BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP016816	165,222	G→A	intergenic (+186/‑356)	JCVSYN2_00665 → / → JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP016816	165,222	0	G	A	99.0%	4189.5 / 21.6	1358	intergenic (+186/‑356)	JCVSYN2_00665/JCVSYN2_00670	tRNA N6‑adenosine(37)‑N6‑threonylcarbamoyltransferase complex dimerization subunit TsaB/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (805/530); minor base . (2/11); total (807/551)
Fisher's exact test for biased strand distribution p-value = 1.30e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1391 total aligned reads displayed.

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATA  >  CP016816/165097‑165350

cTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTaaaaaa                                                                                                                                  >  1:1053074/1‑128 (MQ=255)

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAaat                                                                                                                                 >  2:3261201/1‑128 (MQ=255)

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                                 >  1:1921294/1‑128 (MQ=255)

 tttAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaat                                                                                                                                 >  1:1670973/1‑128 (MQ=255)

   tAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaatta                                                                                                                               <  1:1008035/128‑1 (MQ=255)

    aaTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaa                                                                                                                              <  1:456739/128‑1 (MQ=255)

     aTTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAaattaat                                                                                                                             >  2:4028797/1‑128 (MQ=255)

        gTATTACTTTACTAATTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTg                                                                                                                          <  1:857959/128‑1 (MQ=255)

          aTTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGaa                                                                                                                        >  2:3603505/1‑128 (MQ=255)

           ttactttactAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAAc                                                                                                                       >  1:3904527/1‑128 (MQ=255)

                  actAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                                <  1:2454489/128‑1 (MQ=255)

                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                               <  1:2313503/128‑1 (MQ=255)

                   ctAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaa                                                                                                               >  2:3139951/1‑128 (MQ=255)

                    tAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaaaa                                                                                                              <  2:2869325/128‑1 (MQ=255)

                       ttGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATa                                                                                                           >  1:1288712/1‑128 (MQ=255)

                        tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATTCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAg                                                                                                          <  1:404030/128‑1 (MQ=255)

                        tGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATat                                                                                                          >  1:2780/1‑127 (MQ=255)

                           taataTTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtt                                                                                                       >  2:461506/1‑128 (MQ=255)

                                     tttCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCtttttattttta                                                                                             >  1:153357/1‑127 (MQ=255)

                                         aTTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGAttattttttttttttt                                                                                         >  1:2640687/1‑128 (MQ=255)

                                              tttATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaaa                                                                                                                >  1:988583/1‑100 (MQ=255)

                                                 ttagCACTTTTATTAATTTTTAATTAAATAAAAATATTATTTTTTTTGTTATTTTTTTTATTTTCAATTTTTTAAAAAAATTATTTTAAAATGTTAAAAAAATC‑TTTTTTTTTTTTTTTTTTATAATg                                                                                <  2:1420986/125‑1 (MQ=255)

                                                    gcaattgtttttattattAATAAAATAAAAATATTATTTTTTTTTTTATTTTTTGTTTTTTTAAATTTTTTAAAAATTTATTTTTAATTTTAAAAAAT‑‑TTCTTTTTTTTTTTTTTTTTATAATGGaaa                                                                            <  2:2383633/116‑1 (MQ=255)

                                                     cACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCttttttttttttttttttaaatgtaaat                                                                             >  2:1765067/1‑118 (MQ=255)

                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAataaa                                                                        >  1:3140645/1‑126 (MQ=255)

                                                        ttGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAAtata                                                                        >  2:3519293/1‑128 (MQ=255)

                                                          gTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTaa                                                                                                                 >  1:2129081/1‑87 (MQ=255)

                                                           tATTAATTATTAAGAAACATAAACTATTATATTTTTTTATATTTTTTTTATATTCAAATTTTTAAAAAAATAATTTGAAATTTTAAAAAAAA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                    <  2:3471272/128‑1 (MQ=255)

                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAATTTTTAAAA‑AATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTATTTATAATTTAAATATAAAtt                                                                    >  1:2845053/1‑128 (MQ=255)

                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTATTAATTTAAATATaa                                                                       >  2:872348/1‑128 (MQ=255)

                                                           tATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTAAAATATAAAt                                                                     >  1:3400274/1‑128 (MQ=255)

                                                            ttGAATTAGTAATTAACATAAACTTTTTTCTTTTTTTATTATTTTTTTATTTTCAAATTTTTTAAAAATTTATTTTTACTTGTAAAAAATTCC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAAtt                                                                    <  1:3826669/127‑1 (MQ=255)

                                                            aTGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTATA‑AAT‑AAAATATAAAtt                                                                    >  2:3877252/1‑128 (MQ=255)

                                                              gAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTAAAATGTAAATATAAAttt                                                                   >  1:2694665/1‑128 (MQ=255)

                                                               tttttttaataaAATAAATATATTTTATTTTTATTTATTTTTTTTATTTTAAAATTTTTATAAAATAAATTTGTAATTTAATAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                                <  2:1990291/122‑1 (MQ=255)

                                                                atttttattaaaatataaataTTTTTTTTTTATATATTTTTTTTTTATTCAAATTTTTAAAAATTTAATTTTAAATTTTAAAAAAT‑‑TTATTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                                <  2:602515/119‑1 (MQ=255)

                                                                attattAAAAAAATAAAAATATTTATTTTTTTTTTTTTTTTTTTTTTTTAAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAAATGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAg                                                                <  1:927911/128‑1 (MQ=255)

                                                                  tattaataaAATAAAAATTTTTTATTTTTATATATTTTTTTTATTTTCAAATTTTTAAAAAATTAATTTTAAATTGTAAAAAATTGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGt                                                              <  1:5691/125‑1 (MQ=255)

                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGTAAATAAAAAATTTatt                                                               >  2:1358406/1‑126 (MQ=255)

                                                                  tAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATTTAAATTTTattt                                                              >  2:2062262/1‑125 (MQ=255)

                                                                   attaaacaacaaaaacttTTATATTTTTATATATTTTTTGTTTTTTCAAAATTTTTAAAAATTAATTTTAAATTTTAAAAAAAAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑t                                                           <  1:3203880/121‑1 (MQ=255)

                                                                   aGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATTATTGAAATATAAATTTTAGGTa                                                             >  2:3066997/1‑128 (MQ=255)

                                                                      aaGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTattta‑ttt                                                         >  1:2197097/1‑128 (MQ=255)

                                                                      aaGAAACTAAAACTATTATATTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTCTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATTAATTTtattta‑ttat                                                        >  2:1904180/1‑126 (MQ=255)

                                                                        gAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTATTTATAATGGAAATATAAATTTTAGGTA‑TTttg                                                       >  2:3203490/1‑128 (MQ=255)

                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATGTA‑tttttt                                                      >  1:1441409/1‑126 (MQ=255)

                                                                         aaactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATATTTTAAATATAAATTTTatttttattttt                                                      >  2:1155301/1‑117 (MQ=255)

                                                                          aactaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTATTTTTTTTTTTAAAATTTAAATATAAATTTTAGttt‑tttttt‑tt                                                   >  2:2123505/1‑127 (MQ=255)

                                                                           actaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATTTA‑Tttttt‑tt                                                   >  1:79702/1‑127 (MQ=255)

                                                                           acaaaaaataTTATATTTTTTTTTATTTTTTGTATATGCAAATTTTTAAAAAATTTATTTGAACTTTTAAAAAATA‑‑‑TGTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTtgt‑tgt                                                  <  1:3553521/125‑1 (MQ=255)

                                                                            ctaaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTGTAAATAAAAATTTTAGTTA‑Tttttt‑ttt                                                  >  1:735834/1‑126 (MQ=255)

                                                                            ataaaaattTTATATTTTTTTATTTTTTTTTTATATTAAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTttgt‑t                                                    <  2:1854155/127‑1 (MQ=255)

                                                                            ataaaaataTTATATTTTTTTATATTTTTTTTATATTCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTtgt‑tgt                                                  <  1:1144723/127‑1 (MQ=255)

                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTA‑Tttttt‑tt                                                   >  1:1247561/1‑127 (MQ=255)

                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTATTTA‑Tttttt‑ttt                                                  >  1:3491284/1‑126 (MQ=255)

                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTTAAATATAAATTTTATGTA‑Attttt‑ttta                                                 >  2:2717354/1‑125 (MQ=255)

                                                                             taaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCt                                                >  2:230711/1‑128 (MQ=255)

                                                                             ataaaataTTTTATTTTTTTAAATTTTTTTTTTATTCAAATTTTTTAAAAAATAAATTTAACTTTTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTtgt‑tgt                                                  <  1:2085335/126‑1 (MQ=255)

                                                                              aaaactaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATGGAAATTTAAATTTTAGTTA‑TTTttt‑tgtat                                                >  2:929701/1‑126 (MQ=255)

                                                                               aaaattatatttttttttattttttttTTATTTTCAAATTTTTAAAAAATTAATTTTAAATTTTAAAAAATA‑‑‑TTTTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTaa                                              <  2:149076/121‑1 (MQ=255)

                                                                                aaattttatATTTTTTCATATTTTTTTTATATGCAAATTTTTAAAAAATTAATTTTTAATTTTAAAAAATATC‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTa                                               <  1:2558583/123‑1 (MQ=255)

                                                                                 actaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGAAAAAATAAATTTTATTTA‑Tttttt‑tttaaaat                                             >  2:1067817/1‑121 (MQ=255)

                                                                                  ctaTTATCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGT‑AAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAATTTAAATATAAATTTAAGTTA‑TTTTTT‑TTTATAATTa                                           >  2:691521/1‑128 (MQ=255)

                                                                                   tattttCGTTTTTTATTTTTTTTGTTTATGCAAATTTTTAAAAAATTAATTTTAACTTTTAAAAAAT‑‑TTCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTa                                           <  1:3620893/128‑1 (MQ=255)

                                                                                   tattatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGATA‑TTTTTT‑TTTCTAATTaa                                          >  1:2004179/1‑127 (MQ=255)

                                                                                    attttattttttTATTTTTTTTGTATATTAAAATTTTTTAAAAATTAATTTGAAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTAt                                          <  2:2601677/122‑1 (MQ=255)

                                                                                    attatCTTTTTAGATATTTTTTGTATTATAAAATTTTTAAAAAATTAATTTGAACTTTTAAAAATTA‑‑TGTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTAt                                          <  1:1763139/128‑1 (MQ=255)

                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTTAAAATATAAATTTTATTTA‑Tttttt‑tttaaatttata                                         >  1:1394013/1‑117 (MQ=255)

                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTA‑Tttttt‑ttttaaattatc                                         >  2:2500349/1‑117 (MQ=255)

                                                                                     ttatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAAATATAAATTTTAGTTA‑TTTTGT‑TGTATATTTATCt                                        >  2:3935287/1‑128 (MQ=255)

                                                                                      tttttttttATTTATTTTTTTTATATTTAAATTTTTTAAAAATTTATTTTTAATTTTAAAAAATA‑‑TTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTATCt                                        <  1:1589530/124‑1 (MQ=255)

                                                                                      tataTTTTTATATATTTTTTTTTTATTATAATTTTTAAAAAATTATTTTTAATTTGTAAAAAATTG‑‑TTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTATCt                                        <  2:1236225/128‑1 (MQ=255)

                                                                                      tatCTTTTTAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTA‑TTTTTT‑TTTATAAgataata                                       >  1:3974002/1‑122 (MQ=255)

                                                                                       atCTTTTTAGATATTTTTTGTATATGCAAATTTTTTAAAAATTAATTTGCTCTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATTTAAATTTTA‑GTA‑TTTTTTGTGTATAAGTTTCTa                                       >  2:2784462/1‑128 (MQ=255)

                                                                                           ttttATTTATTTTTTTTTTATTTAATTTTTTAAAAAATTAATTTTTAATTTTAAAAAATA‑‑‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTATCTaaaaaaa                                 <  2:158333/128‑1 (MQ=255)

                                                                                           ttttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑TTTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTAGTTT‑TTTTTT‑TGACTAATTAAATaaaa                                    >  2:2964147/1‑128 (MQ=255)

                                                                                            tttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTAT‑‑TTTAAATATAATTTTTAGTTT‑TTTTTT‑TTTGTATTTTTATaaaaaa                                  >  1:3344886/1‑128 (MQ=255)

                                                                                             ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATTATGTAAATTTAATTTTTAGTTA‑TTTTTT‑TTTATATTTATATaaaaaaa                                 >  2:1294475/1‑128 (MQ=255)

                                                                                             ttAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAAT‑TAAAATTTATTTATTTTTGT‑TTACTAATTATTTaaataaat                                >  1:3451451/1‑128 (MQ=255)

                                                                                              tAGATATTTTTTGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATTTAAATAAAAATTTAATATA‑TTTTTT‑TTTAAAATTATAaaaaaaaa                                 >  1:2900401/1‑128 (MQ=255)

                                                                                               atttatTTTTTTTTTATTTTAATTTTTTAAAAATTAAATTTAAATTTTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTATCTAAAAAAATa                               <  1:3243565/125‑1 (MQ=255)

                                                                                                tttatTTTTTTTTTTTTGAAATTTTTTAAAAATTAATTTTTAATTGTAAAAAATA‑‑‑TCTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTATCTAAAAAAATAGa                             <  1:1835104/126‑1 (MQ=255)

                                                                                                gATATTTTTTTTATATTTAAATTTTTAAAAAATTTATTTGAAATTGTAAAAAAAAG‑‑‑TTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTATCTAAAAAAATAGa                             <  1:517393/128‑1 (MQ=255)

                                                                                                     tttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTT‑TTTTTT‑TGTCTAATTATATAAAAAAATAGAAAgt                         >  2:3777067/1‑127 (MQ=255)

                                                                                                      ttttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGTAAATATAAATTTTAGTTA‑TTTTGT‑TGTATAATTATCTAAAAAAATATAAAGTaa                       >  2:2222182/1‑128 (MQ=255)

                                                                                                       tttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATTTAATTATAAATTTTATGTA‑TTTTGT‑TTTATAATTATATAAAAAAATATaaatt                         >  1:669442/1‑126 (MQ=255)

                                                                                                        ttGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTT‑TAATTTAAATATAAATTTTATTTA‑TTTTTT‑TTTATAATTTAAAAAAAAAATAaaaataaa                       >  1:2389828/1‑124 (MQ=255)

                                                                                                         tGTATATGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTA‑TTTTTT‑TGTATAAGTTTATAAAAAAATATAAAGTaaaa                     >  1:3089085/1‑128 (MQ=255)

                                                                                                           tataTGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTA‑TTTTGT‑TGTCTAAGATTCTAAAAAAATACAAATGAAAAAat                  >  1:618125/1‑128 (MQ=255)

                                                                                                            atatGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGTAAATAAAAATTTTATGTA‑TTTTTT‑TTTATAAGTATATAAAAAAATAAAAAGTAAAAAat                  >  2:3343672/1‑128 (MQ=255)

                                                                                                             tatGCAAATTTTTAAAAAATTAATTTTAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTAAAAAGTAAATATAAATTTTAGTTA‑TTTTTT‑TTTCTAATTATATAAAAAAATAAAAAGTAAAAAata                 >  1:3767730/1‑128 (MQ=255)

                                                                                                              atGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAAAATAAATTTTATGTT‑TTTTGT‑TGTAAAATTATATAAAAAAAAAAAATGAAAAAAatat                >  2:787633/1‑128 (MQ=255)

                                                                                                               tttaaaTTTTTTAAAAATTTATTTGAATATTTATAAAATA‑‑TGTTTTTTTTTTTTTTTTTATAATTGAAATATAAATTTTAGGTA‑TTTTGT‑TGTATAAGTATCTAAAAAAATAGAAAGGAAAAAATAtt               <  2:54950/125‑1 (MQ=255)

                                                                                                               tGCAAATTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGTTA‑TTTTTT‑TGTATAATTATATAAAAAAAATAAAAGTAAAAAatttat              >  2:3698915/1‑124 (MQ=255)

                                                                                                                  aaaTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATATTTTAATAATAAATTTTAGGTA‑TTTTTT‑TGTAAAATTATATAAAAAAATATAAAGTAAAAAATtttttt            >  1:3679781/1‑128 (MQ=255)

                                                                                                                   atttttTAAAAAATTATTTTTTA‑TTTTAAAAAATA‑‑TCTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTaa          <  2:3410069/126‑1 (MQ=255)

                                                                                                                    aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTT‑‑TAATGTAAATAAAAATTTATGGTA‑TTTTTT‑TTAATAATTAAATAAAAAAATAGAAAGTAAAAAATATTTTTaa          >  1:2258359/1‑128 (MQ=255)

                                                                                                                    aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTTTTTTTTTTTTTATAATGGAAATAAAAATTTTAGGTA‑TTTTTT‑TGTAAAAGTATATAAAAAAATAGAAAGTAAAAAATATTTTTaa          >  1:3004824/1‑128 (MQ=255)

                                                                                                                    aTTTTTAAAAAATTAATTTGAACTTGTAAAAAATAGC‑‑TTTTTATTTTTTTTTTTA‑AAAGGAAAAAAAAAATTTTGGTA‑TTTTTT‑TTTTTAATTATAAAAAATAAAAAAAAGTAAAAAAAATTTTTtaa         >  1:1330820/1‑128 (MQ=255)

                                                                                                                         taaaaaaTTAATTTGAACTTGTAAAAAATAGC‑‑‑TTTTTTTTTTTTTTTTAAAATGTAATTATAAATTTTAGGTA‑TTTTTT‑TGTATAATATTATAAAAAAATAAAAAGAAAAAAATATTTTAAAAATTaa    >  2:1630764/2‑128 (MQ=255)

CTTTAATTGTATTACTTTACTAGTTGATAATATTTAGTTTCATTAATTTATCGCACTTGTATGAATTAGTAAGAAACTAAAACTATTATCTTTTTAGATATTTTTTGTATATGCAAATTTTAAAAGAATTAATTTGAACTTGTAAAAAATAGCTTTTTTTTTTTTTTTTTTTATAATGGAAATATAAATTTTAGGTA‑TTTTGT‑TGTCTAAGTATCTAAAAAAATAGAAAGGAAAAAATATTGTTAAAAGTAATA  >  CP016816/165097‑165350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`