Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,378,8020AC53.3% 30.6 / 41.8 30V48G (GTA→GGA) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
Reads supporting (aligned to +/- strand):  ref base A (11/3);  new base C (0/16);  total (11/19)
Fisher's exact test for biased strand distribution p-value = 6.66e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-05

CCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAA  >  NC_000913/4378655‑4378941
                                                                                                                                                      |                                                                                                                                           
ccTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑accg                                                                                                                                          <  1:491933/151‑1 (MQ=255)
   cGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑accgcca                                                                                                                                       <  1:67969/151‑1 (MQ=255)
    gAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑accgccac                                                                                                                                      >  1:927445/1‑151 (MQ=255)
     aGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑accgccacc                                                                                                                                     <  2:686486/151‑1 (MQ=255)
             cGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATAcc                                                                                                                             >  1:580343/1‑151 (MQ=255)
                                  tAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                                                        >  1:349255/1‑151 (MQ=255)
                                   aaaTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                                                        >  1:282063/1‑150 (MQ=255)
                                   aaaTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                                                        >  1:387246/1‑150 (MQ=255)
                                   aaaTGTTTGGCTGACCGACCCTAACTAAAACACCGTTTGCTGTGTGCGGGGCTGTTTTTGGGCGATGCCCGCAAAGCCCAAGG‑AAAATAAAAAGGTTTTCCCTTGAAAAA‑TT‑CCCGCCACCGCCATACCCGGGATCTTttgtt                                                                                                               <  2:435771/143‑1 (MQ=255)
                                        ttttGCTGACCGAACCAAACAAAAACACCTTTTGCGGTGGGAGGGGTTTTTTTTGGGCGATCTCCGCATCCCCCAAGGAAAAGTAAAAAGTTTTCCCT‑TAAAAAA‑TT‑CCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAg                                                                                                     <  1:886072/148‑1 (MQ=255)
                                          ttGCTGCCCGACCCTACCTCAAACACCGTTTGCTGTGTGAGGGGCTGTTTTTTGGCGATGTCCGCATACCCCCAGGAAAAGAAATAAGTTTTCCCC‑TAAAAAT‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCggg                                                                                                 <  2:124948/150‑1 (MQ=255)
                                              tGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAAt                                                                                            >  1:895797/1‑151 (MQ=255)
                                                 ccaacccTAACTAAAACACCGTTTGCTGTGTGACGGGCTGTTTTTGGGCGATGTCCGCATACCCCCAGGAAAAGTAAAAAGTTTTCCCT‑TAATAAT‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATtg                                                                                          <  2:812310/145‑1 (MQ=255)
                                                    acccTAACTAAAACACCGTTTGCTGTGTGAGGGGCTGTTTTTTGGCGATGTCCGCATACCCCCAGTAAAAGAAATAAGGTTTCCCC‑TGATAAT‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCg                                                                                      <  2:681990/149‑1 (MQ=255)
                                                    aaCTTAACTCAAACAACGTTTGCGGTGTGACGGGCTGTTTTTTGGCAATGTCCGCATAGCCCCA‑GAAAAGTTAAAAGGTTTTCCC‑TTAAAAA‑TTCCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGc                                                                                       <  1:59602/150‑1 (MQ=255)
                                                      ccTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGAt                                                                                    >  1:409463/1‑151 (MQ=255)
                                                                  aacGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGt                                                                        >  1:168108/1‑151 (MQ=255)
                                                                       ttGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAtt                                                                   >  1:467256/1‑151 (MQ=255)
                                                                         gCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttg                                                                 >  1:439678/1‑151 (MQ=255)
                                                                            gtgtgACGGGTTTTTTTTTGGCGATGTCCGAATGCCCCCAGGAAAAGTAATAGTTTTTCCCC‑TAATAAT‑TT‑CCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttgtt                                                               <  1:310176/150‑1 (MQ=255)
                                                                                gaggggCTTTTTTTGGGCGATGTCCGCATAGCCCCAGGAAAAGTAATAGGTTTCCCCC‑TGATAAA‑TT‑ACCGCCGCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAt                                                                    <  2:712616/138‑1 (MQ=255)
                                                                                   gggttttttttgGGCGATGCCCGCATACCCCCGGATAAAAAAATAAGTTTTCCCT‑TAATAAT‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAggg                                                          <  2:239787/141‑1 (MQ=255)
                                                                                               ggCTATGTCCGCAGACCCCCAGGAAAAGAAATAAGTTTCCCCT‑TAAAAAA‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAg                                           <  1:897724/151‑1 (MQ=255)
                                                                                                 cGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGc                                          >  2:946400/1‑150 (MQ=255)
                                                                                                               ccccAGGAAAAGAAAAAGGGTTTCCCC‑TAATAAT‑TC‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCg                                  <  2:58585/144‑1 (MQ=255)
                                                                                                                ccaaGGAAAAGTAAT‑AGGTTTTCCC‑TTAAAAATTT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGtaattaa                          <  2:413085/148‑1 (MQ=255)
                                                                                                                       aacataataaGTTTTCCTC‑TCTTAAA‑TC‑ACAGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAgg                     <  2:132724/145‑1 (MQ=255)
                                                                                                                       aaaGTAAAAAGGTTTCCCC‑TGAAAAA‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAgg                     <  2:220733/149‑1 (MQ=255)
                                                                                                                               aaGTTTTCCCC‑TAAAAAT‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGc             <  2:787799/149‑1 (MQ=255)
                                                                                                                               aaGTTTTCCCC‑TAAAAAT‑TT‑CCCCCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGcg            <  1:626949/150‑1 (MQ=255)
                                                                                                                                 gttttCCCC‑TAATAAA‑TT‑ACCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGcgtc          <  2:942455/148‑1 (MQ=255)
                                                                                                                                  gTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGcgtcgt        >  1:613969/1‑151 (MQ=255)
                                                                                                                                   tttCCCC‑TAAAAAA‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGc             <  1:631459/145‑1 (MQ=255)
                                                                                                                                         caagATAAT‑TT‑CCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGaa  <  1:934826/148‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                           
CCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCC‑TGATAAA‑TT‑ACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAA  >  NC_000913/4378655‑4378941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑