Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	43.4%	95.9 / 60.2	83	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (39/8);  new base A (0/36);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 4.41e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29347‑29642                                                                                                                                                         |                                                                                                                                                   ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                    <  1:466255/150‑1 (MQ=255)  tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAAtata                                                                                                                                                   <  1:431582/150‑1 (MQ=255)   aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATaaa                                                                                                                                                 <  1:462129/151‑1 (MQ=255)      gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                               >  2:800600/1‑150 (MQ=255)        aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATc                                                                                                                                            <  1:592883/151‑1 (MQ=255)         aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                             <  1:271255/149‑1 (MQ=255)          aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                          >  2:572552/1‑151 (MQ=255)          aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                          <  2:471574/151‑1 (MQ=255)              tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGcc                                                                                                                                       >  2:80816/1‑150 (MQ=255)               gACCATTTGGTCCACTTTTTTCTGCTCTTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGTTT‑ATATGATCTTTTTGTCCCTGAATGCC‑GGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                                      <  1:1092451/150‑1 (MQ=255)                 ccATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                                    >  2:977391/1‑150 (MQ=255)                  cATTTGGGCCACTTTTTTCTGCCCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAAAACAGGTT‑AGATGATCTTTTTTTCGCTTAATGCC‑TGCAAAACTTGCATGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                                      <  1:600388/147‑1 (MQ=255)                   aTTTGGTCCACTTTTTTATGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                   >  2:579875/1‑149 (MQ=255)                      tGGTCCTTTTTTTTGTTTTCTTTTTTATTTCATGCAATCTTCTTGCTGCGCAGGGTTTTTC‑AAAAAAAGGTT‑ATATGATTTTTTTTTCGTTTAATCCC‑TAAAAAACAGGTATGACCCAAAAAAAAATAAAAAAATCCCCGCCATTAAGtt                                                                                                                               <  1:826078/150‑1 (MQ=255)                        gTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGAc                                                                                                                            >  2:505746/1‑151 (MQ=255)                          ccctttttttCTGCTCTTTTTTTTTTCATGCATTTTTCTTGCTGCGCAAGCTTTTTC‑CAAAACAGGTT‑AGATGATTTTTTTGTCGTTAAATCCC‑TGAAAAACATGCAGGACCCAAAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                               <  1:148416/142‑1 (MQ=255)                             tttttttCTCCTCTTTTTTTTTTCAGGAATTTTTCTGGCGGCCAAAGGTTTTCC‑CAAAAAAGGTT‑AGATGATTTTTTTGTCGCTAAAGGCT‑TAAAAAACAGGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                         <  2:383856/148‑1 (MQ=255)                              tttttttcccTCGTTTTTATTTCATGCATTCTTCTTGCGGCG‑AAGGTTTTTCAAAAAACGGGTA‑AGAGTATTTTTTTGACGTTTAATGCG‑TCTAAAACATCCATGACCCAAAAAATAAAAAAAAAATTCCCGCCATTAAGTTGACTTTTa                                                                                                                       <  1:201734/141‑1 (MQ=255)                              ttttttCTGCTCTTTTTTATTTCATGCATTCTTCTTGCGGCGCAAGCGTTTCC‑AAAAACGGTTG‑AGATAATTTTTTTGTCGTTAAATGCC‑TAAAAAACATGCAGGACCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTa                                                                                                                       <  2:822192/150‑1 (MQ=255)                              ttttttCTGCTCTTTTTTATTTCATGCAATCTTCTTGCTGCGCAGGCTTTTTC‑CAAAACAGGTT‑AGATGATTTTTTTGTCGCTTAATGCC‑TGAAAAACATGCATGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                        <  2:645916/149‑1 (MQ=255)                               tttttCTTCTTTTTTTTTTTTCATGCAATCTTCTTGCTGGCCAGCCGTTTAC‑AAAAACAGGTT‑AAATAATCTTTTTGTCACTAATTGCC‑AGTAAAACATGCATCGGCCAAAAAATAATATAAAAACTCCCGCCATTAAGTTGACTTTTAg                                                                                                                      <  1:450310/150‑1 (MQ=255)                                    cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                 >  1:1013627/1‑150 (MQ=255)                                    cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGAGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                 >  1:279985/1‑150 (MQ=255)                                       cttttttttATTTCATGCAATTTTTTTGCTGCGCAGGCGTTTCC‑CAAAAGAGTTT‑AGATGTTTTTTTTGTCGCTTAATCCC‑TATAAACCATGCAGGAGCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCa                                                                                                                <  2:811196/144‑1 (MQ=255)                                        tttttttttttTCATGCATTCTTTTTGCTGCGCAAGCTTTTCC‑AAGAACAGTTT‑AGATGTTTTTTTTGCCGTTTAATGCC‑TGAAAAACATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGcc                                                                                                                  <  2:621081/142‑1 (MQ=255)                                         cGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                           >  2:1021362/1‑151 (MQ=255)                                             tttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  2:645828/1‑149 (MQ=255)                                              ttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                         >  2:257185/1‑148 (MQ=255)                                                  ttCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgc                                                                                                  >  2:329795/1‑151 (MQ=255)                                                         aaTCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                           >  2:93910/1‑151 (MQ=255)                                                           tttttttGCTGCCAAGGCTTTTTC‑AAGAACGGTTT‑AGATGTTTTTTTTTTCGTTTAATCCT‑TGAAAAAAATGCATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                              <  2:175417/141‑1 (MQ=255)                                                           tcttctTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                         >  1:1095997/1‑151 (MQ=255)                                                             ttcttGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                       >  2:877301/1‑151 (MQ=255)                                                               cttGCTGCGCAAGCGTTTGC‑AAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                       >  1:796909/1‑149 (MQ=255)                                                                 tgctgcGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                                     >  1:528855/1‑149 (MQ=255)                                                                         aaGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                           >  2:411459/1‑151 (MQ=255)                                                                          aagcttttC‑CAAAAAAGGTTAAGATGTTTTTTTTGTCGCTAAATGCT‑TGAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                <  2:614062/142‑1 (MQ=255)                                                                          aGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                             >  1:29645/1‑148 (MQ=255)                                                                            cGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATGATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                         >  2:195984/1‑150 (MQ=255)                                                                              ttttC‑CAAAACAGGTA‑AGATTATCTTTTTGCCTATTACTGCT‑TTTAAAACAGGTATGGCCAACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                               <  2:514668/142‑1 (MQ=255)                                                                              tttaC‑CAAAACGGTTT‑AGATGTTTTTTTTGTCGTTTAATGCC‑TAAAAAACGTGCATGCCCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                        <  1:787602/149‑1 (MQ=255)                                                                               tttC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                     >  2:39047/1‑151 (MQ=255)                                                                               ttctcaaaaaCAGGTT‑AGATATTTTTTTTGCCG‑TTAATTCCTTGAAAAACAGTCATGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                       <  1:150737/142‑1 (MQ=255)                                                                                 tC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                     >  1:96817/1‑149 (MQ=255)                                                                                      aaaaaGGTTAAGATGTTTTTTTTTTCTTTAAATCCC‑TAAAAAAAATGAAGGAGCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                    <  1:728059/143‑1 (MQ=255)                                                                                       aaaaGGTT‑AAATGATTTTTTTGTCGTTTAATCCT‑TAAAAAACATGCATGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                               <  1:147737/147‑1 (MQ=255)                                                                                       aaCAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                              >  2:302682/1‑151 (MQ=255)                                                                                        aCAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                              >  1:682118/1‑150 (MQ=255)                                                                                          aGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                           >  1:246087/1‑151 (MQ=255)                                                                                            gTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                         >  2:813151/1‑151 (MQ=255)                                                                                             tt‑AGATTTTTTTTTTGTCGTTAAATGCT‑TAAAAAACATGCAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                          <  2:717232/149‑1 (MQ=255)                                                                                                   tgatTTTTTTGTCGCTTAATGCT‑TGAAAAACATGCATGACCCAAAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                                    <  2:746155/150‑1 (MQ=255)                                                                                                      ttttttttTCGTTTAATCTC‑TAAAAAACATCAATGACACAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                                    <  2:33863/145‑1 (MQ=255)                                                                                                        tttttGTCTATAAATGCT‑AATAAAATATGAAGGACCAAAAAAAGAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                <  1:338535/149‑1 (MQ=255)                                                                                                        tttttGCCCCTAAATTCC‑TAAAAAATATACATGACCAACAAAATAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                               <  2:625693/150‑1 (MQ=255)                                                                                                               cgtttAATGCC‑TGAAAAACATGAATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                         <  1:296924/146‑1 (MQ=255)                                                                                                                 tttAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTACGTTGACTTTTAGCGCCCATATCTCCAGAATGCTGCCGTTTGTCAGAAATTCGCCGGCAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                       >  1:141081/2‑149 (MQ=255)                                                                                                                   tAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   >  2:363484/1‑151 (MQ=255)                                                                                                                     agtgctgcaaaaaCAGGAATGCGCCAAAAAATAAAAAAAAAACGCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                        <  2:302668/136‑1 (MQ=255)                                                                                                                     aggCC‑TGAAAAACATGAAGGACCAAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                  <  1:723198/148‑1 (MQ=255)                                                                                                                       gCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                >  1:631085/1‑150 (MQ=255)                                                                                                                        ccttgaaaaaCAAGAATGAACCAAAAAAAAAAAAAAAAACTTCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                  <  2:1085561/142‑1 (MQ=255)                                                                                                                         c‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                             >  2:85648/1‑151 (MQ=255)                                                                                                                              aaaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                          >  2:1136404/1‑150 (MQ=255)                                                                                                                              aaaaCATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                <  2:415755/144‑1 (MQ=255)                                                                                                                              aaaaCAGGCAGGCCCCAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                              <  1:394623/146‑1 (MQ=255)                                                                                                                               aaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                        >  2:1137046/1‑151 (MQ=255)                                                                                                                               aaaCATGAATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                        <  2:848496/151‑1 (MQ=255)                                                                                                                                      catgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  >  2:308029/1‑150 (MQ=255)                                                                                                                                      catgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                   >  2:810296/1‑149 (MQ=255)                                                                                                                                       atgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  >  2:552986/1‑149 (MQ=255)                                                                                                                                       atgACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  <  2:303532/149‑1 (MQ=255)                                                                                                                                              acaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat           >  2:781823/1‑149 (MQ=255)                                                                                                                                              aaaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat         <  2:156893/149‑1 (MQ=255)                                                                                                                                              aaaaaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat         <  2:1037826/149‑1 (MQ=255)                                                                                                                                              aaaaaaTAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata          <  1:854789/148‑1 (MQ=255)                                                                                                                                              aaaaaaTAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat           <  1:643421/147‑1 (MQ=255)                                                                                                                                               aaaaaaaATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt        <  1:329979/150‑1 (MQ=255)                                                                                                                                                aaaaaaATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAgtgagtga             <  1:416278/145‑1 (MQ=255)                                                                                                                                                aaaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc       >  1:1058882/1‑151 (MQ=255)                                                                                                                                                aaaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtc       >  2:236700/1‑151 (MQ=255)                                                                                                                                                   ataataAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct    <  1:1067761/151‑1 (MQ=255)                                                                                                                                                   aaaataAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTctct    <  1:111976/149‑1 (MQ=255)                                                                                                                                                     aataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  >  1:272519/1‑151 (MQ=255)                                                                                                                                                     aataAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg   <  1:80813/150‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                   TTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTC‑CAGAACAGGTT‑AGATGATCTTTTTGTCGCTTAATGCC‑TGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29347‑29642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑