Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,188,929	C→A	R1194S (CGT→AGT)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,188,929	0	C	A	100.0%	134.3 / NA	44	R1194S (CGT→AGT)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/19);  total (25/19)

CTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGC  >  NC_000913/4188646‑4189212                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                            ctgccTGCAGGGTAAGGCGATGGTTCAGCTGCAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGGGTATTCCGAAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGCTGGAAGCACGTCGTGCGAAAGAGCCGGCAATCCTTGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAACCCAAAGGTAAACGTCGTCTGGTTATCACCCCAGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTc                                                                                                                                                                                                                                                                            <  1:24773/298‑1 (MQ=255)  cgccTGCGGGGTAAAGCGGTTGTTCAACTGGACGATGGAGGACAGATCGGTTCTGGGGCACCCCTGGGGCGTATTCCGCAGGAATCCGGCGGGACCAAGGAAATCGCCGGTGGTCTGCGCGGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCg                                                                                                                                                                                                                                                                           <  2:319894/299‑1 (MQ=255)                             cTGGAAGAGGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGa                                                                                                                                                                                                                                                  <  1:297212/299‑1 (MQ=255)                                        cGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAACTCAACGTGTTCGAAGGTGAACGTTTAGAACGTGGGGACGTAATTTccac                                                                                                                                                                                                                                      >  2:407781/1‑298 (MQ=255)                                          ttctgATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACgg                                                                                                                                                                                                                                   <  1:361352/297‑1 (MQ=255)                                              gATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTGGACGGTCGCGATCCGTACGAAGAGAGGATTCCGAAATGGAGTCCTCTCAACGTCTTCGCAGCTGAACGGGTACAACGTGGTGACGTAATTTCCCACgccgaa                                                                                                                                                                                                                               >  2:361334/1‑296 (MQ=255)                                                   tcccaggtgagacccggggcctaaTTCCGCAGGAGTCCGGCGGTACCAAGGACATCACCGTGGGTCTGCCGCGCGTTGCGTGCCAGTACGAAGAACGTCGTCCGAAAGGGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAgc                                                                                                                                                                                                                          <  2:393586/278‑1 (MQ=255)                                                    ctctGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAACGGTATCGTTTCCTTCGGTAAAGAAACCAAACGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCATACGAAGAGATGATTCCGAAATGGATTCAGCTCAACGTTTTCGAAGGTGAACGTGAAGAACGGGGTGACGTGATTTCCGTAGGTCCGGAAgtg                                                                                                                                                                                                                         >  1:344250/1‑299 (MQ=255)                                                             cacCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAACTGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTACTTTCCGACGGTCCGGAAGCGCCGAACGa                                                                                                                                                                                                                 >  2:7865/1‑300 (MQ=255)                                                             cacCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGa                                                                                                                                                                                                                 >  1:324146/1‑300 (MQ=255)                                                               cccTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCCGAAATCAGCGGTATCGTTTCCTTCGGGAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGCCGGTCGCGATCCGTACGAAAAGCTGATTCCGAAATGGACTCAGCTCAACTTTTTCTAAGGTGCACGTCGAGAACGTGGGAGAGGAAGTTCGCAAGGTCCGGACGCACCGCATGAc                                                                                                                                                                                                                >  2:422231/1‑299 (MQ=255)                                                                ttcagacgggtgctcttccgatctcggcggTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGACTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACAt                                                                                                                                                                                                              <  1:3541/276‑1 (MQ=255)                                                                     cgcgTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGCCTGGTTATCACCCTGGTAGACGGTAGCGATCCGTACGACGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATtctgcg                                                                                                                                                                                                        >  1:325398/1‑301 (MQ=255)                                                                             ccGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGAGCCGTACGACATTCTGCGTCTGCgtg                                                                                                                                                                                                >  2:349262/1‑301 (MQ=255)                                                                               gCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGCCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTCTGAAGAGATGATTCCGAAATGGAGGCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAAGTTCCGACGGTCCGGAAGcgccgagatcggaagagcacatgtctgaa                                                                                                                                                                                              >  1:152008/1‑277 (MQ=255)                                                                                atctaaTCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCgtggt                                                                                                                                                                                              <  1:155834/296‑1 (MQ=255)                                                                                  ggAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGtt                                                                                                                                                                                           >  1:315249/1‑301 (MQ=255)                                                                                    aaTCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGGACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGACGCGCCGCACGACATTCTGCGTCGGCgtggtagat                                                                                                                                                                                          >  2:155828/1‑296 (MQ=255)                                                                                        cggcggTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTCTTCGAAGGTGAACGTTTAGAACGGGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACAtcgaagggacgaacgtcgagtagggg                                                                                                                                                                                     >  2:3541/1‑276 (MQ=255)                                                                                            ggTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATGCTGCGTCTGCGTGGTGTTCATGCTGTTa                                                                                                                                                                                 >  1:308687/1‑301 (MQ=255)                                                                                              tACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTa                                                                                                                                                                                 >  1:139292/1‑299 (MQ=255)                                                                                              tACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTAc                                                                                                                                                                                <  1:349278/300‑1 (MQ=255)                                                                                              aaCCACGGACCTCACCGGTTGTCTGGCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACt                                                                                                                                                                               <  2:139287/300‑1 (MQ=255)                                                                                                                                                     cgtcCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTa                                                                                                                        >  1:193354/1‑301 (MQ=255)                                                                                                                                                               agCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAac                                                                                                               <  1:407801/300‑1 (MQ=255)                                                                                                                                                                 ccGGCAATCCTTGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAaca                                                                                                              <  1:7865/299‑1 (MQ=255)                                                                                                                                                                             aattaaaTCAGCGGGATCGTGTCAGTCGGTAAAGAAACGAAAGGTAAACGTCGTCTGGTTAGCACCCCGGAAGACCGTATCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtc                                                                                                 <  2:325383/296‑1 (MQ=255)                                                                                                                                                                                   aacgtgtgctcttccgatctCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTc                                                                                          <  1:122826/282‑1 (MQ=255)                                                                                                                                                                                         ggTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGAGATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCAACGTTAACGTTCGTAAGAtgg                                                                                    >  2:155679/1‑300 (MQ=255)                                                                                                                                                                                                    cTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACTTCGTTAACGAAGTACAGGACGTATACCTTCCGCAGTGCGTTAAGATTAACGTGAAAAACAACGAAGTTCTCGTTCGACAGATGCCTCGTAAAGc                                                                          >  2:55310/1‑300 (MQ=255)                                                                                                                                                                                                       cGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTAATCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACCAAACGAAGTTAACGTTCGGCAGAtaggaagtgcgtcggtt                                                                      >  2:122822/1‑285 (MQ=255)                                                                                                                                                                                                         atAAAGAAACAAAAGGTGAACGGCGTCTGGGTATCACCCCGGTAGAGGGTAGCGATCCGAACGAAGAGATGATTCGGAAAGGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCAt                                                                    <  2:324131/300‑1 (MQ=255)                                                                                                                                                                                                             aGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGAGGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGCTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTTCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTACGATTAACGATACACACATCGAAGTTATCGTTCGTCCGTTGCTGCGTAAAGCTCCCATCttt                                                                >  1:374747/1‑301 (MQ=255)                                                                                                                                                                                                                 aCCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACCTCTTTAACGAAGTACAGGCCGTATACCGTCTGCAGGGCGCTAAGATTAACGATACACACATCGAAGTTATCGTTCGTTCGATGCCGCGTAACGCTACCATCATTAAca                                                            >  2:34525/1‑300 (MQ=255)                                                                                                                                                                                                                 aCCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGAAGTAATTTCCGACGGTCCGGAAGAGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAAGACTTATACCCTCTGCAGGGCGTTAGTATTAACGATAAAAACATCGAAGTTATAGTTCGCAAGATGTTGTCTACAGTTATCATCTTTAAcg                                                            >  2:274300/1‑301 (MQ=255)                                                                                                                                                                                                                       ggTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTa                                                      >  1:373904/1‑301 (MQ=255)                                                                                                                                                                                                                                 gtcTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACtt                                            >  1:250995/1‑301 (MQ=255)                                                                                                                                                                                                                                       ttATAACACCGGTAGACGGTAGCGGTCCGTACGAAGATATGTTTCCGAAATGGAGTCAACTCAACGGGTTCGAAGGTGCACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGa                                      <  2:308672/301‑1 (MQ=255)                                                                                                                                                                                                                                        tATCACCCCGGTAGACGGTAGCGATCCGGACGAAGAGAGGATTCCGAAATGGAGTCAGCTCAACGGGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGAAATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGaa                                     <  2:315234/301‑1 (MQ=255)                                                                                                                                                                                                                                             ccccGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGa                                >  1:42229/1‑301 (MQ=255)                                                                                                                                                                                                                                                      aCGGTAGCGATCCGTACGAAGAGATGAATCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGAAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTg                        <  2:250984/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cgctCCGGACGAAGGGGTGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATActc                 <  2:42229/297‑1 (MQ=255)                                                                                                                                                                                                                                                                        aaGAGATGATTCCGAAATGGAGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGaa     >  1:6437/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             atgatgACGAAAGGGAGTCAGCTCAACGTGTTGGAAGGTGAACGTGTAGAACGTGGGGACGTAATTGCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGc  <  1:2531/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                            CTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGCACGTCGTCCGAAAGAGCCGGCAATCCTGGCTGAAATCAGCGGTATCGTTTCCTTCGGTAAAGAAACCAAAGGTAAACGTCGTCTGGTTATCACCCCGGTAGACGGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTGAATACTCTCGCGTCAAGATCGC  >  NC_000913/4188646‑4189212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑