BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	165,222	G→A	intergenic (+186/‑356)	JCVSYN2_00665 → / → JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	165,222	0	G	A	98.6%	4441.2 / 39.0	1457	intergenic (+186/‑356)	JCVSYN2_00665/JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (955/475); minor base . (5/15); total (962/495)
Fisher's exact test for biased strand distribution p-value = 1.80e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 1495 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAA  >  CP016816/165097‑165348

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAaat                                                                                                                              <  2:912481/128‑1 (MQ=255)

  ttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTATTAAAAaatt                                                                                                                             >  1:385578/1‑128 (MQ=255)

   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAaatta                                                                                                                            >  1:4328448/1‑128 (MQ=255)

     aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAaattaat                                                                                                                          >  1:3074451/1‑128 (MQ=255)

     aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAaattaat                                                                                                                          >  1:1518021/1‑128 (MQ=255)

       tGTATATCTTTACTATTTGATAATATTTAGTTTCATTAATTTATAGCACTTGTATTTATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAAttt                                                                                                                        <  2:422673/128‑1 (MQ=255)

         tATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGa                                                                                                                      <  2:1380778/128‑1 (MQ=255)

            tactttactAGTTTATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACt                                                                                                                   <  2:665812/128‑1 (MQ=255)

            tactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACt                                                                                                                   >  1:3962869/1‑128 (MQ=255)

               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTTAACTTGt                                                                                                                >  1:3424089/1‑128 (MQ=255)

               tttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGt                                                                                                                >  1:318486/1‑128 (MQ=255)

                 tactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                              >  2:3031705/1‑128 (MQ=255)

                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                             <  2:1128366/128‑1 (MQ=255)

                  actAGTTGATAATATTTAGTTTCATTAATTTATAGAACTTGTATGAATTAGTAAGAAACTAAAACTAATATCTTATTAGATATATATTTTA‑TATGCAAA‑ATTTTAAAAAATTAATTATAACTATTaaa                                                                                                             >  2:1513151/1‑128 (MQ=255)

                    tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                           >  2:4137471/1‑128 (MQ=255)

                    tAGTTGATAATATTTAGTTTCATTAATTTATAGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                           <  2:787499/128‑1 (MQ=255)

                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                        >  2:4454728/1‑128 (MQ=255)

                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                        >  1:1095017/1‑128 (MQ=255)

                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                        >  1:2217542/1‑128 (MQ=255)

                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTATAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATATGAACTAGTAAAAAATAGC‑tt                                                                                                   >  2:3554039/1‑128 (MQ=255)

                               atTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttt                                                                                               >  2:3222606/1‑128 (MQ=255)

                                 ttAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttttt                                                                                             >  1:594321/1‑128 (MQ=255)

                                  tAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGATACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGA‑tttttttat                                                                                            >  2:2971713/1‑126 (MQ=255)

                                   aGTTTCATTAATTTATCGCACTTGTATTAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTATTTGTA‑TATGCAAA‑TATTTAAAAATTTAATTTGAACTAGAAATAAATAGC‑ttttatattt                                                                                           >  1:1764133/1‑128 (MQ=255)

                                    gTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttt                                                                                          >  1:127218/1‑128 (MQ=255)

                                    gTTTCATTAATTTATCGCACTTGCATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttt                                                                                          >  1:488324/1‑128 (MQ=255)

                                       tCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑tttttttttttttt                                                                                       >  2:3600894/1‑128 (MQ=255)

                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttt                                                                                      >  2:430406/1‑128 (MQ=255)

                                        cATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttt                                                                                      >  2:2432174/1‑128 (MQ=255)

                                             aTTTAACCCACTTTTATGAATTACTAATAAACAAAAAAAAATATTATTTTATTTATTTTTTTTA‑TATTGAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATACC‑TTTTTTTTTTTTTTTTTTta                                                                                 <  2:346202/128‑1 (MQ=255)

                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑ttttttttttttttttttttt                                                                                >  2:3934070/1‑126 (MQ=255)

                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTtat                                                                                >  1:4568339/1‑128 (MQ=255)

                                               ttATCGCACTTTTATTTATTATTAATTAACTAAAACTATTATTTTTTTAGATATTTTTTGTT‑TATTCAAA‑TTTTTAAAAAATTAATTTTAAATTGTAAAAAATAGC‑tttttttttttttttataatgg                                                                               <  2:3727700/128‑8 (MQ=255)

                                                  actgaaatttATTAATTAGTAAAAAAATAAAACTAATATTATTTTTTATATTTTTTTTATTATCAAAA‑TTTTAAAA‑AATTAAATTTAAATTGTAAAAAAATGC‑‑TTTTTTTTTTTTTTTTTTATAATgg                                                                           <  1:1369456/119‑1 (MQ=255)

                                                   cGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATATTga                                                                           >  2:2479447/1‑127 (MQ=255)

                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTAtattataaataaa                                                                      >  2:560334/1‑121 (MQ=255)

                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGAAAA‑TTTTTAAAAAATTAATTTTAACTTGTAAAAAATATC‑TTTTTTTTTTTTTTTTTTtatatattaaaat                                                                      >  2:1458025/1‑119 (MQ=255)

                                                         tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAAtata                                                                    >  2:2614662/1‑128 (MQ=255)

                                                         tGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAAtata                                                                    >  2:1600838/1‑128 (MQ=255)

                                                             ttatttattaATAAAATTATACTATTATTTTTTTTTTTTTTTTATGTA‑TTTTCAAA‑TTTTTAATAAAATAATTTTAAATATTAAAAAATA‑T‑GTTTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                <  2:2148404/124‑1 (MQ=255)

                                                              gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAAT‑TAAAtttt                                                              >  1:2802862/1‑128 (MQ=255)

                                                               aaTTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTAT‑TATTGTAATTATAAAtttt                                                              >  2:1923261/1‑128 (MQ=255)

                                                                attattAATAAAATAAAAAATTTATCTTTTTTTTTTTTTTTTTTA‑TATTAAAT‑TTTTTTAAAAATTAATTTTAAATTTTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTa                                                             <  2:2532745/128‑1 (MQ=255)

                                                                 ttattaATAAAATAAAAATATTATCTTTTTATATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTTTAAAAAATAGC‑‑‑‑‑‑TTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                        <  2:2799976/128‑1 (MQ=255)

                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTCAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTAAgtt                                                          >  1:2119184/1‑126 (MQ=255)

                                                                   attattaaaataaaaattATATCTTTTTTTTTTTTTTTTTTA‑TATTAAAA‑TTTTTAAAAAATTATTTTTAATTTTTAAAAAA‑ATC‑CTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                          <  1:422430/122‑1 (MQ=255)

                                                                   attaataaAATAAAAATTTTTTATTTTTATTAATTTTTTTTA‑TATGAAAA‑TTTTTAAAAAATTAATTTTAACTTTTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAgg                                                           <  2:3131511/126‑1 (MQ=255)

                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAgtt                                                          >  2:4534483/1‑126 (MQ=255)

                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATATAAAAAAAAAtataatat                                                          >  1:2654089/1‑121 (MQ=255)

                                                                    gTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATTTAAATA‑AAATTTTatttt                                                         >  2:3804770/1‑124 (MQ=255)

                                                                     taataaAATTAAAATTTTATTTTTTTTTATTTTTTTTTTT‑TATTTAAATTTTTAAAAAAATTATTTTTAA‑TTTTTAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTAt                                                        <  1:4492000/128‑1 (MQ=255)

                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGATCTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TATTGGAATTATAAATTTTTTTTATTttg                                                    >  1:3663044/1‑128 (MQ=255)

                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTATttttttt                                                 >  2:220617/1‑127 (MQ=255)

                                                                           aaaaaaaataTTATTTTTTTATTTTTTTTTTTTT‑TTTTTAAA‑TTTTTAAAAAATTAATTTTAAATTGTTAAAAAAA‑‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTtgttgt                                                <  1:1993588/125‑1 (MQ=255)

                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTA‑AATTTAAAAATAAATTTTAGTTATtttttttt                                                >  1:1156128/1‑126 (MQ=255)

                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTTAATAAAAATTTTAGTTATtttttttt                                                >  2:1294950/1‑126 (MQ=255)

                                                                                aactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTT‑‑‑TAATGTAAATATAAATTTTAGTTATTTTGTTGTCTaat                                           >  1:3488848/1‑127 (MQ=255)

                                                                                aactaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTAGGTATTTTGTTGTATaa                                            >  2:2351881/1‑128 (MQ=255)

                                                                                 actaTTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑‑TTTTTTTTTTTTTTATATAATGGAAATATAATTTTTAGGTATTTTTTTTTCTAAGt                                          >  1:404881/1‑128 (MQ=255)

                                                                                    attatCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAAAT‑GAAATAAAAATTTTAGTTATtttttttaaaaatta                                         >  1:3301283/1‑119 (MQ=255)

                                                                                    attatCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATTTTTTTTTATAATTa                                         >  2:3314774/1‑128 (MQ=255)

                                                                                    attatCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAG‑‑ATTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTATtttttttaataatta                                         >  2:2709354/1‑119 (MQ=255)

                                                                                      tatCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAATTATTTTTTTTTATaatttta                                       >  2:1988164/1‑123 (MQ=255)

                                                                                      tatCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTATTTATTTTTTTTTATAATTAtat                                      >  2:1212521/1‑126 (MQ=255)

                                                                                           ttttAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATTGAATTATAAATTTTTTTTTTTTTTTTGTAAAATTATATaaa                                   >  1:2884014/1‑128 (MQ=255)

                                                                                           ttttAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAATTATAAATTTTAGTTATTTTTTTTTATAATTAAATaaaa                                  >  1:2069627/1‑128 (MQ=255)

                                                                                           ttttAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAAAATAAATTTTAGGTTTTTTTTTGTCTAAGTATCTaaaaa                                 >  1:2489831/1‑128 (MQ=255)

                                                                                            tttAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑CTTTTTTTTTTTTTTTTATAATTGAAATTAAAATTTTATTAATTTTTTTTTATAATTATATaaaaaa                                >  2:855990/1‑128 (MQ=255)

                                                                                              tAGATATTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TAATTTAAATATAAATTTTTGTTATTTTTTTTTATAATTATATaaaaaaa                               >  1:1623759/1‑128 (MQ=255)

                                                                                                 atatTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTATA‑AATGTAAAAAAAAATTATAGTTATTTTTTTTTAAAATTATAaaaaaaaaaaa                            >  1:876397/1‑125 (MQ=255)

                                                                                                  tatTTTTTTTT‑TATTAAAA‑TTTTTAAAAAATTTATTTTAAATTTTAAAAAA‑ATT‑GTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGa                           <  1:2972492/128‑1 (MQ=255)

                                                                                                  tatTTTTTGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTAGGTTTTTTTTTGATTAATTATCTAAAAAAATATaa                          >  1:4139393/1‑128 (MQ=255)

                                                                                                    ttttttGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGTTATTTTTTTTTATAATTTTATAAAAAAATATaaa                         >  2:185519/1‑128 (MQ=255)

                                                                                                     tttttGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTAAAATTTAAATAAAAATTTAAGTTATTTTTTTTTATAATTATATAAAAAAATATaaat                        >  2:4012609/1‑127 (MQ=255)

                                                                                                         ttttttatGCAAA‑ATTTTAAAAAATTAATTTTTAATTTTAAAAAAATGC‑‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                   <  1:3600894/123‑1 (MQ=255)

                                                                                                         tGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATATATTTTTGTTTTTTTGTTTGATAAGAATATAAAAAAATTGAAAgtat                     >  1:533104/1‑125 (MQ=255)

                                                                                                         tGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTAAAATAAAATTTTTAGTTTTTTTTTTTTATATTTATAAAAAAAAAAAAAAAGTaaa                    >  1:1003325/1‑128 (MQ=255)

                                                                                                         tGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGTAAAATAAAATTTTAGTTATTTTGTTGACAAATTATATAAAAAAAAATAAAGaaaa                    >  1:4416594/1‑128 (MQ=255)

                                                                                                         tGTA‑TATGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATGTAAAAAAAATTTTTATTTTTTTTTTTGTAAAATTATATAAAAAAATATAAAGTaaaa                   >  2:1773449/1‑128 (MQ=255)

                                                                                                          ttttatgataaa‑TTTTAAAA‑AATTTTTTTGTATTTTTAAAAAAT‑TT‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                   <  2:365666/119‑1 (MQ=255)

                                                                                                           tttattttaaa‑TTTTTTAAAAATTAATTTTAAAATTTAAAAAAATTT‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaa                    <  2:2064815/120‑1 (MQ=255)

                                                                                                           ggttattaaaa‑TTTTTAAAAAATTAATTTTTACTTTTTAAAAATA‑T‑GTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaa                   <  1:2423707/120‑1 (MQ=255)

                                                                                                            a‑tatGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGTTATTTTTTTTTATAAGTATATAAAAAAATAAAAATTaaaaaa                 >  2:1667988/1‑128 (MQ=255)

                                                                                                             ttatTCAAA‑TTTTTTAAAAATTAATTTTAAATTTTAAAAAATTTC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGaaaaaa                 <  2:491158/127‑1 (MQ=255)

                                                                                                              tatGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TATTGTAATTAAAAATTTTA‑GTATTTTTTTTTTTAAATTTAAAAAAAAATTAAAATGtaaaaaat               >  1:2306737/1‑126 (MQ=255)

                                                                                                              tatGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAATTATATAAAAAAATATAAAGGAAAAAat                >  2:3402881/1‑128 (MQ=255)

                                                                                                              tatGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGAAAATATAAATTTTATGATTTTTGTTGTATAATTATAAAAAAAAATATAAATAAAAAAat                >  2:4398529/1‑128 (MQ=255)

                                                                                                              atttaaaatttttTAAAAAATTTATTT‑AATTTTTAAAAAAT‑TA‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAat                <  2:4080602/119‑1 (MQ=255)

                                                                                                               attaaaa‑TTTTTTAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGCATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAatat              <  1:1886184/124‑1 (MQ=255)

                                                                                                                tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTTTTAATGGAAATATAAATTTTTGGTATTTTTTTTTATAATTTAATAAAAAAATAAAAAGGAAAAAat                >  1:3793495/1‑128 (MQ=255)

                                                                                                                tGCAAA‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATTTAAAAATAAATTTTAGTTTTTTTTTTTTATAATTAAAAAAAAAAATATAAATTAAAAAata               >  1:754503/1‑128 (MQ=255)

                                                                                                                   aaa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTT‑TATTTGAAAAAAAAATTTTAGTTTTTTTTTTTTAATAGTATATAAATAATTTTAAAGAaaaaaaattttt           >  1:2596187/1‑122 (MQ=255)

                                                                                                                   aaa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTCTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTg            <  1:1431135/128‑1 (MQ=255)

                                                                                                                   aaa‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTTTTTTTTTGAAAAAGTATCTAAAAAAAAAAAAAGGAAAAAAAAtttt           >  1:957165/1‑126 (MQ=255)

                                                                                                                     a‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATATC‑‑TTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTa         <  2:3863025/128‑1 (MQ=255)

                                                                                                                     a‑TTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTAGTTATTTTGTTTTATAATTATGTAATAAAATAGAAAGTAAAAATTTATGTTaa        >  2:2260902/1‑128 (MQ=255)

                                                                                                                        tttAAAA‑AATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTTAAAATTAAAAAATAAATTTTAGTTATTTTTTTTTATAATTATAAAAAAAAAAAAAAATAAAAAAAAATTTTTaaaat     >  1:1678906/1‑127 (MQ=255)

                                                                                                                          ttaaaaaaTTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTTTAATGGTATAATAAATTTTAGTTTTTTTTTTTTTTAAGTATATAAAAAAATATAAAGAAAAAATTATTTTTaaat      >  2:2889637/3‑127 (MQ=255)

                                                                                                                           taaaaaaTTTATTTTAAATTGTTAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTa   <  2:1033885/127‑1 (MQ=255)

                                                                                                                            aaaaaaTTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTAAAATGGAAATAAAAATTTTTGTTATTTTGTTGTAAAAGTAAATAAAAAAATAAAAATGAAAAAATTTTTTTaaaatt    >  1:3728363/1‑126 (MQ=255)

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTA‑TATGCAAA‑TTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTATTTTGTTGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAA  >  CP016816/165097‑165348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`