Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	233,118	0	T	C	42.1%	86.7 / 15.6	57	I280V (ATT→GTT)	mltD	putative membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base T (31/2);  new base C (0/24);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 4.32e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTT  >  NC_000913/232969‑233259                                                                                                                                                          |                                                                                                                                              cGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑t                                                                                                                                               <  1:807184/150‑1 (MQ=255)      gTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCg                                                                                                                                          <  2:754302/150‑1 (MQ=255)                 aTTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCt                                                                                                                               >  1:586365/1‑150 (MQ=255)                       ccTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCg                                                                                                                        >  1:304206/1‑151 (MQ=255)                       ccTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCg                                                                                                                        >  1:989568/1‑151 (MQ=255)                            aGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCAtt                                                                                                                   >  2:417880/1‑151 (MQ=255)                              ccaggcatccACGCATTTTATCTGCATGTTTTTTTGGCCCCCTCCCGCCGTGCGGCCCTCTGGCGCCGAGGGGGGACCT‑TTTT‑CCCCCAGGTTTGAT‑TTTTTTCAGTTTGCGGAGGAAAT‑TCCCCGCCATATCTGCTACCTTCGCCa                                                                                                                     <  1:711162/138‑1 (MQ=255)                              caggggtttCCCGCGGTTGATCTGCTTCTTTTTTGGGCCCCTCCACGTATGGGGGGCCTCTTGCCCCCGGCGTGGCGCT‑TTTT‑CCGCCAGCTTTGAA‑TTTCTTCAGTTTGGTGAGGAAAA‑CCCCCGCCATATCTGCTACCTTCGcc                                                                                                                      <  1:494021/139‑1 (MQ=255)                               cAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTc                                                                                                                 >  2:759892/1‑150 (MQ=255)                                    aTTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCgg                                                                                                           >  2:886073/1‑151 (MQ=255)                                       cccGCTGTTGTTCTGCTTTCTTTTTTGCCCCCCCCACGTGCGGCGGCCCCCTCGCCCCCGGGGTGGACCT‑TTTC‑CCGCCAGTGTGGAT‑TTTCTTCGGTTTGCGGAGGAAAA‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGgct                                                                                                        <  2:90303/149‑1 (MQ=255)                                       cACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGgct                                                                                                        >  2:1072479/1‑151 (MQ=255)                                         cGCAGTTGCTCCGCTTGCTTCTTGGGCACCATCACGTTCGGCGGCCCTCTCGCCCCGGGGGTGG‑GCC‑TTTTCACGCACGCGGGAAT‑TTTCTACGGCTTGCTGCCGAAAA‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGgctg                                                                                                       <  2:705852/150‑1 (MQ=255)                                            aGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTc                                                                                                    >  1:406593/1‑150 (MQ=255)                                            aGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTc                                                                                                    >  1:718051/1‑150 (MQ=255)                                              ttGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAgg                                                                                                 >  1:570192/1‑151 (MQ=255)                                                gATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTg                                                                                               >  1:970100/1‑151 (MQ=255)                                                   cTGCTTGTTTTTTTGCCACCACCCCGTCCGGGGGCCCACTGCCGCCCGGCGGGGCGCT‑TTTT‑CCGCCAGGTTTTAT‑TTTTTTCAGCTTGCTGACGAAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGc                                                                                              <  2:923215/149‑1 (MQ=255)                                                     gcttgtttcttGGGCACCACCGCGTCCGGCGGCCCCCGGCCCCCCGGCGGGGAGCT‑TTTT‑CCGCCGGGTTTGAA‑TTTCTTCAGCTTGCGAAGGAAAA‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGca                                                                                             <  1:915506/142‑1 (MQ=255)                                                     gCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCacac                                                                                          >  2:646011/1‑151 (MQ=255)                                                           ttcttTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGcc                                                                                     >  1:885206/1‑150 (MQ=255)                                                           ttcttTGCCACCACCACGTTCGGCGGGCCCTTCGCCCCCGGGGTGGAGTT‑TTTC‑ACCCGGGCGTTGAT‑TGTCTTCCGCTTGCTGGGGAAAA‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACgcgc                                                                                      <  2:934805/149‑1 (MQ=255)                                                            tcttTGCCCCCACCACGTACGGGGGCCCCCTGGCCCCCGGGGGGGAGCT‑TTTT‑CCGCCAGCGTTGAT‑TGTCTCCAGCTTGCTGACGAAAA‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCa                                                                                    <  1:100720/150‑1 (MQ=255)                                                              tttGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCag                                                                                   >  1:905281/1‑149 (MQ=255)                                                                   cACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTAAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACgg                                                                            >  1:629682/1‑151 (MQ=255)                                                                      cATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCtt                                                                         >  2:353748/1‑151 (MQ=255)                                                                       caccgggtcctggggCCCCTTGCCCCCCGGCGGGGGCCTTTTT‑ACGCCGGCTTTGAT‑TTTCTTC‑GTTTTCTGGAGGAAACCCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCtt                                                                         <  1:496453/139‑1 (MQ=255)                                                                                 gCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGt                                                              >  1:712423/1‑151 (MQ=255)                                                                                 gCGGCCCCCTCGCCCCCAGGGGGGAGCT‑TTTT‑CCGCCGGGGTTGAA‑TGTCTCCAGCTTGCGGAAGAAAA‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTc                                                                <  1:309089/149‑1 (MQ=255)                                                                                 gCGGCCCACGTGCCCCGGGGGGGGACCT‑TTTT‑CCGCCAGGGTTAAT‑TCTCTCCAGCTTGCTGAGGAAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCttt                                                                        <  2:716658/141‑1 (MQ=255)                                                                                    gccctctgccgcCCACGGGGGGGCC‑TTTC‑CCGCCAGTGTTAAT‑TTTCTTCAGTTGGGGGACTGAAG‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTgg                                                           <  1:771513/142‑1 (MQ=255)                                                                                      cccctgccccccGGCGGGGCGCT‑TTTT‑CCGCCGGCTTTGAT‑TGTCTTCAGTTTGCGGACGAAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTgg                                                           <  1:240917/140‑1 (MQ=255)                                                                                      ccccgtGCCCCCGGGGTGGACCT‑TTTT‑CCGCCAGCTTTTTT‑TTTTTCCAGTTTGCGGGCGGAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCa                                                         <  2:777738/146‑1 (MQ=255)                                                                                       cACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGc                                                          >  1:818650/1‑149 (MQ=255)                                                                                          ttGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACg                                                     >  2:428503/1‑151 (MQ=255)                                                                                             cgcCCAGCGTGGTGCC‑TTTT‑CCCCGACGGTTGAA‑TGTCCTCAGCTTCCTGACAGAAA‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGt                                                    <  1:594640/149‑1 (MQ=255)                                                                                               cccGGCGTGGCGCT‑TTTT‑CCCCCGGTGTTGAT‑TGTCTTCGGTTTGCTGACGAAAA‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTAc                                                  <  1:753434/149‑1 (MQ=255)                                                                                                ccAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGcc                                               >  2:1066141/1‑151 (MQ=255)                                                                                                    cGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATa                                            >  1:659891/1‑150 (MQ=255)                                                                                                      tGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACg                                         >  2:1075526/1‑151 (MQ=255)                                                                                                              tttt‑CCGCCAGTGTTAAA‑TGTCTTCAGCTTGCTGACGAAAA‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGc                                    <  2:780527/149‑1 (MQ=255)                                                                                                               ttttccgcagtcGTTTAA‑TTTCTTCAGTTTCCAGACGAAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGc                                    <  2:548146/138‑1 (MQ=255)                                                                                                                     cgcaggtttGAATTGTTTTCGGTTTGCTGAGGAAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGtttt                              <  2:353483/143‑1 (MQ=255)                                                                                                                       cAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTg                            >  2:301261/1‑149 (MQ=255)                                                                                                                          cGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAat                       >  1:462765/1‑151 (MQ=255)                                                                                                                          cGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAat                       >  2:669006/1‑151 (MQ=255)                                                                                                                           gTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAat                       >  1:483976/1‑150 (MQ=255)                                                                                                                              gAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATc                    >  1:898851/1‑150 (MQ=255)                                                                                                                               aa‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCAc                  >  1:152855/1‑151 (MQ=255)                                                                                                                                  tttttccggctggctgCCGAAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCa                   <  1:747765/140‑1 (MQ=255)                                                                                                                                  tttctcCAGTTTGCTGACGAAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTgaga                         <  2:138150/140‑1 (MQ=255)                                                                                                                                   gTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTc                >  2:663368/1‑150 (MQ=255)                                                                                                                                    tCTTCATCTTGCTGACGAAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCa                   <  1:370433/146‑1 (MQ=255)                                                                                                                                      tccAGCGTGCGGACGAAAT‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAatat                     <  2:712400/140‑1 (MQ=255)                                                                                                                                               cTGACGAAAT‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCAt     <  1:607093/149‑1 (MQ=255)                                                                                                                                               cTGACGAAAC‑CCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCa      <  1:267621/148‑1 (MQ=255)                                                                                                                                                 gACGGAAA‑TCCCCGCCATATCTGCTCCATTCGCCATTTCAACCGGGCTGCTCAGGTGAACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCAtttt  >  2:990137/1‑150 (MQ=255)                                                                                                                                                  aCGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCAtttt  >  1:928992/1‑149 (MQ=255)                                                                                                                                                          |                                                                                                                                              CGACTGTACAGCAGCAATTTCGCCTGAAGCCAGAGATTCACGCAGTTGATCTGCATGCTTCTTTGGCACCATCACGTACTGCGGGCCACTTGCGCCCAGCGTGGAGCC‑TTTT‑ACGCCAGCGTTGAA‑TGTCTTCAGCTTGCTGACGGAAA‑TCCCCGCCATATCTGCTACCTTCGCCATTTCAACCGGGCTGCTCAGGTGCACACGCGCCAGAGCACGGCTTTCATCGGTCGTTGGCAGACGTACGCCATAACGCTTGCTGTTTTTGAGAATATCACTCAATGCCAGCATTTT  >  NC_000913/232969‑233259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑