Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,245,247	0	G	T	40.3%	95.8 / 30.7	68	E30* (GAA→TAA)	ycgY	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (10/30);  new base T (26/1);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 2.06e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTC  >  NC_000913/1245102‑1245397                                                                                                                                                   |                                                                                                                                                       tCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGGTGCGCTTATTgtaa                                                                                                                                                     >  1:483046/1‑146 (MQ=255) tCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGTACa                                                                                                                                                    >  2:937607/1‑150 (MQ=255)      tCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAg                                                                                                                                               <  2:657243/150‑1 (MQ=255)        ccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAAt                                                                                                                                            <  2:1076253/151‑1 (MQ=255)         cGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGCGCTTATTTAAAAttt                                                                                                                                                 >  2:949690/1‑145 (MQ=255)             aacaacaacAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTAGCTGCGCTTATTTAACATTTAGAAtt                                                                                                                                           >  2:683190/1‑147 (MQ=255)              acaacaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCa                                                                                                                                      <  2:181370/151‑1 (MQ=255)               caacaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGCGCTTATTTAAAATTTAGAATTTATCa                                                                                                                                      >  2:719174/1‑150 (MQ=255)                 acaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGGTGCGCTTTTTTAAaatttataatttattca                                                                                                                                     >  1:902072/1‑146 (MQ=255)                  caacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATcc                                                                                                                                  <  2:279357/151‑1 (MQ=255)                   aacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGCGCTTTTTTTAAATTTTGAAATTATTAAtac                                                                                                                                  >  2:394007/1‑148 (MQ=255)                    acaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGCCCTTTTTTAACAATTTTAATTTATTAATCCtt                                                                                                                                >  2:418721/1‑151 (MQ=255)                    acaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCtt                                                                                                                                <  2:30578/151‑1 (MQ=255)                     caGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGCGCTTATTTAACATTTATAATTTATTAAACCtt                                                                                                                                >  2:773532/1‑150 (MQ=255)                     caGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTTAACATTTAGAATTGATCAATCCTTc                                                                                                                               <  1:898611/151‑1 (MQ=255)                       gAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGCGCTTATTTAAAATTTAGAATTTATTAATCCTTCt                                                                                                                              >  1:387765/1‑150 (MQ=255)                       gAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTg                                                                                                                             <  2:38288/151‑1 (MQ=255)                         aTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGCGCTTTTTTGACATTTAGAATTTGTCAATCCTTCttg                                                                                                                            >  2:452582/1‑148 (MQ=255)                         aTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGc                                                                                                                           <  1:1215578/151‑1 (MQ=255)                           ccTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCa                                                                                                                          <  1:905407/150‑1 (MQ=255)                                tttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGGCTG‑GCTTTTTTAAAATTTTGAATTTATCAATCCTTCTGGCAGAtt                                                                                                                      >  2:298251/1‑149 (MQ=255)                                   aaTTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGGTGCGCTTATTTAACATTTAGAAATGATCAATCCTTCTTGCAGATTAAGcc                                                                                                                 >  2:650718/1‑151 (MQ=255)                                    attattGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCa                                                                                                                <  1:830347/151‑1 (MQ=255)                                        ttGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTTAAAATTTTAAATTGATCAATACTTTTGGGAGATTTAACCaaaa                                                                                                             >  1:1150598/1‑150 (MQ=255)                                          gTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATAc                                                                                                          <  2:1091225/151‑1 (MQ=255)                                             tCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGGTGCGCTTTTTTAAAAATTAGAATTTATTAAAACTTCTGGCAGATTAAGCCaaaaaaacc                                                                                                        >  1:683095/1‑145 (MQ=255)                                               gttgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGtt                                                                                                     <  1:1236096/151‑1 (MQ=255)                                                 tgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGATTTTGACGAGCTTTTTATAAAATTAAAAATGATCAATTCTTATTGCGAAATTAGCTAAAATaatcca                                                                                                      >  1:178773/1‑143 (MQ=255)                                                    ttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGGGGTTATTTAAAAATTTAAATTTATAAAATCTTCTGGCAGATTTAAACAAAAAAcccccttgg                                                                                                  >  2:982839/1‑143 (MQ=255)                                                    ttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGc                                                                                                 <  2:325537/150‑1 (MQ=255)                                                     tttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGCGCTTTTTTAACATTTTGAATTTATCAATCCTTTTTGCAGATTAAGCCAAAAAAACCCTTTGGCa                                                                                                >  1:431017/1‑150 (MQ=255)                                                        ggTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGGTGCGGTTTTTGAAAAATTAGAATTGATAAATCCCTTTGGCAGATTAAGCCAAAAAAACCCTTTGGCaa                                                                                               >  2:975938/1‑148 (MQ=255)                                                         gtgtGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGCGCTTATTTAAAATTTAGAAATTATCAATCCTTCTTGCAGAATAAGCCAAAATACCCCTTCGGCAATggt                                                                                           >  1:896474/1‑149 (MQ=255)                                                            tgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGGTTTGGCTGCGCTTTTTTAAAATTTAGAATTGATCAATCCTTTTTGCAGATTAAGCCAAAAAACCCGGTTGGCaaaggtt                                                                                          >  1:228988/1‑144 (MQ=255)                                                                tCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑TGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGtt                                                                                     <  1:937810/150‑1 (MQ=255)                                                                  aGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGATTTTGGTGCGCCTTTTTGACAATTTGAATTTGTTAAACCTTCTTGCAGATTTAGACAAAATACCCGCTCGGGAATTCttttgctac                                                                                   >  2:906430/1‑143 (MQ=255)                                                                   gAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTAcgc                                                                                 <  2:762279/151‑1 (MQ=255)                                                                    aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCTGGGCTTAATGTACAATTAGAAATGTATAATCCTTCTTGCAGATTAAGCCAACAAAACCCTTTGGCAAAACTTCACTTAagc                                                                                 >  2:715317/1‑150 (MQ=255)                                                                      aataaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGaa                                                                              <  2:915842/151‑1 (MQ=255)                                                                       ataaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAag                                                                             <  2:796067/151‑1 (MQ=255)                                                                       ataaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAag                                                                             <  1:967697/151‑1 (MQ=255)                                                                        taaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGGCGCGCTTATTTAAAATTTAGAAATGATTAAACCTTCTGGGGGATTTAGCCAAAAAAACCGTTCGGCCATGCTTCCGTTAagcg                                                                                >  1:471153/1‑147 (MQ=255)                                                                          aataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGGTGCGCTTATTTAACATTTAGAATTTATCAATCCTTTTTGCAGAATAAGACAAAAAAACCGTTCGGCAATGCTTCCGTTAAGCGGag                                                                             >  2:518236/1‑148 (MQ=255)                                                                           atatTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGGTGCGGCTATTTAACAATTTGAATTTATCAATACTTCTTGCAGATTTAGCCAAAATACCCCGTCGGGAAAGCTTCCGTTACGGGGAGAgg                                                                          >  1:216901/1‑150 (MQ=255)                                                                               tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGTTGCGCTTTTTGAACATTTTGAATTTATAAAACCTTCTGGCAGATTAAACCAAAAAAACCGGTTGGCAAAGCTTTCGTTaaacggaaagggaagg                                                                     >  2:805692/1‑136 (MQ=255)                                                                                ttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAAAAATTAGAATTGATCAAACCTTCTGGCAGATTAAGCCAAAAAAACCGGTCGGCAATGCTTTAGTTAAGCGAAGAGGAATgg                                                                     >  1:150034/1‑150 (MQ=255)                                                                                ttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGATTTTGCTGGGCCTATTGAACATTTAAAAATTATCAAAACTTTTTGCAGATTTAGCCAAAATACCCCCTCGGCAATGCTTCAGTTACGCGAagag                                                                           >  1:1112787/1‑144 (MQ=255)                                                                                 tAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGGTGCGCTTATTGAACATTTAGAATTTATCAAACCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATgg                                                                     >  2:216030/1‑149 (MQ=255)                                                                                 tAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGCCGGGCTTCTTTAAAAATTAGAATTGATCAATCCTTCTGGCGGATTAAGCCAAAAAAACCGTTTGGCAATGCTTTAGTTACGCGAAGAGGAATgg                                                                     >  2:828853/1‑149 (MQ=255)                                                                                  aGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGGTGCGCTTATTTAACATTTAGAATTGATTAATCCTTCTTGCAGATTAAAACAAAATAACCGTTCGGCAAATCTTTAGTTACGCGaaaggggaagg                                                                     >  2:816386/1‑139 (MQ=255)                                                                                  aGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCt                                                                  <  1:325617/151‑1 (MQ=255)                                                                                   gACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTg                                                                 <  2:824688/151‑1 (MQ=255)                                                                                   gACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTg                                                                 <  1:1012112/151‑1 (MQ=255)                                                                                     cAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTTGGGGCGCTTATTTAACATTTAGAATTGATCAATCCTTCTGGGAGATTAAGGCAAAAAAACCCTTCGGGAATGCTTCAGTTTCGCGGAGAGGAATGGTCt                                                                  >  1:424520/1‑148 (MQ=255)                                                                                     cAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGaa                                                               <  2:845266/151‑1 (MQ=255)                                                                                                cAGGCTTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCttttttt                                                     <  2:1154115/150‑1 (MQ=255)                                                                                                       tAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTTTTTAACATTTAGAATTGATCAAACCTTCTGGCAGATTAAGCCAAAAAAACCGTTCGGCAATGCTTCAGTTACGCGAAGAGGGATGGTCTGAATTCTTTTTTttgttggt                                              >  2:974530/1‑148 (MQ=255)                                                                                                              ggggCGGATAGTGAGGC‑GGTTTTTGGTGCGCTTATTTAAAAATTTGAAATGATTAAACCTTTCGGCAGATTTAGCCCAAAAACCCCGTCGGCCATGCTTCCGGTTCGCCGAGAGGGAAGGGTTTAATTCTTTTTTTGGTTGGTaaaattt                                       >  2:233488/1‑146 (MQ=255)                                                                                                              ggggCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATtct                                       <  2:51410/150‑1 (MQ=255)                                                                                                                    gATAGTGAGTC‑GGTATTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAgt                                <  2:544599/151‑1 (MQ=255)                                                                                                                          gAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTgg                          <  1:719131/151‑1 (MQ=255)                                                                                                                          gAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTgg                          <  2:362896/151‑1 (MQ=255)                                                                                                                             gC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGAtt                       <  1:623453/151‑1 (MQ=255)                                                                                                                              c‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTa                      <  2:277989/151‑1 (MQ=255)                                                                                                                                 gTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtg                    <  1:566752/151‑1 (MQ=255)                                                                                                                                    ttGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgtg                  <  2:840530/150‑1 (MQ=255)                                                                                                                                     tGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGat                >  1:1091076/1‑151 (MQ=255)                                                                                                                                        cTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGattat             >  1:210701/1‑151 (MQ=255)                                                                                                                                          gcgcTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGattatt            >  2:829211/1‑150 (MQ=255)                                                                                                                                                   gAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTc  <  2:239202/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                       TCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGC‑GGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAACTC  >  NC_000913/1245102‑1245397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑