BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	420,234	1	.	T	44.2%	50.2 / inf	756	intergenic (‑517/+326)	JCVSYN2_01895/JCVSYN2_01900	30S ribosomal protein S10/tRNA‑Thr
Reads supporting (aligned to +/- strand): ref base . (253/168); new base T (245/89); total (498/258)
Fisher's exact test for biased strand distribution p-value = 1.48e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-05

Only 100 of 925 total aligned reads displayed.

TTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAATTAAATATATAACAATGTAAGTTTTAAA  >  CP016816/420107‑420361

 tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑t                                                                                                                                   >  2:897872/1‑128 (MQ=255)

 tttATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑t                                                                                                                                   >  1:2634027/1‑128 (MQ=255)

  ttattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑tt                                                                                                                                  >  1:3465537/1‑128 (MQ=255)

   tattaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑ttt                                                                                                                                 >  2:531260/1‑128 (MQ=255)

   tattaGGTGATATATTATAAAACTACTATCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑A‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑ttt                                                                                                                                 <  2:2573089/128‑1 (MQ=255)

    attaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑tttt                                                                                                                                >  1:436135/1‑128 (MQ=255)

    attaGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑A‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑tttt                                                                                                                                <  1:888367/128‑1 (MQ=255)

          tcatataatataAAAATAATATCACTTTTTTAAAAAA‑TTTTTAATTTTAAAAAATTAATTATTTTTAAAATTGTTAAATTATTTTCAAAACATTATATATTAAACAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑tttttttttt                                                                                                                          <  2:753659/126‑1 (MQ=255)

              atatTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑tttttttttttttt                                                                                                                      >  2:3200445/1‑128 (MQ=255)

               tatTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑ttttttttttttttt                                                                                                                     >  1:641245/1‑128 (MQ=255)

                  ttacaaaaTAATACCACTTTTTTAAAAAATTTTTTAATTTAAA‑AACTTTAATTTTTTTAAAAATTTTAAATTTATTTAAAATCATTATAAATTAATCAA‑‑A‑‑‑TTTA‑‑TGG‑‑‑C‑C‑G‑‑gttttttttttttttttt                                                                                                                  <  2:3374909/124‑2 (MQ=255)

                  tAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑tttttttttttttttttt                                                                                                                  >  2:3750446/1‑127 (MQ=255)

                     ataattatatttaCTTTTTTAAAAAA‑ATTTTAATTTTTATAAATTAATTATTTTTAAAATTTTTTAATTTTTTTAAAATAATTATTTATTAATAAA‑‑T‑‑‑TTTT‑‑TTT‑‑‑T‑T‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACaa                                                                                                            <  2:2802362/116‑1 (MQ=255)

                       catatctaTCAATTTTTTAAAAAA‑TTTTTAATTTTAAAAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATAATATATTAATAAA‑‑T‑‑‑TTTA‑‑TTC‑‑‑T‑C‑G‑‑tttttttttttttttttttacaa                                                                                                             <  1:1489284/123‑7 (MQ=255)

                       aactattttcaattttttAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTAAAATTGTTAAATTATTTTCAAATCATTATTTTTTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑CTT‑‑TTTTTTTTTTTTTTTTT‑‑‑ACaaa                                                                                                           <  2:3792431/116‑1 (MQ=255)

                       aaCTACTAGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACaaaa                                                                                                          >  1:105494/1‑128 (MQ=255)

                       aaCTACTAGCACTTTTTTAAAAAA‑TTTTTAATCTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGA‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTATT‑‑‑aaaaaa                                                                                                          >  1:1688717/1‑128 (MQ=255)

                       aaCTACTACCACTTTTTTAAAAAA‑TTTTTAATTTTAAAAAAAGAAATATTTTTAAAAATTTTAAAAATTTTTCAAATCAATATAAATAAAACAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑C‑C‑G‑‑ttttttttttttttttttttaca                                                                                                             <  1:3186524/128‑7 (MQ=255)

                        aataatatcACTTTTTTAAAAAA‑TTTTTTTTTTTAATAACTTAATAATTTTTCAAATTGTTAAATTTTTTTCAAATTATTATTTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑G‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACaaaaa                                                                                                         <  1:3826897/120‑1 (MQ=255)

                         atactaTTACTTTTTTAAAAAA‑TTTTTAATTTTAAAAACTTAATTATTTTTAAATTTTTTAAATTATTTTAAAATAATAATATATTAATAAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑A‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑ACaaaaa                                                                                                         <  1:3950446/127‑1 (MQ=255)

                          tatttgaagttttttAAAAAT‑TTTTATTTTTAATTAA‑TTTATTATTTTTAAATTTTTTTAATTTTTTTTAAATTTTTTTTTATTAATAAA‑‑T‑‑‑TTTT‑‑TTC‑‑‑T‑T‑GT‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAata                                                                                                       <  2:1798424/119‑1 (MQ=255)

                          tactaGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAata                                                                                                       >  1:2213307/1‑128 (MQ=255)

                          taatatattttttttAAAATA‑TTTTTTATTTTAATTAATTATTTTTTTTTTAATTTTTTTAATTTTTTTAAAATAATTATTTATTAATAAA‑‑T‑‑‑TTTA‑‑TTT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑Taaaaaaat                                                                                                       <  2:2329163/119‑3 (MQ=255)

                            ctaGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTT‑‑TTA‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑Aaaaaaaatat                                                                                                     >  1:1420828/1‑124 (MQ=255)

                             taGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAAtata                                                                                                     >  1:3148894/1‑128 (MQ=255)

                             taGCACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATaa                                                                                                    >  1:3245417/1‑128 (MQ=255)

                              atcaattttttAAAAAA‑TTTTTAATTTTAATAATTTATTTATTTTTAAAATTTTTAAATTTTTTTAAAATCATTATTTATTAATAAT‑‑T‑‑‑TTTT‑‑TTC‑‑‑T‑CTT‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATaa                                                                                                    <  2:2485835/123‑1 (MQ=255)

                                cACTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATAAAatt                                                                                                  >  2:1241832/1‑128 (MQ=255)

                                cACTTTTTTAAAAAA‑TTTATAATTTAAATAACTGAATTATATTTAAAATTTTTAAATTATTTTATAAAAATTATCTAATAAACAA‑‑T‑‑‑TTTA‑‑TTCACTT‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATaa                                                                                                    <  1:2548647/128‑1 (MQ=255)

                                aaattttttAAAAAA‑TTTTTAATTTTAAAAAATTAATTATTTTTAAAATTTTTAAATTTTTTTAAAATAATTATATATTAATAAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAat                                                                                                   <  2:3281300/125‑1 (MQ=255)

                                  cTTTTTTAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAattta                                                                                                >  1:2702939/1‑128 (MQ=255)

                                   ttttttAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑CTT‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATAtaattaat                                                                                               >  1:3976617/1‑128 (MQ=255)

                                   ttttttAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATAAAatttat                                                                                               >  1:850554/1‑128 (MQ=255)

                                   ttttttAAAAAA‑TTTTTAATTTTAATAAATGTATTTTTTTTATAATTTTTTAATTTTTTTAAAATAATTATATATTAATAAA‑‑T‑‑‑TTTT‑‑TTC‑‑‑T‑A‑GT‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAatttat                                                                                               <  1:3287115/128‑1 (MQ=255)

                                     ttttAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑AAAAAAAAATAatttatt                                                                                              >  1:2491716/1‑128 (MQ=255)

                                     ttttAAAAAA‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAatttattt                                                                                             >  1:3611593/1‑128 (MQ=255)

                                            aaa‑TTTTTAATTTTAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCTAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATTaaa                                                                                      >  1:120612/1‑128 (MQ=255)

                                                  tttaattttaatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TTC‑‑‑T‑C‑G‑‑‑TTTTTTTTTTTTTTTT‑‑‑AAAAAAAAATAATTTTATTTATTAAACTTaaat                                                                               >  1:3410964/10‑128 (MQ=255)

                                                  tttaattttaatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTGATTAAAAGta                                                                                  >  2:1104519/10‑128 (MQ=255)

                                                    taattttaatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATTAAACTTatat                                                                               >  1:821588/8‑128 (MQ=255)

                                                     aattttaatTACTTAATTTTTTTTAAAATTGTTAATTTATTTTTTAATAATTATTTATTAATAAA‑‑T‑‑‑TTTA‑‑TTA‑‑‑T‑C‑‑‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTa                                                                            <  2:3688273/122‑1 (MQ=255)

                                                       tttttatAAATTAATTTTTTTTTAATTTTTTTAATTTTTTTAAAATAATTATATATTAATTAAT‑T‑‑‑TTTT‑‑TTT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTGATTAAACGTATATTTa                                                                            <  1:573052/123‑1 (MQ=255)

                                                         ttaaaaaattaaTTTTTTTTTAAATTTTTTAAATTTTTTAAAATAATAATTAATTAATCAA‑‑TTTATTTT‑‑TTT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTa                                                                            <  2:3559322/118‑1 (MQ=255)

                                                          ttatAAATTAATTATTTTTAAAATTTTTAAATTATTTTAAAATAATTATATATTAATAAA‑‑T‑‑‑TTTT‑‑TTC‑‑‑T‑CTT‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGt                                                                         <  2:1538499/126‑1 (MQ=255)

                                                           aataaatttatttttTTTTAATATTTTAAATTTTTTTTAAATTATTATATATTTATTAA‑‑T‑‑‑TTTAT‑TTT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGtt                                                                        <  1:4018816/119‑1 (MQ=255)

                                                           aatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAGTCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTGATTAAAAGTATATTTATGt                                                                         >  1:3361907/1‑128 (MQ=255)

                                                           aatAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAAAATAATTTATTTGATTAAACTTATAttatttttt                                                                       >  1:1000582/1‑121 (MQ=255)

                                                            atAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTTATTAAAATTATATTTATGtt                                                                        >  1:496298/1‑128 (MQ=255)

                                                            atAAATTAATTTTTTTTTAAATTTTTTAATTTTTTTTAAATTATTATTTTTTATTAAATTT‑‑‑TTTT‑‑TTT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGtt                                                                        <  2:2273449/128‑1 (MQ=255)

                                                             tAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATAATTATATATTAATAAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑‑‑‑TTTTTTTTTTTTTTTTA‑‑‑AAAAAAAAAAATTTTATTTAATAAAAATTAT‑TTTATtttttaaa                                                                   >  1:2817739/1‑124 (MQ=255)

                                                                 ttaaTTATTTTTAAAATTGTTAAATTTTTTTTAAATTAATATATATTAATCAA‑‑T‑‑‑TTTA‑‑TTT‑‑‑T‑C‑G‑‑‑TTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATaaaa                                                                <  1:3871028/126‑1 (MQ=255)

                                                                 tGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑AAAAAAATAAAATTTATTTTATTAAAATTATATTTATTTTtaata                                                                   >  1:1276418/1‑128 (MQ=255)

                                                                  ttatttttTTTTAATTTTTTAAATTATTTTAAAATATTTTTTTTTTAATAAATTT‑‑‑TTTT‑‑TTT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTtaataa                                                                  <  2:361695/126‑1 (MQ=255)

                                                                  gatttattTTTCATATTTTTAAATTATTTTCAAATCATTATATATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATCTATGTTTAATaaaa                                                                <  2:3245417/125‑1 (MQ=255)

                                                                     ttattTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATAAAAATTATATTTATTTTTAATaaaaa                                                               >  1:2363287/1‑128 (MQ=255)

                                                                     ttattTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATATTTTATTTTATAAAACGTATTTTTTTTTTTAATAAAAAt                                                              >  2:3054525/1‑128 (MQ=255)

                                                                     ttattTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAAAATAATTTATTTTATTAAACGTATATTTATTTTTAAAAAAAAtt                                                             >  1:3582589/1‑127 (MQ=255)

                                                                      tttttttAAATTTTTTTAATTTTTTTATTATTTTTATTTATTAATTAAT‑T‑‑‑TTTT‑‑TTT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATg                                                             <  1:3748169/126‑1 (MQ=255)

                                                                      tattTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATTAAAATTATATTTTTGTTTTATaaaatttt                                                            >  1:1593057/1‑124 (MQ=255)

                                                                      tattTTTAAAATTTTTAAATTATTTTAAAATAATTTTTTATTAATCAA‑‑T‑‑‑TTTA‑‑TTC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATgg                                                            <  2:3423758/128‑1 (MQ=255)

                                                                        tttttCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATTAAACTTATATTTATTTTTAATAAAAAttta                                                           >  1:2416663/1‑125 (MQ=255)

                                                                         ttttCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑‑‑‑‑TTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTTTTTGATTAAACGTATATTTATGTTTAATAAAAATGGAaaagaa                                                     >  2:1051578/1‑128 (MQ=255)

                                                                           tttaattttttAAATTTTTTTATTATTATTTTTTATTAATAAA‑‑T‑‑‑TTTAT‑TTT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGaaaa                                                        <  1:989137/119‑1 (MQ=255)

                                                                            tgataTTTTTATATTATTTTTAATTAATTTTTTTTTAATCAA‑‑T‑‑‑TTTATTTGT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGaaaa                                                        <  1:3076354/124‑1 (MQ=255)

                                                                             cAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAT‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTA‑‑AAAAAAATAAAATTTATTTGTATAAACTTATATTTATTTTTAAAAAAAATCGaaaata                                                      >  2:3146182/1‑126 (MQ=255)

                                                                              aaaTTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATTAAAAGTATATTTATTTTTAATAAAAATTTAaaataa                                                     >  1:3710390/1‑128 (MQ=255)

                                                                                 ttGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTTTTTTATTAAACTTATATTTATTTTTAATAAAAATGTAAAATAAAGt                                                  >  1:2273439/1‑128 (MQ=255)

                                                                                 ttGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑‑‑TTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGaa                                               <  2:3311875/128‑1 (MQ=255)

                                                                                   gTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATTAAATTTATTTAATTAAACGTATATTTATTTTTATTAAAAATGAAaaataaattaa                                                >  2:1969886/1‑124 (MQ=255)

                                                                                     tAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATTTATTTTATTTGTTTAAACGTATATTTTTTTTTAAAAAAAATTTAAAAGAATGttatt                                              >  2:3869559/1‑124 (MQ=255)

                                                                                      tatttattTTTATATTATTATATATTTATATA‑‑T‑‑‑TTTAT‑TTT‑‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAAATTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAAtt                                             <  1:679296/125‑1 (MQ=255)

                                                                                      aaaTTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑AAAAAAATAAAATTTATTTTATTAAACGTTTTTTTATTTTTAATAAAAATGTAAAATAAATTGAAt                                              >  1:300219/1‑128 (MQ=255)

                                                                                       attttttttAAAATAATTATTTATTTATTAA‑‑T‑‑‑TTTT‑‑TTT‑‑‑TAT‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACTTATATTTATTTTTAATAAAAATGGAAAAGAAAGTGAATTg                                            <  1:1390900/123‑1 (MQ=255)

                                                                                        attattTTCAAATTATTATATATTAATAAA‑‑T‑‑‑TTTT‑‑TTCT‑‑T‑T‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGt                                           <  2:3517645/128‑1 (MQ=255)

                                                                                         ttattTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATTAAACGTATATTTATTTTTAATAAAAATTTAAAAAAAATTGAAtttta                                          >  1:350756/1‑125 (MQ=255)

                                                                                         ttattTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTA‑‑‑ACAAAAATATAATTTTTTTTATTAAACGTTTATTTATTTTTAATAAAAATTTAAAATAAATTTAAttttaa                                         >  1:928737/1‑124 (MQ=255)

                                                                                            ttttCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTTA‑AAAAAAATATAATTTATTTTTTTAAACTTATATTTATTTTTAATAAAAATGTaaaaaaaatataattttaaa                                        >  1:1983928/1‑116 (MQ=255)

                                                                                             tttCAATTCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑AAAAAAATAAAATTTTTTTTATTAAAAGTATATTTATTTTTAATAAAAATTGATAATAAATTTAAtttaaata                                       >  1:2116097/1‑122 (MQ=255)

                                                                                             tttCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAAATAAATTTTTTTGATAAAAATTATATTTATTTTTAATAAAAATGTAAAATAAATTATATTTACataat                                     >  2:2131350/1‑128 (MQ=255)

                                                                                             tttCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑‑‑TTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTATTGTTTAATAAAAATGGAAAAGAAAGTGAATTTTCAtaataa                                   >  2:3111375/1‑128 (MQ=255)

                                                                                               ttaaaTTATTATATATTTATAAA‑‑T‑‑‑TTT‑‑‑TTT‑‑‑T‑T‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAATTATAATTTATTTGATTAAAAGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGTCATaataat                                  <  2:2638443/126‑1 (MQ=255)

                                                                                                  aaTCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTTTTAAACGTTTATTTATTTTTAATAAAAATGTAAAAAAAATTGAATTTTAATAAtaatt                                 >  2:416562/1‑128 (MQ=255)

                                                                                                  aaTCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAAAATAATTTATTTTATTTAAAGTATATTTTTTTTTAAAAAAAATTGAAAAAAAATTTAATTTTAATAAaaattt                                >  1:3332267/1‑128 (MQ=255)

                                                                                                  aaTCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTA‑‑‑AAAAAAATATAATTTATTTTATAAAAAATATATTTTTTTTAAAAAAAAATTAAAAAAAAAATTAATTTTATTAAaaatta                                >  1:2076325/1‑127 (MQ=255)

                                                                                                     cATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GTTTTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTTTTTTTTTAAACGTATTTTTATTTTTATAAAAAAAGTAAAATAAAGTAAATTGGTAT‑Ataatttta                              >  2:2728353/1‑126 (MQ=255)

                                                                                                        tatttattAATTAA‑‑A‑‑‑TTTT‑‑TTC‑‑‑T‑C‑T‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGTCATAATAATttaattaa                          <  2:2991061/128‑1 (MQ=255)

                                                                                                          tCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTTATTAAACGTATATTTATTTTTAATAAAAATGAAAAATAAAGTGAATTTTCATAATAATTTAATTaaa                         >  2:1399945/1‑128 (MQ=255)

                                                                                                          tCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATAAAATTTTTTTTATTAAACTTATATTTATTTTTATAAAAATTGAAAAATAAATTTATTTTTAATATTAATTTAATTaaa                         >  2:3470448/1‑128 (MQ=255)

                                                                                                           cTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATTAAAATTATTTTTATGTTTAATAAAAATGTAAAATAATTTGAATTGTAATAATAATTTATTTAAat                        >  1:2950946/1‑128 (MQ=255)

                                                                                                           cTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑‑TTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTGATTAAACGTATATTTATTTTTAATAAAAATGTAAAAGAAATTTAATTGTAATAAAAATTTAATTAAatat                      >  1:1940222/1‑128 (MQ=255)

                                                                                                            tATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTTTAAAAAAAATAAAATTTATTTAATTAAAATAAATTTTATTTTTAATAAAAATGTAAAAAAAATTAAATTTTAAAAATAATTTAATTaaa                         >  1:2329359/1‑128 (MQ=255)

                                                                                                            tATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATTAAAAGTATATTTATTTTTAATAAAAATGGAAAAGAAAGTTATTTGTCATAATAATTTATTTAAatat                      >  1:3674109/1‑128 (MQ=255)

                                                                                                             aTTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTTATTAAACGTATATTTATTTTTAATAAAAATGTAAAAGAAATTGAATTTTCATAATAATTTAATTAAatata                     >  1:2751184/1‑128 (MQ=255)

                                                                                                              ttAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑‑‑TTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTTATTAAACTTATATTTTTTTTTAATAAAAATGTAAAATAAAGTGAATTGTAATAATAATTTAATTAAATATTTaa                  >  1:1142848/1‑128 (MQ=255)

                                                                                                                aaTCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑‑TTTTTTTTTTTTTTTT‑‑‑ACAAAAATAAAATTTATTTGATAAAACTTATTTTTATGTTTAATAAAAATGGAAAATAAAGTTATTTTTACTAATAATTAAATTAATAATATAAc                 >  2:3588755/1‑128 (MQ=255)

                                                                                                                  tCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTGATTAAACGTATATTTTTTTTTAATAAAAATGTAAAATAAAGTTAATTTTAATAATAATTTAATTAAATATATAAc                 >  1:724341/1‑128 (MQ=255)

                                                                                                                   gaa‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑T‑GT‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTGAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAATTAAATATATAACa                <  2:3981499/127‑1 (MQ=255)

                                                                                                                   cAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTTTA‑AAAAAAAAAAAATATAATTTATTAAAATAATTTTTTTTTTTATTAAAAATGTAAAAAAAATTAAAATTTAATAATAATTAAAataaataaataaa                 >  1:1807414/1‑127 (MQ=255)

                                                                                                                            tttA‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATTTTTATTTTTAATAAAAATGTAAAAGAAATTTAATTTTCATAAAAATTTAATTAAATATATAAAAAttt            >  1:766350/1‑126 (MQ=255)

                                                                                                                               a‑‑TGC‑‑‑T‑C‑GT‑TTTTTTTTTTTTTTTTT‑‑‑AAAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATTGAAAAAAAAATAAATATTAATAATAATTAAATTAAAAAAATAACAATTTAAg         >  1:1343993/1‑128 (MQ=255)

TTTTATTAGTTGATATATTAGAAAACTACTAGCACTTTTTTAAAAAA‑TTTAAAATTAAAATAACTGAATTATTTTTCAAATTGTTAAATTATTTTCAAATCATTATCTATTAATCAA‑‑T‑‑‑TTTA‑‑TGC‑‑‑T‑C‑G‑‑TTTTTTTTTTTTTTTTT‑‑‑ACAAAAATATAATTTATTTGATTAAACGTATATTTATGTTTAATAAAAATGGAAAAGAAAGTGAATTGTCATAATAATTTAATTAAATATATAACAATGTAAGTTTTAAA  >  CP016816/420107‑420361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`