Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,229,827	A→G	N314D (AAC→GAC)	ygjK →	alpha‑glucosidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,229,827	0	A→G	100.0%	281.7	77	N314D (AAC→GAC)	ygjK	alpha‑glucosidase
Reads supporting (aligned to +/- strand):  new base (41/35):  ref base (0/0):  total (42/35)

CTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATCCGGACATCGCCAAAGAGAATATCCGCGCGGTCTTCTCC  >  NC_000913/3229637‑3230003                                                                                                                                                                                               |                                                                                                                                                                                 cTATTCCCATCTGCGGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACt                                                                                                                                                                         <  1:536518/200‑1 (MQ=255)          tCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGGTCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCAc                                                                                                                                                                >  1:455231/1‑200 (MQ=255)                     cTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGgcggtgcg                                                                                                                                                     >  1:494324/1‑200 (MQ=255)                                      gagCAAATGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGt                                                                                                                                    >  1:690554/1‑200 (MQ=255)                                           aaTGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  1:702687/200‑1 (MQ=255)                                           aaTGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  1:604548/200‑1 (MQ=255)                                            aTGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGAc                                                                                                                                                                                >  2:553290/1‑150 (MQ=255)                                                   tCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACt                                                                                                                                                                         <  2:587119/150‑1 (MQ=255)                                                     cgcgATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCTATCCGGATGCGACGCCAGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGAc                                                                                                                     <  1:717659/200‑1 (MQ=255)                                                         atatTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCggg                                                                                                                 >  1:353542/1‑200 (MQ=255)                                                           atTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAATGCTGGGAAGAATACCTGAAGACAGGGTTAACCAATCCGGCTACGCCGCCGGAACCGTCGCGCATCCCGTTGAAAGCCATTGAAACGCTCGACGGCAACTgtacctca                                                                                                                                                                 >  2:532416/1‑143 (MQ=255)                                                           atTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGCTGCGACGCCGGAACAGACGCGCGTCGCGGTGCAAGCGAGCGAAACGCTCGACGGCAAc                                                                                                                                                                          >  4:270545/1‑141 (MQ=255)                                                           atTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATAACTGAAGAAAGGGTTAACCAATCCGGATGCGAAGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGATCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACAACGTGACACCGGCGGTGCACGGgcg                                                                                                               >  1:713734/1‑200 (MQ=255)                                                           atTCTGGCGCGTCCGGCGTTTCATCTCACCGCCTCCCAGCAACGCTGGGAAGAATACCTGAAGAAAGCGTTTACCAATCCGGATGCGCCGCCGGAACAGACCCGCGTCGCGGTGAAAGCGCTCGACACGCCCGACGGTAACTGGCGCCCa                                                                                                                                                                 >  2:44669/1‑150 (MQ=255)                                                                ggCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGACGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGt                                                                                                          <  1:498374/200‑1 (MQ=255)                                                                   gcgTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGgcgg                                                                                                                                                         <  2:287090/150‑1 (MQ=255)                                                                               ttATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGgcggtgc                                                                                                                                                      >  4:427358/1‑141 (MQ=255)                                                                               ttATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGgcggtgc                                                                                                                                                      >  4:163891/1‑141 (MQ=255)                                                                                tATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAAttt                                                                                                                                            <  2:455180/150‑1 (MQ=255)                                                                                    tcACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGa                                                                                                                                                 <  4:635313/141‑1 (MQ=255)                                                                                      aCCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAGGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGaaa                                                                                                                                               <  4:579443/141‑1 (MQ=255)                                                                                          ccTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTgg                                                                                >  1:58701/1‑200 (MQ=255)                                                                                          ccTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGGGAAATTTAACACCGTTa                                                                                                                                  >  2:288345/1‑150 (MQ=255)                                                                                             cGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGa                                                                                                                                                 <  3:215079/132‑1 (MQ=255)                                                                                             cGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAAGCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  2:549554/150‑1 (MQ=255)                                                                                                    aCGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGa                                                                      >  1:489565/1‑200 (MQ=255)                                                                                                    aCGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGa                                                                      >  1:149527/1‑200 (MQ=255)                                                                                                      gccgggAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  4:2424/138‑1 (MQ=255)                                                                                                      gCTGGGAAGAATACCTGAAGAAATGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGGGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  4:339399/141‑1 (MQ=255)                                                                                                      gCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               >  4:252598/1‑141 (MQ=255)                                                                                                      gCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  4:312210/141‑1 (MQ=255)                                                                                                      gCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  4:515686/141‑1 (MQ=255)                                                                                                       cTGGGAAGAATACCTGAAGGAAGGGTTAACCAAACCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGAc                                                                                                                     <  2:31337/150‑1 (MQ=255)                                                                                                         gggAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCg                                                                                                                                     >  3:623081/1‑132 (MQ=255)                                                                                                         gggAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCaa                                                                 >  1:580892/1‑199 (MQ=255)                                                                                                         gggAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAg                                                                 >  1:213858/1‑200 (MQ=255)                                                                                                           gaagaaTACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGtt                                                                                                                                   >  3:615143/1‑132 (MQ=255)                                                                                                              gaaTACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGgcgc                                                                                                              >  2:776605/1‑150 (MQ=255)                                                                                                               aaTACCTGAAGAAAGGGTTAACCAATGCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  3:73150/132‑1 (MQ=255)                                                                                                               aaTACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  3:374310/132‑1 (MQ=255)                                                                                                               aaTACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTacac                                                                                                                               <  3:148291/132‑1 (MQ=255)                                                                                                                aTACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGAc                                                                                                                     <  4:410139/141‑1 (MQ=255)                                                                                                                 tACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTgg                                                                                                           >  2:289942/1‑150 (MQ=255)                                                                                                                 tACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGc                                                         >  1:782862/1‑200 (MQ=255)                                                                                                                   ccTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAGAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGGTACAACGTc                                                                                                                           >  3:455505/1‑132 (MQ=255)                                                                                                                      gaagaaAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTtctc                                                                                                      >  2:308985/1‑150 (MQ=255)                                                                                                                      gaagaaAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTtctc                                                                                                      >  2:347915/1‑150 (MQ=255)                                                                                                                      gaagaaAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATgg                                                    >  1:488635/1‑200 (MQ=255)                                                                                                                       aagaaAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTg                                                                                                                       >  3:564575/1‑132 (MQ=255)                                                                                                                       aagaaAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTcc                                                                                                     >  2:442236/1‑150 (MQ=255)                                                                                                                           aaGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCAt                                               <  1:64390/200‑1 (MQ=255)                                                                                                                             gggTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAATGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGAc                                                                                                                     >  1:516272/1‑128 (MQ=255)                                                                                                                             gggTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAAGTGGCGCTCACCGGGCGGGGCGGTGAAATTTAACACCGTTACACCGGCGGTGACCGGGCGCTGGTTCGCCGGCAAt                                                                                               >  2:709613/1‑150 (MQ=255)                                                                                                                             gggTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGAc                                                                                                                     <  2:516218/128‑1 (MQ=255)                                                                                                                             gggTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTtc                                                                                                        >  4:285071/1‑141 (MQ=255)                                                                                                                             gggTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCAttt                                             >  1:588712/1‑200 (MQ=255)                                                                                                                             gggTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCCCACCGGGCGGCGCGGTGATATTGAACACCGTTCCACCGGCGGCTACCgag                                                                                                                 >  3:518268/1‑130 (MQ=255)                                                                                                                             gggTTAACCAATCCGGATGCCACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCCCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCCGGTTCTCAGGCaac                                                                                               >  2:36574/1‑149 (MQ=255)                                                                                                                                  aaCCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATc                                        >  1:469258/1‑200 (MQ=255)                                                                                                                                  aaCCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCCACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAACTTTAACACCGTTACACCGTCGGTGACCGGGCGCTg                                                                                                            >  3:33820/1‑132 (MQ=255)                                                                                                                                       aTCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATCCGGAc                                   >  1:22738/1‑200 (MQ=255)                                                                                                                                       aTCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGCGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATc                                                                                              >  4:222429/1‑141 (MQ=255)                                                                                                                                        tCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGcc                                                                                    <  2:474999/150‑1 (MQ=255)                                                                                                                                           ggATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGAGCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGAc                                                                                          >  4:438788/1‑141 (MQ=255)                                                                                                                                                 ggcGCCGGAACAGACGGGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGcc                                                                                    <  4:270893/139‑1 (MQ=255)                                                                                                                                                 gACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATAGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGcc                                                                                    <  4:40790/141‑1 (MQ=255)                                                                                                                                                   cGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATCCGGACATCGCCAAagag                       <  1:487728/200‑1 (MQ=255)                                                                                                                                                   cGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATCCGGACATCGCCAAagag                       >  1:364598/1‑200 (MQ=255)                                                                                                                                                    gCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATCCGGACATCGCCAAAgaga                      <  1:291773/200‑1 (MQ=255)                                                                                                                                                     ccGGAACAGACGCGCGTCGCGGTGAAGGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGAcc                                                                                         <  3:285060/132‑1 (MQ=255)                                                                                                                                                     ccGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATCCGGACATCGCCAAAGAGaa                     <  1:694552/200‑1 (MQ=255)                                                                                                                                                     ccGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATCCGGACATCGCCAAAGAGaa                     >  1:692605/1‑200 (MQ=255)                                                                                                                                                        gAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAg                                                                    <  2:68304/150‑1 (MQ=255)                                                                                                                                                                  gcgTCGCGGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGc                                                                   <  4:615175/141‑1 (MQ=255)                                                                                                                                                                        cgGTGAAAGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATCCGGACATCGCCAAAGAGAATATCCGCGCGGTCTTCTcc  <  1:116158/200‑1 (MQ=255)                                                                                                                                                                              aaGCGATCGAAACGCTCGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGa                                                       <  4:155081/141‑1 (MQ=255)                                                                                                                                                                                   atggAAGCGCTGGACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTTTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGgcg                                                  <  4:40719/138‑1 (MQ=255)                                                                                                                                                                                               |                                                                                                                                                                                 CTATTCCCATCTGCTGACCGCTCAGGAAGTTAGCAAAGAGCAAATGCAGATCCGCGATATTCTGGCGCGTCCGGCGTTTTATCTCACCGCCTCGCAGCAACGCTGGGAAGAATACCTGAAGAAAGGGTTAACCAATCCGGATGCGACGCCGGAACAGACGCGCGTCGCGGTGAAAGCGATCGAAACGCTCAACGGTAACTGGCGCTCACCGGGCGGTGCGGTGAAATTTAACACCGTTACACCGTCGGTGACCGGGCGCTGGTTCTCCGGCAATCAGACCTGGCCGTGGGATACCTGGAAGCAGGCGTTTGCGATGGCGCATTTCAATCCGGACATCGCCAAAGAGAATATCCGCGCGGTCTTCTCC  >  NC_000913/3229637‑3230003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.