| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 941,713 | 0 | T | G | 50.0% | 53.5 / 29.8 | 54 | V252G (GTG→GGG) | dmsA | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Reads supporting (aligned to +/- strand): ref base T (2/25); new base G (27/0); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGCCGGGCGCGAAGATGAGTGG‑ATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT > NC_000913/941569‑941862 | actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACtt < 2:249901/150‑1 (MQ=255)actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACt < 1:238760/149‑1 (MQ=255) cctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACt < 2:1091895/150‑1 (MQ=255) cctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACt < 1:345511/150‑1 (MQ=255) ccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTGATAACCCTGGCGAAACGGGAATAAGGCGCGGT‑GGGGGGaaaaa > 1:121672/1‑141 (MQ=255) gcgcAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGC‑GGGGGGGGTTACTAT‑Ctt > 2:294059/1‑150 (MQ=255) cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATATCCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAAc < 1:530638/151‑1 (MQ=255) cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAAc < 1:429878/151‑1 (MQ=255) aTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAgg < 2:754894/151‑1 (MQ=255) gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCa < 2:179822/150‑1 (MQ=255) cGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACg < 2:100016/151‑1 (MQ=255) aaGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCa < 1:767160/151‑1 (MQ=255) gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGG‑GGGGGGAATTACTATACTTAAACA‑GCACGCCCGaa > 1:1088462/1‑147 (MQ=255) aaCTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGCAAACAGTAAGCTGGTAGTGGTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGgg‑tgggtggtattagacattg > 1:61771/1‑117 (MQ=255) ataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAAt < 1:409830/149‑1 (MQ=255) ataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGc < 2:966376/151‑1 (MQ=255) ccTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCg < 1:1131964/151‑1 (MQ=255) cTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGAGTTATTAT‑TTTGAAAAAGGACGCCCGAAAACTAATGgcc > 2:401641/1‑149 (MQ=255) tACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGG‑GGGGGGGGTTAATTT‑CTTTTAAAAGCACACCCGAAAACTaa > 1:698067/1‑143 (MQ=255) cggcggCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATg < 2:698171/151‑1 (MQ=255) gcggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGat < 2:1186804/151‑1 (MQ=255) ggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatca < 2:1106947/151‑1 (MQ=255) cTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGC‑GGGGGGGGTTAATAT‑CTTATAAAAGAACGCCCGAAATATAATGCCCGccggaacat > 1:449090/1‑142 (MQ=255) tGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑TGGGGGGGTTCCCAT‑ATTTTACAGGCACGCCCAAAAACTAATTTCCGCCTGaac > 1:955794/1‑145 (MQ=255) gggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatca‑tca < 1:773554/150‑1 (MQ=255) cAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGA‑GGGGGGGCTTAATAT‑CTTCGACACGGACCCCAGAAATCCAATGCCCGcccgaccat > 1:478067/1‑137 (MQ=255) tGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGGTTACTAT‑CTTTAACAGGCACGCCCGAAAACTAATGCCCGCAAGATCA‑TCATCGATcc > 1:244048/1‑150 (MQ=255) gCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCcgc < 2:878658/150‑1 (MQ=255) aaCAGCCATTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCt < 1:987517/151‑1 (MQ=255) cAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCta < 1:804802/150‑1 (MQ=255) aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTAT‑TTTTAACAGGCACGCCAGAAAACTAATGGCCGCATGATCA‑TCATCGATCCGCGCtct > 1:1042876/1‑148 (MQ=255) gATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTAT‑CTTGAACAGGCACGCCAGAAAAATAATGCCCGCATGAGCA‑TCATTGGTTCCCGCCATACCGacaac > 2:276698/1‑148 (MQ=255) cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGcc < 2:155534/151‑1 (MQ=255) cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGATTACTTT‑CTTTAACAAGCACGCCAGAAATCTAATGCCCGCCTGATCA‑TCCTCGATCCCCGCCATACCGACA‑CCGGTGcc > 2:1230681/1‑151 (MQ=255) aaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGACCTTCTCT‑TTTGAACAAGCACGCCAGAAAAATAAATCCCCCCTGAGCA‑TCACCCACCCGCCGTATACCCCCACCCGGGGCCgg > 1:1189029/1‑150 (MQ=255) cAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGATTACTAT‑CTTTAACAGGCACGCCAGAAAACTAATGCCCGCATGATCC‑TCATCCATCCCCCCTATACCGACA‑CCGGTGCCgg > 2:1180169/1‑147 (MQ=255) aGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTAT‑CTTTAACAGGCACGCCCGAAATCTAATGCCCCCATGATCA‑TCATCGATCCGCACTATACCCACA‑CCCGGGCCGGgcg > 2:700142/1‑145 (MQ=255) cTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGACTT‑CTAT‑CTTTAACAAGCACACCCGCAATTTTATAGCCGCATGGTCATTAACCGAT‑CACGCCATACCCACA‑ACGGCGGCGGGCGCGCAGa > 2:935939/1‑149 (MQ=255) ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGC‑GGGGGGAATTTATCA‑CTTGAAAAGGCACGCCAGAAAAATAAATCCCCCCCGATCC‑TCATCGATTCGCGCTATACCGACA‑CCGGGGGCggggg > 2:636901/1‑139 (MQ=255) aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTAT‑CTTTAACAGGGACGCCAGAAATCTAATGCCCGCATGATCC‑TCATCGATCCGCGCTATAACGACA‑CCCGGGCCGGGCGCGGGGATGAgggg > 1:773500/1‑151 (MQ=255) gCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGA‑GGGGGGACCTACTAT‑ATTGAAAACGCGCGCCCCCAATCTAATGGCCCCCCGATGA‑TCATCGACGCCCGCTTTACCCTGA‑CCAGTGCCGGGGGCGgagagatgagg > 2:462430/1‑141 (MQ=255) gTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGCCGGGCGCGAAGATGAGTGG‑ATccc < 2:984027/150‑1 (MQ=255) gTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGG‑GGGGGGGGTTTCTAT‑CTTTAACAGGCACGCCAGAAAACTAATGCCCGCCTGATCA‑TTCTCCATACGCGCTCTAACCCCA‑CCGGTGTCGGGGGCGGAGATGAGTgggaccc > 1:1096768/1‑145 (MQ=255) tGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGCCGGGCGCGAAGATGAGTGG‑ATCCCTAt < 2:1096851/150‑1 (MQ=255) taaCCCTGGCGAAACGCGAATGAGTGGCGGTAGGGGGGAC‑TACTTT‑TTTTAAAAAGCAAGCCCGGAATCTAAATCCCCCATGGGCA‑TCATCGATCCCCGGCATAACGCCA‑CCCCGGCCGGGCGCCGAGATTAGAgggtacccactgttcc > 1:205134/1‑136 (MQ=255) aCCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTAT‑CTTGAACAAGCACGCCAGAAATCTAATGCCCGCCTGATCA‑TCATCGATCCGCGCCATACCGACA‑CCGGTGCCGGGGGCGAAGATGAGTGG‑GTCCCTATTCGTCCggg > 2:80979/1‑151 (MQ=255) cccTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGCCGGGCGCGAAGATGAGTGG‑ATCCCTATTCGTCCGGGa < 1:664128/151‑1 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTAT‑CTTTAAAAAGGACGCCAGAAAAATAATGCCCGCATGATCA‑TTATCGATCCGCGCTATACCCACA‑CCGGTGCCGGGGGCGGAGAAGAGGGG‑ATCCCCATTTCTCCgggga > 1:1076977/1‑147 (MQ=255) cGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTAT‑CTTGAAAAAGCACGCCAGAAATCTAAAGCCCGCATGATCC‑TCATCGATCCGCGCTATACCGACA‑CCGGGGCCGGGCGCGGAGATGAGTGG‑GTCCCCATTCGTCCcgg > 2:430199/1‑144 (MQ=255) ggaaCGCGAATGAGTGGCGGT‑GGGGGGGCTTACTAT‑CTTTAACAGGACCGCCCGAAAACTAATGACCGCCTGATCA‑TCATCGGTCCCCGCTATACCCACA‑CCGGCGCCGGGGGCGAAGATGAGGGG‑GTCCCTCTTCGTCCGGGGACAGATg > 1:203110/3‑151 (MQ=255) gAAACGCGAATGAGTGGCGGT‑GGGGGGACTTACTTT‑CTTTAAAAAGCACGCCAGAAAACTAATGGCCGCCTGATCA‑TCATCGCTCCGCGCCATACCCACA‑CCGGGGCCCGGCGCGGAGATGAGTGG‑ATCCCTATTCGTCCCGGGACAGAt > 1:557735/1‑150 (MQ=255) cgAATGAGTGGCGGG‑GGGGGGGCTTTATAA‑CTTTAAAAGGCACGCCAGAAAAATAAAGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGGGCCGGGGGCGAAAATGGGGGGCATCCC‑ATTCTTCCGGGGAAAGATGCCGc > 2:684775/1‑149 (MQ=255) cGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGCCGGGCGCGAAGATGAGTGG‑ATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGt > 1:456644/1‑151 (MQ=255) cGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGCCGGGCGCGAAGATGAGTGG‑ATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGt > 1:1245598/1‑151 (MQ=255) ggggTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGCCGGGCGCGAAGATGAGTGG‑ATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTgg < 1:891984/151‑1 (MQ=255) tGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGCCGGGCGCGAAGATGAGTGG‑ATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt > 1:930289/1‑150 (MQ=255) | ACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTAT‑CTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCA‑TCATCGATCCGCGCTATACCGACA‑CCGGTGCCGGGCGCGAAGATGAGTGG‑ATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT > NC_000913/941569‑941862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |