Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,380	A→C	intergenic (‑13/‑122)	rne ← / → yceQ	endoribonuclease; RNA‑binding protein;RNA degradosome binding protein/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,380	0	A	C	100.0%	85.3 / NA	27	intergenic (‑13/‑122)	rne/yceQ	endoribonuclease; RNA‑binding protein;RNA degradosome binding protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/13);  total (14/13)

CAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGAAATATTTCCATCTACCGGTAAGGACTGCAACCCGCAGCCCGCTAACTGCCTGAAAGATCAATACGTCTTACGCCATTGC  >  NC_000913/1144086‑1144654                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                   aaatacaTCTTTAATTGTGGGACGACGCTTGTCACTTTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTc                                                                                                                                                                                                                                                                              <  2:139204/297‑1 (MQ=255)         tttcatggTTGGGAAGCACAGGGGCAGTGTAGTTAGCAGGGAAATATTCGCGGGCAATCTGTTTTAGTGAGAGGAAACCGTTACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGCCTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTg                                                                                                                                                                                                                                                                      <  2:19937/294‑1 (MQ=255)                gTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTATAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTa                                                                                                                                                                                                                                                               >  1:315868/1‑301 (MQ=255)                                                ggAAAGATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCggg                                                                                                                                                                                                                                  <  1:300275/298‑1 (MQ=255)                                                   aaTATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGaa                                                                                                                                                                                                                            <  1:160234/301‑1 (MQ=255)                                                          gcgGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGAGCAGTAATCCACAAAAGCAGCGTCCAGACTCGGTTCAATGCGGGTGATTTTACCTGTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATg                                                                                                                                                                                                                      <  2:12916/300‑1 (MQ=255)                                                                 aTTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGA‑TTTACCTTTGTAGGTGTTTGGCCTTTTCCGCCCGGGCCCCGGACCTTTGGAT‑TCCCGGGCATACAGAAGCTGCCCAATCACAAGGGCAACGGGGAACTTTTCCTGCCGAGGCGCCGTGGATAACAATCTTTTCAAT‑GGAACTTACTCCTTATTATTACATTGAAGACTAAGCTGCGGGCCAAATAACACCTTTCCGGGGGGGAAACGATGGCCTccggt                                                                                                                                                                                                             >  1:22595/1‑297 (MQ=255)                                                                          atttggaggaAACCGTGACTTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCCATGCGGGTTATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCAATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAgtcgc                                                                                                                                                                                                     <  1:356672/297‑1 (MQ=255)                                                                           gTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAgtcgcg                                                                                                                                                                                                    >  1:397335/1‑301 (MQ=255)                                                                             gggAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCCGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAgttgctt                                                                                                                                                                                                   >  1:181804/1‑298 (MQ=255)                                                                             gggAGGAAACCGTGACGTTCACCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGACTTGCGTTGATTACCATTCTTTTCATC‑GTAACTTACTCCTATTTCTTACTTTGACGCATCACTTGCGGGCAAATGAACTCTTTTCCGTGTGTTAGCCGATCGCCTCTTGTCtgttcgtgc                                                                                                                                                                                                   >  2:74464/1‑292 (MQ=255)                                                                                      ccGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCt                                                                                                                                                                                          >  1:371613/1‑300 (MQ=255)                                                                                                                           ccAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGATCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTATCATC‑GTAACTTACTCCTTATTCTAACATTGGCGACTAAGCTGCGGGCAAAGGAACGCCTTTCCGGGTGAGAGCCGATGGCCTAGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCgg                                                                                                                                                    <  1:74465/301‑1 (MQ=255)                                                                                                                                         atacGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCa                                                                                                                                      <  2:181796/298‑1 (MQ=255)                                                                                                                                                                  tGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GCAACTTACTCCTTATTCTTACATTGACGACTAATCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCCAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTAGGTTGCCCGATTTTCATGCGGAAATACAGCGCAATTATCaa                                                                                                             >  1:411364/1‑301 (MQ=255)                                                                                                                                                                      tcactgtttCTGTTTGAGCCCTGGA‑CTTTCGATATTCAGGTCATACATGCCACGCCCATCTACAAGGGCAAAACGCAACTCTTCCTGCTGAGTTTCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTTTTCTTACATTGACGAGTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGaa                                                                                                         <  2:315853/295‑1 (MQ=255)                                                                                                                                                                              tCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAATGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAACCTGCGGGCAAAGTAACTCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACTGTTATCGTCATCTCAACGTGGCTCAGAGTGTCGGTTGTCCGCTCTTCATTAGGCGAAACAGCGAAATTATAAAAGAAACAGAATg                                                                                                 >  1:267670/1‑301 (MQ=255)                                                                                                                                                                                      ctcccTGTA‑CATTCGCTATCCCGGACCTACGGACGCTGCCCACATACAAGTGCAACGCGCAACTCTTCCTGCTGAGTCGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTa                                                                                          <  2:22595/298‑1 (MQ=255)                                                                                                                                                                                          cTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGCGGGTCGGTTGCCCGTTTTTCCTGCGGAAAACCAGCGCAATTATCAAAGAAACCGACTGGGGATTActct                                                                                      >  2:364983/1‑300 (MQ=255)                                                                                                                                                                                                         tCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCTGGCAAAGTAACGCCTTTCCGGGTGTCAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCAAGTTTATCGTTAGATCAAAGAGGCGCCGAGTGTCGGTTCCCCGTTTTTCATTCGGCAAAACAGCGCAATTATCCAAGCAATAAACTGAGTTTTACTCTCCAGAACTCTTTca                                                                        >  2:244504/1‑299 (MQ=255)                                                                                                                                                                                                                      cgcgttGCCCTTGTAGATGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGAAATATTTCCATCTACCGGTAAgg                                                          >  1:110032/6‑301 (MQ=255)                                                                                                                                                                                                                           tGCCCTTGTAGATGGGCAACGCGCCACTATTCCTTCTGATTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACTTTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGAAATATTTCCATCTACCGGTAAGGACTGc                                                     <  2:110028/301‑1 (MQ=255)                                                                                                                                                                                                                                 tctaccaacgccaCGCTCAACTCTTTCTGCTGCGTTTGGTTGATTAACATTCTTTTCATC‑GTAACTTACGCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGAAATATTTCCATCTACCGGTAAGGACTGCAACCCg                                               <  2:115614/289‑1 (MQ=255)                                                                                                                                                                                                                                                     cgtgtccttctgcattgaggttcttAACATTCTTCTGATCTGCTACTT‑CTCCTTATTCTTACATTGACTACTAAGCTGCGGGCAACGTAACGCCTTTCCGGGTGTGAACCGATGGCCGCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATTCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGAAATATTTCCATCTACCGGTAAGGACTGCAACCCGCAGCCCGCTAACTGCCTGaa                           <  2:345560/278‑1 (MQ=255)                                                                                                                                                                                                                                                            ggaagAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCACACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTTCCCAGAAATATTTCCATCTACCGGTAAGGACTGCAACCCgagcacggaagagcacacgtctgaact                     >  1:115618/5‑274 (MQ=255)                                                                                                                                                                                                                                                                              aaCATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGAAATATTTCCATCTACCGGTAAGGACTGCAACCCGCAGCCCGCTAACTGCCTGAAAGATCAATACGTCTTACGCCATtg   >  1:317414/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aaCATTCTTTTCATC‑GTAACTTACTCCTTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTAGCGTCGCCAACCTCCCGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGTTTGCCCGTCTTTCATGCGGAAAAACAGTCCTATTATCAATGAAACATACTGGGTATTACTTCCTACAAAGATTTCTATCTACCGGTACGGCCTGCCACCCGCAGCCCGCTACCTGTCTGCAAACTCAATACGACTTTCGCCCTtgt  >  2:43093/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                   CAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGCGGGCAATTTCTTTTAGTGGGAGGAAACCGTGACGTTCAGCGCCGTAATCAACAAAAGCAGCTTCCAGACTCGGTTCAATGCGGGTGATTTTACCTTTGTAGATGTTTGCCTTTTTCTGCTCGTGCCCTGGA‑CTTTCGATATCCAGGTCATACAGACGCTGCCCATCTACAAGGGCAACGCGCAACTCTTCCTGCTGAGTTGCGTTGATTAACATTCTTTTCATC‑GTAACTTACTCATTATTCTTACATTGACGACTAAGCTGCGGGCAAAGTAACGCCTTTCCGGGTGTGAACCGATGGCCTCGTGTCTAGTCGCGTCGCCAACCTCACGGTTATCGTCAGCTCAAAGAGGCGCAGAGTGTCGGTTGCCCGTTTTTCATGCGGAAAAACAGCGCAATTATCAAAGAAACAGACTGGGTATTACTCTCCAGAAATATTTCCATCTACCGGTAAGGACTGCAACCCGCAGCCCGCTAACTGCCTGAAAGATCAATACGTCTTACGCCATTGC  >  NC_000913/1144086‑1144654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑