Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	786,855	0	A	C	45.7%	120.8 / 19.2	82	G247G (GGT→GGG)	gpmA	phosphoglyceromutase 1
Reads supporting (aligned to +/- strand):  ref base A (35/9);  new base C (0/37);  total (35/47)
Fisher's exact test for biased strand distribution p-value = 7.57e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATG  >  NC_000913/786707‑787005                                                                                                                                                      |                                                                                                                                                       gTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTAcc                                                                                                                                                      >  1:444636/1‑151 (MQ=255)   cAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTAccc                                                                                                                                                     <  2:666306/150‑1 (MQ=255)   cAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCt                                                                                                                                                    >  2:489888/1‑151 (MQ=255)    agagTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTg                                                                                                                                                   >  1:1051203/1‑151 (MQ=255)    agagTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTg                                                                                                                                                   <  2:231528/151‑1 (MQ=255)     gagTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTgg                                                                                                                                                  <  2:633138/151‑1 (MQ=255)       gTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGtt                                                                                                                                                >  1:791776/1‑151 (MQ=255)                 cGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAAccgcc                                                                                                                                      <  2:499544/151‑1 (MQ=255)                      aaTCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCgctgc                                                                                                                                 >  2:270903/1‑151 (MQ=255)                       aTCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCgctgct                                                                                                                                >  2:1668/1‑151 (MQ=255)                       aTCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCgctgct                                                                                                                                >  1:446526/1‑151 (MQ=255)                               aGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGc                                                                                                                        >  1:724104/1‑151 (MQ=255)                                ggCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAAAAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGTTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGc                                                                                                                        <  2:867210/150‑1 (MQ=255)                                  ccTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGAt                                                                                                                     >  1:1128640/1‑151 (MQ=255)                                   cTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGAtc                                                                                                                    >  2:1045883/1‑151 (MQ=255)                                          gTCGCGCAGCGTCGCATCAGGCATTGTGCTCCATTGTTAGCAACAAAAAAG‑CCGCCTCACTTGCAGTCGGTTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTCCCTTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCa                                                                                                              <  1:583503/150‑1 (MQ=255)                                               gcAGCGTCGCATCGGCCAATGTGCTCCATTTTAAACAACAAAAAAG‑CCGCCTCCCTTGCGTCCGGCTTTCTCATTTAAACCGAATGCCTTTTCCTCCCCTTTCCCTTGTTTTGCACCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAt                                                                                                          <  1:150420/149‑1 (MQ=255)                                               gcAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAtt                                                                                                         >  1:614316/1‑150 (MQ=255)                                                 aGCGTCACCGCAGGCTATGTGCCCCATTGTAAGAAACAAAAAAG‑CCGTCTCATTTGCACTCGCCTTTTTCATTTAAACCTAATGACGTTTACTCCGCTTTCCCCTGGTTGGAACCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAtt                                                                                                         <  2:871512/148‑1 (MQ=255)                                                  gCGTCCCATCAGGCATTGTGCTCCTTTTTAAGCAAAAAAAAAG‑CCGACTCCCTTGCAGTCGGTTTCCTCATTTTAACCGAATGACTTTTTCTTCGCTTCACCCGGGTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCa                                                                                                              <  1:1064823/142‑1 (MQ=255)                                                     tCGCATCAGGCATTGTGCTCCATTGTTAGAAACAAAAAAG‑CCGACTCACTTGCAGTCGTCTTTCTCATTTAAAACGAATGTCGTTTACTTCCCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAg                                                                                                   <  1:633121/150‑1 (MQ=255)                                                     tCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTAccc                                                                                                     >  2:7721/1‑148 (MQ=255)                                                      cacaccAGGCATTGTGCCCCATTGTTAGAAAAAAAAAAG‑CCGCCCCACTTGCATTCGGTTTTCTCTTTTAAAACGAATGCCGTTTACTTCGTTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAt                                                                                                          <  2:589810/137‑1 (MQ=255)                                                       gCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGat                                                                                                 >  1:112492/1‑150 (MQ=255)                                                        cATCAGGCAATGTGCTCCATTGTTAACAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACTTTTACTTCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGata                                                                                                <  2:1104520/150‑1 (MQ=255)                                                          tcgggcatggtgCTCCTTTTTTAGAAAAAAAAAAC‑CCCACCCTCTTCCAGTGGTCTTTCTAATTTAAAAAGATGGACTTTTACTTCGTTTTCCCCTGGTTGAAAGCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCAtt                                                                                                         <  1:295268/130‑1 (MQ=255)                                                           cAGGAAATTTCCTCATTTGTTAAAAAAAAAAAAC‑CCGACTCCGTGGCAGTCGGCTTTCTCATTTTAAACGAATGATTTTTACTTCGTTTTCCCTTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAg                                                                                            <  1:1055262/151‑1 (MQ=255)                                                             gtaattgttctcttttGTTAACAAAAAAAAGG‑CCGTTTCTCTGCCACCGGGTTTCCTCAGTTAAACCGAAGGCCGTTTCCTCCGCTTTCCCCTGTTTTTCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCg                                                                                          <  2:1160499/137‑1 (MQ=255)                                                             ggcatggtccTCAATTGTAAGAAAAAAAAAGG‑CCGCCTCCCGTGCAGCCGGTTTTCTCATTTAAAGCTAATGATTTTTCCTACGCTTTCCCCGGTTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGc                                                                                           <  1:981255/141‑1 (MQ=255)                                                             ggCAATGTGCTCCATTGTTACCAACAAAAAAG‑CCGACTCACTTGCAGTCGGTTTTCTCATTTAAAACGAATGACGTTTCCTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCg                                                                                          <  2:800072/151‑1 (MQ=255)                                                             ggCAATGTCCTCAATTGTTACCAACAAAAAAG‑CCGACTCACTTCCAGCCGGCTTTCTCATTTAAAACGAATGGCTTTTTCTTCGCTTTCCCCTGTTTTCCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAg                                                                                            <  2:753150/149‑1 (MQ=255)                                                                aaTGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGttt                                                                                       >  1:791357/1‑151 (MQ=255)                                                                      cTCCATTGTTAGAAAAAAAAAAG‑CCGCCTCTCTTGCGGTCGGTTTTCTATTTTTAAACAAAGTACTTTTACTTCGCTTTCCCCTGTTTTCCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCg                                                                                  <  1:544074/150‑1 (MQ=255)                                                                        ccATTGTAGACAACAAAAAAG‑CCGCCTCCTTTCCAGCCGCTTTTCGATTTTAAAACAAAGGACGTTTTCTTCGCTTTCCCGTGTTTGGAACCCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGc                                                                                   <  1:623373/147‑1 (MQ=255)                                                                         cATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGttt                                                                              >  2:539227/1‑151 (MQ=255)                                                                          aTTGTTAGCAACAAAAAAG‑CCGCCTCTCTTGCAGTCGGCTTTCTCATTTAAAACGAATGTCTTTTACTTCTTTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCg                                                                                  <  1:267707/146‑1 (MQ=255)                                                                           ttGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTg                                                                             >  1:131349/1‑150 (MQ=255)                                                                           ttGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTg                                                                             >  1:912526/1‑150 (MQ=255)                                                                           ttGTTAGCAAAAAAAAAG‑CCGACTCCCTTGCAGTCGGTTTTCTAATTTAAACCAAATGACTTTTCCTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGttt                                                                              <  2:780443/149‑1 (MQ=255)                                                                           ttGTTAGCAAAAAAAAAC‑CCGACTCTCTTGCAGTCGGCTTTCTCATTTAAAACGAATGTCGTTTACTCCCCTTTCCCCTTGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGtt                                                                               <  2:805915/148‑1 (MQ=255)                                                                              ttAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAAt                                                                         >  1:163864/1‑151 (MQ=255)                                                                               tAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAAtt                                                                        >  1:1035551/1‑151 (MQ=255)                                                                               tAGCAACAAAAAAG‑CCGACTCACTTGCAGGCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAAtt                                                                        >  1:46341/1‑151 (MQ=255)                                                                                  caacaaAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATtctc                                                                     >  1:46701/1‑151 (MQ=255)                                                                                   aacaaAAAAG‑CCGACTCCCTTGCATTCGGCTTTCTCATTTAAAACGAATGACGTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATtct                                                                      <  2:73010/149‑1 (MQ=255)                                                                                   aaaaaaaaaC‑CGGCCTCCCTGGCAGCCGCTTTTCTCATTTTAAACGATTGACGTTTCCTCCGTTTTACCCTGGTTTCCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcg                                                                    <  2:601035/148‑1 (MQ=255)                                                                                    acaaAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACTTTTACTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATtct                                                                      <  2:410348/148‑1 (MQ=255)                                                                                    aaaaaaaaGCCCAACTCCCTTGCTGTCGCCTTTCTCATTTTAAACGAATGACGTTTTCTCCCCTTTCCCCTGTTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATtctc                                                                     <  1:782079/148‑1 (MQ=255)                                                                                     aaaaaaaC‑CCCACCCCCTGGCATTCGGTTTTCTCATTTTAAACGAATAACGTTTACTTCCTTTTCCCCTGGTTTCCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcgt                                                                   <  2:41638/149‑1 (MQ=255)                                                                                      aaaaaaG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcgtcg                                                                 >  1:1194792/1‑151 (MQ=255)                                                                                      aaaaaaG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcgtcg                                                                 >  2:1135763/1‑151 (MQ=255)                                                                                       aaaaaG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCtcgtcg                                                                 >  1:963486/1‑150 (MQ=255)                                                                                                   tCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACAcc                                                     >  1:1130902/1‑151 (MQ=255)                                                                                                       ttgctgttgtttttCACATTAAAAACGAATGTCTTTTCCTTCGTTTGCCCCTGTATGAAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACAcc                                                     <  1:1183999/136‑1 (MQ=255)                                                                                                          cAGTCGGTTTTCTCATTTAAAACGAATGACTTTTCCTTCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCgg                                                <  2:4481/149‑1 (MQ=255)                                                                                                                gCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGcc                                          >  1:874864/1‑149 (MQ=255)                                                                                                                   ttctattttaAAACGAATGCCTTTTCCTTCCCTTCCCCTTGTTTTCAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTc                                      <  1:699429/144‑1 (MQ=255)                                                                                                                   ttaccattgaaagggAAGGACGTTGACTTCGATTCGCCCGCGTTGGCGCCCGCCGCTGCTTTCGCTGCGATCTCGTGAGCATTGCCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACCCCAGTc                                      <  1:652560/136‑1 (MQ=255)                                                                                                                   ttCTCTTTTTAAACGAATAGTTTTTTCTCCGCTTTCCCCTGGTTGGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGt                                       <  2:1204963/149‑1 (MQ=255)                                                                                                                   ttCTCATTTTAAACAAATGACGTTTCCTTCGTTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTc                                      <  2:359440/150‑1 (MQ=255)                                                                                                                     ccaaTTTTAAAAAAGTGACTTTTATTCCGCTTTCCCCTGTTTGACAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGt                                       <  2:543024/144‑1 (MQ=255)                                                                                                                      tcttttcAAACTATTGGCGTTTCCTGCGCTTTCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTc                                      <  2:688375/144‑1 (MQ=255)                                                                                                                       cATTTAAAACGAGTGACTTTTCCTTCCTTTTCCCCTGTTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCgg                                    <  1:1084484/148‑1 (MQ=255)                                                                                                                        aTTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGat                                 >  1:1069863/1‑150 (MQ=255)                                                                                                                         ttttAAACGAATGACTTTTCCTTCGTTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCg                                     <  2:99946/145‑1 (MQ=255)                                                                                                                         tttaAAACGAATGCCTTTTCCTCCTCTTTCCCCTGTTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTc                                      <  1:821052/144‑1 (MQ=255)                                                                                                                           ttAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATAtt                              >  1:841602/1‑150 (MQ=255)                                                                                                                               aCGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCt                         >  2:729171/1‑151 (MQ=255)                                                                                                                                cGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTc                        >  1:278750/1‑151 (MQ=255)                                                                                                                                cGAATCACGTTTCCTTCGTTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTaa                            <  1:499838/147‑1 (MQ=255)                                                                                                                                  aaTGACGTTTACTTCGCTTTCCCCTGGTTGGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCa                       <  2:438243/150‑1 (MQ=255)                                                                                                                                    tGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCa                       >  2:111564/1‑148 (MQ=255)                                                                                                                                    tGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCa                       >  1:480459/1‑148 (MQ=255)                                                                                                                                         tttCCTCCGCTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGaa                   <  1:990145/147‑1 (MQ=255)                                                                                                                                         tttACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAAtctc               >  1:889503/1‑151 (MQ=255)                                                                                                                                         tttACTCCGCTTTACCCTGTTTTGAAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAAtct                <  1:762704/150‑1 (MQ=255)                                                                                                                                          ttACTCCGTTTTCCCCTGTTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAAtctc               <  2:272848/150‑1 (MQ=255)                                                                                                                                              ttCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTcttctt          >  1:891972/1‑151 (MQ=255)                                                                                                                                               tCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCttcttc         >  2:779366/1‑151 (MQ=255)                                                                                                                                                   tttCCCCTGTTTTCCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTcttctt          <  1:741510/146‑1 (MQ=255)                                                                                                                                                   tttCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCttcttc         <  1:574525/147‑1 (MQ=255)                                                                                                                                                   tttCCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCt      <  1:1081173/150‑1 (MQ=255)                                                                                                                                                      aCCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATg  >  2:1108582/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                       GTCAGAGTGGCGTATCCGATGAATCACCACAGGCCTGATAAGTCGCGCAGCGTCGCATCAGGCAATGTGCTCCATTGTTAGCAACAAAAAAG‑CCGACTCACTTGCAGTCGGCTTTCTCATTTTAAACGAATGACGTTTACTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATG  >  NC_000913/786707‑787005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑