Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	45.2%	84.2 / 58.7	85	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/41);  new base T (38/0);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 8.45e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206287‑3206584                                                                                                                                                     |                                                                                                                                                        gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                                       <  2:575650/151‑1 (MQ=255)   gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAGAATTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAg                                                                                                                                                      <  1:546927/150‑1 (MQ=255)        tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACaaa                                                                                                                                                 >  1:936700/1‑150 (MQ=255)         cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACaaa                                                                                                                                                 >  2:405219/1‑149 (MQ=255)           cAAAACTTGGCCATTCCCTTCGCTGGCGCAATATTCATTCTTATTTTCGAGTTGATTCACGATCTGTTAATTGCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTGGTTTGTGGCGATGAATTTTTCCAGCCAAAGAC‑a                                                                                                                                            <  1:677278/151‑1 (MQ=255)           cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑a                                                                                                                                            <  1:721591/151‑1 (MQ=255)              aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCCGAAAAATAa‑aaa                                                                                                                                          >  1:256115/1‑150 (MQ=255)                 aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACt                                                                                                                                      <  2:1121075/151‑1 (MQ=255)                   tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTg                                                                                                                                     <  2:900338/150‑1 (MQ=255)                   tGCCATTCCATGATCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGTCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGAGGGGAAATTTTTTAGACaaaaaa‑aaaa                                                                                                                                         >  1:167643/1‑146 (MQ=255)                     ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATg                                                                                                                                     >  2:984659/1‑148 (MQ=255)                     ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCGGAAAAACAC‑aaaaa                                                                                                                                        >  2:337075/1‑145 (MQ=255)                      cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGt                                                                                                                                 <  1:849537/151‑1 (MQ=255)                       aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCGACAAAAAA‑AAAAACAgg                                                                                                                                    >  2:284107/1‑147 (MQ=255)                       aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCCGACAaaaaa‑aaaaaaaggggt                                                                                                                                 >  2:965503/1‑143 (MQ=255)                        ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGtt                                                                                                                                <  1:1123441/150‑1 (MQ=255)                         tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTg                                                                                                                               <  2:71157/150‑1 (MQ=255)                          cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGTTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTg                                                                                                                               <  1:116352/149‑1 (MQ=255)                           ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCACACAAAAAT‑aaaaaacag                                                                                                                                    >  1:232826/1‑139 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATTGAggt                                                                                                                                >  2:758572/1‑142 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAACCTTGAGTTGCCag                                                                                                                           <  1:1002909/149‑2 (MQ=255)                               ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAA‑AAAAAATGGAGTTggcct                                                                                                                           >  1:972122/1‑144 (MQ=255)                                tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTTACACAAAAAC‑AAAAAATg                                                                                                                                     >  2:1104269/1‑137 (MQ=255)                                 ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCatgat                                                                                                                        <  2:194670/149‑1 (MQ=255)                                  tGGGGCAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTCCATATTCACGGGGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAACCGGGAGTTCCCttgat                                                                                                                        <  1:728944/148‑1 (MQ=255)                                   gtcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGc                                                                                                                    <  1:857704/149‑1 (MQ=255)                                   ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAg                                                                                                                     <  1:793599/150‑1 (MQ=255)                                     cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                  <  1:833556/151‑1 (MQ=255)                                      gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGAAAAATAA‑AAAAAATGGAGTTTGCatgat                                                                                                                        >  1:921829/1‑144 (MQ=255)                                      gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGaa                                                                                                                 <  2:262374/151‑1 (MQ=255)                                       cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGCTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGATGATTTTTTTAAAAACATAC‑AAAAAATAGGGTTGCTACGTTGAGCGaa                                                                                                                 >  1:919504/1‑150 (MQ=255)                                            ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCACAAAAATAC‑AAAAACTGGAGTTGGCATGATGAGCggaaggaa                                                                                                            >  1:727846/1‑150 (MQ=255)                                             tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                           <  2:6598/150‑1 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCaa                                                                                                    <  2:585501/149‑1 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTATCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCa                                                                                                     <  1:428772/148‑1 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAAAAAAAAACAAAAAAATGGGGTT‑CCCTGATGAGAGAAAAgaat‑aaaaaa                                                                                                    >  1:582051/1‑145 (MQ=255)                                                     aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACa                                                                                                  <  2:709521/150‑1 (MQ=255)                                                       tttCGCGTTGATTCACGAACTGTTAATTACATATTCATGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATGGAGGTGCCATGATGAGCGGAAAGAAT‑AAACaaaaag                                                                                                >  2:771812/1‑146 (MQ=255)                                                       tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATGGGGATGCCATTAAGAGGGGAAGTAAT‑AAGaaaaa                                                                                                  >  2:778249/1‑146 (MQ=255)                                                       tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAgg                                                                                                <  2:883222/150‑1 (MQ=255)                                                        ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGc                                                                                               <  1:419500/150‑1 (MQ=255)                                                         tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCa                                                                                              <  2:44918/150‑1 (MQ=255)                                                          cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCa                                                                                              <  1:657139/149‑1 (MQ=255)                                                          cgcgTTGATTCACGAACTGTTAATTACATATTCACGGAGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTGCAAAATAC‑AAAAAATGTAGTTGTCCCGAAGAGCGAAAGaaaa‑aaaaaaaa                                                                                                  >  1:949061/1‑139 (MQ=255)                                                               tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTCCACACAAATAA‑AAAAAACGGGTTGGTTATGATTATCGATAGtaat‑aaaaaaaaagaa                                                                                              >  1:935246/1‑134 (MQ=255)                                                                   tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAGAAAAATAA‑AAAAACTGGAGTTGGCATGGTGAACGGAAATAAA‑AAAAAAAAGGGAGGGAATaa                                                                                      >  2:393874/1‑148 (MQ=255)                                                                    cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGtt                                                                                   <  2:1030941/150‑1 (MQ=255)                                                                       gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACa                                                                               <  1:510749/151‑1 (MQ=255)                                                                        aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAAC‑AAAAAATGGAGGTGGCCTGATGAGCGAAAGAAAA‑AAACAAAAAGCAGGGAATaa                                                                                      >  2:1066231/1‑143 (MQ=255)                                                                            gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACagag                                                                            <  1:641792/149‑1 (MQ=255)                                                                               aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                        <  1:906186/150‑1 (MQ=255)                                                                               aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                        <  2:313907/150‑1 (MQ=255)                                                                               aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                       <  1:224354/151‑1 (MQ=255)                                                                                   aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGCGGTAGTTTGTGGCGGCGAAAATTTTCAAAAAAACAC‑AAAAACAGGAGTTGGCATTAGGAGGGGAAGGAAT‑AAACAACAACCAGTGAAAAAAATTACAAAGAAAGTCGCt                                                                   >  1:1023481/1‑151 (MQ=255)                                                                                    cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                                  <  2:1000438/151‑1 (MQ=255)                                                                                        ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCACACAAAAAA‑AAAAACTGGAGTTTCCCCGGTGAGCGGAAATAAT‑AAACAACCGGCAGTGGAAAAATTTACAGAGACTTTCGCt                                                                   >  1:340552/1‑146 (MQ=255)                                                                                          cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                            <  1:222252/151‑1 (MQ=255)                                                                                            cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                                          <  2:232820/151‑1 (MQ=255)                                                                                            cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAATAA‑AAAAAATGGAGTGTCCATGATGAGCGAAAGGAAT‑AAACAACAAGGAGAGAATAAATTGACAGAGATTTTCGCTaa                                                                 >  1:1133983/1‑144 (MQ=255)                                                                                             ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGTATTTTTTCAAATAAATAA‑AAAAAATTGAGTTGCCGAGATGAGCGAAAGCAATAAAGCAACA‑GCAGAGAATAAGCTGGACGAAATTGTGGGTAACCTTAcc                                                          >  2:172514/1‑150 (MQ=255)                                                                                              gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAA‑AAAAAAATGAGTTGGCATGATGAGGGAAAGGAAT‑AAACAACAAGCAGTGGATAAGATGACAGAGATTTTCGCTAAATTTACCGc                                                        >  2:444475/1‑151 (MQ=255)                                                                                               tGGCAAAAAATATAAAACCACATTTTTAGCGGTAGTTTGTGGCGGAGAAATTTTTCAGACAAACAC‑AAAAACTGGCGGTGGCCCGGTGAGGGGAAGAAAA‑AAACAACAAACAGTGAACAAATTGAAACCGATTGTCCGTTACttt                                                             >  1:1070565/1‑145 (MQ=255)                                                                                                  cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAATTA‑AAAAAATGGAGTTGGCATTATGTGGGAAAGTAAT‑AAACAAAAAGCCGGGAAAAAGTTGTAAGAGATTGTTGCTAAATTTTTCGcc                                                       >  2:1099830/1‑148 (MQ=255)                                                                                                   aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTAAAAACAATAC‑AAAAAATGGAGTTGTCATGAGGAGCGGAAATAAA‑AAACAAAAAACAGAGAATATGATGAGAGAGATTGTTGCTTACATTATAGCCacg                                                    >  1:145875/1‑148 (MQ=255)                                                                                                      aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAGCAAAAAAC‑AAAAAAAGGGGGTGCCTTTTTAAGGGGAAATAAA‑AAACAACAAGTAGGGGGTAAATTGACACAGAATTTTGCGAAATTTACCCCCATGAttt                                                >  2:199730/1‑151 (MQ=255)                                                                                                      aaaTAAAAAACCAAATTTTTAGGGGTATTTTGGGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCATGAGGAGCAAAAGTAAT‑AAGCAACAGGCATTAAATAGGTTGAAAGAGTTTGTCGCTAACTTTACCGCCATGAttt                                                <  1:205039/151‑1 (MQ=255)                                                                                                       aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACGGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTc                                               <  2:944729/151‑1 (MQ=255)                                                                                                        atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCCAACAAATAA‑AAAAACAGGGGTTGTCCTGATGAGTGGAAGTAAT‑AAAAAAAAAGCAGGGAAAAAATTTACAGAGAATGTCGCTTACTTTTCCGCCATGAttttt                                              >  1:1119104/1‑149 (MQ=255)                                                                                                         tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAACAGGAGTTGGCATGATGAGCGAAAGAAAT‑AAAAAAAAGGCAGGGAAAAAATTGACAGAGATTGTCGCTAACTTTACCGCCCTGAttttt                                              >  2:586296/1‑148 (MQ=255)                                                                                                           taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACCAATAC‑AAAAAATTGAGTTTCCCTGATGAGGGAAAATAAT‑AAAAAACACGGAGAGGATAAGGTTAGAGAAAATTTTGCTAAATTTTTCGCCCTGAtttttttc                                           >  2:350854/1‑146 (MQ=255)                                                                                                            aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAA‑AAAAAATGGAGTTGCCATGATGAGCGAAAAAAAA‑AAACAAAAAGCAGGGAATAAGATGACAGAGATTGTCGCTAAATTTAACGCCATGATTTTTAccc                                          >  2:782451/1‑151 (MQ=255)                                                                                                             aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTGGAGTTGCCCTGATGAGCGAAAGTAAT‑AAACAACAAGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTAccc                                          >  2:890041/1‑150 (MQ=255)                                                                                                               aCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAA‑AAAAAATGGAGGTGCCATGATGGGGGAAAGGAAT‑AAACAACAGGCAGGGAATAAATTGACAGAGATTGTTGCTAAATTTACCGCCCTGATTTTTAccc                                          >  2:913539/1‑148 (MQ=255)                                                                                                                  acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTACCAAATAC‑AAAAAATGGAGTTTCCCTGATGAGGGGAAAAAAT‑AAAACACAGGCCATAGAAAAATTGTCAGAGAATGTCGCTTAATTTTTCGCCACTATTTCTattccgaatc                                    >  1:533549/1‑142 (MQ=255)                                                                                                                  acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAC‑AAAAAATTGAGTTTCCCCGATGAGCGGAAGTAAT‑AAGCAACAGGCAGGGAATAAATTGACCGAAGTTGGCGCTAACTTTTCCGCCATGATTTCTACCCGaa                                       >  2:332736/1‑148 (MQ=255)                                                                                                                    aTTTTTTGTGGTAGTTTGTGGCGGTGAATTTTTCAAAACAAATAC‑AAAACGTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGTCAGTGATAAAGTTGAAAGAGATTTTGGTTACTTTTACCGCCATGATTTCTACCCGAATGc                                    <  1:1041664/149‑1 (MQ=255)                                                                                                                             gttattttGTGGCGGGGAATTTTTCAAAAAAAAAAA‑AAAAACGTGAGTGCCCTTGAGGAGCAAAAAAAAT‑ACGCACCAGCCATAGAATATGTTGACAAAGATTGTGGCTACCTGTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                         <  1:305714/146‑1 (MQ=255)                                                                                                                               tAGTTTGTGGCGTTGAATTTTTCCAAAAAAATAC‑AAAAACGGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtgg                        <  2:688765/150‑1 (MQ=255)                                                                                                                                   ttGTGCCGGAGAATTTTTACAGAAAAAAAC‑AAAATCGGGATTTGCCAGGATAAGCAAAAGCAAT‑AAGCACCAGGCAGTGAATAGGTTTACAGAGATTGTCGCTACCTTTGCCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                      <  1:336958/148‑1 (MQ=255)                                                                                                                                     gtgGCGGTGAATTTTTCCAGACAAATAC‑AAAAACGGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                  <  2:768192/150‑1 (MQ=255)                                                                                                                                       ggCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAA‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa                <  2:487283/150‑1 (MQ=255)                                                                                                                                         cGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt              >  2:78073/1‑150 (MQ=255)                                                                                                                                          ggTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa             >  2:157760/1‑150 (MQ=255)                                                                                                                                           gTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa            >  2:580573/1‑150 (MQ=255)                                                                                                                                                   ttCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa    >  1:274104/1‑150 (MQ=255)                                                                                                                                                     ccAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  2:582365/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                        GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCATGATGAGCGAAAGTAAT‑AAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206287‑3206584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑