Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,667	G→T	S84I (AGC→ATC)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,966,667	0	G→T	100.0%	336.3	89	S84I (AGC→ATC)	rho	transcription termination factor
Reads supporting (aligned to +/- strand):  new base (54/33):  ref base (1/1):  total (55/34)

AAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACG  >  NC_000913/3966473‑3966862                                                                                                                                                                                                   |                                                                                                                                                                                                    aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCG‑AGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAAt                                                                                                                                                                                               >  2:458298/1‑200 (MQ=255) aaaTATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGC‑‑AGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATc                                                                                                                                                                                              <  2:46583/200‑1 (MQ=255)   atatGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATc                                                                                                                                                                                              >  2:274333/1‑200 (MQ=255)        gggCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATacgccg                                                                                                                                                                                         >  2:599463/1‑200 (MQ=255)                   ccTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTcc                                                                                                                                                                              >  2:243515/1‑200 (MQ=255)                              tGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGAt                                                                                                                                                                   >  2:585978/1‑200 (MQ=255)                                 gTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                <  2:124014/200‑1 (MQ=255)                                   aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAt                                                                                                                                                              >  2:681747/1‑200 (MQ=255)                                      cAGGACATTATTTTGGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtctc                                                                                                                                                           <  2:566238/200‑1 (MQ=255)                                        ggACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTg                                                                                                                                                         <  2:152761/200‑1 (MQ=255)                                               attTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAt                                                                                                                                                  >  2:213129/1‑200 (MQ=255)                                                tttttGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt                                                                                                                                                 <  2:577942/200‑1 (MQ=255)                                                     gCCATCCTGAAGCATCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATc                                                                                                                                                                                              <  3:122041/150‑1 (MQ=255)                                                        aTCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgcc                                                                                                                                         >  2:198932/1‑200 (MQ=255)                                                        aTCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGATGATACCATCTCTGGTAAGATTcgcccgcc                                                                                                                                         >  2:69213/1‑200 (MQ=255)                                                         tCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATcc                                                                                                                                                                                                   >  4:207220/1‑141 (MQ=255)                                                         tCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCccgccg                                                                                                                                        >  2:446117/1‑200 (MQ=255)                                                         tCCTAAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGACGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCccgccg                                                                                                                                        >  2:539198/1‑200 (MQ=255)                                                                      cGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATc                                                                                                                                                                                              >  4:294862/1‑133 (MQ=255)                                                                       gCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATc                                                                                                                                                                                              <  1:294855/132‑1 (MQ=255)                                                                            gagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCt                                                                                                                                                                                >  4:254166/1‑141 (MQ=255)                                                                            gagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTgg                                                                                                                                                                       >  3:222190/1‑150 (MQ=255)                                                                            gagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTgg                                                                                                                                                                       >  3:487637/1‑150 (MQ=255)                                                                            gagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                <  2:487665/157‑1 (MQ=255)                                                                             agTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAttt                                                                                                                    <  2:526732/200‑1 (MQ=255)                                                                                  cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCAc                                                                                                                                                                          <  4:42111/141‑1 (MQ=255)                                                                                  cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                                                               <  2:748761/200‑1 (MQ=255)                                                                                  cGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTA‑CTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                <  3:251097/150‑1 (MQ=255)                                                                                   gAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                >  3:506329/1‑150 (MQ=255)                                                                                   gAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                <  3:192296/150‑1 (MQ=255)                                                                                      gATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtc                                                                                                                                                             <  3:393056/150‑1 (MQ=255)                                                                                      gATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                                                           >  2:71102/1‑200 (MQ=255)                                                                                        tatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCACCCTCCGCACTGGTGa                                                                                                                                                                    >  4:223568/1‑141 (MQ=255)                                                                                          tCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCa                                                                                                                                                                           >  1:286585/1‑132 (MQ=255)                                                                                          tCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTg                                                                                                                                                         >  3:251479/1‑150 (MQ=255)                                                                                           cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCAc                                                                                                                                                                          <  1:409910/132‑1 (MQ=255)                                                                                            tttGGTGATTGCTTTCGTGAAATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACt                                                                                                                                                                         <  1:67492/132‑1 (MQ=255)                                                                                                  gATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGa                                                                                               <  2:251482/200‑1 (MQ=255)                                                                                                        gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAg                                                                                                                                                    >  4:147271/1‑141 (MQ=255)                                                                                                        gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGCTTCAACCTCCGCACTGGTGATACCAtc                                                                                                                                                             >  1:105768/1‑132 (MQ=255)                                                                                                          aCTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGTCATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtctc                                                                                                                                                           >  1:246616/1‑132 (MQ=255)                                                                                                          aCTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCAtctc                                                                                                                                                           <  1:168986/132‑1 (MQ=255)                                                                                                          aCTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAt                                                                                                                                                  <  4:277153/141‑1 (MQ=255)                                                                                                          aCTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACt                                                                                       >  2:285638/1‑200 (MQ=255)                                                                                                           cTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt                                                                                      >  2:622017/1‑200 (MQ=255)                                                                                                               agaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACGGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAc                                                                                  >  2:571672/1‑200 (MQ=255)                                                                                                                gaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaaga                                                                                                                                   >  3:96570/1‑150 (MQ=255)                                                                                                                 atatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAg                                                                                                                                                    >  1:211825/1‑132 (MQ=255)                                                                                                                 atatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAg                                                                                                                                                    >  1:290418/1‑132 (MQ=255)                                                                                                                         ggatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaa                                                                        >  2:761048/1‑200 (MQ=255)                                                                                                                         ggatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCCCTGGTAAGATTCGCCCGCCGAatga                                                                                                                                   >  4:217890/1‑141 (MQ=255)                                                                                                                              gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc                                                                   >  2:102681/1‑200 (MQ=255)                                                                                                                              gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCAATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTg                                                                                                                              >  4:123623/1‑141 (MQ=255)                                                                                                                                  tGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgc                                                                                                                 <  3:585945/150‑1 (MQ=255)                                                                                                                                   ggTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGc                                                                                                                         >  4:264354/1‑141 (MQ=255)                                                                                                                                   ggTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaa                                                              >  2:146890/1‑200 (MQ=255)                                                                                                                                    gTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                                                               <  3:663722/150‑1 (MQ=255)                                                                                                                                        cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTTGCCCGCCGAAAGAAGTTGAACGCTAttt                                                                                                                    >  4:23756/1‑141 (MQ=255)                                                                                                                                        cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGACAGAAGGTGAACGCTATTTTGCgctgct                                                                                                           >  3:19149/1‑150 (MQ=255)                                                                                                                                        cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa                                                         >  2:240056/1‑200 (MQ=255)                                                                                                                                             nttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTCTTTTGCGCTGCTGAAAg                                                                                                      >  3:732518/2‑150 (MQ=255)                                                                                                                                             gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                                                               <  4:359554/141‑1 (MQ=255)                                                                                                                                             gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctc                                                    >  2:269008/1‑200 (MQ=255)                                                                                                                                             ctcccGCAGATAGCTCCTACCTCCCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCCCTGGCGATACCATCTCTGGTAACATTCACCCGCCGCAAGACGGTAAACACTCTTTTACGCTGCTGAAAg                                                                                                      >  3:350569/4‑150 (MQ=255)                                                                                                                                                       cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                                                                     <  4:361671/141‑1 (MQ=255)                                                                                                                                                       cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGt                                                                                            <  3:190186/150‑1 (MQ=255)                                                                                                                                                             cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGa                                                                                               <  4:222590/141‑1 (MQ=255)                                                                                                                                                             cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAccc                                    <  2:146954/200‑1 (MQ=255)                                                                                                                                                             cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAccc                                    >  2:745866/1‑200 (MQ=255)                                                                                                                                                              tACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                                                                     >  3:571178/1‑150 (MQ=255)                                                                                                                                                                        gTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                                                                             <  1:84923/132‑1 (MQ=255)                                                                                                                                                                        gTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                         >  2:340723/1‑200 (MQ=255)                                                                                                                                                                        gTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                         >  2:653958/1‑200 (MQ=255)                                                                                                                                                                        gTCCTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGCTAACGAAg                                                                                             >  1:402802/1‑132 (MQ=255)                                                                                                                                                                          ccTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAACGAAGGTGAACGCTATTTTCCGCTGCTCAAAGTTAACGAAGTTAACTTCTACAAACCTGaa                                                                         >  3:751252/1‑150 (MQ=255)                                                                                                                                                                          ccTGATGACATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaa                                                                         >  3:468993/1‑150 (MQ=255)                                                                                                                                                                             gatgaCATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAAc                                                                      <  3:547205/150‑1 (MQ=255)                                                                                                                                                                                  cATCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAACCCTGAAAACGCCCg                                                                 >  3:679798/1‑150 (MQ=255)                                                                                                                                                                                   aTCTACGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaa                                                                         >  4:167158/1‑141 (MQ=255)                                                                                                                                                                                       aCGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGAAAAACCTGAAAACg                                                                     >  4:252522/1‑141 (MQ=255)                                                                                                                                                                                       aCGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGACAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                                                                     >  4:406310/1‑141 (MQ=255)                                                                                                                                                                                        cGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                                                                             >  1:95834/1‑132 (MQ=255)                                                                                                                                                                                        cGTTTCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGAAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGACTGCGTa         >  2:373633/1‑200 (MQ=255)                                                                                                                                                                                         gTTTCCCCTAGCCAAATCCGCCGTGTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtttcagcagcgc                                                                            <  1:351959/132‑11 (MQ=255)                                                                                                                                                                                           ttCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg      <  2:537739/200‑1 (MQ=255)                                                                                                                                                                                            tCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATc                                                       <  3:40697/150‑1 (MQ=255)                                                                                                                                                                                            tCCCCTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa     <  2:113036/200‑1 (MQ=255)                                                                                                                                                                                              cccTATCCAAATCCGCCGTTTCAACCTCCGAACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAAc   <  2:716455/200‑1 (MQ=255)                                                                                                                                                                                               ccTATCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg  <  2:586319/200‑1 (MQ=255)                                                                                                                                                                                                   |                                                                                                                                                                                                    AAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACG  >  NC_000913/3966473‑3966862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.