Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,464,332	A→T	5.9%	F126L (TTT→TTA)	treC ←	trehalose‑6‑P hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,464,332	0	A	T	5.9%	220.3 / 2.1	73	F126L (TTT→TTA)	treC	trehalose‑6‑P hydrolase
Reads supporting (aligned to +/- strand):  ref base A (38/29);  new base T (2/2);  total (41/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

GTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTA  >  NC_000913/4464041‑4464632                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                             ttAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGTTTTATATCTGGCgc                                                                                                                                                                                                                                                                                                     <  2:545039/300‑2 (MQ=255)   aGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTa                                                                                                                                                                                                                                                                                                   >  2:541354/1‑301 (MQ=255)    gAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAAATGGCGGTaa                                                                                                                                                                                                                                                                                                  >  2:227914/1‑301 (MQ=255)      aGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑AGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAACAAGATGAAACTAGTACTGTTCGATTTCCGCATGCCAGCGCCAAGCACTACCGCACAATTTTGAACGCAAATTGTTCGGTGGCGTTTCAGTTTCACCACCACACCAGATATAAAACTGGcaatacgg                                                                                                                                                                                                                                                                                                >  1:352655/1‑294 (MQ=255)              ccGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGctttctt                                                                                                                                                                                                                                                                                        >  2:213863/1‑301 (MQ=255)              ccGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGctttctt                                                                                                                                                                                                                                                                                        <  2:529588/301‑1 (MQ=255)               cGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAACTAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATAGTGCACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATAAAAAAATAGACGAAAGGGcttacttt                                                                                                                                                                                                                                                                                       >  1:579325/1‑301 (MQ=255)                   gCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTAACGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATTGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCCGTTGATCGGTGGCGTTTCTGGTTCTCCATCGCGCAAAGAATAAAACTGGAGGTAAGGGcctactatgatc                                                                                                                                                                                                                                                                                   >  1:132934/1‑290 (MQ=255)                    ccGTCTAGGTCGTCATGGAAACGCGTGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCa                                                                                                                                                                                                                                                                                  <  2:353118/301‑1 (MQ=255)                        ccAGGTCTTCAGGGAAACGCGTGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAgcgc                                                                                                                                                                                                                                                                              <  2:258315/301‑1 (MQ=255)                         cAGGTCTTCAGGGAAACACGGCTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GCCCGGCCCAGAACTCACACACTTTTTTCCGCTCTCCGCGTACCGC‑TCGCTTCTCCCAGTTGAGCTACGCCTGTCCTGGTGCCAAGAGATGCACATAGTACTCTCCGCCTTCCGCATGCCAGCGCCACGCCCTCCCGCAAAACTTTGCACCCCTGTCGTTCGATGGCGTTTCTGGTCAACCATCACACCATATATACAACTGGCGCTAAGGCCCTTCATTGCTCAGCGcc                                                                                                                                                                                                                                                                             >  1:113757/1‑301 (MQ=255)                                tCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGaa                                                                                                                                                                                                                                                                      <  2:393132/301‑1 (MQ=255)                                             ggTCTTTGGAGATCAGATTCACCACCTCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTg                                                                                                                                                                                                                                                         <  1:227671/301‑1 (MQ=255)                                              gTCTTTGGTGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTgg                                                                                                                                                                                                                                                        >  1:494007/1‑301 (MQ=255)                                              gTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAACGCCTCGCGAAACCAGGCAGGTTgg                                                                                                                                                                                                                                                        >  1:526326/1‑301 (MQ=255)                                                ttttcccgtcttggtttaacacaACCAAGCTCAAACCGTTGACCCCACGGACCGGCCCAG‑ACTTAAAGACTTTTTTTAGGTCTGGGCGTTCCGCTTGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTggg                                                                                                                                                                                                                                                       <  2:515502/282‑1 (MQ=255)                                                    ggAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCCAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGCTATAAAACTGGCGGGAAGGGCTTTCTTTGTTCAACGCCTCGCCAAACCAGGCATGTTGGGTcgag                                                                                                                                                                                                                                                  >  1:636229/1‑301 (MQ=255)                                                             aTTCCCCCCATCCAGGCGCAACCCGTCGACCCCAC‑GTTCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTa                                                                                                                                                                                                                                         <  1:306513/301‑1 (MQ=255)                                                                caccacATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCTCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTaaaa                                                                                                                                                                                                                                      >  2:20145/1‑300 (MQ=255)                                                                    acaTCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCa                                                                                                                                                                                                                                  <  2:189399/301‑1 (MQ=255)                                                                     caTCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGATAAACACCaa                                                                                                                                                                                                                                 >  2:572524/1‑300 (MQ=255)                                                                          aGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCga                                                                                                                                                                                                                            >  2:264671/1‑301 (MQ=255)                                                                              gcAACCCGTCGTCCCCAC‑GATCGGCCCAGAACTCACAGGCGTTTTTCAGCTCTGCGCGTACCGC‑TGGATCTTCCCAGTTGAGATCCGCCTGTTCTGGGGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAAt                                                                                                                                                                                                                        <  1:205978/301‑1 (MQ=255)                                                                                               cctaTCTGCCTAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTCTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCcgcgc                                                                                                                                                                                                        <  2:133059/298‑1 (MQ=255)                                                                                                           gAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGAGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCAAAACGAGAATAAAACAAATCCCGCACGATTTTGCCTGc                                                                                                                                                                                            >  1:115430/1‑301 (MQ=255)                                                                                                                cacaGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAACTTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCCGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATCCGAATCCCCCGCACATTTGCCTGCGCCAc                                                                                                                                                                                       >  1:456627/1‑301 (MQ=255)                                                                                                                cacaGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGCAACGAATCCCCCGCGATTTTGCCTGCATCAc                                                                                                                                                                                       >  1:373518/1‑301 (MQ=255)                                                                                                                 acaGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCAcc                                                                                                                                                                                      <  2:526998/301‑1 (MQ=255)                                                                                                                      cTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTc                                                                                                                                                                                 >  2:437805/1‑301 (MQ=255)                                                                                                                       ttttttCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCg                                                                                                                                                                                <  2:115554/301‑1 (MQ=255)                                                                                                                          tttCAGCTCTGGGGGTACCGC‑TGGATTCTCCCGGTTGAGATCGTTCTTTTCTTGGGAAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCGAATGTAGAACGCCAGTCGTTCGGGGGTGTGTCTGGTGCGTCAGCGGGGCAGAAATAACACTGGCGGTAAGGGATTTCTTTGTTCAGCACCAGGAGAAACCAGGCATGTTGGGTAGAGGTAAGGTTAAACACCATATCGAGAAGGATACGAATCCCGCGCGATTGTGCCTGCGTCACCAGTTCGtga                                                                                                                                                                             <  1:429657/301‑3 (MQ=255)                                                                                                                             cAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAACCCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTCCCTGCGTCCCCAGTTCGTCaaaa                                                                                                                                                                          >  1:173558/1‑301 (MQ=255)                                                                                                                                         tACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTTTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACGAGTTCGTCAAAATCGTCCAGCGTg                                                                                                                                                              <  1:264403/301‑1 (MQ=255)                                                                                                                                                       tcCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGAt                                                                                                                                                 <  2:494653/301‑1 (MQ=255)                                                                                                                                                        cccAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATc                                                                                                                                                >  1:283010/1‑301 (MQ=255)                                                                                                                                                                agaTCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCg                                                                                                                                        <  2:457191/301‑1 (MQ=255)                                                                                                                                                                  aTCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGta                                                                                                                                      >  2:570729/1‑301 (MQ=255)                                                                                                                                                                               ggTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCCCCAGTTCGTCCCAATCGTCCAGCGTGCCGTAGGAGGGATCACACGCCGTATAGTTCGCtcctc                                                                                                                         >  1:84733/1‑297 (MQ=255)                                                                                                                                                                               ggTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGACACAATCGCCgacaaattcaccacgt                                                                                                                         >  1:66440/1‑286 (MQ=255)                                                                                                                                                                                 ggaaaaaggattgtaaTTGTACTGTTCGCTTTCCGTATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTAcgtcg                                                                                                                       <  1:540681/287‑1 (MQ=255)                                                                                                                                                                                                      cTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGga                                                                                                  >  2:72406/1‑301 (MQ=255)                                                                                                                                                                                                           cGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCATTATCGACCTGGGgagagaa                                                                                             >  2:312223/1‑300 (MQ=255)                                                                                                                                                                                                              tttCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATa                                                                                          >  2:213930/1‑301 (MQ=255)                                                                                                                                                                                                                  cGCATGCCTGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCTCGATTTTGCCTGCGTCACCCGTTCGTCAAAATAGTCCCGCGTGCCGTAGGTGGGAACAATCGCCGTATCGTTCGCTACCGCCACACCGTTCTCGCCCGTGGGAGAGCCATaacaa                                                                                      >  1:1130/1‑298 (MQ=255)                                                                                                                                                                                                                       gCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGAAATAAAAGGGGGt                                                                                 >  2:208057/1‑301 (MQ=255)                                                                                                                                                                                                                       gCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATAACGAGAATGATACGAATCCCGCGCGAATTTGCCTGCGTCACCAGTTCGTCAAAATCGTACAGCGTGCCGTAGGAGGGATCAATCGACGTATAGTTAGCTACGTAGTAACCGTAATAGAACTGGGGAGAACCAAAACAGAGGGt                                                                                 >  1:125302/1‑301 (MQ=255)                                                                                                                                                                                                                        ccAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGtt                                                                                >  2:161723/1‑301 (MQ=255)                                                                                                                                                                                                                         tttcagacgtttgctcttccgttctttGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGA‑TTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAg                                                                              <  2:183978/277‑1 (MQ=255)                                                                                                                                                                                                                                    aCTACCTCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCa                                                                    <  2:84857/301‑1 (MQ=255)                                                                                                                                                                                                                                     ttACCGCTAAATTTTGAACGCTAGTTGTTCGGTGTCGTTTCTGGTTCTCCATCGCGCCAGATATAGTACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCAt                                                                   <  2:125426/300‑1 (MQ=255)                                                                                                                                                                                                                                                 tttGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTCAAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAAAGACATAAAAGGGGGTTAGCCACAAGACAACAACGCACAgat                                                       >  1:134409/1‑299 (MQ=255)                                                                                                                                                                                                                                                  ttGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGA‑TTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAgagaacggaagagcgacgtgtagggg                                                     >  1:183737/1‑276 (MQ=255)                                                                                                                                                                                                                                                   tGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTa                                                     <  2:66530/301‑1 (MQ=255)                                                                                                                                                                                                                                                     attgCCGGTGGTTCTGTGGCGTTTCTGGTTTTCCATCGTGCCAGATATAAAACTTGCGGTAAGGGCCTTCTTGGTTCTGGGCCTCGCGAAACCATGCATGTTGGGGAGAGGTATGGTTAAAGTCCATATCGAGAATGTTGCGAAGCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTAGAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGATATAAAAGGGGGTTAGGCAGATGGCATCAACGCCCAGTGTaag                                                   <  1:20094/298‑3 (MQ=255)                                                                                                                                                                                                                                                     aaCGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCACAGTTTATg                                                   >  2:256894/1‑301 (MQ=255)                                                                                                                                                                                                                                                      acgcatgttgttCGGTGGTTTTTTTTGTTTTCCATCTCGCCAGTTATAAAACTGGCGGTAAGGGCTTTCGTTGTTCGTCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGc                                                  <  1:256626/295‑1 (MQ=255)                                                                                                                                                                                                                                                       cGCCAGTTGTTCTGTGGCGTTTCTGTTTCTCCTTCGCGCCAGTTATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCATTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCa                                                 <  1:311819/301‑1 (MQ=255)                                                                                                                                                                                                                                                         ccaccgaacaactGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCNTCACCAGNNNNNNAAAATCGTCCAGNGTGCCGTAGGTGGGATCANACGCCGTATAGTTNNCTACGTCGTAACCGTAATCAACCTGGGGAGCAACATAAAACGGAGGACACCAGAAGGCAACAACCCACAgacgatgcaaa                                               >  1:268444/13‑291 (MQ=255)                                                                                                                                                                                                                                                                ttCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAAAttgaccc                                        >  1:108222/1‑295 (MQ=255)                                                                                                                                                                                                                                                                      ggCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAATCCAGGAGtt                                  >  2:358743/1‑301 (MQ=255)                                                                                                                                                                                                                                                                       gCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTg                                 <  2:173755/301‑1 (MQ=255)                                                                                                                                                                                                                                                                        ggTTTCTGGTTTGTCATCGCGCCAGATATAAAACTGGCGTTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTgg                                <  2:402281/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         ttttCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGa                               <  2:637106/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             tttgTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAAc                           <  2:134534/298‑1 (MQ=255)                                                                                                                                                                                                                                                                             cTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATaaa                           >  2:507646/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              ccttcgggtttaccgcCAGTTTTATATCTGGCGGTAAGGGCTTTCTGTGTTCAGCGCCTCGCGAAACCAGGCATTTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCTGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACg                          <  1:399064/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                gTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGAATAACGcc                        >  2:604098/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                 ttCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCa                       <  2:580049/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tttcCATCGCGCCAGTTATAAAGCTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCAc                      <  1:161572/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                     ccATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTa                   >  1:224343/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                      cttaccgcCAGTTTTATATCTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCAAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTaa                  >  2:270732/6‑301 (MQ=255)                                                                                                                                                                                                                                                                                         accgcCAGTTTTATATCTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCTGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACgagatcggaaga               >  2:399578/3‑290 (MQ=255)                                                                                                                                                                                                                                                                                          gcgcCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCAACCTGGGGAGAGACCTAAACAGGGGTTAGCCAAATGCCATCAACGCACAGTTAATGCAGATCGTCCAGGTGCAGGCTAACGcaccgcaacccc              >  1:373942/1‑290 (MQ=255)                                                                                                                                                                                                                                                                                                    aaaaaGTGGCGGTAAGGGCTTTTTTTGTTCGGCGCCTCGCGAAACCAGGCATGTTGGGTAGGGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGc    <  1:213687/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     aaaaCTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCCTGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGCTACGAATCCCGCGCGCTTTTGCCTGCGTCACCAGTTCGTCAAACTCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTAAGTCGTACCCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTACAGGTGTTGGATAACGCCActaacatagcaggtaacgac   >  1:422316/1‑282 (MQ=255)                                                                                                                                                                                                                                                                                                      aaaCTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATTGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTa  <  2:108346/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                             GTAGAAGCGACGCCCGTCGCCGTCCAGGTCTTCAGGGAAACGCGGGTCTTTGGAGATCAGATTCACCACATCCAGGCGCAACCCGTCGACCCCAC‑GATCGGCCCAGAACTCACAGACTTTTTTCAGCTCTGCGCGTACCGC‑TGGATTCTCCCAGTTGAGATCCGCCTGTTCTGGTGCAAAGAGATGCAAATAGTACTGTTCGCTTTCCGCATGCCAGCGCCACGCACTACCGCCAAATTTTGAACGCCAGTTGTTCGGTGGCGTTTCTGGTTCTCCATCGCGCCAGATATAAAACTGGCGGTAAGGGCTTTCTTTGTTCAGCGCCTCGCGAAACCAGGCATGTTGGGTAGAGGTATGGTTAAACACCATATCGAGAATGATACGAATCCCGCGCGATTTTGCCTGCGTCACCAGTTCGTCAAAATCGTCCAGCGTGCCGTAGGTGGGATCAATCGCCGTATAGTTCGCTACGTCGTAACCGTTATCGACCTGGGGAGAGACATAAAAGGGGGTTAGCCAGATGGCATCAACGCCCAGTTTATGCAGATAGTCCAGGTGTTGGATAACGCCACGTAAATCGCCGGTACCGCTA  >  NC_000913/4464041‑4464632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑