Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 516,362 T→C intergenic (+193/+313) JCVSYN2_02375 → / ← JCVSYN2_02380 antitermination protein NusG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816516,3620TC99.3% 13288.8 / NA 3550intergenic (+193/+313)JCVSYN2_02375/JCVSYN2_02380antitermination protein NusG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/3);  major base C (1628/1890);  minor base A (17/8);  total (1647/1903)
Fisher's exact test for biased strand distribution p-value = 4.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3585 total aligned reads displayed.

AAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑AAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAA  >  CP016816/516237‑516486
                                                                                                                              |                                                                                                                            
 aaCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACAtt                                                                                                                           >  2:2011464/1‑128 (MQ=255)
  aCATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACAttt                                                                                                                          >  1:711857/1‑128 (MQ=255)
     tAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAg                                                                                                                       <  2:1899630/128‑1 (MQ=255)
      aaaaTATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGt                                                                                                                      >  2:5480745/1‑128 (MQ=255)
         atatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTAta                                                                                                                   >  1:4353903/1‑127 (MQ=255)
          tatatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTAttt                                                                                                                  >  1:4200926/1‑128 (MQ=255)
           atatTTTTAAATAATATGTTTTTATAAGATTAGAAAATAAGCTTATTTTAATGGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTg                                                                                                                 <  1:649117/128‑1 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTTTTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTATTTAAAATTTTAA‑TAAAAAAACTTTTTAGTAtttc                                                                                                                 >  2:2294379/1‑127 (MQ=255)
           atatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTg                                                                                                                 >  2:5728165/1‑128 (MQ=255)
            tatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGc                                                                                                                >  1:2262601/1‑128 (MQ=255)
            tatTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGc                                                                                                                >  2:3872348/1‑128 (MQ=255)
              tttttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCaa                                                                                                              <  2:3698802/128‑1 (MQ=255)
                tttAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAAtt                                                                                                            <  2:4636698/128‑1 (MQ=255)
                  tAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATtat                                                                                                          <  1:2375692/128‑1 (MQ=255)
                  tAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATtat                                                                                                          >  1:2689995/1‑128 (MQ=255)
                   aaaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTCCAATtata                                                                                                         >  2:1235246/1‑128 (MQ=255)
                    aaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGTCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATaa                                                                                                        <  2:4204855/128‑1 (MQ=255)
                    aaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATaa                                                                                                        <  2:5598943/128‑1 (MQ=255)
                    aaaaaTATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATaa                                                                                                        <  1:5463454/128‑1 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                  >  2:5969544/1‑128 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                  <  2:1112109/128‑1 (MQ=255)
                          atGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                  >  2:3585179/1‑128 (MQ=255)
                          atGTTTATATAAGATTAGAACAACAGCTTATTTTATGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGt                                                                                                  <  2:533790/128‑1 (MQ=255)
                           tatttttATAAGATTAGAAAAAAAGGTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTATAATTTTAA‑TTAAAAAACATTTTAGTATTTGCAATTATAAAAAAGtt                                                                                                 <  1:1295087/126‑1 (MQ=255)
                              ttttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAATAACATTTTAGTATTTGGAATTATAAAATAGTTTAt                                                                                              >  1:3350853/1‑128 (MQ=255)
                              ttttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTAt                                                                                              >  1:445110/1‑128 (MQ=255)
                               tttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATg                                                                                             <  2:2694814/128‑1 (MQ=255)
                                ttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGa                                                                                            <  2:2400375/128‑1 (MQ=255)
                                ttATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGa                                                                                            <  1:6212516/128‑1 (MQ=255)
                                   taAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataa                                                                                         <  2:293081/128‑1 (MQ=255)
                                   taAGATTAGAAAAAAAGCTTATTTTAAGGGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataa                                                                                         <  1:4731929/128‑1 (MQ=255)
                                     aGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGataata                                                                                       <  1:5692916/128‑1 (MQ=255)
                                       aTTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAAtata                                                                                     <  2:1722185/128‑1 (MQ=255)
                                        ttAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATATTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATAc                                                                                    <  1:2813722/128‑1 (MQ=255)
                                          aGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCt                                                                                  >  2:2497976/1‑128 (MQ=255)
                                           gAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTa                                                                                 >  1:2127617/1‑128 (MQ=255)
                                            aaaaaaaTCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAt                                                                                <  1:1164472/128‑1 (MQ=255)
                                             aaaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAtt                                                                               <  2:2811291/128‑1 (MQ=255)
                                              aaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATaaaaa                                                                                                     <  1:187710/105‑1 (MQ=255)
                                              aaaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTa                                                                              >  1:362839/1‑128 (MQ=255)
                                               aaaaGCTTATTTTAAGGGCTTTTTTTAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                             >  2:3246511/1‑128 (MQ=255)
                                               aaaaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTaa                                                                             >  2:3394943/1‑128 (MQ=255)
                                                 aaGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAata                                                                           <  1:5169794/128‑1 (MQ=255)
                                                  aGCTTATTTTAAGGGATTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAatat                                                                          <  2:4990380/128‑1 (MQ=255)
                                                   gCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAAtata                                                                         <  1:5293824/128‑1 (MQ=255)
                                                   gCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAAtata                                                                         >  1:155181/1‑128 (MQ=255)
                                                    cTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTAtt                                                                               <  1:2931968/121‑1 (MQ=255)
                                                    cTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAg                                                                        >  1:994717/1‑128 (MQ=255)
                                                     ttATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGa                                                                       >  1:165986/1‑128 (MQ=255)
                                                        ttttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATa                                                                    >  2:510284/1‑128 (MQ=255)
                                                         tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                                   <  2:1479825/128‑1 (MQ=255)
                                                         tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                                   <  1:2006455/128‑1 (MQ=255)
                                                         tttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaa                                                                   <  1:1004051/128‑1 (MQ=255)
                                                          ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                                  >  2:3787237/1‑128 (MQ=255)
                                                          ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                                  <  2:3481263/128‑1 (MQ=255)
                                                          ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                                  >  1:5914241/1‑128 (MQ=255)
                                                          ttAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATaaa                                                                  >  1:3423757/1‑128 (MQ=255)
                                                           tAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaac                                                                 >  1:4070229/1‑128 (MQ=255)
                                                           tAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaac                                                                 <  2:4079316/128‑1 (MQ=255)
                                                            aaGGTCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTATTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaaca                                                                <  2:6207121/128‑1 (MQ=255)
                                                            aaGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAATATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAaaca                                                                <  1:2516287/128‑1 (MQ=255)
                                                                  ttttttAAAGATTATTTAATATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGa                                                          <  1:6373638/128‑1 (MQ=255)
                                                                  ttttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGa                                                          >  1:394029/1‑128 (MQ=255)
                                                                   tttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaa                                                         >  2:2131003/1‑128 (MQ=255)
                                                                    ttttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGaaa                                                        <  1:5594387/128‑1 (MQ=255)
                                                                      ttAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAATAAAAAATTTTAGTATTTTCAATTATAAAAAAGTTTATTATAATATACCTATTAATATAGAATAAACAACTAGAAAag                                                      >  1:4860447/1‑128 (MQ=255)
                                                                            attattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAat                                                <  1:4630838/128‑1 (MQ=255)
                                                                             ttattTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAata                                               >  1:3840032/1‑128 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             <  2:4893334/128‑1 (MQ=255)
                                                                               attTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatt                                             <  2:5865022/128‑1 (MQ=255)
                                                                                tttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatta                                            <  2:719898/128‑1 (MQ=255)
                                                                                 ttAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtattat                                           <  2:6587366/128‑1 (MQ=255)
                                                                                  tAAAATTTTGATTTTATTTTTAATTAAAATTTTAA‑TAATAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATattatt                                          <  2:6440781/128‑1 (MQ=255)
                                                                                      aTTTTGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAAtatta                                            >  1:830728/1‑122 (MQ=255)
                                                                                       ttttGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTAt                                     <  2:4383240/128‑1 (MQ=255)
                                                                                         ttGTTTTTATTTTTAATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGa                                   <  1:3503190/128‑1 (MQ=255)
                                                                                           ttttttatttttaATTAAAATTTTAATAAAAAAAACATTTTAGTATTTGATATTATAAAAAAGTTTATGATAATATACCTGTTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGAt                                  <  2:161312/127‑1 (MQ=255)
                                                                                             ttttatttttaATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGc                               <  1:6046997/128‑1 (MQ=255)
                                                                                              tttatttttaATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCg                              <  2:3980868/128‑1 (MQ=255)
                                                                                                 atttttaATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGATAAGAGAAATATTATTAGTATGATAGCGaat                           >  2:2590241/1‑128 (MQ=255)
                                                                                                 atttttaATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaat                           >  2:1356562/1‑128 (MQ=255)
                                                                                                 atttttaATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaat                           >  1:3722438/1‑128 (MQ=255)
                                                                                                   ttttaATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaatta                         >  2:6327965/1‑128 (MQ=255)
                                                                                                    tttaATTAAAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGaattaa                        <  1:3214907/128‑1 (MQ=255)
                                                                                                       aattaaAAATTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAttt                     <  1:5072311/128‑1 (MQ=255)
                                                                                                          taaAATTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTa                  <  2:3150623/128‑1 (MQ=255)
                                                                                                             aaTTTTAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATACAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTaaaa               >  1:6599477/1‑128 (MQ=255)
                                                                                                                tttAA‑TAAAAAAACATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAattt            >  2:4519446/1‑128 (MQ=255)
                                                                                                                    aataaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAatttatt         >  1:4963759/6‑128 (MQ=255)
                                                                                                                     ataaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAatttattt        <  1:3068290/124‑1 (MQ=255)
                                                                                                                      taaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTAtttt       <  2:4758750/125‑1 (MQ=255)
                                                                                                                      taaaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTAtttt       <  2:178360/125‑1 (MQ=255)
                                                                                                                        aaaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaa     >  1:1419675/2‑128 (MQ=255)
                                                                                                                         aaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATATCGAATTAATTTTTAAAATTTATTTtaat    >  2:851885/1‑128 (MQ=255)
                                                                                                                         aaaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaat    >  1:1792984/1‑128 (MQ=255)
                                                                                                                          aaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaata   >  2:5222772/1‑128 (MQ=255)
                                                                                                                          aaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaata   >  1:2601311/1‑128 (MQ=255)
                                                                                                                          aaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTtaata   >  1:5791922/1‑128 (MQ=255)
                                                                                                                          aaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATATATTttatta   >  2:3017871/1‑128 (MQ=255)
                                                                                                                          aaaaCATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAAATTTTAAAATTTATTTtaata   >  2:6096242/1‑128 (MQ=255)
                                                                                                                              |                                                                                                                            
AAACATAAAATATATTTTTAAAAAATATGTTTTTATAAGATTAGAAAAAAAGCTTATTTTAAGGGCTTTTTTAAAGATTATTTAAAATTTTGTTTTTATTTTTAATTAAAATTTTAA‑AAGAAAAATATTTTAGTATTTGCAATTATAAAAAAGTTTATGATAATATACCTATTAATATAGAATAAACAACTAGAAAAGAGAAATATTATTAGTATGATAGCGAATTAATTTTTAAAATTTATTTTAATAA  >  CP016816/516237‑516486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑