Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,846,674	0	T	G	41.2%	98.3 / 19.2	68	T247P (ACC→CCC)	hycD	hydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (10/30);  new base G (27/1);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.68e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCA  >  NC_000913/2846524‑2846823                                                                                                                                                        |                                                                                                                                                      cAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGt                                                                                                                                                       <  2:770337/151‑1 (MQ=255)   aaGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGttt                                                                                                                                                     <  2:667823/151‑1 (MQ=255)   aaGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGttt                                                                                                                                                     <  2:179814/151‑1 (MQ=255)      ccAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCa                                                                                                                                                  <  1:821392/151‑1 (MQ=255)       cAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑AGGTTcc                                                                                                                                                   >  1:1102459/1‑149 (MQ=255)        aaaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCAtt                                                                                                                                                >  2:1035495/1‑151 (MQ=255)         aaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCAttt                                                                                                                                               <  2:334025/151‑1 (MQ=255)          aCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTg                                                                                                                                              <  1:531937/151‑1 (MQ=255)            ccagcccagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGGTTTCCAtttt                                                                                                                                              >  2:1050790/1‑149 (MQ=255)             cagcccagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTTTcc                                                                                                                                           >  1:84523/1‑151 (MQ=255)              agcccagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTccc                                                                                                                                          <  1:294758/151‑1 (MQ=255)              agcccagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTGTccc                                                                                                                                          >  2:1049516/1‑151 (MQ=255)               gcccagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTTTcccc                                                                                                                                         >  1:954026/1‑151 (MQ=255)                cccagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCa                                                                                                                                        <  2:1115166/151‑1 (MQ=255)                  cagGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACg                                                                                                                                      <  2:343592/151‑1 (MQ=255)                    ggTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACgg                                                                                                                                     <  2:1070838/150‑1 (MQ=255)                     gTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCCTTTGTcccc                                                                                                                                         >  1:437794/1‑145 (MQ=255)                      tAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGGA‑GGTTTCCATTTTTCCCCCCGGaa                                                                                                                                   >  2:1110147/1‑150 (MQ=255)                        aTGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATaa                                                                                                                                <  2:336744/151‑1 (MQ=255)                            gcgGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTTCATTTTTCCCCACGGAATAAacac                                                                                                                            >  1:1102356/1‑151 (MQ=255)                             cgGAGTAATATCAAGACGCAGACGGGCCATTCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTGTCCCCACGGAATAAACAcc                                                                                                                           >  1:860575/1‑151 (MQ=255)                              ggAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGCGGA‑GGGTTCCTCTTTTACCCACGGAATAAAGAcc                                                                                                                           >  2:888526/1‑150 (MQ=255)                                  tAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTTCATTTGTGCCCACGGAAAAAAAAcccc                                                                                                                         >  2:841720/1‑148 (MQ=255)                                     tatCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTTTTCCCACGGAAAAAAAACCCccac                                                                                                                      >  2:522475/1‑148 (MQ=255)                                      atCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCACTTTTTCCCACCGAATAAACACCCCGCCGaa                                                                                                                   >  2:94910/1‑150 (MQ=255)                                        cAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCCTTTTTTCCCCCGGGATAAAAACCCCCGCGAACa                                                                                                                 >  2:227914/1‑150 (MQ=255)                                           gACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTTCATTTTTTCCCACGGAATAAACACCCCGACGAACATCTg                                                                                                             >  2:474773/1‑151 (MQ=255)                                             cGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGc                                                                                                            <  2:777291/150‑1 (MQ=255)                                             cGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGc                                                                                                            <  2:292848/150‑1 (MQ=255)                                              gCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTTCATTTGTCCCCACGGAATAAACACCCCCACGAACATCTGCa                                                                                                           >  1:473936/1‑150 (MQ=255)                                                 gACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTTCATTTTTCCCCACGGGATAAAAACCCCGACGAACATATGCAacac                                                                                                       >  2:891838/1‑151 (MQ=255)                                                     ggCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACaccacc                                                                                                   <  1:312385/151‑1 (MQ=255)                                                      gCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACAccacca                                                                                                  <  1:991559/151‑1 (MQ=255)                                                      gCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACAccacca                                                                                                  <  2:646045/151‑1 (MQ=255)                                                        cATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGc                                                                                                <  2:530505/151‑1 (MQ=255)                                                        cATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGAGAA‑GGGGTTCGATTGTTCCCACGGAAAAAAAACCCCCTTGCACATTCACAACACCACCAg                                                                                                 >  1:398761/1‑150 (MQ=255)                                                         aTGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTTTCCCCCCGGAATAAACACCCCCACGAACATCTGCAACaccacc                                                                                                   >  2:159375/1‑147 (MQ=255)                                                           gCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTGTCCCCCCGGAATAAACAACCCCACGAACATCTGCAACaccacc                                                                                                   >  1:930573/1‑145 (MQ=255)                                                            cTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCCCCAGTTTTGCGATGGCAATCACCAGCGCCAGCAGCAGTCCACCCGCGGGGAA‑GGTTTCCAGTTGTCCCCGCGGAATAAACACCCCGACGAACATCTGCAACTCCACCAGCTGt                                                                                             <  2:44825/150‑1 (MQ=255)                                                            cTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGAGTA‑GGGTTCCGTTTTTACCCACGGGATAAACACCCCCACGAAAATCTGCAACACCACCAGca                                                                                               >  1:4630/1‑147 (MQ=255)                                                               ttttCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGttt                                                                                           >  1:705752/1‑149 (MQ=255)                                                                tttCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTGTCCCCCCGGAATAAAAACCCCGACGAACATCTGCAACACCACCAGCTGttt                                                                                           >  1:1022142/1‑148 (MQ=255)                                                                 ttCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAgg                                                                                       >  1:888175/1‑151 (MQ=255)                                                                 ttCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAg                                                                                        <  1:481492/150‑1 (MQ=255)                                                                   cGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCt                                                                                     <  1:263518/151‑1 (MQ=255)                                                                    gAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTg                                                                                    <  1:920549/151‑1 (MQ=255)                                                                       cAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTTCATTTTTCCCCACGGAATAAAAACCCCGACGAACATCTGCAACACCACCAGCCGTTTCAGGCTGAt                                                                                  >  2:696367/1‑150 (MQ=255)                                                                               tAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGGA‑GGGTTTCCTTTTTCCCCCCGGAATAAACACCCCCACGAACATCTTCAACACCACCAGCTGTTTTAGGGTGATACCCCAttt                                                                         >  2:595711/1‑151 (MQ=255)                                                                                aaCCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCCTTTGTCCCCACGGAATAAAAACCCCCACGAACATCTGCAACACCCCCAGCCGTTTCAGGGTGATACCCCAttt                                                                         >  2:784531/1‑150 (MQ=255)                                                                                   cAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTGTCCCCACGGAAAAAACACCCCGACGAACATCTGCCACACCACCCGCTGTTTTAGGCTCATAACCCAatt                                                                         >  2:130060/1‑147 (MQ=255)                                                                                           cGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCaaa                                                             <  1:557925/151‑1 (MQ=255)                                                                                           cGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCaaa                                                             <  1:1125131/151‑1 (MQ=255)                                                                                              ccaccaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAgc                                                           <  2:776646/150‑1 (MQ=255)                                                                                              ccaccaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑AGTTTTCATTTTTCCCCACGGAATAAAAACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAacc                                                          >  1:432154/1‑151 (MQ=255)                                                                                                   aGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCATTTTTCCCCACGGAAAAAAAACCCCCACGAACATCTGCAACACCACCAGCCGTTTCAGGCTGATACCCCCTTTCATGACGCCAAAaccgc                                                        >  1:1028146/1‑148 (MQ=255)                                                                                                         aCGATGGCAATCCCCAGCGCCAGCAGCAGTCCCCCGGCGGTGAA‑GGTTTCCAGTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCACCACCACCAGCTGTTTCAGGCTGATACCCCATGGCATGACGCCAAAgccgctgccgct                                                 <  2:849494/149‑1 (MQ=255)                                                                                                          cGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCCTTTGTCCCCACGGGATAAACACCCCGACGAAAATCTGGCACACCACCAGCTGTTTCAGGCTGATAACCCCTTTCATGACGCCAAAGCCGCTGCCGCTGt                                               >  1:1103351/1‑150 (MQ=255)                                                                                                              ggCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCa                                          <  1:192648/151‑1 (MQ=255)                                                                                                                 aaTCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAAAACCCCCACGAACATCTGCAACACCAACAGCTGTTTCAGGCTGATACCCCCTTTCATGACGCCAAAGCCGCCGCCGCTGTAtt                                            >  1:466001/1‑146 (MQ=255)                                                                                                                    cACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCCTTTGTCCCCACGGGATAAACACCCCGACGAACATCTGCAACACCACCCCCTGTTTCAGGCTGATAACCCATTTTATGACGCCCAAGCCCCTGCCGCTGTATTCagaga                                      >  1:567706/1‑149 (MQ=255)                                                                                                                     aCCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGGTTCCCTTTGTCCCCACGGAATAAACACCCCGACGAAAATCTGCAACACAACCAGCTGATTTAGGCTGATACCCCCTTTTCTGAAGCCCAAACCGCCGCCGCTGGATTCAGAGAGc                                    >  2:15776/1‑150 (MQ=255)                                                                                                                      ccAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCgg                                  <  1:601411/151‑1 (MQ=255)                                                                                                                       cAGCGCCAGCAGCAGTCCACCGGCGGTGGA‑AGGTTCCATTTTTTCCCCCCGGATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCCCTGCCGCTGTATTTAGAGAGCggg                                 >  2:1122405/1‑151 (MQ=255)                                                                                                                                cagcagTCCACCGGCGGTGAA‑GGGTTCCATTTGACCCCACGGAATAAACTTCCCTACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTGCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGc                        <  2:625275/151‑1 (MQ=255)                                                                                                                                 agcagTCCACCGGCGGTGAA‑GGTTTCCATTTTTCCCCACGGAATAAACACCCCCACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAACCGCCGCCGCTGTATTCAGAGAGCGGGCCTTCCTGc                        >  2:962922/1‑150 (MQ=255)                                                                                                                                   cagTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGc                     <  2:1102327/151‑1 (MQ=255)                                                                                                                                            ggcggTGAA‑GGTTTCCATTTGGCCCCACGGAATAAACACCCCGACGAACATCTGCACCACCTCCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGTTCGGGCCTTCCTGCAGCTCCTGCTc             <  2:935369/150‑1 (MQ=255)                                                                                                                                             gcggTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCgg           <  1:1170492/151‑1 (MQ=255)                                                                                                                                                      ggTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGcc   <  2:507372/151‑1 (MQ=255)                                                                                                                                                       gTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCa  <  2:376099/151‑1 (MQ=255)                                                                                                                                                       gTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCa  <  1:400789/151‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                      CAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAA‑GGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCA  >  NC_000913/2846524‑2846823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑