BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.5%	4079.5 / NA	1285	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (3/4); new base T (703/571); total (708/577)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.1%	3880.7 / NA	1285	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (7/17); new base T (701/560); total (708/577)
Fisher's exact test for biased strand distribution p-value = 1.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1338 total aligned reads displayed.

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420031‑420281

||

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑ttttaatttta                                                                                                                          >  1:567921/1‑125 (MQ=255)

        ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑ttttaattttaa                                                                                                                         >  2:718728/1‑124 (MQ=255)

        ttcattcaTACTTTTAGTATCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑ttttaattttaa                                                                                                                         <  1:2442016/128‑5 (MQ=255)

          cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATa                                                                                                                       <  2:1382134/128‑1 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTATTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAAc                                                                                                                     >  2:3180190/1‑128 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAAc                                                                                                                     >  2:2282833/1‑128 (MQ=255)

            ttcatACTTTGAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAAc                                                                                                                     <  2:2629547/128‑1 (MQ=255)

             tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACt                                                                                                                    >  1:3585080/1‑128 (MQ=255)

              catACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAATACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTg                                                                                                                   >  2:3490733/1‑128 (MQ=255)

               atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATTTTTGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGa                                                                                                                  >  2:2034400/1‑128 (MQ=255)

               atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGa                                                                                                                  >  1:1068061/1‑128 (MQ=255)

                tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGaa                                                                                                                 <  1:533552/128‑1 (MQ=255)

                tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGaa                                                                                                                 >  2:1875812/1‑128 (MQ=255)

                tACTTTTAGTATCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGaa                                                                                                                 <  2:1139017/128‑1 (MQ=255)

                 aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTATAAAACTACTATAACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAat                                                                                                                >  1:119690/1‑128 (MQ=255)

                 aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAat                                                                                                                <  2:2136838/128‑1 (MQ=255)

                  cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAatt                                                                                                               <  2:2388431/128‑1 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAatta                                                                                                              >  1:2894717/1‑128 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTATTAAAAAATATTTAAATT‑AAATAACTGAatta                                                                                                              >  1:1936154/1‑128 (MQ=255)

                     ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAatta                                                                                                              >  2:1064120/1‑126 (MQ=255)

                      tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTAttt                                                                                                           >  1:3066795/1‑128 (MQ=255)

                      tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAAATGAATTAttt                                                                                                           >  1:2299490/1‑128 (MQ=255)

                       aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATAAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTAtttt                                                                                                          <  2:148791/128‑1 (MQ=255)

                        gTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAAc                                                                                                                     <  2:2757211/116‑1 (MQ=255)

                          ctcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCa                                                                                                       <  1:3745536/128‑1 (MQ=255)

                           tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                      <  1:1803316/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAATATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                    <  2:57599/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAAATCCTACAACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCaatt                                                                                                    >  1:1954922/1‑126 (MQ=255)

                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                   <  1:2515146/128‑1 (MQ=255)

                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                   <  1:2063966/128‑1 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                 >  1:409842/1‑128 (MQ=255)

                                 tccgacctttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                <  1:2981790/121‑1 (MQ=255)

                                 aTCTTAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTGTTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGtt                                                                                                <  2:3617811/128‑1 (MQ=255)

                                    tAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTaaa                                                                                             <  1:633751/128‑1 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAatta                                                                                          >  2:3061397/1‑128 (MQ=255)

                                        tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                         <  1:3447292/128‑1 (MQ=255)

                                        tttCTAAAATTATTTTATAATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattat                                                                                         <  2:3884724/128‑1 (MQ=255)

                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                        >  1:2801121/1‑128 (MQ=255)

                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAattatt                                                                                        >  1:3620236/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                      >  1:3286632/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                      >  1:2411642/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                      >  2:370084/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                      >  1:2722285/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTAtttt                                                                                      >  2:3399142/1‑128 (MQ=255)

                                            tAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTc                                                                                     <  2:294190/128‑1 (MQ=255)

                                            tAAAATTATTTTATCATATAAAAAAATAATACTTTTATTAGTTGATATATTAGAATACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTCTTAAATTATTTTc                                                                                     <  2:176810/128‑1 (MQ=255)

                                              aaaTTATTTTATCATTTAAAAATATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                   <  1:924173/128‑1 (MQ=255)

                                              aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaa                                                                                   >  1:1401489/1‑128 (MQ=255)

                                               aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCaaa                                                                                  >  2:1317057/1‑128 (MQ=255)

                                                attattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAAt                                                                                 >  2:1993831/1‑128 (MQ=255)

                                                 ttattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTATAATAACTGAATAATTTTTCAAATTGTTAAATTATTTTCAAATc                                                                                >  1:3397343/1‑128 (MQ=255)

                                                  tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCa                                                                               >  2:1951294/1‑128 (MQ=255)

                                                   attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCAt                                                                              >  1:3680413/1‑128 (MQ=255)

                                                      ttATCATTTAAAAAAATAATACTTTAATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTAt                                                                           <  2:3765600/128‑1 (MQ=255)

                                                       tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                          <  2:3340908/128‑1 (MQ=255)

                                                        ataaTTTAAAAAACTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                         <  1:467373/125‑1 (MQ=255)

                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                         >  2:3826678/1‑128 (MQ=255)

                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                         <  1:2856770/128‑1 (MQ=255)

                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCt                                                                         <  1:3534555/128‑1 (MQ=255)

                                                         tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑ATTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTa                                                                        <  1:2585395/128‑1 (MQ=255)

                                                          cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTAt                                                                       >  1:3848186/1‑128 (MQ=255)

                                                             ttAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTaa                                                                    >  2:1000360/1‑128 (MQ=255)

                                                              tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATc                                                                          <  1:738438/121‑1 (MQ=255)

                                                              tAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAAt                                                                   >  2:157183/1‑128 (MQ=255)

                                                                 aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa‑‑a                                                              <  2:2234779/128‑1 (MQ=255)

                                                                 aaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCa‑‑a                                                              <  2:922886/128‑1 (MQ=255)

                                                                  aaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑At                                                             >  1:1636258/1‑128 (MQ=255)

                                                                   aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATATTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑Att                                                            >  1:3363386/1‑128 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑Attt                                                           <  1:2222041/128‑1 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑Attt                                                           <  2:2885305/128‑1 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATAGTTAAATTATTTTCAAATAATTATCTATTAATCA‑‑Attt                                                           >  1:1866616/1‑128 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGAACTTTTTTAAAAAAT‑TTTTAATTTTATTAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑Attt                                                           <  1:2655270/128‑1 (MQ=255)

                                                                       aataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTAt                                                        <  1:1266957/128‑1 (MQ=255)

                                                                         taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGc                                                      <  1:1000360/128‑1 (MQ=255)

                                                                            ttttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑ct                                                  >  1:1449297/1‑127 (MQ=255)

                                                                             tttATTAGTTTATAAATTAGAAAACTACTACCACTTTTTTAAAAAAT‑TTTTAATTTTAATAAATGAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCG‑CG‑t                                                <  1:1716848/128‑1 (MQ=255)

                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGAT‑ctt                                                 >  1:2174725/1‑126 (MQ=255)

                                                                              ttatttGTTGATATATTAGAAAACTACTAGCACTTATTTAAAAAAT‑TTTTAATATTAATAACTGAATTATTTTTCAAATAGTTAAATTATATTCAAATCATAATCTATAAATCA‑‑ATTTTTTGCT‑CG‑tt                                               >  2:3549583/1‑128 (MQ=255)

                                                                              ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATTCT‑Ct‑tt                                               >  1:3233015/1‑128 (MQ=255)

                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CG‑ttt                                              >  2:2538213/1‑128 (MQ=255)

                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTATTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGAT‑AG‑ttt                                              >  1:863408/1‑128 (MQ=255)

                                                                                attaGTTGATATAATAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CG‑tttt                                             >  2:123640/1‑128 (MQ=255)

                                                                                         tatatTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTTAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATATATTAATCA‑‑ATTTTATTCT‑Ct‑ttttttttttttt                                    >  2:312133/1‑128 (MQ=255)

                                                                                         tatatTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATATTAATCTATTAATCA‑‑ATATTATTAT‑CG‑atttttttttttt                                    >  1:2022512/1‑128 (MQ=255)

                                                                                          atatTATAAAACTAATATAAATTTTTTAAAAAAT‑TTTTAATTTTAAAAACATAAATATTTTTAAAATTTTTAAATTATTTTAAAATAAATATCTATTAAAAA‑‑ATTTTATTCT‑CG‑tttttttttttttt                                   <  1:3495106/128‑1 (MQ=255)

                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CG‑tttttttttttttt                                   >  2:779752/1‑128 (MQ=255)

                                                                                             ttAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CG‑ttttttttttttttttt                                >  1:1583599/1‑128 (MQ=255)

                                                                                               aGAAAACTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CG‑tttttttatttttttttta                              >  1:3170115/1‑126 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGAT‑CG‑ttttttttttttttttataaaa                           >  1:681944/1‑122 (MQ=255)

                                                                                                   atataataataCTTTTTTAAAAATA‑TTTTAATTTTTATAAATTTATTTTTTTTTAAATTTTTTATTTTTTTTAAAATAATTTTTTTTTAATAA‑‑ATTTTATGCT‑GGTTTTTTTTTTTTTTTTTTACaaa                           <  2:480099/118‑1 (MQ=255)

                                                                                                     ctactaGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CGTTTTTTTTTTTTTTTTTTACaaaaa                         >  1:3794527/1‑128 (MQ=255)

                                                                                                          aGCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CGTTTTTTTTTTTTTTTTTTAAAAAAATATaa                    >  1:1351681/1‑128 (MQ=255)

                                                                                                           gCACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CGTTTTTTTTTTTTTTTTTTAAAAAAATATaaa                   >  2:858675/1‑127 (MQ=255)

                                                                                                            tatttttttTAAAAAT‑TTTTTATTTTATTAAATTTATTTTTTTTTAAATTTTTAAATTTTTTTAAAATTATTTTTTTTTAATAATTTTTTTTTTTT‑TT‑TTTTTTTTTTTTTTTTTACAAAAATATAat                   <  2:164502/125‑1 (MQ=255)

                                                                                                            cACTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGAT‑CG‑TTTTTTTTTTTTTTTTTACTAAAATATAattt                 >  2:3260740/1‑128 (MQ=255)

                                                                                                              tttttttAAAAAAT‑TTTTAATTTTTATAAATTAATTTTTTTTTAAATTTTTTAATTATTTTAAAATAATTATATATTAATCA‑‑ATTTTATTCT‑CG‑TTTTTTTTTTTTTTTTTACAAAAATATAatttat               <  1:788199/127‑1 (MQ=255)

                                                                                                              cTTTTTTAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CGTTTTTTTTTTTTTTTTTTAAAAAAAAATAattta                >  1:3126509/1‑128 (MQ=255)

                                                                                                              attttttTAAAAAT‑TTTTAATTTTTATAAATTTATTTTTTTTTTAATTTTTTATTTATTTTTAAATTATTTTTTATTTATAA‑‑ATTTTATTCTATT‑TTTTTTTTTTTTTTTTTACAAAAATATAattta                <  1:1121999/127‑1 (MQ=255)

                                                                                                               ttttttAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CG‑TTTTTTTTTTTTTTTTTAAAAAAATATAatttatt              >  1:152281/1‑128 (MQ=255)

                                                                                                                    tAAAAAAT‑TTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CGTTTTTTTTTTTTTTTTTTAAAAAAATATAAtttattttat          >  1:21164/1‑128 (MQ=255)

||

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAAT‑TTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCA‑‑ATTTTATGCT‑CG‑TTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTA  >  CP016816/420031‑420281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`