Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,781,1400A→G12.2% 16.6 ‑37.9 0.0183intergenic (+123/‑75)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Rejected: Polymorphism prediction not supported by minimum number of reads on both strands.
Rejected: Prediction has biased read strand distribution supporting polymorphism.
Reads supporting (aligned to +/- strand):  new base (1/21):  ref base (144/14):  total (148/35)
Fisher's exact test strand distribution p-value = 3.50e-17
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

GTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGAC  >  NC_000913/3780952‑3781334
                                                                                                                                                                                              |                                                                                                                                                                                                      
gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTg                                                                                                                                                                                            >  1:536189/1‑200 (MQ=255)
           gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAgg                                                                                                                                                                              >  1:311651/1‑200 (MQ=255)
            ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGc                                                                                                                                                                             >  1:872972/1‑200 (MQ=255)
                                aCTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTTTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTc                                                                                                                                                         >  1:554384/1‑200 (MQ=255)
                                        ccAGGGAGAAAGGGAGTGCGGCATGTTCTTTGCCCCCCCCTCACCCAACCCCTCTCCCTCGGGGAGGGGGGCCGGTGGGGCTTTTTATGT‑CCCCCCTCCCTCAAAACCCCCACCCCATTTTTTTTTTCCCCCCCCCCCCGC‑GGGGGAGGGGGCG‑GGGGGGGGGG‑‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                                                                                 <  1:687976/200‑1 (MQ=255)
                                                  aGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAgg                                                                                                                                                                                                      >  2:203205/1‑141 (MQ=255)
                                                   gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAggg                                                                                                                                                                                                     <  2:736649/141‑1 (MQ=255)
                                                    gagacgcgggaatggtttttGCCCCCCCCCCCCCCAACCCCCCCCCCCGGGGGGGGGGGGCC‑TTCGGGGCTTTGTATGACCCCCCCACCCTTAAACCACACCCCCCTTTTTTTTTCCCCCCCCCCCCCC‑CGGGGGGGGGGCG‑GGGGGGGGGG‑‑GAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                                                                                                                      <  1:203474/183‑1 (MQ=255)
                                                     gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGcc                                                                                                                                                                                                   >  2:806149/1‑141 (MQ=255)
                                                            gCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAggg                                                                                                                                                                                                     >  3:79125/1‑132 (MQ=255)
                                                            gCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAggg                                                                                                                                                                                                     >  3:508709/1‑132 (MQ=255)
                                                               tAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGagagagagaaaaaaagag                                                                                                                                                                                         <  2:374314/141‑15 (MQ=255)
                                                                     tttGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑TCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                                                                                                    >  1:561975/1‑200 (MQ=255)
                                                                     tttGCCCCCCCTCACCCTAACCCTCTCCCTCAGGGGGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAg                                                                                                                                                                               <  2:413448/141‑1 (MQ=255)
                                                                             cccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑aaaa                                                                                                                                                                                >  3:720761/1‑132 (MQ=255)
                                                                             cccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCaa                                                                                                                                                              >  4:671528/1‑150 (MQ=255)
                                                                             cccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                                                                                                            >  1:536972/1‑200 (MQ=255)
                                                                             cccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGCGAGCGGGCC‑GGGGCGAGGG‑‑G‑aaaa                                                                                                                                                                                >  3:552765/1‑132 (MQ=255)
                                                                                 cACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCCGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACtt                                                                                                        >  1:570419/1‑200 (MQ=255)
                                                                                   cccTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCt                                                                                                                                                        >  4:509388/1‑150 (MQ=255)
                                                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAg                                                                                                                                                                               >  2:709305/1‑127 (MQ=255)
                                                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGc                                                                                                                                                                          >  3:198559/1‑132 (MQ=255)
                                                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCAc                                                                                                                                                                 >  2:282921/1‑141 (MQ=255)
                                                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCAc                                                                                                                                                                 >  2:49410/1‑141 (MQ=255)
                                                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCt                                                                                                                                                        >  4:178872/1‑150 (MQ=255)
                                                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCt                                                                                                                                                        >  4:203253/1‑150 (MQ=255)
                                                                                         cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAAttt                                                                                                                                                           >  2:507120/1‑141 (MQ=255)
                                                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCaa                                                                                                                                                              >  3:705214/1‑132 (MQ=255)
                                                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCaa                                                                                                                                                              >  3:810238/1‑132 (MQ=255)
                                                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                                                                                                                     >  2:425742/1‑141 (MQ=255)
                                                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                                                                                                                     >  2:640448/1‑141 (MQ=255)
                                                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                                                                                                                     >  2:619528/1‑141 (MQ=255)
                                                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAAc                                                                                                                 >  1:146008/1‑177 (MQ=255)
                                                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTCTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTTCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTACACATCGTACTTTACGAACTAGAAAt                                                                                          >  1:1171/1‑200 (MQ=255)
                                                                                                 cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCCCTCCTCACCAATTTTCTGCTATCCTGccccc                                                                                                                                          >  4:462656/1‑150 (MQ=255)
                                                                                                 cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTAt                                                                                                                                                   >  2:863290/1‑141 (MQ=255)
                                                                                                   cAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAAtttt                                                                                                                                                          >  3:340070/1‑132 (MQ=255)
                                                                                                     gggAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGcc                                                                                    >  1:750615/1‑200 (MQ=255)
                                                                                                       gagaGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                                                                                                    >  4:732873/1‑150 (MQ=255)
                                                                                                         gagGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTa                                                                                                                                                    >  3:373783/1‑132 (MQ=255)
                                                                                                           ggggACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑AGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                                                                                                                  >  3:580553/1‑132 (MQ=255)
                                                                                                             ggACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCCCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGCGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                                                                                                              >  4:913627/1‑150 (MQ=255)
                                                                                                                  gTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                                                                                 >  4:251572/1‑126 (MQ=255)
                                                                                                                  gTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAg                                                                                                                                  >  2:902472/1‑141 (MQ=255)
                                                                                                                       gcgcTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                                                                                                    >  4:760542/1‑150 (MQ=255)
                                                                                                                       gcgcTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCCC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                                                                                                                      >  3:395292/1‑132 (MQ=255)
                                                                                                                            gtatgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                                                                                 >  3:473230/1‑132 (MQ=255)
                                                                                                                            gtatgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                                                                                 >  3:558269/1‑132 (MQ=255)
                                                                                                                            gtatgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                                                                                 >  3:751203/1‑132 (MQ=255)
                                                                                                                            gtatgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg                                                                                                                                 >  3:252739/1‑132 (MQ=255)
                                                                                                                            gtatgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                                                                                                        >  2:16151/1‑141 (MQ=255)
                                                                                                                            gtatgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                                                                                                        >  2:182597/1‑141 (MQ=255)
                                                                                                                            gtatgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTGCGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTc                                                             >  1:393358/1‑200 (MQ=255)
                                                                                                                            gtatgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTACGCCACATACTGGCAACATCATCCGTc                                                             >  1:384041/1‑200 (MQ=255)
                                                                                                                            gtatgt‑aCCCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCCCGCCCCTC‑CGGGGCGCGGGCC‑GGGGCGAGGG‑‑G‑AAAAGCCCGCACTGCTCACCACTCTTCTGCTATCCCGCACCCGCAccgatg                                                                                                                                 >  3:900108/1‑127 (MQ=255)
                                                                                                                             ttatgcccccccTCCCTCTAAACCACCCCCGCATTCTTTTTTCCCCCCCCCCCCCGG‑GGGAGAGGGGGCG‑GGGGTGGGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                                                                                                                        <  2:496912/132‑1 (MQ=255)
                                                                                                                              atgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                                                                                                                               >  3:886309/1‑132 (MQ=255)
                                                                                                                              atgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCg                                                                                                             >  4:557128/1‑150 (MQ=255)
                                                                                                                                 t‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                                                                                                                   >  2:718748/1‑141 (MQ=255)
                                                                                                                                    cTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                                                                          >  3:106149/1‑132 (MQ=255)
                                                                                                                                    cTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgcgc                                                      >  1:101788/1‑200 (MQ=255)
                                                                                                                                      cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAAc                                                                                                                 >  1:66365/1‑139 (MQ=255)
                                                                                                                                      cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAt                                                                                                               >  2:469485/1‑141 (MQ=255)
                                                                                                                                      cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa                                                                                                      >  4:812693/1‑150 (MQ=255)
                                                                                                                                        cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGTCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg                                                                                                    >  4:394767/1‑150 (MQ=255)
                                                                                                                                            ctctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                                                                                 >  3:276675/1‑101 (MQ=255)
                                                                                                                                            ctctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                                                                                                                  >  3:858085/1‑132 (MQ=255)
                                                                                                                                            ctctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGCCCGCACTGCTCACCAATTTTCTGCTATCCTACCCCCGCACTACGGGGGCATTATGCTaac                                                                                                                  >  3:12099/1‑131 (MQ=255)
                                                                                                                                             gcggAACCGACGCCGCGCTTTTTTTTCCCCCCTC‑CCCCCC‑GGGGGGGGGGGCCGGGGGGGAGGG‑‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAAc                                                                                                                 <  3:687407/129‑1 (MQ=255)
                                                                                                                                                taaacaccacccccccctTTTTTTCCCCCCCCCCCCCCAGGGGGAGAAGGGC‑GGGTTGGGGG‑‑GTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTGGCGCTAATACCGGc                                            <  1:359879/184‑1 (MQ=255)
                                                                                                                                                      cacccccctcctTTTTTTCCCCCCCCCCCCCCGGGGGGAGAGGGCG‑GGGGGGGGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTc                                     <  1:342663/190‑1 (MQ=255)
                                                                                                                                                      acacCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGTTGCACTGCTCACCAATTTTCTGCTATTCTGCTCTCGTACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCa                                                                                               >  2:450036/1‑141 (MQ=255)
                                                                                                                                                      acacCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACtt                                                                                                        >  3:571526/1‑132 (MQ=255)
                                                                                                                                                             tcttttttttcCCCCCCCGCCCGTG‑GGGGGGGGGGGCG‑GGGGAGGGGA‑‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATatc                             <  1:261872/196‑1 (MQ=255)
                                                                                                                                                              ctctTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc                                                                                       >  2:128959/1‑141 (MQ=255)
                                                                                                                                                               tttttttttCCCCCCCCCCCCGG‑GGGGGAGGGGGCG‑GGGGTGGGGA‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATatcat                           <  1:622983/198‑1 (MQ=255)
                                                                                                                                                                ctTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTACGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                                                                              >  3:340564/1‑132 (MQ=255)
                                                                                                                                                                ctTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                                                                              >  3:833627/1‑132 (MQ=255)
                                                                                                                                                                ctTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTg                                                                            >  4:345437/1‑150 (MQ=255)
                                                                                                                                                                ctTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATatcatc                          >  1:442461/1‑200 (MQ=255)
                                                                                                                                                                ctTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATatcatc                          >  1:821328/1‑200 (MQ=255)
                                                                                                                                                                ctTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGGGAGGG‑‑G‑AAAAGGCCGCTCTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAATCAGAAATTCCGCCAACTACtt                                                                            >  4:819362/1‑149 (MQ=255)
                                                                                                                                                                 tttttttCCCCCCCCCCCCCC‑TGGGGAGAGGGGG‑AGGGTGTGGGGAG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATatcat                           <  1:922847/200‑1 (MQ=255)
                                                                                                                                                                    ttcccccccCCGCCCCCG‑GGGGGGGGGGGGG‑GGGGGGGGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAAc                      <  1:472704/194‑1 (MQ=255)
                                                                                                                                                                    tgtcgcccccccccccGC‑CGGGGGAGGGGCG‑GGGGGGGGGG‑‑GCAAAAGGCCGCACTGCTCACCAATTTTCGGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa                                                                                           <  3:905842/120‑1 (MQ=255)
                                                                                                                                                                          cccTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGTCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGcc                                                                                    >  3:33244/1‑132 (MQ=255)
                                                                                                                                                                          cccTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                                                                           >  2:30920/1‑141 (MQ=255)
                                                                                                                                                                           ccTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCa                                                                                   >  3:428832/1‑132 (MQ=255)
                                                                                                                                                                            ccccccccCC‑CGGGGGGGGGGCC‑GGGGGGGGGG‑‑GAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc                                                                 <  4:570365/146‑1 (MQ=255)
                                                                                                                                                                             cccccccCC‑CGGGGGGGGGGCCGGGGGTGGGGG‑‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCa                                                                         <  2:246934/138‑1 (MQ=255)
                                                                                                                                                                              cGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGt                                                              >  4:575936/1‑150 (MQ=255)
                                                                                                                                                                               cccccTC‑GGGGGGGGGGGCG‑GGGGTGGGGG‑‑A‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTc                                                             <  4:474503/149‑1 (MQ=255)
                                                                                                                                                                               cccccCC‑CGGGGGGGGGGGC‑GGGGGGGGGG‑‑GAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGAtt            <  1:468783/199‑1 (MQ=255)
                                                                                                                                                                                  ccTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCGAATACTGGCAAcatca                                                                   >  2:531999/1‑141 (MQ=255)
                                                                                                                                                                                  ccTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt                                                          >  4:439276/1‑150 (MQ=255)
                                                                                                                                                                                  ccTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGAGATTTGcc        >  1:753616/1‑200 (MQ=255)
                                                                                                                                                                                   ccgcgggggGGGGGGCG‑GGGGTGGGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGcc        <  1:91366/195‑1 (MQ=255)
                                                                                                                                                                                    tC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc                                                                          >  3:627745/1‑132 (MQ=255)
                                                                                                                                                                                    tC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACACCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc                                                                          >  3:862736/1‑132 (MQ=255)
                                                                                                                                                                                             gagGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATAc                                                >  4:37053/1‑150 (MQ=255)
                                                                                                                                                                                              |                                                                                                                                                                                                      
GTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTC‑CGGGGAGAGGGCC‑GGGGTGAGGG‑‑G‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGAC  >  NC_000913/3780952‑3781334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.