Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,605	0	T	C	47.3%	95.7 / 24.5	75	G187G (GGA→GGG)	ycfT	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (30/9);  new base C (0/35);  total (30/45)
Fisher's exact test for biased strand distribution p-value = 5.87e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGT  >  NC_000913/1174458‑1174751                                                                                                                                                       |                                                                                                                                                     caacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTccc                                                                                                                                                   >  1:453675/1‑151 (MQ=255)  aacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTcccc                                                                                                                                                  >  1:130501/1‑151 (MQ=255)      aGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCccacc                                                                                                                                               >  1:358264/1‑150 (MQ=255)      aGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCccacc                                                                                                                                               >  1:628426/1‑150 (MQ=255)       ggCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCcaccac                                                                                                                                             >  1:1063670/1‑151 (MQ=255)        gCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACg                                                                                                                                            <  2:822793/151‑1 (MQ=255)        gCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACg                                                                                                                                            <  1:347753/151‑1 (MQ=255)         cACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTGCCCACCACgg                                                                                                                                           <  1:1013509/151‑1 (MQ=255)          accgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGc                                                                                                                                          <  2:719953/151‑1 (MQ=255)          accgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGc                                                                                                                                          >  1:664103/1‑151 (MQ=255)          accgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGc                                                                                                                                          >  1:645215/1‑151 (MQ=255)            cgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGt                                                                                                                                        <  1:950293/151‑1 (MQ=255)              accgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTgg                                                                                                                                      <  1:881621/151‑1 (MQ=255)                   cAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGTGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAAc                                                                                                                                  >  2:863337/1‑150 (MQ=255)                     gAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGaa                                                                                                                               <  2:851539/151‑1 (MQ=255)                     gAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGa                                                                                                                                >  2:612394/1‑150 (MQ=255)                      aaCGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGaa                                                                                                                               >  2:874165/1‑150 (MQ=255)                       aCGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCACACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑t                                                                                                                            <  2:787140/151‑1 (MQ=255)                             aGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCg                                                                                                                     <  2:125094/151‑1 (MQ=255)                              gCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGc                                                                                                                    >  2:9159/1‑151 (MQ=255)                                aaaGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGcc                                                                                                                   >  1:1117284/1‑150 (MQ=255)                                       ccaccaGCAGATGGCGGCGCAACGGCCCCTCTTAAACACAGGTCATTATTGTTGCGCCAAACAAT‑GCGCAAAGGCTGTAAAAAGGCAAATG‑GC‑GGATCACACTGTTCTTCCCCCCCCACGGCGTGGAAACGAAA‑TTAA‑CCGCCACACTca                                                                                                            <  1:190797/150‑1 (MQ=255)                                         aTCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGCCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTcagc                                                                                                          >  2:111812/1‑150 (MQ=255)                                            agcggATGGCGGCGCACCGGCCCCTCTTAAACACAGATCTTTATTTTTCCGCAAAACCAT‑GCGCAAAGGCTGTAAAAAGGAAATTT‑GG‑GGATCCCCCTGTTCATCCCCCCCCGCGGGGGGGAAACAAAA‑TAAA‑CCGCCACACTCAGCAGTa                                                                                                      <  1:546903/151‑1 (MQ=255)                                             gcagATGGCGGCGCAACGGCCCCTCTTTAACACAGGTTATTATTGTTGCGCAAAACCAG‑GCGCCAAGGTTGTAAAAAGCCAATTG‑GC‑GGATCACACTTTTCATCCCCCCCCACGGCGTGGAAACAAAA‑TTAA‑CCGCCACACTCAGCAGTa                                                                                                      <  2:743101/150‑1 (MQ=255)                                                   ggcggcGAAACGCCACCTTTTTAACACAGGTAATTATTGTGGCGCCAAACCAT‑GCGCAAAGGCTGAAAAAAGGCAAATG‑GC‑GGATCACATTGTTCATCCCCCCCCACGGCGTGGAAACAAAA‑TTAA‑CCGCCACACTCAGCAGTa                                                                                                      <  2:1057091/144‑1 (MQ=255)                                                         gcAAGGGCACTTTTTTAACAGAGGTTATTATTGTGGC‑CCAAACCCTGGCGCCACGTATGTAA‑AAGGGAAATTGGG‑GGATCCCATTGTTTATCCCCCCCCGCGGCG‑GGGAAGTAAATTTAC‑CCGCCACACTCAGCAGTACAAACAAGGCg                                                                                           <  2:110933/149‑1 (MQ=255)                                                           aaCGCCCCCTTTTAAACACAGGTTATTATTTTTCCCCCAACCCAT‑GCCCAAGGGGTGTAAAAAGAAAAATT‑GG‑GGATCCCCCTTTTCATCCCCCCCCACGGGGGGGAAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATa                                                                                       <  1:253175/151‑1 (MQ=255)                                                            aCGGCACCTCTTAAACACAGGTCATTTTTGTTGCGCCAACCAGT‑GCGCCAAGGCTAAAAGAAGGAAAAGT‑GC‑GGATCACCCTGTTATTCCCCCCCCACGGCG‑GGGAAAGAAA‑ATAAGCCGCCACACTCAGCAGTACAAACAAGGCGAATa                                                                                       <  1:838878/150‑1 (MQ=255)                                                             cGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGt                                                                                     >  2:807476/1‑151 (MQ=255)                                                                cACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGc                                                                                  >  2:627421/1‑151 (MQ=255)                                                                cACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGc                                                                                  >  1:1099891/1‑151 (MQ=255)                                                                  cctcttaaaacaaGGTTATTATTGTGGCGCAAAACAAG‑GCGCCAGGGTTGAAAAAAGGAAAATG‑GC‑GAACCACTCTGTTCATCCCCCCCCACGGGGGGGGAAAAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCa                                                                                 <  1:197845/138‑1 (MQ=255)                                                                  ccTTTTAAACACGGGTCATTATTGTTGCGCAAACCCAG‑GCGCCAGGGCTTAAAAAAGGAAAATT‑GG‑GAACCACCCTTTTCTTCCCCCCCCACGGCGGGGAAAAGAAA‑TAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCa                                                                                 <  1:1181736/150‑1 (MQ=255)                                                                  ccTCTTAAACCAAGTTAATTATTGTGGCGCCAACCCAG‑GCGCAAAGGCTGTAATAAGGAAAATG‑GC‑GGATCACATTGTTCATCCCCCCCCACGGCGTGGGAAAAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGt                                                                                     <  2:106968/146‑1 (MQ=255)                                                                         aaCACGGTTCTTTTTTTTTCCGCAAAACCTG‑GCGCCAGGGTTATAATAAGGAAATTT‑GC‑TGATCACATTTTTCATCCCCCCCCCGGGGGTGGGAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCa                                                                           <  2:711308/149‑1 (MQ=255)                                                                         aaCACGGCTCATTATGTTTGCGAAAACCCCG‑GCGCCAAGGCTGTAA‑AAGGGAAATT‑ACAGGATCCTGCTGTCGTTACCCCACCAGGGGGTGGAGACAAAA‑TTAG‑CCGCCACCCTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGACAGa                                                                         <  1:334975/151‑1 (MQ=255)                                                                         aaCACAGGTCATTTTTGTTGCGCAAAACCAT‑GCGCCAAGGCTGAAAAAAGGAAAATT‑GC‑GGATCACCCTTTTCATCCCCCCCCCCGGCGGGGAAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGa                                                                         <  2:858452/151‑1 (MQ=255)                                                                         aaCAAAGTTCATTTTTTTGGCGCAAACCAAG‑GCCCCAGGGTTTAAAAAAGGAAAATT‑GC‑GAATCACTCTTTTCATCCCCCCCCCCGGGGTGGAAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAg                                                                          <  2:710733/150‑1 (MQ=255)                                                                          acacAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGAGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGa                                                                         >  1:548011/1‑150 (MQ=255)                                                                                   attattGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaaa                                                                >  1:453756/1‑150 (MQ=255)                                                                                    tttttgtggCCCAAAACCAG‑GCGCCAAGGCTGTAATAAGAAAAATT‑GG‑GGATCCCCCTTTTCATCCCCCACCACGGCGGGGGAACAAAA‑TTAC‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCt                                                                    <  1:915627/142‑1 (MQ=255)                                                                                       tttttgCGCAAACCCTT‑GCGCAAGGGCTTAAATAAGGCAATTG‑GC‑GATCCCCATTGTTCTTCCCCCCCCAGGGCGGGGGAACAAAT‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAAt                                                              <  2:390192/145‑1 (MQ=255)                                                                                       ttgttgCCCAAAACCAT‑GCCCCAGGGCTTAAAAAAGGAAATTT‑GG‑GGATCACCCTTTTCATCCCCCCCCCCGGCGAGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAAtt                                                             <  2:379405/149‑1 (MQ=255)                                                                                          tgccgacAACCCAT‑CCCCCAGGGCTTAAATAAGAAAAATG‑GC‑GCATCACACTGTTCATCCCCCCCCCCGGGGGGAAAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaa                                                                 <  1:176822/136‑1 (MQ=255)                                                                                              gcCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATAcc                                                    >  2:448327/1‑151 (MQ=255)                                                                                               ccAACCCAT‑GCGCCAGGGCTGTAATAGGGCAAATT‑GC‑GGATCACACTTTTCTTCCCCCCCCACGGGGTGGAAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCa                                                   <  2:586179/151‑1 (MQ=255)                                                                                                 aaaCCAT‑GCCCCAGGGTTGAAAAAAGGCAAATG‑GC‑GGACCACCCTGTTCCTCCCCCCCCCCGGCGGGGGAACAAAA‑TAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCAc                                                  <  1:158387/150‑1 (MQ=255)                                                                                                 aaaCCAT‑GCCCCAAGGATCAAATAGGAAAATTT‑GC‑GTATCCCTCTGTTCATCCCCCCCCGCGGCGTGGGAAAGAAA‑GTAT‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACg                                                 <  2:408458/151‑1 (MQ=255)                                                                                                   aCCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGa                                                >  2:408346/1‑150 (MQ=255)                                                                                                    ccAG‑GCCCCAGGGCTTAAATAGGGAAATTT‑GC‑GGACCCCACTTTTCATCCCCCACCAGGGCGGGGAAACAAAA‑TAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCa                                                   <  1:878515/146‑1 (MQ=255)                                                                                                     cAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGaaa                                              >  1:1188765/1‑150 (MQ=255)                                                                                                       aggcgcAAGGGCTGTAAAAAGGAAAATG‑GC‑GGATCCCACTGTTCTCCCCCCACCACGGGGTGGGAAAAAAA‑TAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAAt                                             <  1:136901/148‑1 (MQ=255)                                                                                                            caaaGGCTGAAATAAGGCAAATG‑GC‑GGATCACACTGTTCATCCCCCACCCGGGGGGGGGAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACa                                        <  2:418701/148‑1 (MQ=255)                                                                                                             cAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAAt                                      >  1:663800/1‑151 (MQ=255)                                                                                                              aaGGCTGAAAAAAGCAAAATT‑GC‑GAACCACACTTTTCATCCCCCCCCACGGCGTGGAAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGATGGCTAAAAATTTTACATACCACGAAATAGACaa                                       <  2:1063722/149‑1 (MQ=255)                                                                                                                ggCTGTACTAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCGGTACAAACAAGGCGAACAGTGGCAGGGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTa                                    >  1:1089325/1‑150 (MQ=255)                                                                                                                gcttgaaaaagggaaaaTG‑GC‑GGATCCCCCTGTTCTTCCCCCCCCCCGGGGGGGGAAGAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTaa                                   <  2:166352/137‑1 (MQ=255)                                                                                                                      aataaGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCAt                              >  2:502390/1‑150 (MQ=255)                                                                                                                      aataaGGAAAATT‑GC‑GGATCACACTTTTCTTCCCCCCCCACGGCGGGGAAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCa                               <  2:845147/149‑1 (MQ=255)                                                                                                                         aaGGAAAATT‑GC‑GGATCACCCTTTTCATCCCCCCCCCGGGCGTGGGAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAg                          <  2:191703/151‑1 (MQ=255)                                                                                                                          aGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATAc                            >  1:138380/1‑148 (MQ=255)                                                                                                                           ggaaaaTT‑GC‑GGATCACACTTTTCATCCCCCACCACGGCGGGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGa                         <  2:1075532/147‑1 (MQ=255)                                                                                                                           ggaaaaTG‑GC‑GGTTCACACTGTCCATCCCCCACCCCGGCGGGGAAACAAAA‑TTAC‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGa                         <  2:3147/147‑1 (MQ=255)                                                                                                                            gCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGAt                        >  2:576092/1‑150 (MQ=255)                                                                                                                            gCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGAt                        >  1:1075129/1‑150 (MQ=255)                                                                                                                              aaaTT‑GC‑GGCTCACCCTGTTCATCCCCCCCCAGGGCGTGGGAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATAc                      <  1:688309/150‑1 (MQ=255)                                                                                                                                     gcgaataccacTTTTCTTCCCCCCCCCGGGGGTGGAAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACaa                 <  2:1193899/142‑1 (MQ=255)                                                                                                                                        gATCACCCTTTTCATCCCCCCCCACGGCGTGGAAAAAAAA‑TAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTg             <  1:425493/151‑1 (MQ=255)                                                                                                                                           cacaCTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTg          >  2:861342/1‑151 (MQ=255)                                                                                                                                                tGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTgg      >  1:608583/1‑150 (MQ=255)                                                                                                                                                  ttCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCgg   >  1:144957/1‑151 (MQ=255)                                                                                                                                                  ttCATCCCCCACCACGGGGGGGAAACAAAA‑TAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCg    <  1:996765/150‑1 (MQ=255)                                                                                                                                                    cATCCCCCCCCACGGGGGGGAAACAAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCg    <  2:152071/148‑1 (MQ=255)                                                                                                                                                    cATCCCCCACCGCGGGGGGGAAACGAAT‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGt  <  2:516496/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                     CAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCAT‑GCGCCAAGGCTGTAATAAGGCAAATT‑GC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAA‑TTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGT  >  NC_000913/1174458‑1174751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑