTTCACGCTCAACGGCaaa > 2:95626/9‑299 (MQ=255) cagtcagAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGCATCGACACCGTTGCTCACGTGTTTCGATAACGCCCGGCCGCCCTTCACCACACCCCACCCCACCCAAAATTTTCACGcccccccaccgccccaccccgc > 1:334260/1‑283 (MQ=255) cagtcagAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGc > 2:521226/1‑301 (MQ=255) cagtcagAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCAGACCTTCACCACGCTCCAGCGCACGCAGAATTTTAACGCTCCACGGCAGAACGTccaggc > 1:21143/1‑296 (MQ=255) gatttggCGTTAATTGAAATTTGGTTACCAGCGTTCTTTCAGCAGGTTCGGCGGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTa < 1:315044/295‑1 (MQ=255) aTCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCaa > 2:602675/1‑300 (MQ=255) tttACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGc < 2:19560/300‑1 (MQ=255) gTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCATCCAGGCACGGCGTACACCAGCCGCAGGACACACgggg > 1:255697/1‑300 (MQ=255) ttACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCg > 2:494661/1‑301 (MQ=255) tgtgctcttccgatctagcagGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCg < 2:381850/285‑1 (MQ=255) ccAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGaaa > 2:464850/1‑301 (MQ=255) aGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAACGTTTTACCCGGGCCTACGAATCGACACAGTTGCTCAAGTGTTTCGATCTCGCCCGGCTGACCTTCCCCACGCCCCAGCGCCCGCCGCATTTTCCCGCCCCACGGCACACCGTCGCGGCACGGCATACACCAGCCGCAGCACCCCCGGGCGaaaaa > 1:218663/1‑301 (MQ=255) aGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGaaaaa < 2:558766/301‑1 (MQ=255) tttttAGCTGGTTCGTCTGAATCGCATGAATCAAATGGTTATTGTTGAACGGCTGTTTTAGTCCCGCCCCTCTTTCTTCGCGGAAATATTTTATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGTGCCTAAGACTCGACACAGTTGCTCAAGTTTTTCGATATCGCCCGGCTGACCTTCCCCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTc < 1:400146/300‑1 (MQ=255) ttcagacatgtgcttttcctaTCTCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTc < 2:215981/281‑1 (MQ=255) ttCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACCCTTCCa > 2:218690/1‑301 (MQ=255) agcagGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGACTTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCgcgaccggaagagcgcc > 1:381354/1‑285 (MQ=255) agcagGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGt > 2:278745/1‑301 (MQ=255) gTTCGGCTGAATCCCATGAATCAAATGGGTTTTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGc < 1:601914/301‑1 (MQ=255) gAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGATTACGCACCAGCGa > 2:481157/1‑301 (MQ=255) cATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCATCGCGGCACGGCGTACAACAGCCGCAGGACTCAAGGGAGAAAAActatacaaatccgaaacgcggcgcggaggg > 1:215738/1‑273 (MQ=255) cAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGACACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGAtc < 2:257330/301‑1 (MQ=255) ggTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATAGTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATgg < 1:218447/301‑1 (MQ=255) tGCTTAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAAc < 2:255965/301‑1 (MQ=255) tGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTCACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAACGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCCAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCCGCCGCAGGACTCACGGGCGAAACACTCCTCCAGGTTACGCACAAACCacacaaggctaaacccctgtccaac > 1:243206/1‑280 (MQ=255) gCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGACGCAGGACTCACGGGCGAAAAACCCTTCAAGGTTACGCACCCGCAACACCACGCTACTCTCATGGTCAAcc > 1:500187/1‑301 (MQ=255) cgatctttCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAg < 2:196789/295‑1 (MQ=255) gATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGcc > 2:547538/1‑301 (MQ=255) ttCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCAACGCCacagaacg > 1:196546/1‑294 (MQ=255) cGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTAccc < 2:224860/301‑1 (MQ=255) ccTCAAATTTTTCGCGGAAATTTTTGTGGGCGCTCGGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAg < 1:546865/301‑1 (MQ=255) aaTTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGc < 2:500834/301‑1 (MQ=255) tatTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGACTTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTCCCCGCTTTACCgc > 1:314753/1‑300 (MQ=255) ggggcTGTGTAACGGCTCCACTGTACCAGGTGC‑GTGGGAACAGATAGTTTTACCCGGGCCTAAGAAGCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCGTCACCACGCGCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCg < 1:520554/298‑1 (MQ=255) gggcTTTGTAACGGCTCCACTGGACCAGGGTC‑GTGGGCATAGAAAGTTTTACCCGGGCCGAAGAATCTACACAGTTGCTCAAGGGTTTCGATATCGCCCTGCTGACCGTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGa < 1:480556/299‑1 (MQ=255) tGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCa > 2:414553/1‑301 (MQ=255) tAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATc < 2:142830/301‑1 (MQ=255) tctgtgcccacgCACCTGGTGCAGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGCCTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCCACCGCCATCCCCAACGCCGCACCCAGACGCCTGCCCGCTTTACCGATACTTTCGAACTCCATcc > 1:494044/12‑300 (MQ=255) tgtgcccacgCACCTGGTGCAGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAAtagatcggaa > 2:212643/10‑292 (MQ=255) ggtcccATTGCACCGGGTGG‑TTGGGCACAGAAAGTGTTAACCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCGCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCa < 1:464286/297‑1 (MQ=255) | TGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGC‑GTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCACGGCGTACACCAGCCGCAGGACTCACGGGCGAAAAACTCTTCCAGGTTACGCACCAGCGACACCATGTTGATCTCATGGTCAACCGCCATCGCCAGCGCCGTACCCAGACGGCTGCCCGCTTTACCGATACTTTCGAATTCCATCGGCA > NC_000913/2400059‑2400636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |