Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,051,535	G→A	A2200T (GCC→ACC)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,051,535	0	G→A	100.0%	271.6	69	A2200T (GCC→ACC)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand):  new base (56/13):  ref base (0/0):  total (56/13)

ACGCTGATCCCTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGAACGCCGGCGAGGCT  >  NC_000913/2051352‑2051729                                                                                                                                                                                        |                                                                                                                                                                                                   aCGCTGATCCCTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAAcggc                                                                                                                                                                                    >  1:384051/1‑200 (MQ=255)             gTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCATGAAAAACGGCGGCTGATTATGa                                                                                                                                                                        >  1:214871/1‑200 (MQ=255)                       gtgtTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGc                                                                                                                                                              >  1:65357/1‑200 (MQ=255)                           tcatcaAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTCCAAGCTCTg                                                                                                                                                          >  1:799533/1‑200 (MQ=255)                                aaGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGcctcc                                                                                                                                                     <  1:737672/200‑1 (MQ=255)                                     ggTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTAcc                                                                                                                                                                                                  >  2:394644/1‑150 (MQ=255)                                     ggTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGt                                                                                                                                                >  1:593075/1‑200 (MQ=255)                                          gAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATg                                                                                                                                           >  1:278119/1‑200 (MQ=255)                                               ccACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTCTTATCAGTTGAATAATGACAACTTTACACCAGGaaaaa                                                                                                                                                                                        >  2:184320/1‑150 (MQ=255)                                               ccACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTAccc                                                                                                                                                                                                 <  4:72625/141‑1 (MQ=255)                                               ccACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTAATATCAGTTGGATAATGACAACTTTAccc                                                                                                                                                                                                 >  4:172962/1‑141 (MQ=255)                                                         atTCACTCGCGCAGAAGAAAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGAACTACCGACAACTACATGCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTAcccc                                                                                                                                                                                                <  3:122723/132‑1 (MQ=255)                                                            cACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAg                                                                                                                         <  1:278743/200‑1 (MQ=255)                                                                                    gAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGctc                                                                                                                                                            >  4:82365/1‑141 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGACTAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACAGGTAAAGTGACATTTAAACATGTCGTCAGCAAttag                                                                                            >  1:567438/1‑198 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAACACGGCGGCTGATTATGAGTTTTCAAGCTCTGcct                                                                                                                                                       >  4:585992/1‑141 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCaa                                                                                                                                                                >  3:160613/1‑132 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCaa                                                                                                                                                                >  3:593910/1‑132 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGcct                                                                                                                                                       >  4:520478/1‑141 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCg                                                                                                                                              >  2:188172/1‑150 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCg                                                                                                                                              >  2:573083/1‑150 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCg                                                                                            >  1:392478/1‑200 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCg                                                                                            >  1:165730/1‑200 (MQ=255)                                                                                         gTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTACGAGTTTTCAAGCTCTGCCTCCTGGGTCg                                                                                                                                              >  2:784433/1‑150 (MQ=255)                                                                                              gTATCAGTCAATGGTACTGACCTACCGACAACTCCATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAACGACAACTTTACCCCAGGAAAAACGGCGGATGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGtt                                                                                                                                         >  2:626895/1‑150 (MQ=255)                                                                                              gTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGtt                                                                                                                                         >  2:87000/1‑150 (MQ=255)                                                                                                  cAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCg                                                                                                                                              <  4:188250/141‑1 (MQ=255)                                                                                                  cAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGAtt                                                                                   >  1:274958/1‑200 (MQ=255)                                                                                                      cAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACgg                                                                               >  1:281874/1‑200 (MQ=255)                                                                                                         tGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGt                                                                                                                                                <  3:418222/132‑1 (MQ=255)                                                                                                          ggTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGAc                                                                           >  1:311720/1‑200 (MQ=255)                                                                                                           gTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCg                                                                                                                                              >  3:87235/1‑132 (MQ=255)                                                                                                           gTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATg                                                                                                                                     >  4:87044/1‑141 (MQ=255)                                                                                                           gTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATg                                                                                                                                     >  4:380896/1‑141 (MQ=255)                                                                                                                gACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTAcc                                                                                                                                >  4:501592/1‑141 (MQ=255)                                                                                                                          cAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGa                                                                                                                      >  4:557440/1‑141 (MQ=255)                                                                                                                          cAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGa                                                                                                                      >  4:42025/1‑141 (MQ=255)                                                                                                                          cAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGc                                                           >  1:681802/1‑200 (MQ=255)                                                                                                                            aCTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGt                                                                                                                             >  3:634313/1‑132 (MQ=255)                                                                                                                             cTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCTGGGGTCGATGTTGATGCTACCGGTa                                                                                                                            >  3:565247/1‑132 (MQ=255)                                                                                                                             cTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACAt                                                                                                                   >  4:332934/1‑141 (MQ=255)                                                                                                                             cTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCt                                                        >  1:400614/1‑200 (MQ=255)                                                                                                                                   tCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTaaaa                                                                                                             <  4:617696/141‑1 (MQ=255)                                                                                                                                    cccTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGgcag                                                                                                   >  2:809737/1‑150 (MQ=255)                                                                                                                                     ccTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGt                                                >  1:674407/1‑200 (MQ=255)                                                                                                                                      cTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTCTGAGATTTCAAGCTCTGCCTCCTGGGTCGAGGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTAGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCTAGAGCACCTAGATATGta                                               >  1:560582/1‑200 (MQ=255)                                                                                                                                        tCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGtata                                             >  1:668427/1‑200 (MQ=255)                                                                                                                                            aGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTaaaa                                                                                                             <  3:635332/132‑1 (MQ=255)                                                                                                                                                gTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAg                                                                                       >  2:666840/1‑150 (MQ=255)                                                                                                                                                gTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCCAAATCAGGAGGCCCTAGCTATGTATACGAAATct                                     >  1:388404/1‑199 (MQ=255)                                                                                                                                                  tCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCgt                                   >  1:85184/1‑200 (MQ=255)                                                                                                                                                          gCGTATTATCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAagag                           >  1:720913/1‑200 (MQ=255)                                                                                                                                                              attatCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTa                                                                                  >  4:389913/1‑141 (MQ=255)                                                                                                                                                                 atCAGTTGAATAATGACAACTTTACCACAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACgg                                                                               <  4:93498/141‑1 (MQ=255)                                                                                                                                                                  tCAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTggtggg                   <  1:299859/200‑1 (MQ=255)                                                                                                                                                                   cAGTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCg                                                                             >  4:310376/1‑141 (MQ=255)                                                                                                                                                                     gTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGa                >  1:404401/1‑200 (MQ=255)                                                                                                                                                                     gTTGAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGa                >  1:272389/1‑200 (MQ=255)                                                                                                                                                                        gAATAATGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGAttcc                                                                                 >  3:507884/1‑130 (MQ=255)                                                                                                                                                                         aataatGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGTAATTCGGAAAGGATTACGGCGACGCCAAAATCagga                                                              >  2:813417/1‑150 (MQ=255)                                                                                                                                                                         aataatGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACg                                                                                <  3:501562/132‑1 (MQ=255)                                                                                                                                                                         aataatGACAACTTTACCCCAGGAAAAACGGCGGCTGATTACGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGAACGc            <  1:784512/200‑1 (MQ=255)                                                                                                                                                                          ataatGACAACTGTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACgg                                                                               <  3:591863/132‑1 (MQ=255)                                                                                                                                                                            aatGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGc                                                           >  2:484043/1‑150 (MQ=255)                                                                                                                                                                            aatGACAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGAACGCCgg         >  1:147745/1‑200 (MQ=255)                                                                                                                                                                                 cAACTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCagg                                                               >  4:568025/1‑141 (MQ=255)                                                                                                                                                                                   aCTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCaa                                                                      >  3:9388/1‑132 (MQ=255)                                                                                                                                                                                   aCTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGAACGCCGGCGAGGCt  >  1:29637/1‑200 (MQ=255)                                                                                                                                                                                   aCTTTACCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGCGGGTGAACGCCGGCGAGGCt  >  1:237434/1‑200 (MQ=255)                                                                                                                                                                                        |                                                                                                                                                                                                   ACGCTGATCCCTGTATTGAATGGTGTTCATCAAGCTGGTCTGAGTACCACAATACAATTCACTCGCGCAGAAGACAAAATAATGAGCGGTACAGTATCAGTCAATGGTACTGACCTACCGACAACTACATTCCCTTCGCAGGGGTTCACCGGGGCGTATTATCAGTTGAATAATGACAACTTTGCCCCAGGAAAAACGGCGGCTGATTATGAGTTTTCAAGCTCTGCCTCCTGGGTCGATGTTGATGCTACCGGTAAAGTGACATTTAAAAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGAACGCCGGCGAGGCT  >  NC_000913/2051352‑2051729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.