Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,322,472	0	C	A	40.0%	60.9 / 11.7	40	E159* (GAA→TAA)	trpE	component I of anthranilate synthase
Reads supporting (aligned to +/- strand):  ref base C (16/8);  new base A (0/16);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 1.22e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGG  >  NC_000913/1322322‑1322619                                                                                                                                                        |                                                                                                                                                    tGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTc                                                                                                                                                     >  2:352605/1‑151 (MQ=255)       gttgttTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATcc                                                                                                                                               >  1:844567/1‑151 (MQ=255)         tgttTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATccc                                                                                                                                              <  1:87556/150‑1 (MQ=255)          gttTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGc                                                                                                                                            <  1:470816/151‑1 (MQ=255)              ttcttcttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCcac                                                                                                                                         >  1:145008/1‑150 (MQ=255)               tcttcttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCcaca                                                                                                                                        >  1:53604/1‑150 (MQ=255)                     tcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCAt                                                                                                                                 >  1:427766/1‑151 (MQ=255)                                aaaCAGGCGGGCCTGAATACGGGTGCTTTTTTTCTGATGGTAAATCCCCACCAGCGTTTCAGCGAAATAAAAACAGAAACCAGGGCAGTTATTTTCCGCTGACAGTTGCGGAAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAg                                                                                                                       <  1:620113/150‑1 (MQ=255)                                               aaTACGGGTGTTTTTTTTCTGATGTTCAACCCCCATCAGCGTTTCAGCGAAATAAAAACAAAAATCAGGGCATTTTTTTCCCGCTGACAGTTGCGATAAAT‑CTTCAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATg                                                                                                       <  1:360056/151‑1 (MQ=255)                                                    gggTGCTTTTTTTCGGATGATAACTCCCCATCGGCTTTTCAGCGAAAAAAAACCAAAAACGAGGGAGTTTATTTTCCGGTGAAATTTGCGTTAAAT‑TTCAAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCtt                                                                                                   <  1:250748/150‑1 (MQ=255)                                                             ttttGTGAGGTTCAATCCCCATCGACTTTTCAGGGAAAAAAAAACAAAAACCAGGTCATTTATGTTCCGTTAACATTTGCGTAAAAC‑CTTCAACCCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGttctt                                                                                          <  2:236465/150‑1 (MQ=255)                                                              tttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcc                                                                                        >  2:389038/1‑151 (MQ=255)                                                                     ggttCAATCACCACCAGCTTTCCAGAGATATAAAAAAAGAAATGGGGGCAGTTATTTCCCGCTGGCATTGGGAGTAAAT‑TTAAAACTCCCGCCACAATGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTa                                                                                 <  2:990416/148‑1 (MQ=255)                                                                      ggTCAACCCCCATCAGCTTTTCAGCGAGAAAAAAACAAAAATCAGGGCATTTATTTCCCGCGGACAGTTGCGGTAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcctt                                                                                      <  1:1019729/145‑1 (MQ=255)                                                                         cAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACAtt                                                                             >  1:785278/1‑151 (MQ=255)                                                                          aaTCCCCATCGGCTTTTCAGGGAAAAAAAAACAAAAACCGGGGAATTTATTTCCCGCTGACATTTGGGGAAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACa                                                                               <  1:581337/148‑1 (MQ=255)                                                                          aaTCCCCATCAGCTTTTCAGCGAAAAAAAAACAGAAATCAGGGCATTTTTTTCCCGCTGACAGTGGCGGAAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACAt                                                                              <  1:449862/149‑1 (MQ=255)                                                                               ccaacggctttcaaGCGGGAAAAAAACAGAAAGCAGGGCATTTTTTTTCCGATGACGGTTGCGGAAACT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAg                                                                       <  2:483992/138‑1 (MQ=255)                                                                                      cGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGAAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGc                                                                >  2:43389/1‑151 (MQ=255)                                                                                        tttCAGCGAAAAAAAAAAAAAAACCAGGGCATTTTTTTCCCGCTGACATTTGGGGAAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCa                                                               <  1:129631/150‑1 (MQ=255)                                                                                         ttCAGTGATAAAAAAACAGAAATCGGGGCAGTTTTTTTCCGATGACATTTGCGTTAAAT‑CTTCAACCCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCaa                                                              <  2:226422/150‑1 (MQ=255)                                                                                           cAGAGAGAAAAAAACAAAAATCGGGGAATTTATTTCCCGCTGGCAGGTGCGGAAAAC‑CTACAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATaa                                                           <  2:177568/151‑1 (MQ=255)                                                                                                    aaaaaCAGAAATCGGGGAAGTTTTTTTCCGCTGACTGTGGGGGTAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGaaa                                                    <  1:48701/149‑1 (MQ=255)                                                                                                    aaaaaCAGAAATCAGGGCATTTTTTTCCCGCTGACAGTTGCGGTAAAT‑CTTCAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGaaa                                                    <  1:950796/149‑1 (MQ=255)                                                                                                    aaaaaCAGAAATCAGGGCATTTATTCTCCGTTGACAGTTGCGGTAAAT‑CTAAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAg                                                   <  2:562926/150‑1 (MQ=255)                                                                                                     aaaaCAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCg                                                 >  1:322733/1‑151 (MQ=255)                                                                                                      aaaCAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGt                                                >  1:72538/1‑151 (MQ=255)                                                                                                          aGAAATCAGGGCAGTTTTTTCCCGCTGACAGTTGCGGAAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCaa                                             <  1:859826/150‑1 (MQ=255)                                                                                                                  gggCATTTATTTCCCGCGGACAGTTGCGGAAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGaaa                                    <  1:1041658/151‑1 (MQ=255)                                                                                                                  gggCAGTTTTTTTCCGGTGAGGGTGGCGGAAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGaaa                                    <  1:734711/151‑1 (MQ=255)                                                                                                                      aGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAggg                                 >  2:1052594/1‑150 (MQ=255)                                                                                                                        ttATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGa                                >  2:904676/1‑149 (MQ=255)                                                                                                                          aTTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCAt                            >  2:322903/1‑151 (MQ=255)                                                                                                                           tttcCCGTTAACAGTGGCGATAAAT‑CTCAAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCa                             <  2:613405/149‑1 (MQ=255)                                                                                                                              tCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGc                        >  2:210394/1‑151 (MQ=255)                                                                                                                               ccGCTGACAGTTGCGGAAAAT‑CTTAAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGc                        <  1:440548/150‑1 (MQ=255)                                                                                                                                   ctgcaagtggcgTAAATCCTTAAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCggg                     <  2:668725/139‑1 (MQ=255)                                                                                                                                     aCAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTc                 >  1:543336/1‑151 (MQ=255)                                                                                                                                        gtggCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCt                <  1:340144/146‑1 (MQ=255)                                                                                                                                         ttGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTc              >  1:716528/1‑150 (MQ=255)                                                                                                                                                      ttCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTgg  >  1:313238/1‑150 (MQ=255)                                                                                                                                                        |                                                                                                                                                    TGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAAT‑CTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTCAACAGATTCTGCAATAAACGGAAAGCGTCAAAAACCGAAAGGGAGCATAAGCGGGCGTCTTCATCCAGCAGTGG  >  NC_000913/1322322‑1322619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑