Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,539	T→C	intergenic (‑121/‑18)	queE ← / → yqcG	7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis/membrane stress resistance protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	2,905,539	0	T→C	100.0%	227.0	59	intergenic (‑121/‑18)	queE/yqcG	7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis/membrane stress resistance protein
Reads supporting (aligned to +/- strand):  new base (21/38):  ref base (0/0):  total (21/38)

CCGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATAC  >  NC_000913/2905360‑2905726                                                                                                                                                                                    |                                                                                                                                                                                            ccGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         <  1:437790/200‑1 (MQ=255) ccGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGAGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTTCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         <  1:442194/200‑1 (MQ=255)  cGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGt                                                                                                                                                                        <  1:332418/200‑1 (MQ=255)                            gTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACg                                                                                                                                                                                <  1:625647/166‑1 (MQ=255)                            gTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         >  1:658226/1‑173 (MQ=255)                                             aagcGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAAGCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGt                                                                                                                                                                               <  2:60800/147‑1 (MQ=255)                                             aaTCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCAACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGt                                                                                                                                                                               <  2:711645/150‑1 (MQ=255)                                                   gTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         <  2:658160/150‑1 (MQ=255)                                                     aCTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACg                                                                                                                                                                                <  4:492996/141‑1 (MQ=255)                                                      cTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         <  1:349093/147‑1 (MQ=255)                                                      cTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         >  2:349064/1‑147 (MQ=255)                                                      cTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGt                                                                                                                                                                        <  1:381754/148‑1 (MQ=255)                                                      cTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGt                                                                                                                                                                        >  2:381713/1‑148 (MQ=255)                                                       tGCATAGCATTCTCTGGGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAggg                                                                                                                                                                                       <  3:276694/132‑1 (MQ=255)                                                        gCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtatta                                                                                                                                                                           >  1:374905/1‑143 (MQ=255)                                                        gCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtatta                                                                                                                                                                           <  2:374864/143‑1 (MQ=255)                                                        gCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtat                                                                                                                                                                             <  1:641238/141‑1 (MQ=255)                                                        gCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtat                                                                                                                                                                             >  2:641173/1‑141 (MQ=255)                                                          aTAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtat                                                                                                                                                                             <  1:713323/139‑1 (MQ=255)                                                          aTAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtat                                                                                                                                                                             >  2:713256/1‑139 (MQ=255)                                                          aTAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATAATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCagaag                                                                                                                                                                  <  2:799183/150‑1 (MQ=255)                                                          aTAGCATTCTCTGTGAAGTGGATAATTGTTAATAATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTGTAAAGAAAAATGCGAACCATATCGGTACTAAGCACAGGGAGt                                                                                                                                                                                    >  3:541008/1‑132 (MQ=255)                                                             gCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGt                                                                                                                                                                        <  4:264118/141‑1 (MQ=255)                                                             gCATACTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAAc                                                                                                                                                                                 <  3:348129/132‑1 (MQ=255)                                                              cATTCTGTGGGAAGTGGATAATTGTTAATTATTGCAGAGCCTGCCACAACAATCATGTCTTATTAACATCCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTc                                                                                                                                                                       <  4:122888/141‑1 (MQ=255)                                                               aTTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCTACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAAt                                                                                                                                                             <  2:771749/150‑1 (MQ=255)                                                               aTTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGt                                                                                                                                                                               <  3:335581/132‑1 (MQ=255)                                                                 tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATaa                                                                                                                                                           >  2:602449/1‑150 (MQ=255)                                                                 tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATaa                                                                                                                                                           >  2:554676/1‑150 (MQ=255)                                                                 tctcTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGa                                                                                                                                                 <  1:602509/160‑1 (MQ=255)                                                                    ctGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         >  4:463165/1‑133 (MQ=255)                                                                     tgtgAAGTGGATAATTGTTAATTATTGCAGGTCATGCCAAAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         <  3:463144/132‑1 (MQ=255)                                                                     gggggaGTGGATAATGGTTAATGATTGCAGGTCATGCCACAACAAGCATGGCTTATTAACGTTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         <  3:611270/127‑1 (MQ=255)                                                                       tgAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTc                                                                                                                                                                       <  3:138104/132‑1 (MQ=255)                                                                             ggATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtat                                                                                                                                                                             <  4:186695/120‑1 (MQ=255)                                                                             ggATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtat                                                                                                                                                                             >  3:186689/1‑120 (MQ=255)                                                                             ggATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGAtt                                                                                                                                               >  2:400574/1‑150 (MQ=255)                                                                                   ttGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAggg                                                                                                                                                                                       <  3:114919/104‑1 (MQ=255)                                                                                   ttGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAggg                                                                                                                                                                                       >  4:114923/1‑104 (MQ=255)                                                                                   ttGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATgg                                                                                                                                                  >  4:227721/1‑141 (MQ=255)                                                                                          ttattGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGt                                                                                                                                                                               >  4:461081/1‑105 (MQ=255)                                                                                          ttattGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGt                                                                                                                                                                               <  3:461060/105‑1 (MQ=255)                                                                                             ttGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAGAGACAAATTCGAAACATATCGTTTATAATCAAAGTGAGTAACGTATTATg                                                                                                                                                                         >  3:474796/1‑108 (MQ=255)                                                                                             ttGCAGAGCCTGCAACAACAATCATATCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         <  4:474818/108‑1 (MQ=255)                                                                                              tGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGAt                                                                                                                                                <  3:227711/132‑1 (MQ=255)                                                                                                        gCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAAt                                                                                                                    <  2:392676/150‑1 (MQ=255)                                                                                                                 aTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAggg                                                                                                                                                                                       >  3:477458/1‑74 (MQ=255)                                                                                                                 aTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAggg                                                                                                                                                                                       <  4:477480/74‑1 (MQ=255)                                                                                                                 aTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         <  2:714753/88‑1 (MQ=255)                                                                                                                 aTCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATg                                                                                                                                                                         >  1:714820/1‑88 (MQ=255)                                                                                                                        cTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAggg                                                                                                                                                                                       >  2:163719/1‑67 (MQ=255)                                                                                                                        cTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAggg                                                                                                                                                                                       <  1:163731/67‑1 (MQ=255)                                                                                                                        cTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGt                                                                                                                                                                               >  2:658198/1‑75 (MQ=255)                                                                                                                        cTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGt                                                                                                                                                                               <  1:658264/75‑1 (MQ=255)                                                                                                                                                ggTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtatta                                                                                                                                                                           >  4:156787/1‑55 (MQ=255)                                                                                                                                                ggTTTAAAGAAAAATGCGAAACATATCGTTAATAATCAAAGGGAGTAACGtatta                                                                                                                                                                           <  3:156782/55‑1 (MQ=255)                                                                                                                                                               gCGAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATaaa                                                                      <  4:279078/141‑1 (MQ=255)                                                                                                                                                                 gAAACATATCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAAt                                                                    <  4:629345/141‑1 (MQ=255)                                                                                                                                                                        atCGTTAATAATCAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATAc  <  1:400622/200‑1 (MQ=255)                                                                                                                                                                                    |                                                                                                                                                                                            CCGGTAAAGTAACCCTCACCTTGCAGGGTCTGGAACATCTCGTTAATCGGGTACTGCATAGCATTCTCTGTGAAGTGGATAATTGTTAATTATTGCAGATCCTGCCACAACAATCATGTCTTATTAACATTCTGTTACAGGCAGGTTTAAAGAAAAATGCGAAACATATCGTTAATAATTAAAGGGAGTAACGTATTATGTCAGAAGAAAATAAAGAAAATGGATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATAC  >  NC_000913/2905360‑2905726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.