Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,222,566	C→A	intergenic (+117/‑67)	ebgR → / → ebgA	transcriptional repressor/evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,222,566	0	C→A	100.0%	233.9	61	intergenic (+117/‑67)	ebgR/ebgA	transcriptional repressor/evolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  new base (29/32):  ref base (0/0):  total (29/32)

CTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTC  >  NC_000913/3222393‑3222756                                                                                                                                                                              |                                                                                                                                                                                               cTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCAt                                                                                                                                                                      <  1:219327/200‑1 (MQ=255)          ttAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGaa                                                                                                                                                             <  1:38287/200‑1 (MQ=255)          ttAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGaa                                                                                                                                                             <  1:595141/200‑1 (MQ=255)                           cAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCGGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATc                                                                                                                                                                                              <  4:829570/150‑1 (MQ=255)                              aTTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTAc                                                                                                                                         <  1:829539/200‑1 (MQ=255)                                                gACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTAc                                                                                                                                                                         <  4:445825/150‑1 (MQ=255)                                                  cccGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTacac                                                                                                                                                                                <  2:180270/141‑1 (MQ=255)                                                  cccGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCa                                                                                                                                                                       >  4:503518/1‑150 (MQ=255)                                                     gTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCa                                                                                                                                                                    >  4:865111/1‑150 (MQ=255)                                                             cccTTACACACTGTCGGGCAATAGTTTTTGCCGGACAGTGCTCCCGTTTATTTTCGTGATCCAGTAAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATACCCAGCAATTAcacac                                                                                                                                                                              >  3:264219/1‑131 (MQ=255)                                                             cccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATc                                                                                                                                                                     >  2:314206/1‑141 (MQ=255)                                                              ccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACaaa                                                                                                                                                                             >  3:564773/1‑132 (MQ=255)                                                              ccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATg                                                                                                                                                           <  4:665128/150‑1 (MQ=255)                                                              ccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATg                                                                                                                                                           >  4:550958/1‑150 (MQ=255)                                                              ccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATg                                                                                                                                                           <  4:501723/150‑1 (MQ=255)                                                              ccTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATc                                                                                                                       <  1:551010/186‑1 (MQ=255)                                                               cTTACACACTGTCCGGCAGTCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATgg                                                                                                                                                          >  4:343236/1‑150 (MQ=255)                                                               cTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAATTAAATGCATTTACCTGCTACTTTTTAGTAATATTTTTACTAAAATCCCCAGCAATTACACTAACTACCATCAc                                                                                                                                                                   >  2:713461/1‑141 (MQ=255)                                                               cTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCAc                                                                                                                                                                   >  2:144816/1‑141 (MQ=255)                                                               cTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATgg                                                                                                                                                          >  4:240275/1‑150 (MQ=255)                                                               cTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAAGGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAAc                                                                                                                                                                            >  3:205212/1‑132 (MQ=255)                                                                      acTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAAt                                                                                                                                                            <  2:558967/141‑1 (MQ=255)                                                                       cTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAcc                                                                                                >  1:891845/1‑200 (MQ=255)                                                                         gTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCAcc                                                                                                                                                                  >  3:558282/1‑132 (MQ=255)                                                                         gTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTtc                                                                                                                                                >  4:552347/1‑150 (MQ=255)                                                                          tCCGGCAATCGTTGTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCa                                                                                                                                                                 <  3:550395/132‑1 (MQ=255)                                                                            cGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTCCTATCACCATg                                                                                                                                                               >  3:278540/1‑132 (MQ=255)                                                                               cAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAAt                                                                                                                                                            <  3:310810/132‑1 (MQ=255)                                                                                aaTCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATg                                                                                                                                                           <  3:16326/132‑1 (MQ=255)                                                                                     tttttGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCATTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGa                                                                                                                                    <  4:191577/150‑1 (MQ=255)                                                                                          gCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCAGTTACCTGCTACTTATTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgcgc                                                                             <  1:240281/200‑1 (MQ=255)                                                                                             ggACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCggg                                                                                                                                     <  2:782804/141‑1 (MQ=255)                                                                                                cAGTTCTGCCGTTTATTTTCGTGATCCAGCTAAAGTAAATGCATTTACCTGCTACTTTTTCGTAAAACTTTTACTACCATCCCCAGCTATTCCACAAACTCCCATCACCCTCAATTTTTCCGATTTctccct                                                                                                                                           >  3:892942/1‑132 (MQ=255)                                                                                                  gTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCAc                                                                                                                                                                   >  2:554456/1‑106 (MQ=255)                                                                                                  gTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCAc                                                                                                                                                                   <  3:554439/106‑1 (MQ=255)                                                                                                  gTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTAc                                                                                                                                         >  3:643081/1‑132 (MQ=255)                                                                                                  gTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCgcgtgcg                                                                     >  1:466096/1‑200 (MQ=255)                                                                                                  gTGCTGCCGTTAATTTTCGTGATCCAGTTAAAGTGAATGCATTTACCTACTACTTTTTAGTAGAAATTTTACTGAAATCCCCAGCAATTACACGAACGACCATCACCATGAATGGTTCCGATTTCTCTCTAc                                                                                                                                         <  3:134305/132‑1 (MQ=255)                                                                                                     ctgcCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTAc                                                                  <  1:682060/200‑1 (MQ=255)                                                                                                          gTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAAt                                                                                                                        <  2:177575/141‑1 (MQ=255)                                                                                                                 ttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGa                                                                                                                          <  3:528676/132‑1 (MQ=255)                                                                                                                 ttCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCatat                                                      <  1:343257/200‑1 (MQ=255)                                                                                                                  tCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCa                                                                                                       >  4:820227/1‑150 (MQ=255)                                                                                                                  tCGTGATCCAGATAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCACAGCAATTACAAAACATACCATCACCATGAATGTTTCCGATTTCTCTCTACCGGGAGGCCCCATGaa                                                                                                                         >  3:23202/1‑132 (MQ=255)                                                                                                                     tGATCCAGTTAAAGTAAATGCATTGACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCt                                                                                                    <  4:168792/150‑1 (MQ=255)                                                                                                                        tCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACAt                                                                                                          <  2:388941/141‑1 (MQ=255)                                                                                                                         ccAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCAcc                                                                                                <  4:580598/150‑1 (MQ=255)                                                                                                                           aGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTg                                            <  1:597957/200‑1 (MQ=255)                                                                                                                            gTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGTAGGCCCTATGAATCGCTGGTAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTCCGTACTTTTTTTCATATGATTCTGtttc                                           >  1:69949/1‑198 (MQ=255)                                                                                                                              tAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCAACATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTgcgc                                         <  1:445332/200‑1 (MQ=255)                                                                                                                                aaGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGaaa                                                                                         >  4:376410/1‑150 (MQ=255)                                                                                                                                  gTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCCGCTCACCCACGaaacc                                                                                       >  4:203144/1‑148 (MQ=255)                                                                                                                                  gTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAAc                                                                                       <  4:700141/150‑1 (MQ=255)                                                                                                                                   tAAATGCATTTACCTGCTACTGTTTAGTAAGAATGTTACTAAGATCCCCAGCAATTACACAATCTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGAGAAAACAtgc                                                                                                        <  3:427700/132‑3 (MQ=255)                                                                                                                                        gCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTc                                                                                                   >  3:96477/1‑132 (MQ=255)                                                                                                                                        gCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGaa                                                                                          >  2:506343/1‑141 (MQ=255)                                                                                                                                              aCCTGCTACTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGa                                                                                    >  2:885463/1‑141 (MQ=255)                                                                                                                                                     aCTTTTTAGTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTgcgc                                                                             <  2:643097/141‑1 (MQ=255)                                                                                                                                                             gTAAAAATTTTACTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCAACGAAAACCGACTTccg                                                                              >  3:323638/1‑132 (MQ=255)                                                                                                                                                                     tttACTAAAATCCCCAGCACTTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTc  <  1:818732/200‑1 (MQ=255)                                                                                                                                                                         cTAAAATCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCa                                                         >  2:537957/1‑141 (MQ=255)                                                                                                                                                                              |                                                                                                                                                                                               CTGCCGCTGTTAGTCTTCGTTCCCAGCAAATTAAAACTGCGCGGCACGACCCGTTAAATCCCCTTACACACTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGCATTTACCTGCTACTTTTTAGTAAAAATTTTACTAAACTCCCCAGCAATTACACAAACTACCATCACCATGAATGGTTCCGATTTCTCTCTACCGGGAGGCCCTATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTACTTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTGTTTC  >  NC_000913/3222393‑3222756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.