BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	165,218	A→T	intergenic (+182/‑360)	JCVSYN2_00665 → / → JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	165,218	0	A	T	97.6%	10777.4 / 4.7	3730	intergenic (+182/‑360)	JCVSYN2_00665/JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base A (17/73); new base T (2442/1198); total (2459/1271)
Fisher's exact test for biased strand distribution p-value = 2.21e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3870 total aligned reads displayed.

ATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165092‑165346

    aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                                   <  2:1720627/128‑3 (MQ=255)

    aCTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTtaaaaa                                                                                                                                   <  2:6593328/128‑3 (MQ=255)

     cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                  >  2:4243066/1‑128 (MQ=255)

      tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTGGTAAGAAACTAAAACTATTATCTTTTTAGATTTTTTTTGTATATGCTTATTTTTAAAAaat                                                                                                                                 >  1:9596813/1‑128 (MQ=255)

      tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                                 >  2:2068069/1‑128 (MQ=255)

         aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                              >  2:5656431/1‑128 (MQ=255)

            agTATTACTTTACTAGATGATAATATTTAGTTTCATTAATATATCGCACTTGTATGAAATAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTTTATATGCAAATTTTTAAAAAATTAAttt                                                                                                                           <  2:8976884/127‑1 (MQ=255)

               aTTACTTTACTAGTTGATAATATTTAGTTTCATTAGTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                        <  1:3025093/128‑1 (MQ=255)

               aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                        <  2:5880538/128‑1 (MQ=255)

               aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                        <  1:3656642/128‑1 (MQ=255)

                    tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGt                                                                                                                   >  1:5883200/1‑128 (MQ=255)

                     ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                  <  1:5241460/128‑1 (MQ=255)

                     ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                  >  2:3073009/1‑128 (MQ=255)

                     ttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTa                                                                                                                  >  1:3454511/1‑128 (MQ=255)

                      tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                 >  1:943832/1‑128 (MQ=255)

                       actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                                >  2:532014/1‑128 (MQ=255)

                         tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                              >  1:7837640/1‑128 (MQ=255)

                           gTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATTAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑t                                                                                                           <  2:10429173/128‑1 (MQ=255)

                                 aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑T‑C‑‑ttt                                                                                                 >  1:3211502/1‑128 (MQ=255)

                                 aataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑ttt                                                                                                 >  1:8890121/1‑128 (MQ=255)

                                  atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑tttt                                                                                                >  1:9924011/1‑128 (MQ=255)

                                  atatTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑tttt                                                                                                >  1:8017761/1‑128 (MQ=255)

                                    atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑tttttt                                                                                              >  2:9369025/1‑128 (MQ=255)

                                     tttAGTTTCATTAAATTAACCCACTTTTTATAATTACTAATAAACTAAAACTATTATCTTTTTATAAATTTTTTGTATAAGCAAAATTTTAAAAAAATAATTTTAACATGTAAAAAA‑AA‑C‑C‑‑ttttttt                                                                                             <  1:2650095/128‑1 (MQ=255)

                                       tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑ttttttttt                                                                                           >  2:6910158/1‑128 (MQ=255)

                                       tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑ttttttttt                                                                                           >  1:19768/1‑128 (MQ=255)

                                       tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑ttttttttt                                                                                           >  1:1112931/1‑128 (MQ=255)

                                         gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑ttttttttttt                                                                                         >  1:2835609/1‑128 (MQ=255)

                                          tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCATATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑tttttttttttt                                                                                        >  2:6751906/1‑128 (MQ=255)

                                           ttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑tttttttttttta                                                                                       >  2:8848308/1‑127 (MQ=255)

                                            tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑tttttttttttttt                                                                                      >  2:10247892/1‑128 (MQ=255)

                                              aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑tttttttttttttttt                                                                                    >  2:7738296/1‑128 (MQ=255)

                                              aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑tttttttttttttttt                                                                                    >  1:9262166/1‑128 (MQ=255)

                                                tAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑tttttttttttttttttt                                                                                  >  2:271281/1‑128 (MQ=255)

                                                  atttttgttatttGTATTAATTTGTAATAAAAAAAAAATTTTATTTTTTTATATATTTTTTTTTTTTGCAAAATTTTAAAAAATTAATTTTAAATTTTAAAAAA‑AA‑C‑C‑‑TTTTTTTTTTTTTTTTTTta                                                                                <  1:6820662/117‑1 (MQ=255)

                                                   tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑T‑A‑‑ttttttttttttttttttaaa                                                                               >  2:1534092/1‑125 (MQ=255)

                                                   tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTtaa                                                                               >  1:1430138/1‑127 (MQ=255)

                                                   tttATAGCTATTTTATTAAATAATAATAAAATAAAAAAATTAACTTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAA‑AA‑C‑C‑‑cttttttttttttttttttta                                                                               <  2:4981424/128‑3 (MQ=255)

                                                        cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑T‑CT‑TTTTTTTTTTTTTTTTTTTAT‑AAta                                                                          >  2:6386896/1‑127 (MQ=255)

                                                        cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AAtat                                                                         >  1:8172802/1‑126 (MQ=255)

                                                        cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTATAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑A‑‑TATTTTTTTTTTTTTTTTTAT‑AAtat                                                                         >  1:5714087/1‑126 (MQ=255)

                                                            cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑ttttttttttttttttttttaatataaatt                                                                      >  1:4117252/1‑117 (MQ=255)

                                                             ttGTATTAATTTTTAATAAACTAAAACTATTATTTTTTTATTTATTTTTTTTTTATTCAAATTTTTAAAAAAATAATTTTAAAATTTAAAAAA‑TA‑T‑C‑‑‑TTTTTTTTTTTTTTTTTTAT‑AATGGAAatat                                                                   <  2:4631460/128‑1 (MQ=255)

                                                             ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCTAATTTTTAAAAAATTAAATTGAACTTATAAAAAA‑TA‑T‑C‑‑TTTTTTTTTTTTTTTTTTTAA‑AATGAAAatt                                                                    >  1:1763768/1‑127 (MQ=255)

                                                             ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGAAAata                                                                    >  1:6065718/1‑128 (MQ=255)

                                                             ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAA‑AATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATTGAAatat                                                                   >  2:8327537/1‑128 (MQ=255)

                                                                tATTAATTAGTAATTAATTAAAAATTTTTTTTTTTTATTTATTTTTTTTTTATTTAAATTTTTTAAAAATTAATTTTAAATTTTAAAAAA‑AA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATaa                                                                 <  2:7541935/128‑1 (MQ=255)

                                                                tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTTT‑TATTTAAAAATaa                                                                 >  1:10007984/1‑128 (MQ=255)

                                                                  tGAAATAGTAATAAAATAAAATTATTTAATTTTTATAATTTTTTTTTTTATGCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAA‑TA‑G‑C‑‑‑TTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAAtt                                                              <  2:6049786/128‑1 (MQ=255)

                                                                   ttattattAATAAAATAAAAATATTTACTTTTTATTTTTTTTTTTTATATTCAAATTTTTAAAAAATTTATTTTAACTTTTAAAAAA‑AA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAAtt                                                              <  2:8064401/126‑1 (MQ=255)

                                                                   ttattattAATAAAATAAAAAAAATTTATTTTTATATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTTACTTTTAAAAAA‑TA‑T‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAAtt                                                              <  2:4783527/126‑1 (MQ=255)

                                                                    aaTTATTAATAAAATAAAAAAATTATTTTTTTTTATTTTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAA‑AA‑T‑CT‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAAtt                                                              <  1:9087653/128‑1 (MQ=255)

                                                                    aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTTT‑ATTTTAAATATAAtttt                                                             >  1:8489509/1‑128 (MQ=255)

                                                                     aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑T‑C‑‑‑TTTTTTTTTTTTTTTTTTAA‑AATTTAAATATAAATTTTa                                                           >  1:10182943/1‑128 (MQ=255)

                                                                     aTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATTTAATAATAAAtttt                                                            >  1:2376845/1‑128 (MQ=255)

                                                                      ttAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑CT‑TTTTTTTTTTTTTTTTTTTAT‑ATTTTAAAAAAAAAtttt                                                            >  2:2937624/1‑128 (MQ=255)

                                                                       tAGTTATAAAATAAAAATATTATATTTTTATATATTTTTTTTAAATGCAAATTTTTAAAAAATTAAATTGTAATTTTAAAAAA‑TA‑G‑C‑‑‑‑TTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGt                                                        <  1:5526696/128‑1 (MQ=255)

                                                                       tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATTTAAATAAAAATTTTAg                                                          >  2:1332093/1‑128 (MQ=255)

                                                                         gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCTAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑CT‑TTTTTTTTTTTTTTTTTTTAA‑AATTAAAAAATAAAtttttat                                                         >  1:6575703/1‑125 (MQ=255)

                                                                         gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑‑TTTTTTTTTTTTTTTTTTAT‑AATGTAAATAAAAATTTTAgtat                                                       >  1:3831412/1‑125 (MQ=255)

                                                                           aatatactaaaataaTTATTTTTTTTTATATTTTTTTTTTATTCAAATTTTTAAAAAATTATTTTTAACTTTTAAAAAA‑TA‑T‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTAt                                                      <  2:1147065/123‑1 (MQ=255)

                                                                           aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTA‑‑AATGTAAAATAAAATTTTAgttttt                                                     >  1:704716/1‑123 (MQ=255)

                                                                               aaataaaaaatttatATTTTTATTTATTTTTTTTATATTTAAATTTTTTAAAAATTTATTTTAAATTTTTAAAAA‑TT‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTttg                                                  <  1:9761879/117‑1 (MQ=255)

                                                                                  taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑CT‑TTTTTTTTTTTTTTTTTTTAT‑ATTTAAAATAAAAATTTTattaattttttt                                                >  2:4004380/1‑117 (MQ=255)

                                                                                    atatttttttttttttATTTTTTTTTTTTTTATTTAAATTTTTTTAAAATTTATTTTAAATTTTAAAAAATTT‑T‑T‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTtgttgt                                              <  1:7499784/117‑1 (MQ=255)

                                                                                    aaaattttatttttttttttatttttttttTTTTTAAATTTTTTAAAAATTTATTTTTAATTTTTAAAAA‑TT‑T‑T‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTc                                             <  1:5040921/122‑1 (MQ=255)

                                                                                     aaattttttttttttttatattttttttatatTTAAATTTTTAAAAAATTAATTTTTAATTTTTAAAAA‑‑A‑A‑T‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                           <  2:2399568/118‑1 (MQ=255)

                                                                                      attattatATTTTTTTTTATTTTTTTTATATTTAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAA‑TA‑‑‑T‑‑ATTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                          <  2:7117689/126‑1 (MQ=255)

                                                                                      attattatATTTTTATATATTTTTTTTTTATTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAA‑TT‑T‑C‑‑‑TTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                          <  2:5144742/126‑1 (MQ=255)

                                                                                      aatattatATTTTTATTTATTTTTTTTTTTTTCAAATTTTTAAAAAATTTATTTGAAATTTTTAAAAA‑TA‑T‑C‑‑‑TTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTaa                                          <  2:2582571/126‑1 (MQ=255)

                                                                                        tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑GAT‑‑TTTTTTTTTTTTTTTTTTTTT‑TATTTTAAAATAAATTTTATTTAttttttgttttat                                          >  1:5393723/1‑119 (MQ=255)

                                                                                        tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑CT‑TTTTTTTTTTTTTTTTTTTAA‑AATTTAAATATAAATTTTATTTATttttttttaaaa                                          >  2:820508/1‑122 (MQ=255)

                                                                                        tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑ATTGGAAATATAAATTTTTTGTTTTTtgttgtgaatg                                         >  2:9800421/1‑123 (MQ=255)

                                                                                          ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAA‑AATGTAAAAATAAATTTTAGATAATTTTTTTTATAATTa                                       >  2:6222424/1‑128 (MQ=255)

                                                                                          ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTT‑T‑ATTGTAAAAATAAATTTTATTTATTTTTTTTTATAATTAt                                      >  1:4853003/1‑128 (MQ=255)

                                                                                          ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑‑TTTTTTTTTTTTTTTTATAT‑AATGGAAATATAAATTTTTGGTATTTTTTTTTATAATTAt                                      >  1:103969/1‑128 (MQ=255)

                                                                                           tttttttttATATTTTTTTTTTATTTTTAAATTTTTTAAAAATTTATTTTTAATTTTAAAAAA‑TA‑T‑T‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                      <  1:2180696/124‑1 (MQ=255)

                                                                                           tttttttttAGTTATTTTTTTTTTATTTAAATTTTTAAAAAATTATTTTTAAATTTTAAAAAA‑TA‑T‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTAt                                      <  2:4443700/124‑1 (MQ=255)

                                                                                           tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑‑TTTTTTTTTTTTTTTTTTAA‑AATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATc                                     >  2:8505567/1‑128 (MQ=255)

                                                                                           tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑A‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAAAATAAATTTAAGTAATtttttttaaaaattaa                                      >  2:3464041/1‑118 (MQ=255)

                                                                                                ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTTTAAATGTAAATAAAATTTTAATGTTTTTTTTTGTATaataaaaaaa                                  >  1:635246/1‑120 (MQ=255)

                                                                                                ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTTT‑AATGTAATTATAAATTTTATGTATTTTGTTTTATAAGTTTATaaa                                 >  2:9139365/1‑128 (MQ=255)

                                                                                                    ttttatTTTTTTTATTTTGTAATTTTTTAAAAATTTATTTTTAATTTTTAAAAA‑TT‑T‑T‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                             <  1:4689026/125‑1 (MQ=255)

                                                                                                      atatTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAA‑AATTTAAATAAAAATTTTATTTTTTTTTTTTTATAATTAAAAAAAAAAATa                           >  1:8322259/1‑128 (MQ=255)

                                                                                                         ttttttGTATTTTAATTTTTTTATAAAATTAATTTTAAATTTTAAAAAA‑TATT‑T‑‑TTTTTTTTTTTTTTTTTTTAT‑AATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                         <  2:1938068/128‑1 (MQ=255)

                                                                                                         ttttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑‑TTTTTTTTTTTTTTTTTTAT‑AATGTAAATTTAATTTTTATTTTTTTTGTTTTATAATTATGAAAAAAAATAGaaa                       >  2:9657579/1‑128 (MQ=255)

                                                                                                             ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTATAT‑AATTTAAATATAAATTTTAGTTATTTTTTTTTATATGTAAATAAAAAAAAATAAAGGa                    >  2:3260069/1‑128 (MQ=255)

                                                                                                             ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑‑‑TTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTTTGTTTTTTTTTGAATAATTTTATAAaataatttaaaaataa                  >  1:7276210/1‑118 (MQ=255)

                                                                                                                 ttttttaaaTTTTTAAAAAATTATTTTTTATTTTTAAAAAA‑T‑‑‑‑T‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  2:10086326/122‑1 (MQ=255)

                                                                                                                 atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑CT‑TTTTTTTTTTTTTTTTTTTAT‑ATTTGAAATAAAAATTTTTGTTATTTTGTTTTTAAATTATAAAAAAAAAtaaaaataaaaa                 >  1:4500458/1‑128 (MQ=255)

                                                                                                                  atttaattttttAAAAAATTTATTTTTAATTTTTAAAAAA‑T‑‑T‑T‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  1:1251359/120‑1 (MQ=255)

                                                                                                                   atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑CTTTTTTTTTTTTTTTTTTTTTAT‑AATTGAAATATAAATTTTATTTATTTTTTTGTCTAAGTAAAAAAAAAAATAAAAATGaaaaa                >  1:8665456/1‑128 (MQ=255)

                                                                                                                    ttcAAATTTTTAAAAAATTAAATTTAAATTTTAAAAAA‑TA‑T‑CT‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat              <  2:8684321/126‑1 (MQ=255)

                                                                                                                    tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTTT‑AATGTAAATTAAATTTTTAGTTTTTTTTTTTTATAATTATATAAAAAAATATAAATTAAAAAata             >  2:3628331/1‑128 (MQ=255)

                                                                                                                     gCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑CT‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTATAATTATATAAAAAAATAGAAATAAAAAAata             >  2:9501535/1‑128 (MQ=255)

                                                                                                                      cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAA‑AATTTAATTATAAATTTTTGTTATTTTTTTTTATAATTATAAAAAAAAATATAAATTAAAAAATAtt           >  2:7334656/1‑128 (MQ=255)

                                                                                                                      cAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑‑‑TTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGTTATTTTGTTTTATAAGTATCTAAAAAAATAAAAATAAAAAattatttt         >  2:7910212/1‑126 (MQ=255)

                                                                                                                       aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTTT‑AATGTAAATAAAAATTTTATTTATTTTTTTTTATAATTATAAAAAAAAAAAGAAAGAAAAAAAttttt          >  2:5478859/1‑127 (MQ=255)

                                                                                                                         aTTTTTAAAAAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑TTTGTAATTATAATTTTTTGTTTTTTTTTTTTATATTTTTAAAAAAAAAAATAAATTAAAAAAtttttat        >  2:2236583/1‑125 (MQ=255)

                                                                                                                          ttttAAAA‑AATTAATTTTTATTTTTAAAAAA‑TA‑T‑T‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa      <  2:3796746/128‑1 (MQ=255)

ATTTACTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAA‑TA‑G‑C‑‑TTTTTTTTTTTTTTTTTTTAT‑AATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGT  >  CP016816/165092‑165346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`