Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	646,747	0	T	G	45.9%	91.9 / 13.9	61	T255P (ACC→CCC)	citG	2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
Reads supporting (aligned to +/- strand):  ref base T (6/27);  new base G (28/0);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 7.09e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACC  >  NC_000913/646602‑646896                                                                                                                                                   |                                                                                                                                                       atTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGaa                                                                                                                                                   <  1:733807/151‑1 (MQ=255)   ttCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑t                                                                                                                                                 <  1:679147/150‑1 (MQ=255)     ccAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGc                                                                                                                                               <  1:691050/150‑1 (MQ=255)           ttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCtt                                                                                                                                         <  2:857659/150‑1 (MQ=255)             aTCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCttttt                                                                                                                                      <  2:2540/151‑1 (MQ=255)              tCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTTGAA‑TGCCCCCcttttt                                                                                                                                     >  1:105643/1‑150 (MQ=255)               cAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑AGCCCCCCTTTTgt                                                                                                                                    >  2:766933/1‑150 (MQ=255)                aaGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTGGGA‑TGCACCCctttt                                                                                                                                      >  2:729039/1‑148 (MQ=255)                aaGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGTA‑GGCCCCCctttt                                                                                                                                      >  2:749024/1‑148 (MQ=255)                    gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAG‑TGGCCCCCTTTTTCaata                                                                                                                                >  2:441967/1‑150 (MQ=255)                     cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCTTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGTG‑TTCGAA‑TGCCCCCTTTTTGCaata                                                                                                                                <  2:282513/149‑1 (MQ=255)                     cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TACCCCCCCTTTGTaataat                                                                                                                              >  2:419644/1‑151 (MQ=255)                      ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCaat                                                                                                                                 <  1:294912/147‑1 (MQ=255)                      ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTTGAA‑TGGCCCCCTTTTTCaataa                                                                                                                               >  2:793911/1‑149 (MQ=255)                      ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCTTTTTTCaataaa                                                                                                                              >  2:294767/1‑149 (MQ=255)                       tAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATg                                                                                                                             <  2:802037/150‑1 (MQ=255)                        aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGtt                                                                                                                           <  1:759575/151‑1 (MQ=255)                          ataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGACCTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTg                                                                                                                         <  1:804410/151‑1 (MQ=255)                             atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTgcgc                                                                                                                      <  1:467184/151‑1 (MQ=255)                             atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTTAATAATGttt                                                                                                                          >  1:629169/1‑147 (MQ=255)                                aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGGCTGAGATTTCGTTCGATACACTCCCTTTCGAACTGCCGGAGATAATCGAGATCGGCGTGGG‑TTCGAA‑TGCACCCTTTTTGCAATAATGTTTGCGCCt                                                                                                                    <  1:653627/150‑1 (MQ=255)                                aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTc                                                                                                                   <  1:608234/151‑1 (MQ=255)                                aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGcccc                                                                                                                   >  1:893906/1‑149 (MQ=255)                                        gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTa                                                                                                           <  2:298213/151‑1 (MQ=255)                                          tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGCCCCccgc                                                                                                               >  1:499719/1‑145 (MQ=255)                                          tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCCGTAg                                                                                                          >  1:224902/1‑150 (MQ=255)                                          tAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAAAGTTTTCGCCTCGCGCTGTAGc                                                                                                         >  1:587552/1‑151 (MQ=255)                                           aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAg                                                                                                          <  1:425358/149‑1 (MQ=255)                                                ccAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTTGGA‑AGCCCCCCTTTTTCCATAATTTTTTCGcccccccccgttgcc                                                                                                        >  1:354248/1‑133 (MQ=255)                                                 cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTTGGA‑AGCCCCCCCTTTCCCATAAACTTTTGGCCgcgcgcgccag                                                                                                          >  2:679754/1‑138 (MQ=255)                                                      aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGCGATCGGCGGGGG‑TTGGGA‑GGGGCCCTCTTTTTAATAATATGTTTTCCCCGGCCCGTAGCCAGCGCAGGcc                                                                                              >  2:629092/1‑150 (MQ=255)                                                      aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGGA‑TGCCCCCCTTTTTCAATAAAGTTTTCTCCCCCCGCCGGAGccccacccggcac                                                                                             >  2:722498/1‑140 (MQ=255)                                                      aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAAGTGCCCCCTTTTT‑TAATAAAGTTTTCCCCTCCCGCCGTAGGCAGCCCAGGccc                                                                                             >  2:249506/1‑151 (MQ=255)                                                         gATCAGTAGGTCATCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGGCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcccacc                                                                                          <  2:337022/151‑1 (MQ=255)                                                                 ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCAcc                                                                                  <  1:817981/151‑1 (MQ=255)                                                                 ggTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGACCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCAcc                                                                                  <  1:495567/151‑1 (MQ=255)                                                                    cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGATTGGAG‑GGCCCCCTTTTT‑CAATAATTTTTTCGCCTCCCCCTGTTGGCCCCGCCGGcccccccccccccccc                                                                                >  1:740660/1‑140 (MQ=255)                                                                    cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgc                                                                                <  2:847618/150‑1 (MQ=255)                                                                       cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGa                                                                            <  1:877821/151‑1 (MQ=255)                                                                            ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCTTTTTGTAATAATATTTTTGCCCCCCGCCGTTGACAGCGCAGGCCCCCCCCTCCCCCCCCCGATGc                                                                         >  2:69061/1‑149 (MQ=255)                                                                              gccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCACCCCCCGATGCAAc                                                                      >  1:342902/1‑150 (MQ=255)                                                                                     cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAAAATTTTCGCCTCGCGCTGGAGCCCGCGCAGGCCCCCCTCGCCACCCCCCGCTGCAACGCTggga                                                               >  1:237258/1‑148 (MQ=255)                                                                                        agaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCCATAAATTTTTCGCCCCGCGCCGTAGCCCGCGCAGGGCCCCCCCCCCCCCCCGCGATGCAACGGTgg                                                                 >  1:884702/1‑145 (MQ=255)                                                                                          aTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAATGTTTTCGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGATGCAACGTTGGGATCGcc                                                          >  1:945304/1‑150 (MQ=255)                                                                                           tttCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGAGG‑GTCGCA‑TTCCCCCTCTTTTCCATAATGTTTTCGCCTCGCGCCGTAAACCGCGCCGGACCCCCCCGCCCCCGCACGCAGCAACGCTgggttcccc                                                          >  1:224265/1‑142 (MQ=255)                                                                                            ttcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTTGAA‑TGCCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCGCCACCCCCCGATGCAACGTTGGGATCGcc                                                          >  1:610625/1‑148 (MQ=255)                                                                                            ttcgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCTAA‑TTCCCCCCTTTTTCAATAAATTTTTTGCCCCCCGCCGTAGCCCGCCCCCGCGCCCCCCCCCCCCCCGCCATGCAACGTTGGGGTCGCCggt                                                       >  2:8420/1‑149 (MQ=255)                                                                                                    aTACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCCCA‑TGACCCCCTTTTTCAATAATGTTTTTGCCCCCCGCCGTAGCCAGCGCCGGGCCCCCCCCCCACCCCGCCGGGCAACGTTGGGATATCCGCTGATAGccc                                               >  2:916846/1‑150 (MQ=255)                                                                                                      acacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt                                              <  1:540766/150‑1 (MQ=255)                                                                                                          tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGGA‑ATCCCCCCTTTTCTAATCAAATTTTCGCCTCGCGCCGTTGCCAGCGCCGGCCCCCCCCCCCACCCCGCGGGGCCACGCTGGGATCGccccggctcgcccccc                                            >  2:429336/1‑134 (MQ=255)                                                                                                           cccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑GTCGAA‑TGCCCCCCTTTTTCAATAAAGTTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCACCCCGCGGGGCCACGGTGGTATCGCCGTTGATCGcc                                                >  1:255148/1‑143 (MQ=255)                                                                                                                           gagaTAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc                        <  2:420263/151‑1 (MQ=255)                                                                                                                            agaTAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc                        <  2:128868/150‑1 (MQ=255)                                                                                                                                aaTCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATg                    <  1:325817/150‑1 (MQ=255)                                                                                                                                     agaTCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt              <  2:71945/151‑1 (MQ=255)                                                                                                                                      gaTCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTTCCATAATGTTTGCGCCCCGCGCTGTAACCAGCGCCGGCCCCCCTCGCCCCCGCGCGATGCAACGTTGGGATCGCCGTTGATCGCCATCCGTAGGGGCAAGGGATCGAGGAATggccggt              >  2:584099/1‑144 (MQ=255)                                                                                                                                         cggcggGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAgg          <  1:99818/151‑1 (MQ=255)                                                                                                                                           gcggGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGAt        >  1:67939/1‑151 (MQ=255)                                                                                                                                            cggGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATc       >  1:160711/1‑151 (MQ=255)                                                                                                                                             ggggg‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCt      >  1:340605/1‑151 (MQ=255)                                                                                                                                                 g‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAcc  <  1:426686/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                       ATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGG‑TTCGAA‑TGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACC  >  NC_000913/646602‑646896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑