Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	42.5%	110.4 / 62.2	94	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/49);  new base T (40/0);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 2.12e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206291‑3206583                                                                                                                                                  |                                                                                                                                                      aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCAGTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACa                                                                                                                                                 <  1:872697/151‑1 (MQ=255)     cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAAt                                                                                                                                              <  2:217751/150‑1 (MQ=255)     cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATa                                                                                                                                             <  1:1168954/151‑1 (MQ=255)       cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACa                                                                                                                                           <  1:537439/151‑1 (MQ=255)        aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaa                                                                                                                                          <  2:1038764/151‑1 (MQ=255)        aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaa                                                                                                                                          <  1:1184526/151‑1 (MQ=255)          aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGAAAAAAACaaa                                                                                                                                         >  1:1340065/1‑150 (MQ=255)            cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAAc                                                                                                                                      <  2:854836/151‑1 (MQ=255)            cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AAATTTTTCAGAcaaaaacaaaaa                                                                                                                                       >  1:514279/1‑150 (MQ=255)             aTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAaaaaaaaaaa                                                                                                                                       >  2:346173/1‑149 (MQ=255)                gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                                  <  2:710933/151‑1 (MQ=255)                 ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAaaaaaaaaaa                                                                                                                                       >  2:392886/1‑145 (MQ=255)                 ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAATACAAAAAATg                                                                                                                                    >  1:122020/1‑148 (MQ=255)                  cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAACAAAAAATgg                                                                                                                                   >  2:855750/1‑148 (MQ=255)                  cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGt                                                                                                                                <  2:908297/151‑1 (MQ=255)                   aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGAaaaaaaaaaaaa                                                                                                                                       >  1:1295827/1‑143 (MQ=255)                    ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCATACAAATACAAAAAATGGAGtt                                                                                                                               >  1:40679/1‑150 (MQ=255)                    ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATAAAAAAAATagaggt                                                                                                                               >  2:1217453/1‑148 (MQ=255)                     tCCCTGCTCTGGCGCAATATTCATTCTTATTTACGCGTTAATTCAAGAACTGTTAATTACATATGCACGGTGGCAAAAAATATAAACCCGCATTTTTAGAGGTAGTTTGTGGCGGTT‑AAGTTTTTTTAACAAATAAAAAAAAAGGAggagg                                                                                                                             >  1:1393544/1‑147 (MQ=255)                       ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTATAAAAATAAAAAAAATGGAGGTgtc                                                                                                                            >  1:715086/1‑148 (MQ=255)                          gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGCTTCACGCACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑AATTTTTTCATAAACAACCAAAAAATggcgggt                                                                                                                              >  1:109336/1‑140 (MQ=255)                          gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat‑g                                                                                                                        <  1:1395346/150‑1 (MQ=255)                             ctGACGCAATAATCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATGTCTAGTGGTAGTTTGTCGCGGTG‑AATTTTTCCAGACAAATGCAAAAACTGGAGTTGCCat‑tat                                                                                                                      <  1:1056260/149‑1 (MQ=255)                              tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑AATTTTTTTCAACAAATACaaaaaattggggtgcc                                                                                                                            >  2:993218/1‑132 (MQ=255)                               ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGc                                                                                                                  <  2:1092515/151‑1 (MQ=255)                               ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGc                                                                                                                  <  1:1166873/151‑1 (MQ=255)                                 cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGa                                                                                                                <  2:368384/151‑1 (MQ=255)                                 cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGa                                                                                                                <  2:720533/151‑1 (MQ=255)                                  gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGaa                                                                                                               <  2:427130/151‑1 (MQ=255)                                   cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAATAAAAAAAACGGAGGTGCCAT‑GATGAGCGaa                                                                                                               >  2:176867/1‑150 (MQ=255)                                     atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGt                                                                                                            <  1:988981/151‑1 (MQ=255)                                      tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAAAAAAAAAATGGGGTGGCAT‑GATGAGCGgaa                                                                                                              >  2:746794/1‑148 (MQ=255)                                      tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAAATTTTTCACGAAAAAAAAAAAAAC‑AGAGTTGGCCT‑GGTGAGGGGAAGt                                                                                                            >  1:653363/1‑150 (MQ=255)                                       attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGtaa                                                                                                          <  1:492460/151‑1 (MQ=255)                                          cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCTAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGtaataa                                                                                                       <  2:474754/151‑1 (MQ=255)                                          cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGtaataa                                                                                                       <  1:1264807/151‑1 (MQ=255)                                           attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGtaataa                                                                                                       <  1:62296/150‑1 (MQ=255)                                               ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AATTTTTTCCGAAAAAAAAAAAAAATGGGGGTGGCCT‑GATGAGGGGAAGGAATAAACaa                                                                                                   >  2:1201359/1‑150 (MQ=255)                                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAATACAAAAAATGGAGGTGGCAT‑TATGTGGGGCAGGAATAAACaa                                                                                                   >  2:537609/1‑149 (MQ=255)                                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCaa                                                                                                   <  2:1255587/149‑1 (MQ=255)                                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCa                                                                                                    <  1:941741/148‑1 (MQ=255)                                                 aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGTGAAAGTAATAAGCAACAg                                                                                                <  2:631719/151‑1 (MQ=255)                                                 aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACa                                                                                                 <  2:869404/150‑1 (MQ=255)                                                 aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACa                                                                                                 <  2:330031/150‑1 (MQ=255)                                                 aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTCAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAg                                                                                                <  2:692639/151‑1 (MQ=255)                                                  ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAATAAAAAAAATGGAGGTGTCCT‑GATGAGCGGAAGAAATAAACaa                                                                                                   >  2:191903/1‑147 (MQ=255)                                                   tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAgg                                                                                               <  1:779984/150‑1 (MQ=255)                                                   tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAgg                                                                                               <  1:644100/150‑1 (MQ=255)                                                     tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATAAAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAacaacaag                                                                                               >  2:1053058/1‑146 (MQ=255)                                                       gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                                          <  1:265940/151‑1 (MQ=255)                                                         gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                        <  2:558378/151‑1 (MQ=255)                                                         gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGa                                                                                         <  1:49095/150‑1 (MQ=255)                                                           tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGAAAAAAACAAAAAATGGAGGTGGCAT‑GATGAGAGAAAGAAATAAGAAAAAAGCAg                                                                                            >  1:242281/1‑145 (MQ=255)                                                            gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAATATAAAAAATGGAGTTTGCAT‑GATGAGCGAAAGAAATAAGCAAAAAGCAGAGaaaa                                                                                      >  1:599626/1‑148 (MQ=255)                                                               tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAAAACAAAAAATGGGGGTGCCCT‑GAAGAGGGAAAGTAATAAACAACAGGCAGGGAaaaa                                                                                     >  2:1402142/1‑148 (MQ=255)                                                               tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGt                                                                                   <  1:351456/150‑1 (MQ=255)                                                                cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                                  <  1:1294095/150‑1 (MQ=255)                                                                   gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAACAAAACAAAAACTGGGGTTGGCAT‑TATGAGAGAAAGTAATAAAAAAAAAGCAGGGAAAAAgattac                                                                               >  1:234703/1‑145 (MQ=255)                                                                    aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACag                                                                             <  1:813562/151‑1 (MQ=255)                                                                      cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAATACAAAAAATAGGGTTGGCAT‑GATGAGCGAAAGTAAAAAACAACAAGCAGTGAATAAAtttaaa                                                                              >  1:449847/1‑144 (MQ=255)                                                                       tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGCCAAATACAAAAAATAGAGTTTCCAT‑GATGAGCTAAAGTAATAAGAAACAAGccgggtattaaat                                                                                  >  1:301315/1‑131 (MQ=255)                                                                           aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                      <  1:275662/151‑1 (MQ=255)                                                                             ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt                                                                      <  1:1315219/149‑1 (MQ=255)                                                                              tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCAT‑GATGAGCGGAAGGAATAAACAAAAGGCAGGGAATAAGTTGACAGAGATTGTCg                                                                    >  2:851969/1‑150 (MQ=255)                                                                                cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                                 <  2:337227/151‑1 (MQ=255)                                                                                   atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑TATTTTTTCTGAAAAAAAAAAAAAATGGGGGTGGCGA‑TATTAGCGGAAGTAATAAGCAAAAAGCAGGGAAAAAGTTGACAGAGATTGTCGc                                                                   >  2:358911/1‑146 (MQ=255)                                                                                      cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAACAAAAAATGGGGTTGCCCC‑GATGAGCGGAAATAATAAACAACAGGCAGTGAATAAGATGACAGAGAGTGTTGCTAACctt                                                            >  2:1296026/1‑150 (MQ=255)                                                                                      cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAAAAAAAAAAAAAGGGTTGGCAT‑GATGAGGGAAAAGAAAAAAAAAAAAGGAGGGGAAAAGTTGACAGAGATTGTCGCTAACttt                                                            >  1:301048/1‑150 (MQ=255)                                                                                                     tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCAT‑GATGAGCGAAAGAAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAAATTTACCGCCATGAttttt                                             >  2:1175616/1‑148 (MQ=255)                                                                                                         aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCCGACAAATATAAAAAATGGGGTTGGCAT‑GTTGGGGGAAAAGAATAAAAAAAAAGAAGTGAAAAAGATGACAGAGAATGTTGCGAAACTTTCCGCCCAGATTTTTAcccc                                        >  1:1231613/1‑150 (MQ=255)                                                                                                           aCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGAACAATACAAAAAATTGAGTTGGCCT‑GATGAGGGAAAGGAAAAAGCAACAGGCAAGGAAAAAGTTGAAAGAGATTTTTGCTAAATTTACCGCCATGATTTTTTccc                                         >  1:1245724/1‑148 (MQ=255)                                                                                                            ccACATTTTTAGTGGTATTTTGTGGCGGTG‑AATTTTCCCAAACAAATACAAAAACTGGATTTGCCAT‑GATGGGCAAAAGTAATAGGCAACGGGCAGTGAATAAGTGGAAAAAAATTGTCCTTAATTTTACCGCCATGATTTCTACCCGAAt                                     <  2:1398752/151‑1 (MQ=255)                                                                                                            ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGCCCAAAATAAAAAATTGAGTTGGCATGGGGGGGGGAAAGAAAAAAAAAAAAGG‑AGGGGAAAAGTTTAGAGAGATTGTCGCTAACTTTTTCCCCCTGATTTTTTccc                                         >  1:1368689/1‑147 (MQ=255)                                                                                                            ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                      <  2:718957/150‑1 (MQ=255)                                                                                                            ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAATACAAAAACTGGAGGTGCCAT‑GATGAGCGAAAGTAATAAACAAAAAGGAGCGGAAAAATTGACAGAGATTGTCGCTAACTTTACCGCCACGATTTCTACCCCaa                                      >  2:1236893/1‑150 (MQ=255)                                                                                                            ccACATTTTTAGTGGTAGTTTGTGGCGGTA‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTATTTTTACCGCCATGATTTCTACCCGAAt                                     <  1:1342992/151‑1 (MQ=255)                                                                                                              acttttttAGTGGTAGTTTTTGGGGTTG‑AATTTTTCCAGAAAAATAAAAAAACTTAATTTGCCAT‑GATGGGCAAAATTAAAAGGCACCTGGCAGTGAATATGTTGACAGATCTGGCTCCTATCTTTACCGCCATGATTTCTACCCGAAt                                     <  1:766171/146‑1 (MQ=255)                                                                                                                aTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGAGTTGTCAT‑GATGAGCGGAAGAAATAAACAAAAAGCAGAGAAAAAGTTGACAGAGATTGTCGCTAAATTTTTCGCCCTGATTTCTACCCGCATGcc                                  >  2:917654/1‑150 (MQ=255)                                                                                                                aTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATAAAAAAACTGGGGTTGCCAT‑GATGGGGGAAAATAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTTGCTAACTTTTTCGCCCTGATTTCTAACCCAAAGcc                                  >  2:1411066/1‑150 (MQ=255)                                                                                                                 tttttAGTGGTAGTTTGTGGCGGGG‑AAATTTTTCAGAAAAATTTAAAAAATGGAGGTGGCCT‑GGTTAGGGGAAGTAATAAAAAAAAGGAAGAGAATAAAATGACAGAGATTGTTGCTAACTTTAACCCCCTGATTTTTACCCGaa                                      >  1:1026652/1‑145 (MQ=255)                                                                                                                  ttttAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATTGAGGTTGCAT‑GATGAGCGAAAGGAATAAACAAAAAGCAGTGAATAAGTTGAAAGAGATTGTCGCTAAATTTAACGCCATGATTTTTACCCCaa                                      >  2:130551/1‑144 (MQ=255)                                                                                                                     tAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAATACAAAAAATGGGGTTGCCAT‑GATGAGCGAAAGAAATAAGCAAAAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                              >  2:88249/1‑149 (MQ=255)                                                                                                                         gttAGTTTGTGGGGGTG‑AATTTTTCCAAACAAATACAAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                         <  1:774007/148‑1 (MQ=255)                                                                                                                                 gtgGCGGTG‑AATTTTTTCAGACAAATAAAAAAACTGGAGTTGGCCT‑GATGAGCGGAAGGAATAAACAACAAGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCCAAAGCCTGAAGAAgtggtg                     >  1:803654/1‑146 (MQ=255)                                                                                                                                  tgGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                >  2:922728/1‑150 (MQ=255)                                                                                                                                  tgGCGGTG‑AATTTTCCCATAAAAATACAAAAACTGGAGTTGCCGT‑AGTGGGCGAAAGTAAGAAGCAACAGCCGGTTAATAAGTTGAAAGAGATTGTCCTTAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                <  2:265226/150‑1 (MQ=255)                                                                                                                                   ggCGGTG‑AATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa               <  2:402771/150‑1 (MQ=255)                                                                                                                                   ggCGGTA‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc              <  2:1318448/151‑1 (MQ=255)                                                                                                                                   ggCGGTA‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATGGTGGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                <  1:688778/149‑1 (MQ=255)                                                                                                                                    gCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAGCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa               <  1:1394122/149‑1 (MQ=255)                                                                                                                                    gCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt             >  2:808531/1‑151 (MQ=255)                                                                                                                                       gTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa           >  2:1116435/1‑150 (MQ=255)                                                                                                                                           aaTTTTTCCAGACAAATAAAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa       <  2:1152252/151‑1 (MQ=255)                                                                                                                                                 tCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  2:1398801/1‑150 (MQ=255)                                                                                                                                                  ccAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  2:345286/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                      ATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206291‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑