Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,676	G→T	R87L (CGC→CTC)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,966,676	0	G→T	100.0%	331.6	86	R87L (CGC→CTC)	rho	transcription termination factor
Reads supporting (aligned to +/- strand):  new base (51/35):  ref base (0/0):  total (51/35)

AACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGT  >  NC_000913/3966489‑3966866                                                                                                                                                                                            |                                                                                                                                                                                               aaCCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCt                                                                                                                                                                                    >  2:298816/1‑200 (MQ=255)       gCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCAc                                                                                                                                                                              >  2:555598/1‑200 (MQ=255)              tGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGAt                                                                                                                                                                       >  2:467295/1‑200 (MQ=255)                   aaGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCAt                                                                                                                                                                  >  2:640369/1‑200 (MQ=255)                        ggACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTg                                                                                                                                                             >  2:358898/1‑200 (MQ=255)                             ttattTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAg                                                                                                                                                        >  2:495860/1‑200 (MQ=255)                                tttttGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt                                                                                                                                                     <  2:499405/200‑1 (MQ=255)                                         tCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATcctcc                                                                                                                                                                                              >  4:9740/1‑150 (MQ=255)                                                      cGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAAc                                                                                                                               >  2:491933/1‑200 (MQ=255)                                                      cGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAAc                                                                                                                               >  2:583709/1‑200 (MQ=255)                                                            gagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTgg                                                                                                                                                                           >  4:375175/1‑150 (MQ=255)                                                            gagTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAtt                                                                                                                         >  2:511878/1‑200 (MQ=255)                                                                ggCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgc                                                                                                                     <  2:295842/200‑1 (MQ=255)                                                                  agAAGATATCTTGGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCAc                                                                                                                                                                              <  1:469895/140‑1 (MQ=255)                                                                   gAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                    <  4:467238/150‑1 (MQ=255)                                                                   gAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                    <  4:406519/150‑1 (MQ=255)                                                                        tatCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCAtctc                                                                                                                                                               >  4:591242/1‑150 (MQ=255)                                                                         atCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGc                                                                                                                                                                                >  3:65651/1‑132 (MQ=255)                                                                         atCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaa                                                                                                            >  2:244969/1‑200 (MQ=255)                                                                         atCTTTGGTGATGGCGTACTGGAGATATTGCAGGACGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGAGGACATCGACGTTTCACCTAGCCAAATCATCCGGTTCAACCTCCGc                                                                                                                                                                                >  3:382548/1‑132 (MQ=255)                                                                           cTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCAc                                                                                                                                                                              >  3:510202/1‑132 (MQ=255)                                                                           cTTTGGTGATGGCGTACTGGAGATATTACAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATAc                                                                                                                                                                     >  1:1737/1‑141 (MQ=255)                                                                               ggTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTaa                                                                                                      >  2:20772/1‑200 (MQ=255)                                                                                  gATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGa                                                                                                   >  2:2279/1‑200 (MQ=255)                                                                                  gATGGCGTACTGAAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAAACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGAtt                                                                                                                                                     <  4:568379/150‑1 (MQ=255)                                                                                     ggCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                    <  3:550966/132‑1 (MQ=255)                                                                                     ggCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                    <  3:422528/132‑1 (MQ=255)                                                                                     ggCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                    >  3:386729/1‑132 (MQ=255)                                                                                        gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGttt                                                                                                                                                                                          <  1:383094/107‑1 (MQ=255)                                                                                        gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGttt                                                                                                                                                                                          >  3:383074/1‑107 (MQ=255)                                                                                        gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCAtc                                                                                                                                                                 >  3:311484/1‑132 (MQ=255)                                                                                        gTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGATCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCCCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                                                                         >  2:637914/1‑188 (MQ=255)                                                                                          aCTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgcccgcc                                                                                                                                             >  4:118401/1‑150 (MQ=255)                                                                                             ggAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcg                                                                                                                                                   >  1:160224/1‑141 (MQ=255)                                                                                               agaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTa                                                                                                                                                          <  3:473763/132‑1 (MQ=255)                                                                                                gaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTcgccc                                                                                                                                                >  1:516441/1‑141 (MQ=255)                                                                                                 atatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGCTAAGATTcgcccg                                                                                                                                               >  1:243310/1‑141 (MQ=255)                                                                                                    ttGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCccgccg                                                                                                                                            >  1:85560/1‑141 (MQ=255)                                                                                                    ttGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                                                                                 >  2:374450/1‑200 (MQ=255)                                                                                                            tggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                                                                         <  2:673821/200‑1 (MQ=255)                                                                                                             ggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCccgccg                                                                                                                                            >  3:485013/1‑132 (MQ=255)                                                                                                             ggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAt                                                                                                                          <  4:674635/150‑1 (MQ=255)                                                                                                              gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGTTGATACCATCTCCGGTAAGATTCGCCCGCCGAACGAAGGTGAACGCTAtt                                                                                                                         >  4:116357/1‑150 (MQ=255)                                                                                                                   ggTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAaagaa                                                                                                                                      >  3:342132/1‑132 (MQ=255)                                                                                                                   ggTTTCCTCCGTTCCGCAGACAGCGCCTACCTCGCCGTTCCTGATGGCATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTAGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcg                                                                                                                    <  4:392777/150‑1 (MQ=255)                                                                                                                         ctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAAt                                                            <  2:624055/200‑1 (MQ=255)                                                                                                                             gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCt                                                                                                                            >  3:38494/1‑132 (MQ=255)                                                                                                                             gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTgcgc                                                                                                                   >  1:246978/1‑141 (MQ=255)                                                                                                                             gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCCCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCt                                                                                                                            >  3:275144/1‑132 (MQ=255)                                                                                                                              ttCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                                                                         <  4:637848/150‑1 (MQ=255)                                                                                                                               tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                                                                        >  4:497194/1‑150 (MQ=255)                                                                                                                                 cGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTAttt                                                                                                                        <  3:17031/132‑1 (MQ=255)                                                                                                                                     gACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGaa                                                                                                  >  4:570449/1‑150 (MQ=255)                                                                                                                                      aCAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAg                                                                                                          <  1:281496/141‑1 (MQ=255)                                                                                                                                       cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                                                                         <  1:480580/141‑1 (MQ=255)                                                                                                                                        aGCTCCTACCTCGCCGGTCCGGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGtt                                                                                               <  4:224435/150‑1 (MQ=255)                                                                                                                                         gCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa                                                                                                       >  1:125207/1‑141 (MQ=255)                                                                                                                                          cTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                                                               >  3:273570/1‑132 (MQ=255)                                                                                                                                           tCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAc                                          <  2:534920/200‑1 (MQ=255)                                                                                                                                             cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt                                                                                          <  4:298787/150‑1 (MQ=255)                                                                                                                                             cTACCGCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAccc                                        <  2:563788/200‑1 (MQ=255)                                                                                                                                                ccTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                                                                         <  3:173237/132‑1 (MQ=255)                                                                                                                                                ccTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGc                                     <  2:9741/200‑1 (MQ=255)                                                                                                                                                      cGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt                                                                                          <  1:342152/141‑1 (MQ=255)                                                                                                                                                        gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                               >  4:337530/1‑150 (MQ=255)                                                                                                                                                        gTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGCTAACGAAg                                                                                                 >  3:524324/1‑132 (MQ=255)                                                                                                                                                          ccTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAAcc                                                                                                                                                                                     <  2:252020/46‑1 (MQ=255)                                                                                                                                                          ccTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAAcc                                                                                                                                                                                     >  4:251993/1‑46 (MQ=255)                                                                                                                                                          ccTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaa                                                                             >  4:431260/1‑150 (MQ=255)                                                                                                                                                          ccTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaaa                           >  2:691537/1‑200 (MQ=255)                                                                                                                                                           cTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                    >  1:114004/1‑62 (MQ=255)                                                                                                                                                           cTGATGACATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATAcc                                                                                                                                                                    <  3:113994/62‑1 (MQ=255)                                                                                                                                                                  cttCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCCGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTc                   <  2:188587/198‑1 (MQ=255)                                                                                                                                                                  cATCTACGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGa                                                                              >  1:82468/1‑141 (MQ=255)                                                                                                                                                                       aCGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaaca                                                                >  4:399803/1‑150 (MQ=255)                                                                                                                                                                       aCGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt              >  2:633669/1‑200 (MQ=255)                                                                                                                                                                        cGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                                                                                 >  4:685456/1‑132 (MQ=255)                                                                                                                                                                        cGTTTCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                                                                                 <  2:685531/132‑1 (MQ=255)                                                                                                                                                                            tCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa         <  2:375225/200‑1 (MQ=255)                                                                                                                                                                            tCCCCTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa         <  2:587876/200‑1 (MQ=255)                                                                                                                                                                             ccccTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaa                                                                            <  3:373930/132‑1 (MQ=255)                                                                                                                                                                              cccTAGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAAc       <  2:399854/200‑1 (MQ=255)                                                                                                                                                                                  aGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc                                                                       <  3:125197/132‑1 (MQ=255)                                                                                                                                                                                  aGCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCttt                                                     >  4:531659/1‑150 (MQ=255)                                                                                                                                                                                   gCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa                                                             >  1:335217/1‑141 (MQ=255)                                                                                                                                                                                   gCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaaa                                                             >  1:202925/1‑141 (MQ=255)                                                                                                                                                                                   gCCAAATCCTCCGTTTCAACCTCCGCACTGGTGATACCATCTCAGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt  <  2:106554/200‑1 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                               AACCTGGCTCGTATGCGTAAGCAGGACATTATTTTTGCCATCCTGAAGCAGCACGCAAAGAGTGGCGAAGATATCTTTGGTGATGGCGTACTGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGT  >  NC_000913/3966489‑3966866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.