Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,662	0	T	A	42.2%	96.2 / 22.6	64	E384D (GAA→GAT)	yhfW	phosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (4/33);  new base A (27/0);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 3.72e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCA  >  NC_000913/3508512‑3508807                                                                                                                                                         |                                                                                                                                                  ccACGTTGTTGTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTGTGTGGCGGTGG‑CGCG‑CGGAAAAAt                                                                                                                                                   <  1:117636/151‑1 (MQ=255)       tgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATcaca                                                                                                                                              >  2:839168/1‑150 (MQ=255)       tgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTcaca                                                                                                                                              <  1:503301/150‑1 (MQ=255)             tAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGt                                                                                                                                        <  1:501433/150‑1 (MQ=255)              aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTa                                                                                                                                       <  2:932362/150‑1 (MQ=255)                    ggAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑GGCG‑CGGGAAAAATTACAAACGGGAGccccc                                                                                                                                 >  2:136438/1‑150 (MQ=255)                     gagagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATTAAACACGGGAGccccccc                                                                                                                               >  2:619697/1‑149 (MQ=255)                        agaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATc                                                                                                                            <  1:246022/151‑1 (MQ=255)                             aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATTACACACGGTAGCCCCCACCTCAGaa                                                                                                                        >  1:547315/1‑150 (MQ=255)                             aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGaaa                                                                                                                       <  1:924603/151‑1 (MQ=255)                             aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGaaa                                                                                                                       <  1:569498/151‑1 (MQ=255)                              atatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAg                                                                                                                      <  2:465474/151‑1 (MQ=255)                                     gATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtggtg                                                                                                               <  1:279008/151‑1 (MQ=255)                                      aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑GGCG‑CGGGAAAAATCAAACAACGGAGCCCCCACAACAGAAAGCgggggg                                                                                                               >  2:48653/1‑148 (MQ=255)                                           cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGcac                                                                                                          <  1:793865/150‑1 (MQ=255)                                                  gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCt                                                                                                  <  1:890795/151‑1 (MQ=255)                                                   aGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATCACACACCGTAGCCCCCACATCAGAAAGCGGGGGGCGCACACCGa                                                                                                     >  2:409026/1‑147 (MQ=255)                                                     ggTGTAACCCGTCGGATCACATCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAGGAGCGACCATCTTGTGGCGGTGG‑CTCG‑CGGAAAAATACACACGCGGTAGCCGCCGCATCAGCACGCGTGGTGCGCTCACAGAGCTGCg                                                                                               <  1:244299/151‑1 (MQ=255)                                                        gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTa                                                                                             <  2:802969/150‑1 (MQ=255)                                                         tAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑GGCG‑CGGGAAAAATTACACACGGTAGCCCCCCCATCAGAAAGAGTGGGGGGCACACCCAGCTGCGGAg                                                                                            >  2:301845/1‑150 (MQ=255)                                                          aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCg                                                                                          <  1:723957/151‑1 (MQ=255)                                                             ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATTACACACAGTAGCCCCCCCCTCAGAAAGGGGGGGGGGCCCACCGAGCCGCGTAGCggt                                                                                        >  1:197454/1‑148 (MQ=255)                                                               gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAAATCACACACGGTAGCCCCCCCATCAGAAAGCGTGGGGCGCACACCCAGCTGCGGAGCGatca                                                                                      >  2:518430/1‑150 (MQ=255)                                                                  ggATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATccc                                                                                  <  2:700423/151‑1 (MQ=255)                                                                      cAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTg                                                                               <  1:1011428/150‑1 (MQ=255)                                                                       aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGGG‑CGGGAAAAATTACACACGGGAACCCCCCCATCAGAAAGGGGGGGGGGCACACCGAGGTGCGGAGGGATTATCCCtt                                                                                >  1:916076/1‑148 (MQ=255)                                                                        aaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGGG‑CGGGAAAAATCACACACGGGAGCCCCCACATCCGAAAGCGGGGGGGGCGCACCGAGCTGCGGAGCGAGCATCCCtttc                                                                              >  1:490414/1‑147 (MQ=255)                                                                         aaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGa                                                                           <  2:22872/151‑1 (MQ=255)                                                                          aCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGAt                                                                          <  2:722803/151‑1 (MQ=255)                                                                           cTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑GGCG‑CGGGAAAAATTACACACGGTAGCCCCCACATCAGAAAGCGGGGGGCGCACACCGAGCTGCGTAGCGATCATccc                                                                                  >  2:351017/1‑142 (MQ=255)                                                                             cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATaa                                                                        <  2:137992/150‑1 (MQ=255)                                                                             cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATaa                                                                        <  2:887648/150‑1 (MQ=255)                                                                              aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAc                                                                      <  1:377103/151‑1 (MQ=255)                                                                              aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGG‑CGCG‑CGGAAAAAATCACACACGGTAGCCCCCCCCTCAGAAAGCGGGGGGCGCACACCGCGCTGCGTAGCGATCATCCCTTTCTGAaaaa                                                                       >  1:399582/1‑150 (MQ=255)                                                                                   gtgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATCACACACGGTAGCCCCCACATCAGAAAGCGGGGGGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATaaaac                                                                     >  2:181430/1‑145 (MQ=255)                                                                                    tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑GGCG‑CGGGAAAAATTACACACCGTAGCCCCCCCATCAGAAAACGGGGGGGGCACACCGAGCTGCGCAGCGATCATCCCCTCCTGATAAACCAGCAc                                                                >  2:164866/1‑151 (MQ=255)                                                                                       ctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATCACACACGGTAGCCCCCCCATCAGAAAACGTGGGGGGCACACCGAGCTGCGGAGCGATCATCCCCTGCTGATAAAACAGCACTgg                                                             >  2:63247/1‑151 (MQ=255)                                                                                         ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGca                                                           <  2:139766/151‑1 (MQ=255)                                                                                            gCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcacc                                                         <  2:450551/150‑1 (MQ=255)                                                                                             cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATTACACACGGTAGCCCCCCCATCAGAAAACGGGGGGGGCACACCCAGCTGCGTAGCGATCATCCCCTTCTGGTAAAACAGCACTGGcacc                                                         >  2:641519/1‑149 (MQ=255)                                                                                              aaaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATTACACACGGTAGCCCCCCCATCAGAAAACGGGGGGCGCACACCCAGCTGCGTAGCGATCATCCCTTGCTGATAAAACACCACCGGcaccac                                                       >  2:602290/1‑150 (MQ=255)                                                                                               aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATTAAAAACGGGAGCCCCCCAAACAAAAAACGTGGGGGGGACACCCAACGGCGTAGCGAACATCCCCTTCCGGTAAAACACCACCGGcccccc                                                       >  1:951160/1‑147 (MQ=255)                                                                                               aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATCACACACGGGAGCCCCCCCCTCCGAAAACGGGGGGCGCACCCCCAGCTGCGGCGCGATCATCCCCTGCTGATAAAACAGCCCTGGcaccac                                                       >  1:906475/1‑149 (MQ=255)                                                                                               aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATCACACACCGTAGCCCCCCCATCAGAAAAAGGGGTGCGCACACCCAGCTGCGTAGCGATCATCCCCTGCTGATAAAACAGCACCGGcacc                                                         >  2:188547/1‑147 (MQ=255)                                                                                                aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑GGCG‑CGG‑GAAATTCACACACGGGAGCCCCCCCAACAGAAAGCGTGGGGCGCCCACCCAGCTGCGTAGCGATCAACCCTTGCTGATAAACCAACACTGGcaccac                                                       >  2:744232/1‑147 (MQ=255)                                                                                                aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATCACACACGGTAGCCCCCCCATCAGAAAACGTGGTGGGCACACCCAGCCGCGGAGCGATCATCCCTTGCTGATAAACCAGCACTGGcaccacct                                                     >  2:854290/1‑148 (MQ=255)                                                                                                   ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCTCTGGCACCACTTcgcg                                                 <  1:283196/151‑1 (MQ=255)                                                                                                   ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgcg                                                 <  2:339873/151‑1 (MQ=255)                                                                                                         aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATTACAAACCGTAGCCCCCACCACAGAAAACGGGGGGCGCACACCCAGCTGCGTAGCGAACCACCCTTGCTGATAAAACAGCACCGGCACCCCCTCTCGGGGAtg                                           >  2:814397/1‑151 (MQ=255)                                                                                                         aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtg                                           <  1:824293/151‑1 (MQ=255)                                                                                                           gaaagaaaAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCAGATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTGGCGATCATCCCTTGCTGATGAACCAGCACTGGCACCGCTTCGCGGGTAtggt                                         <  1:706636/151‑1 (MQ=255)                                                                                                            aaagaaaagaGCGACCATTTTGTGGCGGTGG‑GGCG‑CGGGAAAAATCACACACGGTAGCCCCCACCTCAGAAAGCGTGGGGCGCACACCCAGCCGCGTAACGATCATCCCTTTCTGATAAAACAGCACTGGCACCACTTCGCGGGGAtgggg                                        >  2:82821/1‑149 (MQ=255)                                                                                                             aagaaaagaGCGACCATTTTGTGGCGGTGG‑GGCG‑CGGGAAAAATCACAAAAGGGAGCCCCCACATCAGAAAGCGGGGGGGGCCCACCCAGCTGCGTAGCGATCATCCCCTGCTGATAAAACAGCACCGGCACCACCTCGCGGGGAtggggg                                       >  1:450551/1‑151 (MQ=255)                                                                                                                aaaagaGCGACCATTTTGTGGCGGTGG‑CGGGCCGGAAAAATT‑AAAAACGGGAGCCCCCCCAACAGAAAACGGGGGGGGGACACCCAGCCGGGCAACGAACCACCCCTTCCGATAAAACAACACCGGCACCACCTCCCgggggtggggggg                                     >  1:644457/1‑140 (MQ=255)                                                                                                                   agagCGACCATTTTGTGGCGGTGGCCCCC‑CGGAAAAATT‑ACACACCGGAACCCCCCCAACAGAAAACGGGGGGGGCCCCCCCCGCTGCGGAGCGGTCCTCCCTTTCCGAAAAAACCGAACCGGCACCCCTTCCCGGGGGTGGGGGGtgtg                                  >  1:49626/1‑150 (MQ=255)                                                                                                                        gACCATTTTGTGGCGGTGG‑CGCG‑CGGGAAAAATCACACACGGGAGCCCCCCCATCAGAAAACGGGGGGCGCACACCCAGCTGCGTAGCGATCATCCCTTTCTGTTAAAACAGCACTGGCACCACTTCGCGGGGATGGGGGGTGTGACCaa                             >  2:244395/1‑150 (MQ=255)                                                                                                                        gACCATTTTGTGGCGATGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACAACTTCGCGGGTATGGTGGCTGTGACCaa                             <  1:101877/150‑1 (MQ=255)                                                                                                                           cATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGt                         <  2:286272/151‑1 (MQ=255)                                                                                                                              tttGTGGCGGTGG‑CGCG‑CGGGAAAAATCACACACCGGAGCCCCCCCATCAGAAAGCGTGGGGGGCACACCCAGCCGCGGAGCGATCATCCCCTGCTGATAAAACAGCACTGGCACCACTTCGCGGGGATGGGGGGTGTGACCAATGGGCgg                      >  2:617932/1‑151 (MQ=255)                                                                                                                                   ggcggtgg‑cgCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg                  <  2:1002315/150‑1 (MQ=255)                                                                                                                                   ggcggtgg‑cgCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg                  <  2:450935/150‑1 (MQ=255)                                                                                                                                       gtgg‑cgCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGcc             <  1:575016/151‑1 (MQ=255)                                                                                                                                                 cgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg     <  1:854096/151‑1 (MQ=255)                                                                                                                                                  ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGc    <  1:518332/151‑1 (MQ=255)                                                                                                                                                    aaaaaTTCACACACGGTCGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCCGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGCCGGATCGTTGCCGTGATCCGccc  >  1:165179/1‑150 (MQ=255)                                                                                                                                                         |                                                                                                                                                  CCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGG‑CGCG‑CGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCA  >  NC_000913/3508512‑3508807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑