Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,617,681	0	A	C	41.3%	76.4 / 11.2	46	Y35S (TAC→TCC)	yfgD	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (22/5);  new base C (0/19);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.06e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTCGCTTAGCGAAGAGGCGCTGA  >  NC_000913/2617532‑2617827                                                                                                                                                        |                                                                                                                                                   gCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTAc                                                                                                                                                   >  2:437379/1‑151 (MQ=255)  cTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTAcc                                                                                                                                                  >  2:181803/1‑151 (MQ=255)        gCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTg                                                                                                                                               >  2:308733/1‑148 (MQ=255)               aCCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACccgc                                                                                                                                     >  2:662888/1‑151 (MQ=255)                    gATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATg                                                                                                                                >  1:36059/1‑151 (MQ=255)                                        aagaaCAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTg                                                                                                            >  2:64605/1‑151 (MQ=255)                                                     aagcagaaaaaacTCTACCATAACCCCCGCTGTAAAAAGAGCCGGGAACCGCTAAACCTGGTGAAAAAAACG‑GGCGTGG‑GGCAAAAGTGGGTCCTCTCCCTGGAGCCGCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTg                                                                                                <  2:414583/142‑1 (MQ=255)                                                     aaaCAGGAAAAAATCTCCAAAACCCCCCGCTTTTCAAAGAGCCGGAAAACGCGAACCCTGCTGAAAGAAACC‑GGCGGGGGGCCAGAGGT‑GGTGCTCTCCCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTg                                                                                                <  2:747223/150‑1 (MQ=255)                                                     aaaCAGAAAAAAATCTACCATACCCCACGCTGTTAAAGGGGCGGGAAAACGCGGACCCGGCTAAAAAAAAAC‑GGCGGGGAGCAAGAGGG‑GGTGCTCTCCCTTAAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATAc                                                                                                  <  2:262426/148‑1 (MQ=255)                                                          ggaaaaaaTCTCCCATACCCCACGCTGTTAAAAGAGCCGGAAAACGCTGAACCTGCTAAAAAAAACC‑GGCGTGGAGCAAGAAGT‑GGTGCTCTCCCTGGAGACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGAt                                                                                           <  2:349851/147‑1 (MQ=255)                                                             aaaaaCCAACCACACCCCCCGCTGTCAAAAGAGCCGGAAAACGCTGACCCGGAAAAAAAAAACC‑GGCGGGGAGCCAGAGGG‑GGTGCTCTCCCTTGAGCCACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGa                                                                                            <  1:196303/146‑1 (MQ=255)                                                               aattCTACCATACCCCCCGCTGTCAAAGGAGCGGGAAACCGCTGACCCTGCTAAAAGAAAGC‑GGCGTGGAGCAAGAGGT‑GTTCCTTTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAg                                                                                     <  2:646249/148‑1 (MQ=255)                                                               aaacccacaaaacccccCGCTGTAAAAAGAGCGGGAAACCGCTAACCCTGCGAAAAGAAACC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTCCCTTGAGCCCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAg                                                                                     <  2:365430/138‑1 (MQ=255)                                                               aaaTCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAAt                                                                                       >  2:222560/1‑149 (MQ=255)                                                                             ccACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTg                                                                        >  1:107199/1‑150 (MQ=255)                                                                                cGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATgc                                                                    >  1:137622/1‑151 (MQ=255)                                                                                cGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATgc                                                                    >  1:770251/1‑151 (MQ=255)                                                                                  cTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATgcgc                                                                  >  1:426189/1‑151 (MQ=255)                                                                                     tacAAAGGGCGGGAAACCGCTGACCCTGCAAAAAAAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTCCCTTGAAACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCa                                                                <  2:558394/148‑1 (MQ=255)                                                                                        aaagtcccGGAAACCGCTAACCTTGCAGAAAAAAAAC‑GGCGGGGAGCCAGAAGT‑GGTACTTCCCTTTGAGCCCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGaaa                                                            <  2:246520/145‑1 (MQ=255)                                                                                        aaaGACCCGGAAAACGCTAACCCGGCAGAAAAAAAAC‑GGCGGGGAGCAAGAAGT‑GGTGCTCTCCTTGGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGaaa                                                            <  2:496417/151‑1 (MQ=255)                                                                                         aaGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGaa                                                             >  2:525884/1‑149 (MQ=255)                                                                                                gggAACCGCTGACCCTGCAAAAAGAAAGC‑GGGGGGGAGCCAGAGGT‑GGTCCTCTACCTTGAGACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAagag                                                         <  1:34680/146‑1 (MQ=255)                                                                                                   aaccGCTGACCCTGCTAAAAGAAACC‑GGCGGGGAGCCAGAGGT‑GGTGCTCTCCCTTGAGACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTta                                                 <  2:122097/148‑1 (MQ=255)                                                                                                    aaCGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGa                                                       >  1:425933/1‑144 (MQ=255)                                                                                                      cGCTGAACCTGCGAAAAAAAAAC‑GGGGTGGAGCCAGAAGT‑GGTCCTTTCCCTGAAGACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATaa                                              <  2:658080/151‑1 (MQ=255)                                                                                                       gCTGACCCTGCTAAAAAAAAAC‑GGCGGGGAGCCAGAAGT‑GTTTCTCTCCCTTGAGACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTtat                                                <  2:546044/148‑1 (MQ=255)                                                                                                        cTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATaa                                              >  1:130472/1‑149 (MQ=255)                                                                                                         tGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATaa                                              >  2:93465/1‑148 (MQ=255)                                                                                                         tGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATaa                                              >  1:357883/1‑148 (MQ=255)                                                                                                            aCCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACt                                        >  2:612763/1‑151 (MQ=255)                                                                                                                       aaaaaCGGGCGGGGACCAAGAGGT‑GGTCCTCTCCTTTGAGACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCt                              <  1:236127/150‑1 (MQ=255)                                                                                                                        aaagC‑GGCGGGGAGCCAGAAGG‑GGTGCTCTCCCTTGAGACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGa                            <  2:621437/151‑1 (MQ=255)                                                                                                                        aaacC‑GGCGTGGAGCCAGAGGT‑GGTGCTCTCCCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTg                             <  2:21705/150‑1 (MQ=255)                                                                                                                        aaaaC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTg                             >  2:524007/1‑150 (MQ=255)                                                                                                                         aaaG‑GGCGGGGAGCAAGAGGT‑GGTCCTTTCCCTTGACACGCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGAc                           <  1:473004/151‑1 (MQ=255)                                                                                                                           aagggCGGGGAGCAAGATTT‑GGTCTTCTACCTGGAGACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACa                          <  1:658444/148‑1 (MQ=255)                                                                                                                            c‑GGGGTGGAGCAAGAAGT‑GGTGCTCTCCCTGGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAg                         <  2:340522/150‑1 (MQ=255)                                                                                                                                 gTGGAGCAAGAAGT‑GGTGCTCTCCTTGGAGACCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTCg                     <  2:601621/150‑1 (MQ=255)                                                                                                                                   ggAGCAAAAGGT‑GGGGTTCTCCTTGGAAACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTc                      <  2:28116/147‑1 (MQ=255)                                                                                                                                         aGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTCGCTTAGCGa             >  1:473993/1‑150 (MQ=255)                                                                                                                                            aGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTCGCTTAGCGAag           >  2:684969/1‑149 (MQ=255)                                                                                                                                             gt‑ggtgCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTCGCTTAGCGAAGAgg        >  1:427493/1‑151 (MQ=255)                                                                                                                                                  tgCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTCGCTTAGCGAAGAGGCGCt    >  2:743189/1‑151 (MQ=255)                                                                                                                                                   cctctACCTGAAGCCCCCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTCGCTTAGCGAAGAGGCGCt    <  1:571064/149‑1 (MQ=255)                                                                                                                                                       tACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTCGCTTAGCGAAGAGGCGCTGa  <  1:198319/148‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                   GCTTTAAGCCTTATACCAAGATGTAATTCGCATTAAAGGAAGAACAATGACCAAACAGGTAAAAATCTACCATAACCCACGCTGTTCAAAGAGCCGGGAAACGCTGAACCTGCTGAAAGAAAAC‑GGCGTGGAGCCAGAAGT‑GGTGCTCTACCTTGAGACACCCGCCGATGCGGCAACGCTGCGCGATTTGCTGAAAATACTGGGGATGAATAGCGCCCGTGAATTGATGCGCCAGAAAGAGGATCTTTATAAAGAACTGAACCTGGCTGACAGTTCGCTTAGCGAAGAGGCGCTGA  >  NC_000913/2617532‑2617827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑