| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 47.1% | 31.9 / 16.3 | 34 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (10/8); new base A (0/16); total (10/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAAT > NC_000913/357742‑358036 | gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca > 2:139820/1‑150 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc < 1:1003012/151‑1 (MQ=255) cGTAGCGCATCATGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgc < 2:456309/151‑1 (MQ=255) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgcc < 1:54447/151‑1 (MQ=255) gTAGCGCATCAGCAAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgcc < 1:315829/151‑1 (MQ=255) gcgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGtt > 1:156614/1‑151 (MQ=255) tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc > 1:905973/1‑151 (MQ=255) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gg > 1:747356/1‑151 (MQ=255) cGCAGCCCTGTTTCGGGGGCTCCCGTATTCCATTCCCGCAACCACCCGCGCATTTACCCAACGCATTGTGAAAAAATG‑CTTTCGCGGCGGTTTTCGCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑ggg < 1:1019359/149‑1 (MQ=255) cGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gg > 2:396083/1‑148 (MQ=255) acgtgtgTCAGCGGCCCCCGTTATTATTTTCCGCCAAAACCCGCTCTTTTACCCACCACATTTTGAAAAAAGC‑CCTTCGGGGCGTTTGTCTGCAACCTATTTTCCCGCCAAAAAAAAGCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAaga < 1:1047603/147‑1 (MQ=255) tgtCGGCGCCTCCCGTGTTTCTTTCCCCCCAACACCCGCGCTTTTACCCACCGCCATGTAAAAAAATG‑CCTCCGGGGCGGCTGTCTGCCAGTTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGaaa < 1:517893/149‑1 (MQ=255) aGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGGGaaaaa > 1:100914/1‑145 (MQ=255) gCGGCTACCGTGATTCTTTCCCGCAAACACCCGCGCTTTTACCCAACGCCATGTAAAAAAATG‑CCTTCGCGGCGGCTGTCGGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGc < 1:179668/148‑1 (MQ=255) ggCTCCCGTAATTTATTCCCCCCAACACCCCCGCTTTTACCCACCCCCATTTAAAAAATGG‑CCTCCGGGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAAt < 1:1059421/143‑1 (MQ=255) ggCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCGAGACaaaaaaaacaac > 2:299411/1‑142 (MQ=255) ccGTGTTTCTTTCCCGCAAACAACCGGGCTTTTCCCCACCGCTATGGCAAAAAATC‑CTTTCGCGGCGGTTGTCTGCAAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGaaa < 1:494083/137‑1 (MQ=255) ccccccaccaCCCGCGCATTTACCAAACGCCATGTGAAAAAATC‑CCTTCGGGGCGGCTGTCTGCCAGTTATTTTCCCCCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTtgtgt < 1:281620/146‑1 (MQ=255) cccccaccaCCCGCGCATTTACCCACCTCTTTGAG‑AAAAATGCCCTCCGCGGGGGCTGTCTGCCTGTTTTTTCCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgttgt < 2:699225/144‑1 (MQ=255) ccGCAAACAACCGCGCTTTAACCCACCGCCTTGTCAAAAAAGG‑CCTCCGGGGCGGTTGTCCGCCAGCTATTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTgg < 1:122577/151‑1 (MQ=255) ccGCCCTTTTACCCACCGCCATTTAAAAAACTC‑CTTTCGCGGCGGCTGTCTCCCAGCTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 1:722233/148‑1 (MQ=255) cgcATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCGGGGGAAAAAATTTTAA‑CCCGTCATGTTGTGTTGGAATCgccg > 2:887582/1‑145 (MQ=25) ccacccccgtgtgAAAAAATC‑CCTCCGCGGCGGCTGTCTCCCGGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtg < 1:881357/141‑1 (MQ=255) cAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCGAGAGAAAAAAATTTTAACCCCTCACCTTTTTTGGGGATTGCTGCTggggaaaa > 1:598371/1‑141 (MQ=25) acccccATGAGAAAAATTC‑CCTCCGCGGGGGCGTTCGGCATGTTGTTTTCCCGCCGCAAAAAAGCCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTgg < 1:270284/147‑1 (MQ=255) accctgtgttaaaaaaTG‑CCTTCGCGGGGTCTGTCCGCGAGCTTTTGTCCCCCCACAACAAAGCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtgtg < 2:874346/138‑1 (MQ=255) ccccATGTAAAAAAACC‑CTTCCGGGGCGCTTGTCGGCCGCCTTTTTTCCCGCGGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaa < 2:788288/149‑1 (MQ=255) gccgtggccAAAAATC‑CCTGCGCGCCGGCTGCCTCCCATTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaa < 1:944478/142‑1 (MQ=255) aaaaaaGC‑CCTCCGCGGCGGCTTTCGCCGAGCTTTTTTCCCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGt < 2:51127/149‑1 (MQ=255) g‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAGAAAAAAATTTTAACCCGCCCTGTTGTGTGGGGATACCTGCTGGGGAAAATGTGGGAAGGGGAgggggggg > 1:433456/1‑142 (MQ=255) cTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGAAAAAAAAATTTTAACCCCTCCTGGTTTGTGGGGAACGCTGGTGGGAAAAAAGTGGGAAGGGGAgggggggg > 1:988512/1‑140 (MQ=255) ggcggcTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa < 1:798607/146‑1 (MQ=255) tttttgccccgccgAAAAAACTCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAtt < 2:747063/142‑1 (MQ=255) ttttcCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg < 1:618362/148‑1 (MQ=255) ttccccgccgAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGt < 2:937818/146‑1 (MQ=255) aacaaAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAAt < 2:1022656/149‑1 (MQ=255) | GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATG‑CCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAAT > NC_000913/357742‑358036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |