Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,997,525	0	A→G	100.0%	0.8	1	F26L (TTT→CTT)	insC1	IS2 repressor TnpA
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/1):  ref base (0/0):  total (0/1)

CCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTG  >  NC_000913/2997514‑2997713            |                                                                                                                                                                                             ccccGGTTCAAGGCTCTTCTGAACAATTGCGATCTTTTCCTGTGTGGTCCGCCTTCTGCTTTTCTCCGTCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTTGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGgtg  <  1:629162/200‑1 (MQ=14)            |                                                                                                                                                                                             CCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACTTGCTCTTTTCTTCTGGTG  >  NC_000913/2997514‑2997713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.