Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,538,109	0	A	C	42.0%	74.1 / 14.9	51	G219G (GGT→GGG)	nanM	N‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base A (26/3);  new base C (0/21);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 3.01e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTT  >  NC_000913/4537962‑4538257                                                                                                                                                     |                                                                                                                                                      ccccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCGCCt                                                                                                                                                    <  2:135960/151‑1 (MQ=255)      cAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑Att                                                                                                                                              >  2:215515/1‑151 (MQ=255)        aaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATACTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTc                                                                                                                                             >  1:145961/1‑150 (MQ=255)               cAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTTGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAAccgc                                                                                                                                     <  1:569318/151‑1 (MQ=255)               cAGCTACGCCATCTGGTGATGACACGGGAGCAAGCTTTTTCGATTTTAAATTATTAAGGGTGAAATCAAGTTCAAATACGGCAGCCGTTCGAAATACTGGTATGGCTT‑CACCATTAATAAGCAAGGTTTTATGACCTTT‑ATTTACAAc                                                                                                                                        >  1:288063/1‑148 (MQ=255)                         aTCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGcc                                                                                                                           >  2:258457/1‑151 (MQ=255)                             ggTGAGGCGGCGGGGGCAAGTTTTTCCCATTTTAATTTATACCCGGTGAAACAAAGTTCAAATACCGCTCCCGTCCCCAGTCGTGTTTTGTCCC‑CCCCCTTAATGCGGCATGTTTTACCCCGTCT‑TTTCACAACCGCCGCACCAGcc                                                                                                                           <  1:37293/147‑1 (MQ=255)                                gatgaGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGt                                                                                                                    >  2:23275/1‑151 (MQ=255)                                   gagaCGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTAcc                                                                                                                 >  1:342736/1‑151 (MQ=255)                                        gggAGCAAGTTTATTCCTTTTAAAATTATTCCCGGTGAAATCAAGTTCAAATACGGCACCCGTTCGCAACCCTGGTTTGGCTT‑CCCCATTAATAAGCCAGTTTTTACCCCCTTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAg                                                                                                               <  2:610266/148‑1 (MQ=255)                                        gggAGCAAGCTTATTCCATTTTAAATTATTCCCGTTGAAATCAAGTTCAAATACGGCACCCGTTCGCAATCCTGTTTTGGCTT‑CCCCATTAATAAGCCAGGTTTTATCCCCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGc                                                                                                            <  2:377739/151‑1 (MQ=255)                                        gggAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAggg                                                                                                             >  1:287222/1‑150 (MQ=255)                                             cAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTc                                                                                                       >  2:77545/1‑151 (MQ=255)                                               aGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                     >  2:5600/1‑151 (MQ=255)                                                       ccATTTTAAATTATTACCGGTGAAATCACGTTCAAATACGGCATCCGTTCGCAATACTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACTTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCTGGGCGATTCGCCAGCGTa                                                                                              >  1:375338/1‑150 (MQ=255)                                                        cATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAAc                                                                                            >  2:369934/1‑151 (MQ=255)                                                         aTTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACt                                                                                           >  2:486772/1‑151 (MQ=255)                                                          ttttAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTc                                                                                          >  2:47062/1‑151 (MQ=255)                                                           tttATATTCTCACCGGTGAAATCAAGTTCCAATAAGGCGTCCGTTTGCAATCCTGGTCTGTCTC‑CGCTCTTACTCATCCAGGTTTTATCACCTTT‑ATTCACAATCGCCGCTCCAGCCGTGCCGTAGCAGGGTGATTCGCGAGCGTAACtac                                                                                         >  1:293799/1‑149 (MQ=255)                                                            ttAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc                                                                                         >  1:409057/1‑150 (MQ=255)                                                             tAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAt                                                                                       >  1:79258/1‑151 (MQ=255)                                                                    ttccgggTAAAATCAATTTAAAATCCGGCTCCCGTTCGAAATCCTGGTTTGGTTT‑CGCCATAAAAAAGCCAGTTTTTATCCCCTTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt                                                                                 <  2:532290/145‑1 (MQ=255)                                                                     taCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt                                                                               >  2:42474/1‑151 (MQ=255)                                                                       ccGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTATCTCCATTGCTGTGTTg                                                                             >  2:197073/1‑151 (MQ=255)                                                                       ccGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                              >  1:215271/1‑150 (MQ=255)                                                                                  aaGTTCAAATCCGGCTCCCGTTCGCATTCCTGTTTTGGCTT‑CGCCATAAATAAGCCAGTTTTTACCCCCTTT‑ATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa                                                                    <  2:189921/149‑1 (MQ=255)                                                                                   atttaaaaTACGCCACCCTTTCCCAACCCTGTTTTGGTTT‑CCCCATTAAAAAGCCAGTTTTTACCCCTTTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAg                                                                 <  1:342678/146‑1 (MQ=255)                                                                                        aaatcgggCCTCCTTTCGCATCCTTGTTTTGGTTT‑CGCAATTAAAAAGCCGGTTTTTACCCCCTTT‑ATCCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGacaac                                                            <  2:7089/145‑1 (MQ=255)                                                                                              ggCATCCTTTCACAATCCTGGTTTGGCTT‑CCCCATTAATAAGCCAGTTTTTATCCCCTTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaa                                                        <  2:157413/149‑1 (MQ=255)                                                                                                 aTCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                    >  1:585041/1‑150 (MQ=255)                                                                                                 aTCAGTTCGCAATCCTGGATTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACAAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                    >  1:449593/1‑150 (MQ=255)                                                                                                   ctgTTCGCAACCTTGGTTGGGCTC‑CGCCATAAAAAACCCATTTTTTCCCCCCTTA‑ATACACCCCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                   <  1:539844/147‑1 (MQ=255)                                                                                                           aaTCCTGTTTTGGCTT‑CCCCATTAAAAGGCCAGGTTTTATCCCCTTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                   <  2:563192/141‑1 (MQ=255)                                                                                                           aaTCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaat                                         >  2:395851/1‑151 (MQ=255)                                                                                                                tgttttGGTTT‑CGCCTTTAATAACCCAGGTTTTATCCCCTTT‑ATACACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaataat                                      <  2:243996/146‑1 (MQ=255)                                                                                                                  ggttGGCTTCCGCCA‑TAAAAAGGCTGGTTTTTCCCCCTTTAATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaaga                                            <  1:110826/140‑1 (MQ=255)                                                                                                                         tt‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                           >  1:73207/1‑151 (MQ=255)                                                                                                                            cGCCATTAATAAGCCAGTTTTTATCCCCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttttt                         <  2:653696/151‑1 (MQ=255)                                                                                                                             gCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTg                        >  2:600083/1‑151 (MQ=255)                                                                                                                                 ttaaaacgccgttttttATCCCTTTT‑TTTCCCAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCt                              <  1:77520/128‑1 (MQ=255)                                                                                                                                 ttAATAAGCCAGGTTTTATCCCCTTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTg                        <  2:263089/147‑1 (MQ=255)                                                                                                                                      acgCCGGGTTTTACCCCTTTT‑TTCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAg               <  1:109130/149‑1 (MQ=255)                                                                                                                                      acccctgtttttAACCCCTTT‑TTACACAGCCGCCTCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaaa                  <  1:79371/140‑1 (MQ=255)                                                                                                                                       accaattttttcacACCTTT‑ATCAAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATa                <  1:448489/136‑1 (MQ=255)                                                                                                                                         ccAGTTTTTCCCCCCTTT‑TTTACCAACCGCCGCCCCAGCCGTTCCGTACCAGGGAGATTCGCCAGCGTAACTCCATTGCTGTATTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATa                <  2:275660/147‑1 (MQ=255)                                                                                                                                         ccAGTTTTTATCCCCTTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAg               <  2:231300/148‑1 (MQ=255)                                                                                                                                          catgTTTTACCCCCTTT‑ATCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGa            <  2:239492/147‑1 (MQ=255)                                                                                                                                            gtttttACCCCCTTT‑ATCCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGc          <  2:575457/148‑1 (MQ=255)                                                                                                                                              ttttATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAtt       >  2:457064/1‑151 (MQ=255)                                                                                                                                               tttATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATACTCTTCTGCTTTTTTGTCAAAATAGTGAGCAtt       >  1:573035/1‑150 (MQ=255)                                                                                                                                                    cACTTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAttt  <  1:544162/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTT‑CGCCATTAATAAGCCAGGTTTTATCACCTTT‑ATTCACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTT  >  NC_000913/4537962‑4538257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑