Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	436,516	0	C	A	51.8%	39.0 / 75.4	55	Q295K (CAA→AAA)	thiL	thiamine monophosphate kinase
Reads supporting (aligned to +/- strand):  ref base C (20/6);  new base A (0/28);  total (20/35)
Fisher's exact test for biased strand distribution p-value = 7.16e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-06

CTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTG  >  NC_000913/436366‑436661                                                                                                                                                       |                                                                                                                                                   cTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGc                                                                                                                                                    >  1:20900/1‑151 (MQ=255) cTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGc                                                                                                                                                    >  2:146317/1‑151 (MQ=255)    tctcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCaa                                                                                                                                                  >  2:540180/1‑150 (MQ=255)                    cGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCg                                                                                                                                 >  2:664088/1‑150 (MQ=255)                     ggAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGa                                                                                                                                >  2:359926/1‑150 (MQ=255)                       aaCGGGCGCTGCGCGGGGCCCTCTCGGGGGGTGAAGATTACGGGTTGTGTTTCACTGTGCGGGAACTGACCCGGGGCGCGCTGGATGTGGCTCTCGGACCCCGGGGCGTCCCTTTTCCCTGTATGGGGAAAATG‑ACCGCCGATATCGAAg                                                                                                                              <  2:702650/150‑1 (MQ=255)                         cAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGc                                                                                                                           >  1:77416/1‑151 (MQ=255)                          aGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCt                                                                                                                          >  2:108655/1‑151 (MQ=255)                                 gcgctgGGCGCTTTCTGGGGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGACCCGTGGCGCGCTGGATGTGGCTCCCGGACCCCGGGGCGTACCGTTTCCCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGc                                                                                                                           <  1:579814/143‑1 (MQ=255)                                      gggCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCg                                                                                                              >  2:248116/1‑151 (MQ=255)                                        gcgcTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTg                                                                                                            >  1:121325/1‑151 (MQ=255)                                          gcTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGAc                                                                                                          >  2:331686/1‑151 (MQ=255)                                           ctctctGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACCCCGGGGCGTACCGTTTACCTGTATGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGa                                                                                                           <  1:545580/149‑1 (MQ=255)                                            tctctGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACgg                                                                                                        >  1:545435/1‑151 (MQ=255)                                                 gggggTGAAGTTTACGAGTTGTGTTTCGCTGTGCCGAAACTGACCCGTGGCGCGGTGGATGGGGCTCTCGGCCCCCTGGGGGTCCTTTTTGCTTTTATCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAAc                                                                                                   <  2:145096/148‑1 (MQ=255)                                                 gggggTGAAGATTAGGAGTTGTTTTTCACTGTGCCGGACCTGAACCGGGGGGCGCTGGATGTGGCTCTCGGACCCTGGGGCGTCCCGTTTCCCTGTATCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCg                                                                                                      <  1:359972/145‑1 (MQ=255)                                                 ggCGGTGAAGATTACGAGTTGTGTTTCCCTGTGCCGGAACTGAACCGTGGCGCGCGGGATGTGGCTCTCGGACACCTGGGCGTCCCTTTTCCCTGTATGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGa                                                                                                     <  1:499869/149‑1 (MQ=255)                                                   cGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAAcc                                                                                                  >  2:466550/1‑150 (MQ=255)                                                     gTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGt                                                                                               >  2:414207/1‑151 (MQ=255)                                                        aaGATTAGGATTTGTGTTTCACTGTGCCGGAACTAAACCGTGGCGCGTGGGATGTGGCTCTCGGACCCCGGGGCGTCCCTTTTCCCTGTATGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGt                                                                                               <  1:56302/148‑1 (MQ=255)                                                         aGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTAGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTAc                                                                                            >  1:627858/1‑150 (MQ=255)                                                            ttgcgttttGTGTTTCACTGTGCCGGACCTGACCCGTGGCGCGTTGGGTTTGCCTCTCGGACACCGGGGCTTCCCTTTTCCCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACAt                                                                                          <  1:716703/142‑1 (MQ=255)                                                             tACGAGTTGTGTTTCACTGTGCCGAAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACGTGGGCGTACCGTTTCCCTGTATCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTa                                                                                        <  1:473517/150‑1 (MQ=255)                                                                 aGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTg                                                                                   >  1:619721/1‑151 (MQ=255)                                                                  gttttttttCCCTGTGCCGCAATTGCGCGGGGGCGCGGTGGATGGGGCTCTCGGACACCGGGGGGTCCTTTTTCCTTGAGTGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGAc                                                                                     <  2:759234/142‑1 (MQ=255)                                                                   ttGTGTTTCTCTGTGCCGGATCTGACCCGAGGCGCGTTGGATGTCGCTGCCGAACCCAGGGGCCCTCCTTTTACTTTTATCGAGAAAATG‑CCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTgg                                                                                  <  1:258015/150‑1 (MQ=255)                                                                   ttGTGTTTCACTGTGCCGGAACTGACCGGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCTTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTgg                                                                                  <  1:387819/150‑1 (MQ=255)                                                                     gtgtTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGaa                                                                                >  2:557440/1‑150 (MQ=255)                                                                      tttttCACTGTGCCGGAACTGACCCGGGGCGCGCTGGATGTGGCTCTCGGACACCGGGGCGTCCCTTTTCCCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGaa                                                                                <  1:116266/147‑1 (MQ=255)                                                                        tttCTCTGTGCGGGAACTGAACCGTGGCGCGTTGGATGTGGCTCTCGGACCCCGGGGCGTCCCGTTTCCTTGTATGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGaaa                                                                               <  1:442372/148‑1 (MQ=255)                                                                        tttCCCTGTGCGGGAACTGACCCGTGGCGCGGTGGATGTGGCTCCGGGCCCCGTGGGCGTACTTTTTACCTGTAGCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAg                                                                              <  2:145996/149‑1 (MQ=255)                                                                           cccggggCCGAAACTGACCCGGGGCGCGCTGGTTGTGGCTCCCGGCCCCCGGGGGGTCCCTTTTACTTGTATCGGAAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGata                                                                          <  2:891148/144‑1 (MQ=255)                                                                                tcccGAAACTAACCCGTGGCGCGCGGTATGGGGCTCTCGGACACCGGGGCGTACTTTTTACTTGTATCGGAAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGAcc                                                                     <  2:493690/148‑1 (MQ=255)                                                                                         tGACCCGTGGGGCGCTGGATGTGCCTCTCGGACTCTTGGGCGTCCCTTTTCCTTTTTTCGGGAAATTG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCAtttt                                                                <  2:268938/146‑1 (MQ=255)                                                                                           acccGTGGCGCGCGGGATGGGGCTCTCGGACACCGGGGCGTCCCGTTTCCTTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACg                                                          <  1:331729/148‑1 (MQ=255)                                                                                           aaCCGTGGCGCGCTGGATGTGGCTCTCGGACCCCTGGGGGTACCTTTTCCTTGTATCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCAc                                                           <  2:50703/149‑1 (MQ=255)                                                                                           aaCCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGc                                                         >  1:314337/1‑151 (MQ=255)                                                                                               gtggggcctgggttgtgGTTCGCGGACCCTGGGGCGTCCCTTTTTCCTGTATGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATa                                                     <  1:670011/138‑1 (MQ=255)                                                                                                 ggCGCGTGGGATGTGGCTCCGGGACCCGGGGGCTTCCCTTTTCCTTTTATGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATaaa                                                   <  1:38553/151‑1 (MQ=255)                                                                                                     cgttGGATGTGGTTCTCGGACACGTGGGGGTCCTGTTTCCCTTTACCGGAAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGAt                                                <  2:782992/147‑1 (MQ=255)                                                                                                      gtgggATGTGGCTCTCGGACACGTGGGCGTCCCTTTTCCTTGTATCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATg                                               <  1:374310/147‑1 (MQ=255)                                                                                                      gcTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGt                                              >  1:189940/1‑151 (MQ=255)                                                                                                        tGGATGTGGCTCTCGCACCCGGGGGGGTGCCTTTTCCCTGTATCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTc                                             <  1:541957/150‑1 (MQ=255)                                                                                                         ggATGTGGCTCTCGGACCCTGGGGCGTCCCTTTTCCCTGTATGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGt                                              <  1:412314/148‑1 (MQ=255)                                                                                                         ggATGTGGCTCTCGGACCCCTGGGCGTACTTTTTACTTTTATGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTcg                                            <  2:596090/150‑1 (MQ=255)                                                                                                               ggCTCCCGGACCCCGGGGCGTCCCTTTTCCTTGT‑TCGGGGAAAAGAACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGaaaa                                      <  2:757376/150‑1 (MQ=255)                                                                                                                 ctctCGGACCCCGGGGCGTACCGTTTCCCTGTATCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAg                                     <  1:719486/149‑1 (MQ=255)                                                                                                                     cGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCt                               >  2:104984/1‑151 (MQ=255)                                                                                                                     cGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCt                               >  1:295050/1‑151 (MQ=255)                                                                                                                      ggcccccgggggGTACCTTTTCCCTGTTGGGGGAAAAGG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCt                               <  1:346685/142‑1 (MQ=255)                                                                                                                         cccggggggGTCCCTTTTACCTGTATGGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGaa                            <  2:134937/145‑1 (MQ=255)                                                                                                                          acCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGa                             >  1:742591/1‑148 (MQ=255)                                                                                                                                  gtcccGTTTACCTGTATCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTaa                   <  1:890784/147‑1 (MQ=255)                                                                                                                                        tttCCCTGTATCGGGAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAAt                  <  1:252409/145‑1 (MQ=255)                                                                                                                                                tATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACt    >  1:506676/1‑151 (MQ=255)                                                                                                                                                 aTCGGACAAATG‑CCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACtt   <  1:880308/151‑1 (MQ=255)                                                                                                                                                    gggAAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTg  <  1:871855/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                   CTTTCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATG‑ACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTACTTG  >  NC_000913/436366‑436661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑