BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	420,157	2 bp→TT	intergenic (‑440/+402)	JCVSYN2_01895 ← / ← JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,157	0	A	T	99.3%	8442.0 / NA	2686	intergenic (‑440/+403)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (7/11); new base T (1439/1225); total (1448/1238)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP016816	420,158	0	A	T	98.0%	8062.1 / ‑4.3	2686	intergenic (‑441/+402)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base A (9/46); new base T (1438/1192); total (1448/1238)
Fisher's exact test for biased strand distribution p-value = 9.45e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2777 total aligned reads displayed.

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285

||

       attcattcATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGATAACTAATAGCACTTTTTTAAAAAATttttaatttta                                                                                                                            >  1:1801297/1‑125 (MQ=255)

        ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGGTATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                           <  2:5813616/128‑5 (MQ=255)

        ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                           <  2:1886686/128‑5 (MQ=255)

        ttcattcaTACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATttttaattttaa                                                                                                                           <  1:3006114/128‑5 (MQ=255)

          cattcatACTTTTAGTCTCATAAATCTTAGTTTCTAAAATTATTTTATCATTTAAAATAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                         <  2:2841308/128‑1 (MQ=255)

          cattcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATa                                                                                                                         <  1:3208556/128‑1 (MQ=255)

           attcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTtaataa                                                                                                                        <  1:4707141/128‑1 (MQ=255)

            ttcatACTTTTAGTCTCTTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                       >  1:6524077/1‑128 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                       <  2:4962444/128‑1 (MQ=255)

            ttcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAc                                                                                                                       <  1:5482144/128‑1 (MQ=255)

             tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                      <  1:2338788/128‑1 (MQ=255)

             tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                      <  1:2731725/128‑1 (MQ=255)

             tcatACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACt                                                                                                                      <  1:934923/128‑1 (MQ=255)

               atACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGa                                                                                                                    <  2:6449093/128‑1 (MQ=255)

                tACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGaa                                                                                                                   >  1:1580991/1‑128 (MQ=255)

                 aCTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTATCACTTTTTTAAAAAATTTATAATTTTAATAAATGAat                                                                                                                  >  1:2837737/1‑128 (MQ=255)

                  cTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatt                                                                                                                 <  1:2067658/128‑1 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                >  1:3890730/1‑128 (MQ=255)

                   ttttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATAATTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                <  1:5356890/128‑1 (MQ=255)

                   ttttAGTATCATAAATATAAGTTTCTAATATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAatta                                                                                                                <  1:4838504/128‑1 (MQ=255)

                    tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattat                                                                                                               >  1:3099875/1‑128 (MQ=255)

                    tttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAAAAATACTTTTATTAGTTGATATATTAGAAAAATACTAGCACTTTTTTAAAAAATATTTAATTTTAATAAAAGAattat                                                                                                               >  1:797059/1‑128 (MQ=255)

                     ttAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatt                                                                                                              >  2:3668411/1‑128 (MQ=255)

                      tAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAattatta                                                                                                             >  1:1899532/1‑127 (MQ=255)

                       aGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGATAACTACTATCACTATTTTAAAAAATTTTTAATTTTAATAACTGAATTAtttt                                                                                                            >  1:2555661/1‑128 (MQ=255)

                           tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                        >  2:772094/1‑128 (MQ=255)

                           tcATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCaa                                                                                                        <  2:5165688/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                      >  2:4283297/1‑128 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                      <  2:4596243/128‑1 (MQ=255)

                             aTAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                      >  2:3121150/1‑128 (MQ=255)

                             aTAAATCTAAGTTTATAAACTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAt                                                                                                      <  2:5251795/128‑1 (MQ=255)

                              tAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAAtt                                                                                                     <  1:5678761/128‑1 (MQ=255)

                               aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTTAATTATTTTTCAAATTg                                                                                                    >  2:3918483/1‑128 (MQ=255)

                               aaaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTg                                                                                                    >  2:2196897/1‑128 (MQ=255)

                                aaTCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGt                                                                                                   <  1:711262/128‑1 (MQ=255)

                                      aGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAa‑tt                                                                                            >  1:674782/1‑128 (MQ=255)

                                       gTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAa‑tta                                                                                           >  2:2613367/1‑128 (MQ=255)

                                        tttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAa‑ttat                                                                                          <  1:5771805/128‑1 (MQ=255)

                                        gttCTAAAATTATTTTATAATTTATAAAAATAATACTTTTATTAGTTGATATATTAGATAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAa‑ttat                                                                                          <  2:6714610/127‑1 (MQ=255)

                                         ttCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAa‑ttatt                                                                                         >  1:4438279/1‑128 (MQ=255)

                                          tCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTAttt                                                                                        <  1:5990732/128‑1 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTAtttt                                                                                       >  2:6186513/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTAtttt                                                                                       >  1:3686057/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTAtttt                                                                                       >  2:4095934/1‑128 (MQ=255)

                                           cTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTAtttt                                                                                       >  1:5585873/1‑128 (MQ=255)

                                             aaaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCa                                                                                     >  2:2480749/1‑128 (MQ=255)

                                              aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCaa                                                                                    >  1:1463089/1‑128 (MQ=255)

                                              aaaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCaa                                                                                    <  1:6610944/128‑1 (MQ=255)

                                               aaTTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCaaa                                                                                   <  2:2018901/128‑1 (MQ=255)

                                                  tattTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCa                                                                                >  2:5379954/1‑128 (MQ=255)

                                                   attTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCAt                                                                               >  2:5711999/1‑128 (MQ=255)

                                                     tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑a                                                                            <  1:6186243/128‑1 (MQ=255)

                                                     tttATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAAATGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑a                                                                            >  1:4011148/1‑128 (MQ=255)

                                                       tATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATc                                                                          <  1:4984845/128‑1 (MQ=255)

                                                        aTCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTATTAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCt                                                                         <  1:3337100/128‑1 (MQ=255)

                                                         tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTa                                                                        <  1:5087637/128‑1 (MQ=255)

                                                         tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTa                                                                        <  2:5081172/128‑1 (MQ=255)

                                                         tCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTa                                                                        >  2:1802013/1‑128 (MQ=255)

                                                          cATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTAAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTAt                                                                       >  2:1362331/1‑128 (MQ=255)

                                                                aaaaaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAAtat                                                                 >  1:1332368/1‑126 (MQ=255)

                                                                   aaaTAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATATTAATAACTGAATTATTTTTCAAATTGTTAAT‑TTATTTTCAAATCATT‑ATCTATTAATCTAtt                                                              >  2:6029509/1‑128 (MQ=255)

                                                                    tataataCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAAttt                                                             <  1:196816/127‑1 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAAttt                                                             >  1:2459464/1‑128 (MQ=255)

                                                                    aataatACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAAttt                                                             <  2:5496925/128‑1 (MQ=255)

                                                                         taCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCt                                                                         <  2:2995943/111‑1 (MQ=255)

                                                                          aCTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCt                                                       >  2:843946/1‑128 (MQ=255)

                                                                             tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGt                                                    >  1:6300183/1‑128 (MQ=255)

                                                                              tttttaTTTTATATATTAGAAAAATACTATCACTTTTTTAAAAAATTTTTAATTTTAATAAATTAATTATTTTTTAAATTGTTATA‑TTATTTTAAAATAATT‑ATCAATTAATCAATTTTTTCCTCGtt                                                   <  2:3503424/125‑1 (MQ=255)

                                                                               tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGttt                                                  >  2:592/1‑128 (MQ=255)

                                                                                       ttatatttATAAAAATAATATCAATTTTTTAAAAAATTTTTATTTTTAATAACTTATTTATTTTTAAATTTGTTAAA‑TTATTTTAAAATAATT‑ATATATTAATCAATTTTATGCTCGttttttttttt                                          <  2:1539350/122‑1 (MQ=255)

                                                                                         tatatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGttttttttttttt                                        >  1:2172878/1‑128 (MQ=255)

                                                                                          atatTAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGtttttttttttttt                                       >  2:6321722/1‑128 (MQ=255)

                                                                                              tAGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGttttttttttttttttaa                                   >  1:3559616/1‑126 (MQ=255)

                                                                                               aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAAt                                                                                  >  2:1171179/1‑81 (MQ=255)

                                                                                               aGAAAACTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCTttttttttttttttttaaa                                  >  1:2268672/1‑125 (MQ=255)

                                                                                                  aaaaTACAAACAATTTTTTAAAAAAATTTTAATTTTAAAAAATTATATATTTTTTAAAATTTTAAA‑TTTTTTTAAAAATATT‑AAATATTAATTAATTTTATTCTCGTTTTTTTTTTTTTTTTTACaaa                               <  1:5869966/128‑1 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAaaaa                               >  1:1230088/1‑128 (MQ=255)

                                                                                                  aaaCTACTAGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACaaa                               >  1:3788770/1‑128 (MQ=255)

                                                                                                    ataaaattaatttttttAAAAAATTTTAATTTTAAAAAAAT‑AAATATTTTTTAAAATTTTAAA‑TTTTTTTAAAAAAATT‑AAAAATTAAAAAATTTTATTCCCGTTTTTTTTTTTTTTTTTACAAAAat                            <  1:4617729/117‑1 (MQ=255)

                                                                                                     cttctaGAACTTTTTTAAAAAATTTTTATTTTTAATAACTTTATTATTTTTCAAATTTTTAAA‑TTATAATCAAATCATA‑ATCTATTACTCAATTTTATGGGCGTTTTTTTTTTTTTTTTTACAAAAat                            <  2:3785184/125‑1 (MQ=255)

                                                                                                     ctactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTTTaaaaaa                            >  2:6598234/1‑127 (MQ=255)

                                                                                                      tactaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACaaaaaat                           >  2:559482/1‑126 (MQ=255)

                                                                                                        ctaGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGATCG‑TTTTTTTTTTTTTTTTACAAAAATATaa                        >  1:5187543/1‑128 (MQ=255)

                                                                                                         tattaattttttAAAAAATTTTTAATTTTAATAAATTAATTATTTTTAAAATTTTTAAA‑TTATTTTAAAATAATT‑TTTTATTAATAAATTTTTTGTTCGTTTTTTTTTTTTTTTTTACAAAAATATaa                        <  2:3367638/122‑1 (MQ=255)

                                                                                                         taGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATaaaa                        >  1:4117042/1‑128 (MQ=255)

                                                                                                          agaaattttttAAAAAATTTATAATTTTAAATATTTAATTAATTTTCTAATTTTTAAA‑TTATTTTAAAATAATT‑AAATATAAATCAATTTTATTGTGGTTTTTTTTTTTTTTTTTACAAAAATATAat                       <  1:5259729/123‑1 (MQ=255)

                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCt                                                                         <  1:1686269/78‑1 (MQ=255)

                                                                                                          aGCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCt                                                                         >  2:1686269/1‑78 (MQ=255)

                                                                                                           taaaattttttAATAATTTTTAATTTTTATAAATTTATTTTTTTTAAAAATTTTTAA‑TTATTTTAAAAAAATTAATATTTAAATAAATTTTTTCCT‑GGTTTTTTTTTTTTTTTTACAAAAATATAatt                      <  1:4283297/123‑1 (MQ=255)

                                                                                                           gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAATTATAatt                      >  1:451722/1‑128 (MQ=255)

                                                                                                           gCACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATATAatt                      >  1:6429858/1‑128 (MQ=255)

                                                                                                            cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAAAAATAAAattt                     >  1:1000714/1‑128 (MQ=255)

                                                                                                            cACTTTTTTAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTAAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACTAAAATATAattt                     >  2:4795110/1‑128 (MQ=255)

                                                                                                               ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCG‑TTTTTTTTTTTTTTTTACTAAAATATTatttattt                 >  2:6824525/1‑128 (MQ=255)

                                                                                                               ttttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATAAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTAAAATAAAAAAattatat                  >  1:1103401/1‑124 (MQ=255)

                                                                                                                 ttttAAAAAATTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTC‑TGTTTTTTTTTTTTTTTACAAAAATATAATATATTTGa               >  2:4430008/1‑128 (MQ=255)

                                                                                                                 ttttAAAAAATTTTTAATTTTAAAAATTATATT‑TTTTTTAAATTTTTAAATTTATTTAAAAATCATT‑‑TTAATTAATAAATTTTTTTGTTGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGa               <  2:2363476/128‑1 (MQ=255)

                                                                                                                  ttaaaaaaaTTTTAATTTTAAA‑AACTTAAATATTTTTAAAATTTTTAAA‑TTATTTTAAAATCAAT‑ATCTATTAATCAATTTTTTGCTCG‑TTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt             <  1:6074729/125‑1 (MQ=255)

                                                                                                                   ttATAAATTTTAAATTTATAATTA‑TTAATTTTTTTTAAAATTTTTAAA‑TTTTTTTAAAATAATT‑ATAAATTAAACATTTTTTTTGTTCTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGAtt             <  2:3245358/128‑1 (MQ=255)

                                                                                                                         aaTTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTAAAAAAAATAAAATATATTtaaataaaat        >  1:347491/1‑126 (MQ=255)

||

AAATTAAATTCATTCATACTTTTAGTCTCATAAATCTAAGTTTCTAAAATTATTTTATCATTTAAAAAAATAATACTTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAATTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAA‑TTATTTTCAAATCATT‑ATCTATTAATCAATTTTATGCTCGTTTTTTTTTTTTTTTTTACAAAAATATAATTTATTTGATTAAACGTATATT  >  CP016816/420031‑420285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`