Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,720	0	T	G	44.9%	71.5 / 12.0	49	V307G (GTA→GGA)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base T (7/20);  new base G (22/0);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 3.29e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAG  >  NC_000913/3572‑3868                                                                                                                                                      |                                                                                                                                                      ggTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTAc                                                                                                                                                     <  2:706099/151‑1 (MQ=255)          ggctccggcCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑CGGGCTGGgaaa                                                                                                                                              >  1:261602/1‑149 (MQ=255)              ccggcCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCGCG‑CGGACTGGGAAAATaaa                                                                                                                                         >  1:426670/1‑150 (MQ=255)                  cccGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGCACC‑AGGACTGGaaaaaaaaaaaaa                                                                                                                                     >  1:208616/1‑141 (MQ=255)                   ccGACCTTGTTCGCTCTGTGTGACAAGCCGGAGACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCCCG‑GGTAATGGGAAAATaaaaaaaa                                                                                                                                    >  1:83363/1‑145 (MQ=255)                    cGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGGGCG‑CGGACTGGGAAAATaaaaaaaaa                                                                                                                                   >  1:1104462/1‑144 (MQ=255)                    cGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAAct                                                                                                                                  <  1:576798/151‑1 (MQ=255)                             tCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATc                                                                                                                          <  2:1129351/150‑1 (MQ=255)                              cGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCt                                                                                                                         <  1:47507/150‑1 (MQ=255)                                   tgtgtgACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGCACG‑AGGACTGGGAAAAAAAAAGAAAATCTACAAACTGaaa                                                                                                                     >  2:165709/1‑149 (MQ=255)                                       tgACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑GGGACCGGAGAAATAAATGAAAATCTACAATCTGAAAAAtaa                                                                                                                >  1:255858/1‑148 (MQ=255)                                         aCAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACaa                                                                                                             <  2:981440/151‑1 (MQ=255)                                                ggAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACC‑AGGACCGGGAAAATAAATGAAAATCTTCAATCTGaaaaaaaaaaa                                                                                                             >  2:544694/1‑136 (MQ=255)                                                       gCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCt                                                                                              <  2:115230/151‑1 (MQ=255)                                                       gCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGGACTGGGAAAATAAATGAAAATCTACAATCTGAAAGATCACAAACAGCAGGGCA‑GCt                                                                                              >  2:1083663/1‑151 (MQ=255)                                                       gCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGGACTGGGAAAAAAAAAAAAAATCTAAAAACTTAAAAAACACAAAAAGCAGGGCA‑g                                                                                                >  1:587381/1‑149 (MQ=255)                                                           aGCGCGTTGCCGACTTGTTGGGCAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGCCCC‑AGGACTCGAGAAATAAATAAAAAACCACAAACTTTCAAAAAACAACACGCAGGTCA‑CCttt                                                                                            >  1:132907/1‑149 (MQ=255)                                                                  tGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGCAAG‑AGGACTGG‑GGACTAAAAAAAAAACTACAAAATTAAAAATAACAACGAACAGGGCA‑GATTTTCGCaa                                                                                      >  1:916834/1‑147 (MQ=255)                                                                   gCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTAATGGGAAAATAAATGAAAATCTTCAATCTTAAAGAACCCAAAGAGCAGGGCC‑GCCTTTTGCAAGcc                                                                                   >  2:573991/1‑150 (MQ=255)                                                                    ccGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGCCCG‑AGGACTTGGAAAAAAAAAAAAAATCTACAAACTGAAAAAACACAACCAGAAGGGCA‑GCTTTtcgccaacc                                                                                   >  1:263509/1‑144 (MQ=255)                                                                      gACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCCCG‑AGGACTGGAAAAATAAATGAAACTCTACAAACTGAAAAAACACAACGAGAAGGGCA‑GCTTTTCGCAAGCCgca                                                                                >  2:585407/1‑148 (MQ=255)                                                                        cTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑CGGACTGGGAAAATAAAAGAAAAACTACAAACTGAAAAAACACAACAAGCAGGGCA‑GCTTTTCGCCAGCCCTAAcc                                                                             >  2:254872/1‑151 (MQ=255)                                                                          ggTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAAcc                                                                             <  2:700420/149‑1 (MQ=255)                                                                          ggTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCa                                                                           <  2:179835/151‑1 (MQ=255)                                                                           gTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCAGG‑AGTACTGGAAAACTAAATGAAGCCCTACAATCTGAAAGATCACAACTAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAg                                                                          <  2:1109216/151‑1 (MQ=255)                                                                                gTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGtt                                                                     <  2:1037071/151‑1 (MQ=255)                                                                                 tAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGGAATGGGAAAATAAATGAAAAACTACAAACTGAAAAATCACAACGAGCAGGGCA‑GCTTTTCGCAAACCGTAAACCCggggg                                                                      >  2:859617/1‑148 (MQ=255)                                                                                     aaCTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGGAATGGGAAAATAAATGAAACTCTACAAACTGAAAGATCACAACGAGCAGGGCA‑GCTTTTCGCAAGCCGTAAACCCGGGGGTggg                                                                  >  2:468404/1‑149 (MQ=255)                                                                                       cTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGGAATGGGAAAAAAAATGAAAATCTACAAACTGAAAAATCACAACGAGCAGGGCA‑GCTTTGCGCAAGCCGGAAcccccgggggggg                                                                  >  1:855710/1‑137 (MQ=255)                                                                                        tACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCCCC‑AGGAATGGGAAAAAAAAAAAAAATATAAAAACTTAAAGATAACAAAAAACAGGGCA‑GATTTTCGCAAGCCCCAAACCCGGGGGTgggggaa                                                              >  1:580875/1‑146 (MQ=255)                                                                                         aCCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGTTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCaaa                                                              <  1:163590/149‑1 (MQ=255)                                                                                                  aTCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGCACG‑AGGAATGGGAAAAAAAATAAAACTATACAAACTGAAAAAACAAAAACAACAGGGCAGGCTTTG‑GCAAGCCGGAACCCCGGGGGTGGGGGAAAAAAAggggg                                                    >  1:486691/1‑149 (MQ=255)                                                                                                       gAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGGAATGGGAAAATAAATTAAAATCTACAAACTTAAAGAACACAAACAACAGGGCA‑GCTTTTTGCAAACCGTAACCCAGGGGGTGGGGAAAAAAAAGGGGGTGgttt                                              >  1:724362/1‑151 (MQ=255)                                                                                                            ttttGTTCATATTTGCCGGCTGGATACGGCGGGCGCGCGAAGTCTTGGAAAA‑TTAATGTAACTGCAAAAGGTGAACGATAAAAAACAGACGGTCA‑GCGCTTCGGCAGCCAGGACCCCGGGGGGGGGGAAAAATAAAGGGGCGttttttt                                           >  1:639839/1‑148 (MQ=255)                                                                                                            ttttGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAAATAAATTAAAATCTACAATCTTAAAGATCACAACGAGCCGGTCA‑GCTTTGCGCAAGCCGTAAACCAGGGGGTGGGGGAAAAACAGGGGGTGgtttttt                                           >  2:814131/1‑148 (MQ=255)                                                                                                                 ttCATATTTGCCGGCTGGATACGGCGGGGGCACG‑AGGAATTGGAAAAAAAAAAAAAATCTAAAAACTGAAAGAAAACAACGAGGAAGGAA‑GCTTTTCGCGAGCCGGAACCCAGGGGGGGGGGAAAAAAAAGGGGGGGTTTTTTTCGccc                                      >  1:282435/1‑147 (MQ=255)                                                                                                                      atTTGCCGGCTGGATACGGCGGGCGCACG‑AGGACTGGGAAAATAAAAAAAAATCTACAAACTGAAAAATCACAAAGAGCAGGGCA‑GCTTTTCGCAAGCCGCAAACCACGGGGTGGGGGAAAAACAGGGGGTGtttttttccccc                                      >  2:142012/1‑138 (MQ=255)                                                                                                                         tGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCg                             <  1:902653/150‑1 (MQ=255)                                                                                                                          gCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCgg                            <  2:54442/150‑1 (MQ=255)                                                                                                                                  gATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGcc                   <  2:946337/151‑1 (MQ=255)                                                                                                                                   aTACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGcc                   >  1:192737/1‑150 (MQ=255)                                                                                                                                       ggcgggcgCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCctgact              <  1:213680/151‑1 (MQ=255)                                                                                                                                         cgggcgCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCctgactga            <  2:578337/151‑1 (MQ=255)                                                                                                                                            gcgcACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAAt         <  2:443545/151‑1 (MQ=255)                                                                                                                                              gcACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAAtt        <  2:691784/150‑1 (MQ=255)                                                                                                                                                aCG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATtgat     >  2:498810/1‑151 (MQ=255)                                                                                                                                                aCG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATtgat     <  1:515003/151‑1 (MQ=255)                                                                                                                                                    aGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATtgatg    >  1:446100/1‑149 (MQ=255)                                                                                                                                                    aGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATgag  <  1:881341/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      GGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACG‑AGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCA‑GCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAG  >  NC_000913/3572‑3868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑