Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,857,648	G→T	E186* (GAA→TAA)	mutS →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,857,648	0	G→T	100.0%	283.2	74	E186* (GAA→TAA)	mutS	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  new base (37/36):  ref base (0/1):  total (37/37)

TGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGAC  >  NC_000913/2857463‑2857844                                                                                                                                                                                          |                                                                                                                                                                                                     tGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAAtt                                                                                                                                                                                        <  1:492430/200‑1 (MQ=255)  gTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCGGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTg                                                                                                                                                                                       >  1:723568/1‑200 (MQ=255)   ttGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGa                                                                                                                                                                                      >  1:101994/1‑200 (MQ=255)   ttGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGa                                                                                                                                                                                      >  1:277093/1‑200 (MQ=255)     nnnnnngCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAANNNNNNCGGCAGANNNNNNNCGCACTANNNNNNNGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAg                                                                                                                                                                                    <  1:517496/194‑1 (MQ=255)       aGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGAACGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGc                                                                                                                                                                                  <  1:68033/200‑1 (MQ=255)                                 gcTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCt                                                                                                                                                        >  1:759924/1‑200 (MQ=255)                                                aGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTCAATGTCGTTa                                                                                                                                                                                           >  2:713172/1‑150 (MQ=255)                                                 gCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCCACGCACTACTCCTGCGGAACTGCGGTATGCAGAAGATTTTGCTTAAATGTCGTTaa                                                                                                                                                                                          >  2:64932/1‑150 (MQ=255)                                                     aGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTc                                                                                                                                                                                               >  4:541826/1‑141 (MQ=255)                                                      ggTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGacac                                                                                                                                   <  1:569086/200‑1 (MQ=255)                                                      ggTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGacac                                                                                                                                   <  1:817245/200‑1 (MQ=255)                                                      ggTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACCGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGacac                                                                                                                                   <  1:674192/200‑1 (MQ=255)                                                                   cgACGCTGGATATCAGTTCCGGGCGTTTTCGCCGGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGcc                                                                                                                                                                        <  2:109153/150‑1 (MQ=255)                                                                    gACGCTGGATATCAGTACCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACGAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAGATGTCGTTAAt                                                                                                                                                                                         <  3:403321/132‑1 (MQ=255)                                                                     aCGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTAGCGGCCTg                                                                                                                                                                      >  2:564532/1‑150 (MQ=255)                                                                        cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTgcgc                                                                                                                                                                   <  2:314921/150‑1 (MQ=255)                                                                        cTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGaa                                                                                                                 >  1:245908/1‑200 (MQ=255)                                                                          ggATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATc                                                                                                               >  1:513305/1‑200 (MQ=255)                                                                           gATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCAGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCt                                                                                                              <  1:502921/200‑1 (MQ=255)                                                                             tatCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCt                                                                                                                                                                       >  4:190012/1‑141 (MQ=255)                                                                                 aGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTgcgc                                                                                                                                                                   >  4:590436/1‑141 (MQ=255)                                                                                  gTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTACATGTCGTTAATTGAAGGCCGTcgcg                                                                                                                                                                           >  3:81148/1‑132 (MQ=255)                                                                                  gTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGACGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGCATCTGCAAttt                                                                                                       >  1:262001/1‑200 (MQ=255)                                                                                  gTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGc                                                                                                                                                         <  2:111125/150‑1 (MQ=255)                                                                                     ccGGGCGCTTTCGCCGGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGGACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGcc                                                                                                                                                                        <  3:209303/132‑1 (MQ=255)                                                                                       gggCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGAc                                                                                                  <  1:176661/200‑1 (MQ=255)                                                                                        ggCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCTTTTGCCGTCGCCCGCTGTggg                                                                                                                                                   <  2:172735/150‑1 (MQ=255)                                                                                        ggCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTggg                                                                                                                                                   <  2:643147/150‑1 (MQ=255)                                                                                          cGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAg                                                                                                                                                 <  2:395665/150‑1 (MQ=255)                                                                                          cGTTTGCGCCTGGGCGAACCAGCTGACCGCGAAACGATGGCTGCAGAACTGCAACGCACTAATCCTGCGGAGCTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTGATTGAAGGCCGTCGCGGCCTgcgc                                                                                                                                                                   <  3:437365/132‑1 (MQ=255)                                                                                           gTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGCTTTTGCTTAAATGTCGTTAATTGAAGACCGTCGCGGCCTGCGcc                                                                                                                                                                  >  3:439080/1‑132 (MQ=255)                                                                                           gTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGcc                                                                                                                                                                  <  3:425122/132‑1 (MQ=255)                                                                                           gTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCcgc                                                                                              >  1:70651/1‑200 (MQ=255)                                                                                           gTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGACCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCcgc                                                                                              >  1:118209/1‑200 (MQ=255)                                                                                                cGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCt                                                                                         >  1:200709/1‑200 (MQ=255)                                                                                                   cTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAg                                                                                                                                                 <  4:339548/141‑1 (MQ=255)                                                                                                   cTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAg                                                                                                                                                 <  4:357995/141‑1 (MQ=255)                                                                                                     gAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGtt                                                                                                                                               >  4:539010/1‑141 (MQ=255)                                                                                                     gAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGa                                                                                                                                      >  2:449376/1‑150 (MQ=255)                                                                                                        cGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGacac                                                                                                                                   <  2:395615/150‑1 (MQ=255)                                                                                                            ccgggtggacgggAAACGAAGGCGGCAGAACTGCAACGCGATAAGCCTGCGGAACGGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAg                                                                                                                                                 <  3:597733/120‑1 (MQ=255)                                                                                                            ccGTCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAg                                                                                                                                                 <  3:418410/132‑1 (MQ=255)                                                                                                            ccGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAg                                                                                                                                                 >  3:548645/1‑132 (MQ=255)                                                                                                            ccGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAg                                                                                                                                                 <  3:266619/132‑1 (MQ=255)                                                                                                            ccGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAg                                                                                                                                                 <  3:23766/132‑1 (MQ=255)                                                                                                              ggCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACcgcgcg                                                                                                                             >  2:555639/1‑150 (MQ=255)                                                                                                                cTGACCGGGAAACGATGGCGGCAGAACTGAAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGcc                                                                                                                           <  2:572363/150‑1 (MQ=255)                                                                                                                 tGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGacac                                                                                                                                   <  4:42904/141‑1 (MQ=255)                                                                                                                          aaCGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGacac                                                                                                                                   <  3:335270/132‑1 (MQ=255)                                                                                                                               tGGCGGCAGAACTGCAACGCACTAAACCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGt                                                                                                                     <  4:199398/141‑1 (MQ=255)                                                                                                                                            gCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAAtt                                                                                                        >  4:255867/1‑141 (MQ=255)                                                                                                                                                  cACTAATCCTGCGGAACTGCTGTATGCAGAAGTTTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGACATCGACACCGCGCGCCAGCAGTTGATTCTGCAATTTGGGACCCGCGATcc                                                                                         >  2:196888/1‑149 (MQ=255)                                                                                                                                                  cACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGCTGAATCTGCa                                                                                                           >  3:605004/1‑132 (MQ=255)                                                                                                                                                  cACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCACCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGtt                                       >  1:697471/1‑200 (MQ=255)                                                                                                                                                    cTAATCCTGCGGAACTGCTGTATGCAGACGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCTGTTGAATCTGCAATTTGGGAccc                                                                                                >  4:54533/1‑141 (MQ=255)                                                                                                                                                        tCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGt                                 <  1:456908/200‑1 (MQ=255)                                                                                                                                                          cTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATg                                                                                                                                                                                                 >  1:277919/1‑38 (MQ=255)                                                                                                                                                          cTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATg                                                                                                                                                                                                 <  2:277896/38‑1 (MQ=255)                                                                                                                                                          cTGCGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTg                               >  1:202951/1‑200 (MQ=255)                                                                                                                                                             cGGAACTGCTGTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAg                            <  1:705571/200‑1 (MQ=255)                                                                                                                                                                  ctgctgTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTc                                                                         >  2:28834/1‑150 (MQ=255)                                                                                                                                                                    gctgTATGCAGAAGATTTTGCTTATACGCCGCTAATTGAACGCCGTCGCGGCCTGACCCGTCGCTCTCTGTTGGTGTTTGAAATCGACACTCTGCGCCACCAGTTGAATCTGCAATATGGGACCCGCGAtat                                                                                         >  3:469961/1‑130 (MQ=255)                                                                                                                                                                    gctgTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCTCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCTATTTGGGACCCGCGATCTGGTCGGtgt                                                                                >  4:340127/1‑139 (MQ=255)                                                                                                                                                                    gctgTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGttt                                                                                <  4:433925/141‑1 (MQ=255)                                                                                                                                                                    gctgTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAg                            >  1:179501/1‑193 (MQ=255)                                                                                                                                                                     ctgTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGaa                    <  1:89573/200‑1 (MQ=255)                                                                                                                                                                     ctgTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGaa                    <  1:673370/200‑1 (MQ=255)                                                                                                                                                                       gTATGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTgg                                                                             >  4:232255/1‑141 (MQ=255)                                                                                                                                                                         aTGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCg                                                                                    >  3:230403/1‑132 (MQ=255)                                                                                                                                                                         aTGCAGAAGATTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGAt                >  1:621452/1‑200 (MQ=255)                                                                                                                                                                             agaagaTTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATAccc            <  1:592709/200‑1 (MQ=255)                                                                                                                                                                                agaTTTTGCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAAc         >  1:478504/1‑200 (MQ=255)                                                                                                                                                                                       gCTTAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGAc  <  1:689844/200‑1 (MQ=255)                                                                                                                                                                                          |                                                                                                                                                                                                     TGTTGCAGGAGCGTCAGGACAACCTGCTGGCGGCTATCTGGCAGGACAGCAAAGGTTTCGGCTACGCGACGCTGGATATCAGTTCCGGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTATGCGAAAGATACCCAACGTACGAC  >  NC_000913/2857463‑2857844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.