Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,117,034 T→A E63D (GAA→GAT)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,117,0340TA97.9% 139.4 / ‑6.7 48E63D (GAA→GAT) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (25/22);  minor base G (0/1);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGAC  >  NC_000913/4116740‑4117324
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                  
cAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTAc                                                                                                                                                                                                                                                                                               >  1:53390/1‑300 (MQ=255)
                     gccgcgGGAGCCTTCCACATGGACGAGGATCCACTGCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGGCCGGTATTTATGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGc                                                                                                                                                                                                                                                                          <  2:359670/298‑1 (MQ=255)
                     acgtgtgctcttccgatcttGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGc                                                                                                                                                                                                                                                                          <  1:363547/281‑1 (MQ=255)
                              gCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGCCGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTACCAGAGAATAGTGGTCTCACGCTGGTTCGTACTACCGATAGCTGCAATCTGACCGGAACTGATATCGGCTTTCCCCATCCCACCCACCACGGTGGAGCCTTGGTTGcccacacttgc                                                                                                                                                                                                                                                                 >  2:248028/1‑292 (MQ=255)
                               ccTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCTAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCTGGTTTCTTTTTCCCAGCCAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATTGGCTTTCGCTAGCACATCTACCCGCGTGGAGTTTTGGTTGGACCAGATTTcaa                                                                                                                                                                                                                                                               >  2:213940/1‑299 (MQ=255)
                                    cacaTGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg                                                                                                                                                                                                                                                          >  1:316506/1‑301 (MQ=255)
                                    cacaTGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTCCTAAACCGTCACGTTTTAAATGCTCGCAGATCTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGCCAATCGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTTGGTGGCCCAGATCTCCATTggg                                                                                                                                                                                                                                                          >  1:78835/1‑301 (MQ=255)
                                        tGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCCGCGTGGAGCGTGtggtgggagatcggcagagggtcgtgg                                                                                                                                                                                                                                                      >  2:363529/1‑280 (MQ=255)
                                          gTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGATTTCACGCTGGTTCGTAATACCGATTGCTGCAATTTGATCGGAACTGATATCGGCTTTCTCCATCACATCTACCAGCGTCGAGCTTTTGGTGGCCCAGATTTCCATTGGGTCgtgt                                                                                                                                                                                                                                                    >  1:202403/1‑301 (MQ=255)
                                               ggATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCCGACAATAGTGGTTTCACGCTGGTTCGTAAAACCGATAGCTGCAATTTGCTCGGAATTGATATCGGCTTTCGCCAGCACAACTCACAGCGTGGGGATTTGGGTGGCCCTGATTTCTATTTGGTCGTGttttc                                                                                                                                                                                                                                                >  2:289440/1‑297 (MQ=255)
                                                             ttGGAGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAAGCTTCTAAACCGTCACGTTTGAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTg                                                                                                                                                                                                                                 <  1:289454/301‑1 (MQ=255)
                                                               ggggacAGGAAAGTACGGGGCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTAACGTTGTAAATGCTCGAAGAATTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCATTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTgg                                                                                                                                                                                                                                <  2:202395/295‑1 (MQ=255)
                                                                gTGCCAGAAAAGTACGGGTCAATAACCAAACCGGTATTGTTGCGTATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGAGTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTggg                                                                                                                                                                                                                               <  2:401897/300‑1 (MQ=255)
                                                                      gAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGCTTCTTGTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAGTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTTGGTGGCCCAGAGTTCTATTGGGTCGTGTTCTACCCAACATTGTTTGGGGTATAttt                                                                                                                                                                                                                        >  2:196008/1‑301 (MQ=255)
                                                                                 ttCAATGTCCTGACCGGTATTGCTGCCTATATACGTTTCCTACACTTGTCTTTTTAAATGCTCGCAGAGTTCTGGGGTACGACGGCACTGCCAGACCAGGGCGTTTTAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTTGTTCGTAATACCGATAGCTGCAATTTGATCGGAGCTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTcg                                                                                                                                                                                                             <  2:298451/300‑1 (MQ=255)
                                                                                               cGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCATTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCt                                                                                                                                                                                               <  1:213949/301‑1 (MQ=255)
                                                                                                            gATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATCTTGGCa                                                                                                                                                                                  >  1:188595/1‑301 (MQ=255)
                                                                                                                       ctAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTTGGTCGTGTTCTACCCAACCTGGTTGTGGGTAGATTTGCCACAATTCGCGCTGTGACACGCTTATGATATTGGCATCGTAATCCAt                                                                                                                                                                       >  1:57764/1‑301 (MQ=255)
                                                                                                                         aaacagtcaatgttacaaTGGTCGCAGATTTCTGCGGTACGATGGCACTGCCAGACAATGGCGTGATAGATAGGATTGCCGGTTTCTTTTTCCCAGACAAGAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTa                                                                                                                                                                     <  2:78834/285‑1 (MQ=255)
                                                                                                                           aCCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACTATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGTACTGATATCGGCTTTTGCTAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTCGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTCATTCATTa                                                                                                                                                                     >  1:140107/1‑299 (MQ=255)
                                                                                                                                 tcagacgtgtgctcttccgATCTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTGATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                                                                                             <  1:73632/283‑1 (MQ=255)
                                                                                                                                 aCGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGTTTCGAAACGCTAATGATATTGGACTCGGGTTCCATTACGACcgcg                                                                                                                                                             >  2:206056/1‑301 (MQ=255)
                                                                                                                                  cGTTTTAAATGCTCGCAGATTTCTTCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgc                                                                                                                                                            <  2:53389/301‑1 (MQ=255)
                                                                                                                                      ttAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGa                                                                                                                                                        <  1:255581/301‑1 (MQ=255)
                                                                                                                                        aaaTGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGTGCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCCCAAATTCGCGCTGCGACACGCTAATAATATTGGCATCGTGATCCATTACGACCGCGGGGGAg                                                                                                                                                       >  1:221530/1‑300 (MQ=255)
                                                                                                                                                     tttCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCTATAGCTGCAATGTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCGTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTg                                                                                                                                         <  1:248038/301‑1 (MQ=255)
                                                                                                                                                        cTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTACTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGTTTTGGGTGGCCCAGATTTCCATTGGGTCGCGTTCTACCTAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATTATATTGGCATCGTTCACCTTTACGACtgcttgatcggaatagcgccgtttagg                                                                                                                                      >  2:73631/1‑277 (MQ=255)
                                                                                                                                                        cTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTTTTCTACCCCACCTGGTTTTGGGTTGATTTTCTCAAATTCGCTCTTCGACACGCTACTGATATTGGCATCTTGTACCATTCCGACCGCGCGGGAGCTGGTGGTGCCCTGGt                                                                                                                                       >  2:20141/1‑300 (MQ=255)
                                                                                                                                                         tggtgtacagcgGCAGTGCCATAAAATGGGGTTATAGCAAGGCTTGCCGGTTTTTTTTTCCCAGAAATGAGTGTTTTCACGCTGGGTCGTAATACCGATAGCTGCAATTTGATCGGAACTGAGATCGGCTTTCGCCAGCACATCTACCGGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAGCCTGGTGTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTc                                                                                                                                      <  2:316491/290‑1 (MQ=255)
                                                                                                                                                                 gacgGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTGCTGGGTAGATTTGCTCAAATTCGCGCTTCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCCCGGGAGCTGGTGGTGCCCTGGTGGAGCGCaa                                                                                                                              >  2:248187/1‑300 (MQ=255)
                                                                                                                                                                        cTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGTTGGTGCCCTGGTCGAGCGCAACGatatat                                                                                                                      >  1:192303/1‑301 (MQ=255)
                                                                                                                                                                          gCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAttt                                                                                                                    >  1:17208/1‑301 (MQ=255)
                                                                                                                                                                               tacaTGGGGTTATTGATAGGCTTGCCGGTTTCTTTTTCCCAGAACATATTGGTTTCACGCTGGTTCGTAATACCGAGAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttt                                                                                                                 <  2:397387/296‑1 (MQ=255)
                                                                                                                                                                                  aTGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCTCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGGAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCCGCGGAGCTGGTGGTGCACGGGTCGGACGCAACGAACGATTTTTTTTCAg                                                                                                            >  2:22579/1‑301 (MQ=255)
                                                                                                                                                                                           tcgttagGGTTGGCGGTTTCTTTTTCCCTGACAATAGTGGTTTCCCGCTGGTTCGTGATACCGATAGATGCAATTTGGTCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTa                                                                                                   <  2:17208/297‑1 (MQ=255)
                                                                                                                                                                                             gatagGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAt                                                                                                 >  1:252448/1‑301 (MQ=255)
                                                                                                                                                                                                              cTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCTACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATAtt                                                                                >  1:312424/1‑301 (MQ=255)
                                                                                                                                                                                                               tttttCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTa                                                                               <  1:248197/301‑1 (MQ=255)
                                                                                                                                                                                                                             tAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGAGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGCTATTGGCATCGTGATCCATTACGACCGCGCGGGCGCTGGGTGTGCCCTGGTCGGGCGCAACTATATATTTTTTTTCAGTCATTTTTTATTGTACCTTAGTcgcttcatagccaacgttttt                                                                 >  2:10789/1‑281 (MQ=255)
                                                                                                                                                                                                                                   gttCTCGCTGGTTCGTAATACCGATAGCTGCAATTTGAACGGAACTGATGACGGCTTTCGCCAGGACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGGTTTCCATTGGGTCGTGTGCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGGGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCt                                                            <  2:188587/299‑1 (MQ=255)
                                                                                                                                                                                                                                          ctgagtccaaatcactgtAGCTGCAATTTTGGCGGAACTGCTATCTGCTTGAGCCAGCACATCTAGCAGCGTGGAGCTTGGGGTGCACCAGATTTCAATTGGGTCGTGTGCTACCCATCCTGGTTTTGGGTAGATTTGCGCAAATTCGCGCGTCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCTCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGt                                                    <  2:221521/284‑1 (MQ=255)
                                                                                                                                                                                                                                                        cGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTGAGTCCTGGTTAATTGTCCCGTAGTCATATTACAGCGAAGATTTTTTTTCTGAAGGTGTTGTGGTTTCCtttt                                      >  2:417251/1‑301 (MQ=255)
                                                                                                                                                                                                                                                          aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGTTATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTttc                                     <  1:196016/300‑1 (MQ=255)
                                                                                                                                                                                                                                                             gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCATCATATCTACCAGCGTGGAGCTTTGGGTGGCTCAGATTTTCATTGGGTCGTGTTCCACCCAACCTGGTTTTGTGTTGATTTCCTCCAATTCCCGCTGCAACACCATAATGATATTGGCATCGTGATCCACTACTGCCACGCGCGCGCTCGTGGCTCCCGGCTCGTGCGCAACGCTATATTTTTATTCAGCCCTGTTTCATTGTCGCCTAGTCCTATCACAGCAAACTCCTGAGTTCTGAAGTGGTTGTGTTTTCCTTttctctt                                 >  2:69993/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                    ttttATTGGAACTGGTATCGGCTTTCGCCCTCACGTCTTCCAGCTTGGAGCTTTTGGTGGCCCAGATTTCCATTGTGTCGTGTTCTACCCATCCTGGTTTTGAGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAacac                          <  2:252437/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gAACTGATATCGCCTTTCTCCAGCCCATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCCAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATc                   <  1:22579/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aaCTGATATCGGCTTTCGCCAGCACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGc                 <  1:206064/301‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            tggCCAGGACATCTACCAGCGTGGAGCTTTGGGTGGCCCAGGTTTCCATTGGATCGTGTGCTACCCATCCTGATTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGGCCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGAc  <  1:284208/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                  
CAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGGATATAATCTTCTAAACCGTCACGTTTTAAATGCTCGCAGATTTCTGCGGTACGACGGCACTGCCAGACAATGGCGTTATAGATAGGCTTGCCGGTTTCTTTTTCCCAGACAATAGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTTGTGGTTTCCTTTTCTTCCACAACACAGATATCGCAAGGCAAATGGCGAC  >  NC_000913/4116740‑4117324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑