Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,657	0	A	G	43.8%	113.7 / 25.0	81	F386S (TTC→TCC)	yhfW	phosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (9/36);  new base G (35/0);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 1.24e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGC  >  NC_000913/3508516‑3508805                                                                                                                                                |                                                                                                                                                     gttgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCCC‑GC‑GGgaaaaat                                                                                                                                                >  2:826493/1‑146 (MQ=255)  ttgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTcac                                                                                                                                             <  2:1161268/150‑1 (MQ=255)    gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTcacac                                                                                                                                           <  2:578483/150‑1 (MQ=255)    gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGCGG‑GC‑GGGAAAAtttaaac                                                                                                                                           >  2:855615/1‑145 (MQ=255)        ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGG‑GC‑GGCAAAAATaacaaa                                                                                                                                          >  1:503920/1‑145 (MQ=255)        ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTacacacag                                                                                                                                       >  2:1190874/1‑148 (MQ=255)        ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTACAAACgg                                                                                                                                       >  2:103190/1‑150 (MQ=255)        ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGG‑GCAGGAAAAATt‑acaca                                                                                                                                          >  1:356010/1‑147 (MQ=255)           aaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATcaaa                                                                                                                                            >  2:1206299/1‑140 (MQ=255)               cGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACACACGGGAGccccc                                                                                                                               >  2:1151980/1‑151 (MQ=255)               cGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGccccc                                                                                                                               <  2:354840/151‑1 (MQ=255)               cGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGccccc                                                                                                                               <  2:298873/151‑1 (MQ=255)                 gagagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAATTCAAACAAAGGACCCCCcac                                                                                                                             >  1:1205479/1‑151 (MQ=255)                   gagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGG‑GC‑GGGAAAAATTACACACGGGAGccccccc                                                                                                                             >  2:736745/1‑147 (MQ=255)                   gagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACAt                                                                                                                           <  2:315069/151‑1 (MQ=255)                     gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACAAAAGGCAGgcccccc                                                                                                                             >  1:1008133/1‑145 (MQ=255)                         aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATAACAAACGGGAGCCCCCCCATCAGaa                                                                                                                      >  1:872479/1‑150 (MQ=255)                           tatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGC‑GC‑GGGAAAAATCACACCCGGTTGCCACCACATCAGaa                                                                                                                      >  2:21667/1‑148 (MQ=255)                             tGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGG‑CC‑GGGGAAAATCAAACAAACTACCCCCCCCATCaccaa                                                                                                                     >  1:836412/1‑143 (MQ=255)                                aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAAATAAACAAGGTAGACCCCCCCTCAGaaaaagcggg                                                                                                              >  1:100759/1‑144 (MQ=255)                                aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtggt                                                                                                              <  1:1201674/151‑1 (MQ=255)                                  aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTgc                                                                                                            <  2:1082202/151‑1 (MQ=255)                                      tCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACACACGGTAGCCCCCCCAACAGAAAACGTGGGgcg                                                                                                           >  2:100209/1‑148 (MQ=255)                                       cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGcaca                                                                                                       <  1:303997/151‑1 (MQ=255)                                            gCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACAAAAGGGAGCCCCCCCAACAGAAAgaggggggggcccac                                                                                                      >  1:1108920/1‑133 (MQ=255)                                            gCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAg                                                                                                  <  1:659663/151‑1 (MQ=255)                                              gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGCAAAGAGCGACCATTTTGTGGCGGCCGGGC‑GC‑GGAAAAAATCACACAAGGGAGCACCCCCCTCACAAAACGTGGGGGGCCCAccc                                                                                                    >  1:880635/1‑146 (MQ=255)                                              gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACACACGGGAGCCCCCCCATCAGAAAGCGTGGGGCGCACAcc                                                                                                     >  2:454303/1‑146 (MQ=255)                                                 ggTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACACACGGGAGGCCCCACATCAGAAAAAGTGGGGCGCCCACCGAGCt                                                                                                >  2:556219/1‑148 (MQ=255)                                                  gtgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGt                                                                                            <  2:615736/151‑1 (MQ=255)                                                   tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGGG‑GGAAAAATTAAAAAAC‑GAAGACCCCCCATCAGAAAACGGGGGGCGCACACCCAgcggcg                                                                                             >  2:1027381/1‑149 (MQ=255)                                                    gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTa                                                                                           <  2:612703/150‑1 (MQ=255)                                                      aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCg                                                                                        <  2:701107/151‑1 (MQ=255)                                                         ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACACACGGTAGGCCCCCCATCAGAAAACGGGGTGCGCACACCGAGCTGCGTAGCGatc                                                                                     >  2:378368/1‑151 (MQ=255)                                                         ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                                                                     <  2:1219998/151‑1 (MQ=255)                                                             cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGG‑GC‑GGGAAAAAATACAAACGGGAGCCCCCACCACCAAAAGCGGGGGGCGCACACCCCGCTGCGGAGGGGTCATcc                                                                                 >  1:392068/1‑151 (MQ=255)                                                             cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACCGGAGCCCCCCCACCAGAAAACGGGGGGGGCCCACCCAGTTGCGGAGCGatccccc                                                                                 >  1:444352/1‑147 (MQ=255)                                                               gATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACAAACGGCAGCCCCCACAACAGAAAGCGGGGGGGGCACACCCAGCTGCGTAGCGGTCATccc                                                                                >  2:976230/1‑150 (MQ=255)                                                                  cAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTg                                                                             <  2:63232/150‑1 (MQ=255)                                                                    aaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACGGTAACCCCCCCAACACAAAACGGGGGGGGCACACCGAGCTGCGTGGCGGGCATCCCCTGCt                                                                           >  1:742615/1‑150 (MQ=255)                                                                     aaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGa                                                                         <  2:616779/151‑1 (MQ=255)                                                                     aaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGa                                                                         <  1:384089/151‑1 (MQ=255)                                                                        tCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGC‑GC‑GGGAAAAATCACACACCGGAGACCCCACATCAGAAAGCGTGGGGCGCCCACCCAGCCGCGTAGCGATCATCCCTTTCTGGTa                                                                       >  2:487714/1‑150 (MQ=255)                                                                          aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGC‑GC‑GGGAAAATTTACAAACGGGAGCTCCCCCACCAGAAAAAGAGGGGCGCAAACCGAACTGCCGAGCGAACCTCCCCTGCTTATaaa                                                                     >  2:1240219/1‑150 (MQ=255)                                                                            aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGG‑GC‑GGGAAAAATTACACACGGTAGCCCCCACATCAAGAAACGGGGGGGGCACACCGAGACGCGTAGCGGGCATCCCCTTCTGATaaaa                                                                    >  2:1242128/1‑148 (MQ=255)                                                                            aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAcc                                                                   <  1:545454/150‑1 (MQ=255)                                                                                tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTAAAAACGGGAGGCCCCCCATCCCAAAACGGGGGGCGCACACCCAGCTGCGGAGCGGTCATCCCCTTCTGATAAAACAacac                                                              >  1:1181790/1‑151 (MQ=255)                                                                                tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGAGG‑GC‑GGAAAAAAATACACACGGTAACCCCCCCATCAGAAAACGGGGGGCGCGCAACCAGCTGCGATGCGATCATCCCTTGCTGTTAAAacaaca                                                               >  2:694414/1‑150 (MQ=255)                                                                                     ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTg                                                            <  2:1074788/148‑1 (MQ=255)                                                                                      cTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGG‑GC‑GGGAAAAATCAAACACGGTAACCCCCCCATCAGAAAAGGGGGGGGGCACACCGAGCAGCGGAGCGATCATCCCTTCCTGATAAAACAACACTGGcac                                                        >  2:593348/1‑151 (MQ=255)                                                                                         cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACGGGAGCCCCCCCCTCAAAAAACGGGGGGCGCACACCCAGCTGCGGAGCGATCATCCCCTTCTGGTAAAACAACACCGGcacc                                                       >  2:582726/1‑149 (MQ=255)                                                                                            aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                  <  1:918523/151‑1 (MQ=255)                                                                                            aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                  <  1:227418/151‑1 (MQ=255)                                                                                             ccGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGG‑GG‑GGGAAAAATTAAACACGGGGGCCCCCACAACAAAAAGAGGGGGGGGCACACCGAGCGGCCTTGCGGTCAACCCCTGCTGGTaaaac                                                                   >  2:613112/1‑131 (MQ=255)                                                                                                gagggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCgg                                              <  1:258265/151‑1 (MQ=255)                                                                                                     aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACAAACCGCAGCCCCCCCATCAGAAAGCGCGGGGGGCCCACCCCGCTGCGTAGCGATCATCCCCTTGTTTTAAACCACCACTCGCACCCCCTCCCGGGGAt                                          >  1:34653/1‑150 (MQ=255)                                                                                                     aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACCGGAGCCCCCACACCAAAAAGCGGGGTGCGGACACCGAGCTGCGGTGGGATCATTCCCTCCTGAGCAAACAACACTAGCACCACCTcgc                                                >  1:1222893/1‑144 (MQ=255)                                                                                                       gaaagaaaAGAGCGACCATTTTGTGGCGGTGGGGG‑GC‑GGGAAAAATTACAAACGGGAGCCCCCACATCAGAAAACGGGGGGGGCCCACCCAGCTGCGGAGCGGTCATCCCCTGCTGATAAAACAGCACCGGCACCCCTTCGCGGGGAtgg                                        >  1:534926/1‑150 (MQ=255)                                                                                                        aaagaaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtggtg                                      <  1:982977/151‑1 (MQ=255)                                                                                                         aagaaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtggtgg                                     <  2:1012575/151‑1 (MQ=255)                                                                                                            aaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACACACAGGAGCCCCCCCAACAAAAAGCGGGGGGCGCACACCCAGCTGCGTAGCGATCATCCCCTGCTGATAAAACAACACCGGCACCACCTCCCGGGGAtggtgggt                                   >  2:547436/1‑148 (MQ=255)                                                                                                            aaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtg                                  <  1:740491/151‑1 (MQ=255)                                                                                                            aaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtg                                  <  1:108936/151‑1 (MQ=255)                                                                                                              aagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtgtg                                <  2:642049/151‑1 (MQ=255)                                                                                                              aagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtgtg                                <  1:424970/151‑1 (MQ=255)                                                                                                                gagCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAACTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGAc                              <  1:1208247/151‑1 (MQ=255)                                                                                                                   cGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACCGTAGCCCCCACATCAGAAAGCGGGGGGCGCACACCGAGCTGCGTAGCGATCATCCCCTTCTGATAAAACAGCACTGGCACCACTTCGCGGGGATGGTGGCTGTGAcc                             >  2:59997/1‑149 (MQ=255)                                                                                                                   cGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCaa                           <  1:53783/151‑1 (MQ=255)                                                                                                                     aCCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATg                         <  2:534952/151‑1 (MQ=255)                                                                                                                           ttGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCgg                    <  1:454282/150‑1 (MQ=255)                                                                                                                            tgtgGCGGTGGCGC‑GC‑GGGAAAAATCACACACGGGAGCCCCCCCATCAGAAAGCGTGGGGCGCCCACCGCGCTGCGTAGCGATCATCCCTTGCTGATAAAACCACACTGGCACCACCTCCCGGGGATGGTGGCTGTGACCAATGGGCgg                    >  2:1113071/1‑149 (MQ=255)                                                                                                                            tgtgGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGa                   <  1:855569/150‑1 (MQ=255)                                                                                                                             gtggcggtggggC‑GC‑GGGAAAAATCACACACGGGTGCCCCCCCATCAGAAAGCGGGGGGCGCACACCGCGCTGCGCAGCGATCATCCCTTGCTGATAAACAAGCACTGGGACCCCCTCTCGGGGATGGTGGGTGTGACCAAAGGGCgg                    >  1:795227/1‑148 (MQ=255)                                                                                                                             gtggcggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATc                 <  1:208701/151‑1 (MQ=255)                                                                                                                               ggcggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCTCTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg                <  1:1008726/150‑1 (MQ=255)                                                                                                                               ggcggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGt               <  2:404953/151‑1 (MQ=255)                                                                                                                                 cggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg             >  2:283646/1‑151 (MQ=255)                                                                                                                                 cggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg             >  1:380026/1‑151 (MQ=255)                                                                                                                                      gcgc‑gc‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCg          <  1:820107/149‑1 (MQ=255)                                                                                                                                           gc‑gGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc    >  1:1240898/1‑151 (MQ=255)                                                                                                                                            c‑gGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg   >  1:300289/1‑151 (MQ=255)                                                                                                                                              ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGc  >  2:1243790/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                     GTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGC  >  NC_000913/3508516‑3508805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑