Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,597,488	A→G	W652R (TGG→CGG)	opgB ←	OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	4,597,488	0	A→G	100.0%	336.7	96	W652R (TGG→CGG)	opgB	OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane‑bound) and II (soluble)
Reads supporting (aligned to +/- strand):  new base (55/39):  ref base (0/0):  total (56/40)

CTTCATTGCCCAGCACAAGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAAC  >  NC_000913/4597314‑4597683                                                                                                                                                                               |                                                                                                                                                                                                    cTTCATTGCCCAGCACAAGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCCGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAATTCCCGCTa                                                                                                                                                                            >  1:338897/1‑200 (MQ=255) cTTCATTGCCCAGCACAAGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTa                                                                                                                                                                            >  1:578966/1‑200 (MQ=255)   tCATTGCCCAGCACAAGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTaaa                                                                                                                                                                          >  1:414857/1‑200 (MQ=255)   tCATTGCCCAGCACAAGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTaaa                                                                                                                                                                          >  1:278765/1‑200 (MQ=255)             gCACAAGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCACATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTAAcc                                                                                                                                                                >  1:45532/1‑200 (MQ=255)             gCACAAGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCAcc                                                                                                                                                                >  1:804182/1‑200 (MQ=255)             gCACAAGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCAcc                                                                                                                                                                <  1:199442/200‑1 (MQ=255)                 aaGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCtctt                                                                                                                                                            >  1:243722/1‑200 (MQ=255)                   gagTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCtcttct                                                                                                                                                          >  1:27572/1‑200 (MQ=255)                            cttcATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCg                                                                                                                                                 >  1:770840/1‑200 (MQ=255)                                   gCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGt                                                                                                                                          >  1:609935/1‑200 (MQ=255)                                   gCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGt                                                                                                                                          >  1:754438/1‑200 (MQ=255)                                            tACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGa                                                                                                                                 >  1:212024/1‑200 (MQ=255)                                              ccGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCATCGGCCCCGTGACTCCGGAc                                                                                                                                                                                          >  4:176042/1‑141 (MQ=255)                                              ccGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCgaaa                                                                                                                                                                                          >  4:207729/1‑138 (MQ=255)                                              ccGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATcc                                                                                                                                                                                 >  2:481062/1‑150 (MQ=255)                                                ggAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACgg                                                                                                                                                                                        >  4:224186/1‑141 (MQ=255)                                                ggAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACt                                                                                                                             >  1:544878/1‑200 (MQ=255)                                                 gAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTg                                                                                                                            >  1:696830/1‑200 (MQ=255)                                                 gAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTCGTACTCGATTTTTACTTCATCGCCCAGCTTCCCGTTGGACCAGCGGCCCCTCGACt                                                                                                                                                                                                >  3:425097/1‑132 (MQ=255)                                                           gCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGTAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGt                                                                                                                  >  1:234425/1‑200 (MQ=255)                                                               gCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCAcc                                                                                                                                                                <  2:74905/150‑1 (MQ=255)                                                                cGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGa                                                                                                             >  1:647221/1‑200 (MQ=255)                                                                  ttATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAat                                                                                                           >  1:77597/1‑200 (MQ=255)                                                                    aTTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATGTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAatat                                                                                                         <  1:338635/200‑1 (MQ=255)                                                                       gCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCAc                                                                                                                                                                 >  4:565039/1‑141 (MQ=255)                                                                       gCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTgg                                                                                                                                                        >  2:368919/1‑150 (MQ=255)                                                                       gCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCAAGCTGAGCGTTGGACAAGCGTCCCCGCGACTCCGGACGGGAAACCCCGCTaca                                                                                                                                                                          >  3:237223/1‑130 (MQ=255)                                                                         cGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACct                                                                                                                                                               <  4:398532/141‑1 (MQ=255)                                                                            aTGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCCTGTACTCGATTTTTACTTCATCGTCCAGCTGCGCGTTGGACCAGTCGCCCCGCGACCCCTGACGGCTAATCCCGCTAAACTCtt                                                                                                                                                                     >  3:284367/1‑132 (MQ=255)                                                                                gttGACGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGGACTCGATTGTTACTGCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCAc                                                                                                                                                                 <  3:476891/131‑1 (MQ=255)                                                                                 ttGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCAcc                                                                                                                                                                <  3:93602/132‑1 (MQ=255)                                                                                   ggCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAACCTGTTTCACCTCTTCTGGCGCACCGGCGAc                                                                                                                                            >  2:451241/1‑150 (MQ=255)                                                                                   ggCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCt                                                                                          <  1:396015/200‑1 (MQ=255)                                                                                    gCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACctct                                                                                                                                                             >  3:278139/1‑132 (MQ=255)                                                                                     cGGTAATCACCAGGTCAAATGTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTc                                                                                        <  1:520729/200‑1 (MQ=255)                                                                                       gTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCTGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCCCTTCTGGCGCACCGGCGACGTTa                                                                                                                                        >  2:122837/1‑150 (MQ=255)                                                                                       gTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCtcttct                                                                                                                                                          >  3:327743/1‑132 (MQ=255)                                                                                       gTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCg                                                                                                                                                 >  4:266135/1‑141 (MQ=255)                                                                                       gTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAg                                                                                      >  1:111526/1‑200 (MQ=255)                                                                                                 ggTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAAc                                                                                                                              <  2:164790/150‑1 (MQ=255)                                                                                                 ggTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACa                                                                            >  1:605443/1‑200 (MQ=255)                                                                                                 ggTCAAATTGCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAAc                                                                                                                              <  2:419833/150‑1 (MQ=255)                                                                                                  ttCAAATTTGTTCGCCTGCGGATGCTTGTACTCGGTTTTTACTTCATCTCCCAGCTGCGAGTTGAACCCGCGGCCCCTCGACTCCGTACGGGAAATCCCGCTAAACTGTTGCACCGCTACTGGCGCACCGGCGACGTTAAAGATGAAACt                                                                                                                             <  2:670665/149‑1 (MQ=255)                                                                                                  gTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACAt                                                                           >  1:127128/1‑200 (MQ=255)                                                                                                  gTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACAt                                                                           <  1:296124/200‑1 (MQ=255)                                                                                                      aaTTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACg                                                                                                                                           >  3:327820/1‑132 (MQ=255)                                                                                                      aaTTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATTAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGcc                                                                       <  1:368959/200‑1 (MQ=255)                                                                                                        tttCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGtt                                                                                                                                         <  3:579882/132‑1 (MQ=255)                                                                                                        gttcatcGGCCGGGGATGCTGGGAATCGATTTTTACTTCATCGCCCGGCTGCACGTGGTACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACAGGCGACAtt                                                                                                                                         <  3:529558/131‑1 (MQ=255)                                                                                                         ttcttcGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCt                                                                                                                      >  2:328924/1‑150 (MQ=255)                                                                                                         ttcttcGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCt                                                                                                                      <  2:756645/150‑1 (MQ=255)                                                                                                             tcGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTaa                                                                <  1:663896/200‑1 (MQ=255)                                                                                                             tcGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTaa                                                                <  1:254978/200‑1 (MQ=255)                                                                                                             gcGGCAGCGGAGTCTTGTACTCGATTTTTACTTAATCGCCCAGCTGCGCGTTGGACCAGCGGCCCAGCGACTCCGGACGGGAGATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGa                                                                                                                                    <  3:607865/131‑1 (MQ=255)                                                                                                               ggCAGCGGATGCTTGTACTCGATTTTTACGTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAg                                                                                                                <  2:20241/150‑1 (MQ=255)                                                                                                                   gCGTATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGAGGAAAc                                                                                                                              <  3:212176/132‑1 (MQ=255)                                                                                                                   gCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAAc                                                                                                                              >  3:518336/1‑132 (MQ=255)                                                                                                                     ggATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCAt                                                        >  1:189276/1‑200 (MQ=255)                                                                                                                        tGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCg                                                                                                       <  2:243703/150‑1 (MQ=255)                                                                                                                          cctGTACTCGATTTGTACTTCAGCGCCCATCTGCCCGTTGGCCCAGCGGCCCCGCGACTCCCGACGGCAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGc                                                                                                                       <  3:221575/130‑1 (MQ=255)                                                                                                                          cTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCg                                                                                                              >  4:520685/1‑141 (MQ=255)                                                                                                                          cTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGATTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCg                                                                                                              >  4:574024/1‑141 (MQ=255)                                                                                                                              tACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGAc                                               <  1:43613/200‑1 (MQ=255)                                                                                                                                 tCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAg                                                                                                                <  3:388595/132‑1 (MQ=255)                                                                                                                                  cGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATcaacaa                                                                                             >  2:461741/1‑150 (MQ=255)                                                                                                                                  cGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTa                                           >  1:328096/1‑200 (MQ=255)                                                                                                                                  cGATTTTTACTTAATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCTCCTCTTCTGGCGCACCGGCGTCGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACTCGCCATGTCGACGACAGTa                                           >  1:466757/1‑200 (MQ=255)                                                                                                                                    aTTTTTACTTCCTCGCCCCACTTCGCGTTGGACCCGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAGAGATGAACCTGTCGGCTTTGTAGCGAATATCGTTa                                                                                                    <  4:401255/141‑1 (MQ=255)                                                                                                                                    aTTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATc                                         >  1:635303/1‑200 (MQ=255)                                                                                                                                          aCTTCATCGCCCAGCTTCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCg                                                                                                       <  3:284138/132‑1 (MQ=255)                                                                                                                                          aCTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGc                                                                                     <  2:77591/150‑1 (MQ=255)                                                                                                                                          aCTTCATCGACCAGCTGCGCATTGGACCAGCGGCCCGGCGACTCCGGACGGCAAATCCCTCTAAACTGTTTCACCTCTTCTGGCGCACCGGGGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGc                                                                                     <  2:280627/150‑1 (MQ=255)                                                                                                                                             tCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATc                                                                                           >  4:296287/1‑141 (MQ=255)                                                                                                                                             tCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATCTTTAAATGc                                >  1:731029/1‑200 (MQ=255)                                                                                                                                             tCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATCTTTAAATGc                                >  1:147959/1‑200 (MQ=255)                                                                                                                                                  gCCCAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATcaac                                                                                               <  3:360612/132‑1 (MQ=255)                                                                                                                                                   cccAGCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACa                                                                            <  2:225344/150‑1 (MQ=255)                                                                                                                                                       gCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCACCAATCt                                                                                          >  3:70925/1‑132 (MQ=255)                                                                                                                                                       gCTGCGCGTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCt                                                                                          >  3:497217/1‑132 (MQ=255)                                                                                                                                                         tgggcgTTGAACCGTCGGACCCGCGACTACGGACGGGAAATCGCGCTAAACTGTTTGACCTCTTCTGGCGCACCGGCGACGTTAAGGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCt                                                                      <  2:559807/147‑1 (MQ=255)                                                                                                                                                         tGCGCGGTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTc                                                                                        <  3:87932/132‑1 (MQ=255)                                                                                                                                                             cgTTGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACAGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGt                                                                  >  2:689944/1‑150 (MQ=255)                                                                                                                                                               tttgcctctctgCCCCGCTACTCCGGAAGTGTAATCCCGTTCATCAGTTTCACCTTTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAGCAATCGGCAGCGTATCGACATTGCCTTTGTaa                                                                <  2:758457/139‑1 (MQ=255)                                                                                                                                                               ttGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACa                                                                            >  1:805453/1‑138 (MQ=255)                                                                                                                                                               ttGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACa                                                                            <  2:805371/138‑1 (MQ=255)                                                                                                                                                               ttGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTaa                                                                <  2:505361/150‑1 (MQ=255)                                                                                                                                                               ttGGACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTaa                                                                <  2:459652/150‑1 (MQ=255)                                                                                                                                                               ttGGACCAGCGGCACCGCGACTCCGGACGGGAAATCCCGCTAAACTTGTTCTCCTCTTCTGGTGCACCGGCGACGTGAAAGATGAGACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTaa                                                                <  2:503363/150‑1 (MQ=255)                                                                                                                                                                 ggACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATc                                                                                >  3:535112/1‑132 (MQ=255)                                                                                                                                                                 ggACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGcc                                                                       >  4:93042/1‑141 (MQ=255)                                                                                                                                                                 ggACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCAtgttg            >  1:372772/1‑200 (MQ=255)                                                                                                                                                                  gACCAGCGGCCCCGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCAtgttgt           <  1:736866/200‑1 (MQ=255)                                                                                                                                                                    ccAGCGGCCCCGCGACTCCGGACGGGAAACCCCGCTAAACTCTTTCACCTCTTCTGGCACACCGGCAACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGAc                                                                             <  3:510528/132‑1 (MQ=255)                                                                                                                                                                           ccccGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAAc  >  1:283318/1‑200 (MQ=255)                                                                                                                                                                           ccccGCGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTAACGACATTGCCTTTGTAACGCGCCATGTCg                                                    >  2:297693/1‑150 (MQ=255)                                                                                                                                                                               |                                                                                                                                                                                                    CTTCATTGCCCAGCACAAGAGTTTGTTCTTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGACATTGCCTTTGTAACGCGCCATGTCGATGACCGTATCTTTAAATGCCGTTTTGCCCTGCCATGTTGTTTTGTCAAC  >  NC_000913/4597314‑4597683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.