Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	50.7%	46.3 / 108.7	75	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (10/27);  new base T (38/0);  total (48/27)
Fisher's exact test for biased strand distribution p-value = 1.90e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-09

TATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTA  >  NC_000913/3206290‑3206582                                                                                                                                                     |                                                                                                                                                    tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCaaa                                                                                                                                                 >  1:865435/1‑148 (MQ=255)      cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAAt                                                                                                                                            <  2:540977/150‑1 (MQ=255)         aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCCGACaaa                                                                                                                                             >  1:1121082/1‑146 (MQ=255)          aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCCGACaaaaaaaa                                                                                                                                        >  2:896373/1‑145 (MQ=255)          aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACaaaaaaaa                                                                                                                                        >  2:432500/1‑145 (MQ=255)            aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACaaaaa                                                                                                                                     <  1:78898/151‑1 (MQ=255)              aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAaaaaaaaaaaaaaa                                                                                                                                     >  1:1093782/1‑149 (MQ=255)                tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑CTTTTTTCAGAAAAATACaaaaaacgg                                                                                                                                 >  1:799188/1‑147 (MQ=255)                     ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCCGACAAAAAAAAAAAATGGAGtt                                                                                                                             >  1:680146/1‑150 (MQ=255)                      tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTg                                                                                                                            <  1:420928/150‑1 (MQ=255)                         cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑AATTTTCCCCACAAAAAAAAAAACTTGAAGTGccc                                                                                                                         >  1:749512/1‑149 (MQ=255)                           gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACAAATAAAAAAAATGGAGTTGCCatg                                                                                                                       >  1:813580/1‑150 (MQ=255)                            ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGG‑A‑ATTTTTCAAGCAAAAAAAAAAAAcagtgttgtc                                                                                                                           >  2:416247/1‑137 (MQ=255)                            ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑AATTTTCCAGAAAAATACAAAAACTGGAGTTGGCatgat                                                                                                                     >  2:931732/1‑151 (MQ=255)                            ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGATAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑T‑A‑ATTTTTTCAGGAAAATACaaaaaattgggatgtcct                                                                                                                        >  2:601861/1‑135 (MQ=255)                                ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAtgatga                                                                                                                   <  2:176336/149‑1 (MQ=255)                                   gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCCGACAAATAAAAAAAATGGAGTTGCCATGATGAGCg                                                                                                                >  1:869427/1‑149 (MQ=255)                                     aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑T‑C‑ACTTTTTTACACCAATATAAAAAcaag                                                                                                                                 >  2:999328/1‑127 (MQ=255)                                     aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGAAAAATAAAAAAAATGGGGGTGCCCTGATGAggggaa                                                                                                             >  2:447209/1‑145 (MQ=255)                                      atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑G‑ATTTTTTCTGAAAAATAAAAAAAATGGAGTTGGCATTATGAGCgga                                                                                                              >  1:719492/1‑146 (MQ=255)                                        attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                                          <  2:1032796/150‑1 (MQ=255)                                                 tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                  <  1:118380/149‑1 (MQ=255)                                                  aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                  <  1:173958/148‑1 (MQ=255)                                                       cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAAACCAATACAAAAACTGGGGGTGCCCTGATGAGCGGAAGTAATAAACAACAAGc                                                                                             >  2:770202/1‑148 (MQ=255)                                                        gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGAAAAAAAAAAAAACAGGGGTTGCCATGATGAGCGAAAGGAATAAACAAAAAGCa                                                                                            >  1:104422/1‑148 (MQ=255)                                                          gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAAACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGAAATAAACAAAAGGccg                                                                                           >  1:1146629/1‑145 (MQ=255)                                                           ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑AATTTTTCAGAAAAAAACAAAAACTTGAGTTGGCATGATGAGGGAAAAAAATAAAAAACAAGCAggg                                                                                         >  2:587074/1‑146 (MQ=255)                                                            tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCCGAAAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGGAATAAGCAAAAGGCAGGGaa                                                                                       >  1:107852/1‑149 (MQ=255)                                                            tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                      <  2:736061/150‑1 (MQ=255)                                                                 cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACAAAAACAAAAAATGGGGGTGCCCTGATGGGCGAAAAGAATAAACAACAGGCAGTGAAAAAGtt                                                                                 >  1:1051156/1‑150 (MQ=255)                                                                 cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGAAAAATACAAAAAACGGAGATGCCCTGATGGGCGAAAATAATAAGCAAAAAGCAGGGGATaa                                                                                    >  1:10439/1‑147 (MQ=255)                                                                 cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑AATTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGGGCGAAAGGAAAAAGCAACAGGCAGGGAATAAggt                                                                                 >  1:181195/1‑148 (MQ=255)                                                                 cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGA‑G‑A‑ATTTTTTTCGAGAAATAAAAAAACAAGAGCTGCCCTGATTAGGGCAAGTAAAAAACAAAAAGCAGTGAATAAg                                                                                   >  1:279450/1‑148 (MQ=255)                                                                      aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga                                                                           <  2:733297/151‑1 (MQ=255)                                                                       cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAGGAATAAGGAACAGGCAGTGAATAAATTGACa                                                                             >  1:1129748/1‑148 (MQ=255)                                                                        tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAGGAATAAACAACAGGCAGTGAATAAGTTGACag                                                                            >  1:357157/1‑148 (MQ=255)                                                                         gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                                         <  2:99642/150‑1 (MQ=255)                                                                         gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                        <  1:115421/151‑1 (MQ=255)                                                                           tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                                       <  1:549024/150‑1 (MQ=255)                                                                             aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAA‑ATTTTTTCA‑ACAAATAAAAAAACTGGAGTTGGCATGGTGAGGGGAAGAAATAAACAACAAGCAGTGAAAAAAGTGACAGAGGTTGt                                                                     >  2:970106/1‑150 (MQ=255)                                                                             aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑AATTTTTCAGACAAATAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACAAAAAGCAGGGAATAAGTTGACAGAGATTGt                                                                     >  2:1027899/1‑150 (MQ=255)                                                                               tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCACACAAATAAAAAAAATGGAGTTGGCATGATGAGGGGAAGTAATAAAAAACAGGCAGTGAATAAGTTTACAGAGATTGTCg                                                                   >  2:75204/1‑150 (MQ=255)                                                                               tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑AATTTTTCAGACAAAAACAAAAAATGGAGTTGTCCTGATGAGGGGAAGAAATAAACAAAAAGGAGGGAATAAGGTGACAGAGATTGTCg                                                                   >  2:966597/1‑150 (MQ=255)                                                                                 cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑GAA‑ATTTTTCCA‑ACAAATAAAAAAAATGGAGTTGTCCTGATGAGCGGAAGTAAAAAACAAAAAGCAATGAATAAATTTACAGAGATTTTCGCt                                                                 >  2:86477/1‑150 (MQ=255)                                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc                                                              <  2:939116/150‑1 (MQ=255)                                                                                      tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGAAAAAAAAAAAAAATGGAGTTGGCATGATGAGGGGAAGGAATAAGAAAAAGGCAGGGAATAAGTTGACAGAGATTGTCGCTAACttt                                                           >  1:787836/1‑151 (MQ=255)                                                                                      tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                            <  2:1051106/150‑1 (MQ=255)                                                                                       cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCCGACAAATACAAAAAATTGAGTTGCCATGATGAGCGGAAGGAATAAGCAAAAGGGAGGGAATAAGGTTTCAGAGATTGTCGCTTACttt                                                           >  2:437878/1‑150 (MQ=255)                                                                                         cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAc                                                         <  2:465125/150‑1 (MQ=255)                                                                                               cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAAAGGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGcc                                                     >  1:340132/1‑148 (MQ=255)                                                                                                aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑AATTTTTCAGAAAAATAAAAAAAATTGGGTTGGCCTGATGAGCGGAAGGAATAAACAAAAAGCAGGGAATAAGTTTGAAGAGAATGTCGCTAACTTTAACGCCCTg                                                  >  2:983322/1‑150 (MQ=255)                                                                                                 aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGGTGAGGGAAAGAAATAAACAAAAAGCCGTGAATAAGGTGACAGAGATTGTCGCTAACTTTAACGCCACGAt                                                >  1:61483/1‑151 (MQ=255)                                                                                                 aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                                <  1:1106732/151‑1 (MQ=255)                                                                                                  aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCCGACAAATAAAAAAAACGGAGGTGCCATGAAGAGGGGAAGTAATAAGCAAAAGGCAGGGAATAAGTTGACAAAGATTTTTGCCAACTTTTCCCCCATGAtt                                               >  1:1081530/1‑151 (MQ=255)                                                                                                   aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                                              <  1:1174801/151‑1 (MQ=255)                                                                                                   aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGAAAAATACAAAAACTGGAGTGGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                                              <  2:276475/151‑1 (MQ=255)                                                                                                    aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTc                                             <  2:802114/151‑1 (MQ=255)                                                                                                       ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAc                                          <  1:319350/151‑1 (MQ=255)                                                                                                         aaacCCAAATTTTGAGGGGTTGTTTGGGGCGGT‑A‑A‑ATTTTTCCAGACAAAGAAAAAAGCGGGAGTAGCCATGAGAGGCGAAGTTAATAAGCAACCGGCAGTAAATAATTTGAGAGAAATGGCCGCTAACTTTACCGCCATGATTTCTAcc                                         <  2:908671/150‑1 (MQ=255)                                                                                                              cacaTTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCCTGATGAGGGAAAGGAAAAAAAAAAAAGCAGTGAAAAAGTTGAAAGAGATTGTCGCTAAATTTACCGCCCTGATTTCTAccc                                        >  2:931532/1‑146 (MQ=255)                                                                                                              cacaTTTTTAGTGGTAGTTTGTGGCGGA‑G‑ACATTTTTCC‑CACAAAATCAAAAAAAGGAGTTGCCAATAATAGCGGAAGGAATAAACAAAAGGCAGGGAATAAATTTGAAGGGAATGTTGCTTAATTTACCCCCCAGATTTTTAccc                                        >  2:902346/1‑146 (MQ=255)                                                                                                               acaTTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCCGACCAATACAAAAACTGGGGTTGGCCTGGTGAGGGGAAGTAAAAAGCAAAAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTACCCCAATGc                                  >  1:680914/1‑151 (MQ=255)                                                                                                               acaTTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATg                                   <  2:680126/150‑1 (MQ=255)                                                                                                                 aTTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACAAAAAAAAAAAATGGGGTTTGCATGATGAGGGGAAGGGAAAAGCAAAAAGGAGTGGAAAAGTTGACAGAGGGTGTGGCTAAAATTACACACATGTTTTTTACCCGaacgg                                  >  2:9314/1‑146 (MQ=255)                                                                                                                 aTTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCt                                <  1:1117259/151‑1 (MQ=255)                                                                                                                     ttAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACAAATACAAAAACTGGAGTTGGCATGATGAGCGGAAGGAATAAACAAAAAGCAGGGAATAAGATGAAAGAGATTGTTGCTAACTTTACCGCCCTGATTTTTACCCCAAAACCtgat                             >  2:1217895/1‑150 (MQ=255)                                                                                                                       aGTGTTAGTTTGGGGGGGT‑G‑A‑ATTTTTCCAAACAAATAAAAAAACTGGAGTTGCCTTGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                             <  2:865413/148‑1 (MQ=255)                                                                                                                        gTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCCGTCAAATACAAAAACTTGGGTTGCCATGGTTAGGGGAAGGAATAAACAAAAAGCCGTGAAAAAGTTGACAGAGATTGTCGCTAAAATTAACGCCATGATTTCTACCCGAAAGGCtgatg                            >  2:752611/1‑148 (MQ=255)                                                                                                                        gTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAGCAAAAAGCAGTGGATAAGATGACAGAGAATGTCGCTAACTTTTCCGCCATGATTTTTACCCGAATGCCtga                              >  1:300039/1‑146 (MQ=255)                                                                                                                            tAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtgg                      <  1:1171645/150‑1 (MQ=255)                                                                                                                            tAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAATTTAACGCCATGATTTCTACCCCAAAGCCTGATGACgtgg                      >  1:799218/1‑150 (MQ=255)                                                                                                                               tttGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggt                     <  2:871017/148‑1 (MQ=255)                                                                                                                               tttGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                    <  2:633346/149‑1 (MQ=255)                                                                                                                                           g‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc       >  1:19229/1‑151 (MQ=255)                                                                                                                                               aTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc       >  1:56074/1‑149 (MQ=255)                                                                                                                                                  ttcccAGACAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAAGCAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt   <  1:106863/147‑1 (MQ=255)                                                                                                                                                     ccAGACAAATACAAAAACTGGGGTTGCCATGAGGAGCGAAATTAATAAGCAACAGGCATTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa  <  2:815076/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑A‑ATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTA  >  NC_000913/3206290‑3206582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑