Predicted mutation
evidence seq id position mutation annotation gene description
RA CP016816 165,268 (T)19→18 intergenic (+232/‑310) JCVSYN2_00665 → / → JCVSYN2_00670 tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP016816165,2500T.83.5% 958.7 / 220.6 345intergenic (+214/‑328)JCVSYN2_00665/JCVSYN2_00670tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (25/32);  new base . (176/112);  total (201/144)
Fisher's exact test for biased strand distribution p-value = 1.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 443 total aligned reads displayed.

ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAA  >  CP016816/165123‑165376
                                                                                                                                 |                                                                                                                              
ataataTTTATTTTTATTAATTTATCTCAATTGTATTAATTAGTAAGAAAATAAAACTATTATCTTTTTATA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGCt                                                                                                                                <  1:1940662/128‑1 (MQ=255)
        tttttttATTATTTTATATCACTTTTATTAATTTTTAATAAACTAAAACTATTATCTTTTTATT‑AATTTTTTTTTTATGCAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttttttttt                                                                                                                        <  2:1421411/125‑1 (MQ=255)
        tAGGTTCATTAATTTATCGCACTTGTATTTATTACTAATAAAATAATAATTATATATTTTTTTA‑TATTTTTTTTAAATTCAAA‑TTTTTAAAAAATTAATTTGCACTTGTAAAAAATACCttttttttt                                                                                                                        <  1:1001110/128‑1 (MQ=255)
         tttttAATTAATTTATATCACTTTTTTTAATTATTAATAAAATAAAAATATTATCTTTTTATA‑TATTTTTTTTATATTCAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGCtttttttttt                                                                                                                       <  1:1195236/126‑1 (MQ=255)
         aggttCAATAAATTTTCTCACTTGTATGAATTATTAAGAAACTAAAACATTTATCTTTTTAGA‑TATTTTCTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACCTGTAAAAAATAGCtttttttttt                                                                                                                       <  2:469565/125‑1 (MQ=255)
          tttaaaTTAATTTTTCTCAATTTTATTAATTATTAACAAAAAAAAAATATTATATTTTTATA‑TATTTTTTTTATATGAAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCttttttttttt                                                                                                                      <  1:873459/123‑1 (MQ=255)
              taTTAATTTATCCCAATTGTATTAATTATTATCAAAATAAAACTATTATTTTTTTATA‑TTTTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAATATCttttttttttttttt                                                                                                                  <  1:778655/127‑1 (MQ=255)
               ttttaattATTGCACTTTTATGTATTAGTAATAATATAAAACTATTATGTTTTTATA‑TATTTTTTGTATATTCAAA‑TTTTTAAAAAATTAAATTTAACTTGTAAAAAATAGCttttttttttttttta                                                                                                                 <  2:1365683/122‑2 (MQ=255)
                ttAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttta                                                                                                                >  1:302912/1‑127 (MQ=255)
                ttAATTTATAGCACTTGTATTAATTATTAATAAAATAAAAATATTATATTTTTATA‑TATTTTTTGTATAATCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttta                                                                                                                <  1:2577976/128‑2 (MQ=255)
                 tAATTTATTGCACTTGTATGAATTAGTAATAAAATAAAACTTTTATATTTTTATA‑TATTTTTTGTATATTCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttta                                                                                                               <  2:1628809/128‑2 (MQ=255)
                   aTTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttataa                                                                                                             >  1:1883733/1‑124 (MQ=255)
                    tttATTGGAATTGTATGTATTATTAAGAAAAAAAAACTATTTTTTTTTTATA‑TATTTTTTTTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATCtttttttttttttttataatg                                                                                                            <  2:2425775/128‑7 (MQ=255)
                    tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTtataa                                                                                                            >  1:2977685/1‑127 (MQ=255)
                    tttATAGAACTTTTATGAATTATTAATAAATTAAAACTATTATTTTTTTATT‑TATTTTTTTTATATTCAAA‑TTTTTAAAAAATTAATTTTAAATTGTTAAAAATACCtttttttttttttttataatg                                                                                                            <  1:1411016/128‑7 (MQ=255)
                     ttATAGCACTTGTATTAATTATTAATAAACTAAAACTATTATCTTTTTATA‑TATTTTTTGTAAATGCAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAAAAGCtttttttttttttttataatgg                                                                                                           <  1:1078849/128‑8 (MQ=255)
                      tataGCAATTGTTTGTATTATTAATAAAATATAAAAATTATATTTTTATA‑TATTTTTTTTATATTCAAA‑TTTTTAAAAAATTTATTTTTACTTTTTAAAAATAGCttttttttttttttttataatgg                                                                                                          <  1:1745169/128‑8 (MQ=255)
                         cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttttttttttttttaaatgaaaa                                                                                                       >  1:1821186/1‑119 (MQ=255)
                           cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTTTATATTCAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGCTATTTTTTATTTTTTTtatagtaaaaaa                                                                                                     >  1:2179669/1‑120 (MQ=255)
                             cTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAatat                                                                                                 >  2:2659206/1‑128 (MQ=255)
                              ttGTATTAATTAGTAATTAAATTAAAATTTTATATTTTTATT‑TATTTTTTTTTTATGAAAT‑TTTTTAAAAAATTTATTTTATATTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                                              <  1:1783996/128‑1 (MQ=255)
                              ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttatttgaaatattaat                                                                                                  >  1:618740/1‑116 (MQ=255)
                               tttattaattattaatTAACTAAAAATTTTATTTTTTTTGA‑TATTTTTTTTTTATTAAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAA‑‑‑‑‑TTTTGTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                            <  1:2257829/126‑1 (MQ=255)
                               tttattaattattaatAAACTAAATATATTATCTTTTTTTA‑TATTTTTTTTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                                              <  1:835946/126‑1 (MQ=255)
                               tgttttaaTTAGTTAGAAAATAAAAATATTTTCTTTTTATT‑TTTTTTTTGTATTTGTAAA‑TTTTTAAAAAATTAATTTGTACTTGTATAAAA‑‑‑‑‑TTGGTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                            <  2:1305089/122‑1 (MQ=255)
                               tGTTTGAATTTTTAATTAAATAAAACTATTATATTTTTATT‑TATTTTTTGTATATGAAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                                              <  1:578191/128‑1 (MQ=255)
                                tttttaaTTATTAATAAAATAAAAATTTTATATTTTTATA‑TATTTTTTTTATATTCAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAAAA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATaaa                                                                                              <  1:116541/123‑1 (MQ=255)
                                ttattaattattaatAAAATAAAAATAATATTTTTTTATA‑TATTTTTTGTTTATTCAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAA‑‑‑‑TTTCTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                            <  1:700838/127‑1 (MQ=255)
                                ttATGAATAAGATAGTAAATAAAACTATATACTTTTTTTA‑TAATTTTTTTAAATGCAAA‑ATTTTAAAAAATTTATTTTAACTTGTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                            <  2:1676936/127‑1 (MQ=255)
                                gttttaaTTATTAATCAACTATAACTATTATATTTTTATT‑AATTTTTTTTATATTCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAAt                                                                                             <  1:1607333/123‑1 (MQ=255)
                                 tATTAATTAGTAATAAAATAAAAATATTATATTTTTATA‑TATTTTTTTTATATTAAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                                            <  2:2272113/128‑1 (MQ=255)
                                  ttGAATTTTTAATAAAATAAAACTATTATTTTTTTTTA‑TATTTTTTTTATATTCAAA‑TTTTTTAAAAATTTATTTTAAATTTTAAAAAATA‑‑‑‑TCTTTTTTTTTTTTTATATAATGGAAATATAAAtttt                                                                                          <  1:2245551/127‑1 (MQ=255)
                                   tttattattAATAAAATAAAAATATTATATTTTTATA‑TATTTTTTTTTAATTGAAA‑TTTTTTAAAAATTATTTTTAAATTTTTAAAAA‑‑‑‑TTGATTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                                                         <  2:2132383/125‑1 (MQ=255)
                                   ttaatttttAAGAAAAAAAAAATTTTTTTTTTTTATA‑TTTTTTTTGTATTTTCAAA‑TTTTTAAAAAATTAATTTGAAATTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                                                         <  1:2105554/120‑1 (MQ=255)
                                       ttattaATAAAATAAAACTATTATTTTTTTTTA‑TATTTTTTTTATATTAAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                                                      <  2:1973048/128‑1 (MQ=255)
                                       ttACTAATAAATAAAAACTATTATTTTTTTAGA‑TATTTTTTGTATATGTATT‑TTTTTAAAAATTTAATTTTAAATTTTAAAAA‑‑‑‑‑TTATCCTTTTTTTTTTTTTATAATGGTAATATAAATTTTAGGTAt                                                                                    <  2:773405/128‑1 (MQ=255)
                                        ttttaataaACTAAAAATATTATTTTTTTAGA‑TTTTTTTTTTATATTTAAA‑TTTTTTAAAAATTAATTTTAAATTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                                                    <  1:1391514/125‑1 (MQ=255)
                                        tAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGATAATTAAAtttttgtt                                                                                      >  1:2120916/1‑124 (MQ=255)
                                           taataaAATAAAAATTTTATTTTTTTATA‑TATTTTTTTTATTTTCAAA‑TTTTTTAAAAATTTATTTTAAATTTTAAAAAATA‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAtttt                                                                                 <  1:948374/128‑1 (MQ=255)
                                             tgAAACATAAACTTTTATATTTTTAGT‑ATTTTTTTTTATTTTGAAA‑TTTTTAAATAATTAATTTTTAATTGTTAAAAAT‑‑‑TTGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                                                <  1:1225313/127‑1 (MQ=255)
                                             ataaaaaaaaaataTTTTATTTTTATA‑TATTTTTT‑TAAATTTAAATTTTTTATAAAAATTATTTTAAATTGTAAAAAATA‑‑‑ATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                                                <  1:895724/121‑1 (MQ=255)
                                              taaaataataataTTATTTTTTTTTA‑TATTTTTTGTATATTAAAA‑TTTTTTAAAAATTAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTttg                                                                                <  2:552592/127‑1 (MQ=255)
                                               aaactaaaactaTTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAT‑‑TGTAAATATAAATTTTAGTTATTttgtt                                                                              >  1:2479929/1‑128 (MQ=255)
                                                   taaaaaatttattttttttta‑tttttttTTTTTATTTAAA‑TTTTTTAAAAATTAATTTTATCTTTTAAAAAATA‑‑‑‑TATTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTa                                                                         <  2:3038059/120‑1 (MQ=255)
                                                    taaacatatttttttttATT‑TATTTTTTTTTTATTTAAATTTTTAAAAAAATAAATTTTAA‑TTTTAAAAAATAG‑‑‑GTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTCGTTGTCTa                                                                         <  1:1347285/116‑1 (MQ=255)
                                                    aaaaataTTATTTTTTTTTT‑AATTTTTTTTTAATGAAAA‑TTTTTAAAAAATAAATTTTAATTTTTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                                                       <  2:2881633/128‑1 (MQ=255)
                                                     aaattaTTATTTTTTTTTT‑TTTTTTTTGTATATTTAAA‑TTTTTTAAAAATTAATTTTTACATGTTAAAAA‑‑‑‑TTGGTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                                                       <  1:2016412/128‑1 (MQ=255)
                                                      aaaattttattttttATATTATTTTTTTTATATTCAAA‑TTTTTTAAAAATTAATTTTTAATTTTAAAAAATTGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAg                                                                       <  1:527494/118‑1 (MQ=255)
                                                       actaTTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAAATTAAATTTTATGTATTTttttgttaaag                                                                       >  1:290114/1‑123 (MQ=255)
                                                       aattttattttttttta‑tttttttTGTTTATTCAAA‑TTTTTTAAAAATTTATTTTAATTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTa                                                                     <  1:1553664/124‑1 (MQ=255)
                                                         tattatCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTATTTATTTTTTTTTATaatttt                                                                    >  2:1818556/1‑124 (MQ=255)
                                                          atttttttttttta‑ttttttttttatttTTAAA‑TTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCt                                                                  <  1:473077/122‑1 (MQ=255)
                                                          attatCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAATTTTTATTTATTTTTTTTTATaattttat                                                                  >  2:2789110/1‑122 (MQ=255)
                                                           ttatCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTAATAATTAAAATATAAATTTTAGTTATtttttttaataagaaaat                                                                  >  1:718797/1‑116 (MQ=255)
                                                            tatTTTTTTTTA‑TATTTTTTTTTTAATTAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaa                                                                <  1:2860667/128‑1 (MQ=255)
                                                            tatCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATATAAATTTTATTTATTTTTATGTATaataaacaaa                                                                >  1:1977739/1‑120 (MQ=255)
                                                               cTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATaaaaaaaa                                                            >  2:3096888/1‑128 (MQ=255)
                                                                tttttATA‑TATTTTTTTTTTATTAAAA‑TTTTTAAAAAATTAATTTTAAATTTTTAAAAATA‑‑‑‑‑TCTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTaaaaaaa                                                           <  1:3096888/128‑1 (MQ=255)
                                                                 ttttAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTAAAATGAAAATAAAAATTTAAGTAATTTTTTTGTATAATTAAATaaaaaaa                                                           >  2:2025335/1‑128 (MQ=255)
                                                                  tttAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTAAAATTTAAATATAAAATTTAAGTTTTTATTTGAGTAATTTTCTAAAAAAAt                                                          >  1:2535980/1‑128 (MQ=255)
                                                                  tttAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTAGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTAATAATTGAAATATAAATTTTAGATAATTTGTTGTATAAATATATaaaaataa                                                          >  1:1443285/1‑125 (MQ=255)
                                                                   tttattttttatttGTATATTCAAA‑ATTATATAAATTTATTTTTCTCTTGTAAAAAATTAC‑‑‑TTTTTTTTTTTTTTTTATAATGGAATTATAAATTTTAGTTATTTTGTTGTCTAAGTATCTaaaaaaa                                                           <  1:2376972/117‑1 (MQ=255)
                                                                   ttAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTT‑‑‑‑‑TAATGTAAATAAAAAATTTATTTATTTTTTTTTATAATTATATAAAAAAATat                                                        >  1:1725977/1‑127 (MQ=255)
                                                                    tAGA‑TATTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATAAAAATTTTATTTATTTTTTTTTATAATTTTATAAAAAAATat                                                        >  2:1141539/1‑127 (MQ=255)
                                                                       tttatTTTTTTTATTTTTAAA‑TTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAg                                                        <  2:3194132/126‑1 (MQ=255)
                                                                          tttttttttttATTAAAA‑TTTTTAAAAAATTAATTTTTATTTTTAAAAAA‑A‑‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAATTTTTTGTTTTTTTTTTGTTTAATTATATAAAAAATTATaatga                                                   <  1:163382/127‑4 (MQ=255)
                                                                          atTTTTTGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTAATAATTATATAAAAAAATAaaaatta                                                  >  1:3189947/1‑125 (MQ=255)
                                                                             ttttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTATTTATTTTTTTTTATAATTTTATAAAAAAAAATAAAGaaaa                                                >  1:2225253/1‑128 (MQ=255)
                                                                              tttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAATAAAAATTaaaa                                               >  2:415079/1‑128 (MQ=255)
                                                                               ttttataTTTAAA‑TTTTTAAAAAATTTATTTTAAATTTTAAAAAATA‑‑‑TATTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                                               <  2:2226542/125‑1 (MQ=255)
                                                                               ttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTTTTTTTTTTTATAATTATATAAAAAAATATaaattaaa                                                >  2:2352823/1‑123 (MQ=255)
                                                                               ttGTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTTTTTTTTTGTCTAATTTAAAAAAAAAATAAAAAGGaaaa                                               >  1:2683794/1‑128 (MQ=255)
                                                                                 gTATATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTT‑‑‑TAATGTAAATATAAATTTTAGGTATTTTGTTGATAAATTATTAAAAAAAATTAAAAGTaaaaaa                                             >  1:3211547/1‑128 (MQ=255)
                                                                                   ttttttaaa‑TTTTTAAAAAATTAATTTTAATTTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAata                                           <  2:2100440/122‑1 (MQ=255)
                                                                                   ttatTCAAA‑TTTTTTAAAAATTTATTTTAAATTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                                            <  2:678688/127‑1 (MQ=255)
                                                                                      tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTT‑‑‑‑TAATGGAATTATAAATTTTATTTATTTTTTTTTATAATTTTCTAAAAAAATAAAAAGTAAAAAATAtttt                                       >  1:2155526/1‑126 (MQ=255)
                                                                                      tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATGTATTTTTTTTTCTAATTTAATAAAAAAATATAAATAaaaaaaattttt                                       >  2:644359/1‑122 (MQ=255)
                                                                                      tGCAAA‑TTTTTAAAAAATTAATTTGAACTAGAAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTAAAATTAAAAATTTAGGTTTTTTGTTGTCTAATTATCTAAAAAAATAGAAATGAAAAAATAtttt                                       >  1:2653311/1‑126 (MQ=255)
                                                                                       gCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATTTAAATTAAAATTTTAGGTATTTTTTTGTATAATTATATAAAAAATTAGAAAGGAAAAAattatttt                                      >  2:1730597/1‑122 (MQ=255)
                                                                                                 taaaaaaTTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTAAT‑‑TGTAAATATAATTTTTTGTTTTTTTTTTTTATAAGTAAAAAAAAAAATAAAAATAAAAAAATTTTTTTaaattta                               >  2:2535949/2‑124 (MQ=255)
                                                                                                 taaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATTTAAATTTTATTTATTTTTTTTTATAATTATATAAAAAAATTAAAAGTAAAAAATATTTTTAAAATTaa                              >  2:1248356/2‑128 (MQ=255)
                                                                                                    aaaattaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTA‑ATTGAAAATTAAAATTTTTGTTTTTTTTTTTTAAAATTTAATAAAAAAAAAAAATGAAAAAAATATT‑TTAAAATTAATaaa                          >  1:171267/3‑126 (MQ=255)
                                                                                                     aaattaattTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAATTATAAATTTTATGTTTTTTTTTGTATATTTATGTAAAAAAATATAAATGAAAAAAAAATGTTAAAAGTataaatta                        >  1:2355838/2‑121 (MQ=255)
                                                                                                      aattaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTT‑‑‑‑‑TAATGTAAATATAAATTTTAGTTATTTTTTTTTCTAAGTAAATAAAAAAATATAAAGAAAAAAATATTTTTAAAATAAATAATaaa                       >  1:2799040/1‑128 (MQ=255)
                                                                                                      aattaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTAATAATGTAAATAAAAATTTAAGTTATTTTGTTTTATAATTATAAAAAAAATAAAAAAGTAAAAAATATTTTTAAAATAAataata                         >  2:1555296/1‑128 (MQ=255)
                                                                                                      aattaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATTATGTAAATAAAAATTTTTGTTATTTTGTTGTAAAATTATAAAAAAAAATAAAAATTAAAAAATTTTGTTAAAATTAAAACaaa                        >  2:2981609/1‑128 (MQ=255)
                                                                                                      aattaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATATTGAAAATAAAAATTTTAGTTATTTTTTTGTATAAGTATCTAAAAAATTTAAAAGGAATAAATATTGTTTAATTTAATaaaaa                        >  1:647515/1‑124 (MQ=255)
                                                                                                       attaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTTTTGTATAAGTATATAAAAAAATATAAATGAAAAAATATTGTTAAAATTAATACTa                         >  2:1069838/1‑128 (MQ=255)
                                                                                                       attaattTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTTGGTATTTTGTTTTATAATTATATAAAAAATTATAAATGAAAAAATATTGTTAAATTtaataataa                        >  2:1889605/1‑128 (MQ=255)
                                                                                                       attaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATTAAAATTTAAGTTATTTTTTTGACAATATAACTAAAAAAAAAAAAAGTAAAAAATATTATaaaatatataaataaa                       >  1:2039925/1‑116 (MQ=255)
                                                                                                        ttaattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTT‑‑‑‑‑TAATTGAAATTTAAATTTTTTGTTATTTTTTTTATAATTTTATTAAAAAATTAAAATGAAAAAATTTTGTTAAAAAGAaaaataaaat                     >  2:2837459/1‑128 (MQ=255)
                                                                                                        ttaattTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTA‑AATGGAAATTTAATTTTAAGGTATTTTTTTTAATATGTATATAAAAAAATTTAATGGAAAAAATTTTTTTAAAAGTAATATTaaa                       >  2:886141/1‑128 (MQ=255)
                                                                                                        ttaattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGTAAATATAATTTTTAGTTTTTTTTTTTTATAATTATATAAAAAAATATAAAGTAAAAAATATTGTTAAATTTAATACTaaaa                      >  2:1526207/1‑128 (MQ=255)
                                                                                                        ttaattTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTATTTTTTTTTCTAATTTTATAAAAAAATATAAATTAAAAAATATTGTTAAAATTAATAATAAAat                     >  2:1197892/1‑128 (MQ=255)
                                                                                                        ttaattTGAACTTGTAAAAAATAGC‑‑‑‑‑TTTTTTTTTTTTTAATAATGAAAATAAAAATTTTAGGATATTTGTTGTATAAAATACTAAAAAAATATAAAGAAAAAAAAATTATTAAAATAAACACTAAAat                     >  1:1620691/1‑128 (MQ=255)
                                                                                                         taattTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATAATGGAAATTTAAATTTTAGGTTTTTTGTTGTATAATTTAATAAAAAAATATAAAGTAAAAAATTTTTTTAAATTTAATAGTAatat                     >  1:1252687/1‑128 (MQ=255)
                                                                                                          aattTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTT‑‑‑‑‑TAATTGAAATTTAAATTTTAGGTTTTTTTTTTTATAATTATATAAAAAAATATAAATGAAAAAATATTGTTATAAATTATAATAAAatta                   >  2:820463/1‑128 (MQ=255)
                                                                                                             ttGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTTATATTGGAAATATAAATTTTATGTATTTTGTTGTATAATTTTATAAAAAAATATAAAGTAAAAAATTTTGTTAAATTAAATAATAAAtttatt                 >  2:970369/1‑128 (MQ=255)
                                                                                                               gAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTAAAAATGTAATATTAAATTTTAGTTTTTTTTTTTTATATTTATAAAAAAAATTATAAATTAAAAAAATTTTTTAAAATTAATAAAAAAtttattta               >  1:2828129/1‑128 (MQ=255)
                                                                                                                             aaaatttttttttttttttttttATAATTGAAATATAAATTTTAGGTATTTTGTTGTATAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGttaatta     <  2:1743969/124‑1 (MQ=255)
                                                                                                                                 |                                                                                                                              
ATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGA‑TATTTTTTGTATATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATACTAAAATTATTTAATGTTAATTAAAA  >  CP016816/165123‑165376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑