Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,662	0	T	A	43.8%	116.0 / 37.7	81	E384D (GAA→GAT)	yhfW	phosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (9/36);  new base A (35/0);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 1.24e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAC  >  NC_000913/3508517‑3508811                                                                                                                                                    |                                                                                                                                                      ttgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTcac                                                                                                                                                   <  2:1161268/150‑1 (MQ=255)   gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTcacac                                                                                                                                                 <  2:578483/150‑1 (MQ=255)   gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGCGG‑GC‑GGGAAAAtttaaac                                                                                                                                                 >  2:855615/1‑145 (MQ=255)       ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGG‑GC‑GGCAAAAATaacaaa                                                                                                                                                >  1:503920/1‑145 (MQ=255)       ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTacacacag                                                                                                                                             >  2:1190874/1‑148 (MQ=255)       ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATTACAAACgg                                                                                                                                             >  2:103190/1‑150 (MQ=255)       ttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGG‑GCAGGAAAAATt‑acaca                                                                                                                                                >  1:356010/1‑147 (MQ=255)          aaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATcaaa                                                                                                                                                  >  2:1206299/1‑140 (MQ=255)              cGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACACACGGGAGccccc                                                                                                                                     >  2:1151980/1‑151 (MQ=255)              cGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGccccc                                                                                                                                     <  2:354840/151‑1 (MQ=255)              cGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGccccc                                                                                                                                     <  2:298873/151‑1 (MQ=255)                gagagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAATTCAAACAAAGGACCCCCcac                                                                                                                                   >  1:1205479/1‑151 (MQ=255)                  gagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGG‑GC‑GGGAAAAATTACACACGGGAGccccccc                                                                                                                                   >  2:736745/1‑147 (MQ=255)                  gagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACAt                                                                                                                                 <  2:315069/151‑1 (MQ=255)                    gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACAAAAGGCAGgcccccc                                                                                                                                   >  1:1008133/1‑145 (MQ=255)                        aaTATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATAACAAACGGGAGCCCCCCCATCAGaa                                                                                                                            >  1:872479/1‑150 (MQ=255)                          tatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGC‑GC‑GGGAAAAATCACACCCGGTTGCCACCACATCAGaa                                                                                                                            >  2:21667/1‑148 (MQ=255)                            tGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGG‑CC‑GGGGAAAATCAAACAAACTACCCCCCCCATCaccaa                                                                                                                           >  1:836412/1‑143 (MQ=255)                               aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAAATAAACAAGGTAGACCCCCCCTCAGaaaaagcggg                                                                                                                    >  1:100759/1‑144 (MQ=255)                               aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCgtggt                                                                                                                    <  1:1201674/151‑1 (MQ=255)                                 aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTgc                                                                                                                  <  2:1082202/151‑1 (MQ=255)                                     tCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACACACGGTAGCCCCCCCAACAGAAAACGTGGGgcg                                                                                                                 >  2:100209/1‑148 (MQ=255)                                      cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGcaca                                                                                                             <  1:303997/151‑1 (MQ=255)                                           gCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACAAAAGGGAGCCCCCCCAACAGAAAgaggggggggcccac                                                                                                            >  1:1108920/1‑133 (MQ=255)                                           gCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAg                                                                                                        <  1:659663/151‑1 (MQ=255)                                             gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGCAAAGAGCGACCATTTTGTGGCGGCCGGGC‑GC‑GGAAAAAATCACACAAGGGAGCACCCCCCTCACAAAACGTGGGGGGCCCAccc                                                                                                          >  1:880635/1‑146 (MQ=255)                                             gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACACACGGGAGCCCCCCCATCAGAAAGCGTGGGGCGCACAcc                                                                                                           >  2:454303/1‑146 (MQ=255)                                                ggTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACACACGGGAGGCCCCACATCAGAAAAAGTGGGGCGCCCACCGAGCt                                                                                                      >  2:556219/1‑148 (MQ=255)                                                 gtgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGt                                                                                                  <  2:615736/151‑1 (MQ=255)                                                  tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGGG‑GGAAAAATTAAAAAAC‑GAAGACCCCCCATCAGAAAACGGGGGGCGCACACCCAgcggcg                                                                                                   >  2:1027381/1‑149 (MQ=255)                                                   gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTa                                                                                                 <  2:612703/150‑1 (MQ=255)                                                     aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCg                                                                                              <  2:701107/151‑1 (MQ=255)                                                        ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACACACGGTAGGCCCCCCATCAGAAAACGGGGTGCGCACACCGAGCTGCGTAGCGatc                                                                                           >  2:378368/1‑151 (MQ=255)                                                        ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                                                                           <  2:1219998/151‑1 (MQ=255)                                                            cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGG‑GC‑GGGAAAAAATACAAACGGGAGCCCCCACCACCAAAAGCGGGGGGCGCACACCCCGCTGCGGAGGGGTCATcc                                                                                       >  1:392068/1‑151 (MQ=255)                                                            cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACCGGAGCCCCCCCACCAGAAAACGGGGGGGGCCCACCCAGTTGCGGAGCGatccccc                                                                                       >  1:444352/1‑147 (MQ=255)                                                              gATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACAAACGGCAGCCCCCACAACAGAAAGCGGGGGGGGCACACCCAGCTGCGTAGCGGTCATccc                                                                                      >  2:976230/1‑150 (MQ=255)                                                                 cAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTg                                                                                   <  2:63232/150‑1 (MQ=255)                                                                   aaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACGGTAACCCCCCCAACACAAAACGGGGGGGGCACACCGAGCTGCGTGGCGGGCATCCCCTGCt                                                                                 >  1:742615/1‑150 (MQ=255)                                                                    aaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGa                                                                               <  1:384089/151‑1 (MQ=255)                                                                    aaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGa                                                                               <  2:616779/151‑1 (MQ=255)                                                                       tCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGC‑GC‑GGGAAAAATCACACACCGGAGACCCCACATCAGAAAGCGTGGGGCGCCCACCCAGCCGCGTAGCGATCATCCCTTTCTGGTa                                                                             >  2:487714/1‑150 (MQ=255)                                                                         aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGC‑GC‑GGGAAAATTTACAAACGGGAGCTCCCCCACCAGAAAAAGAGGGGCGCAAACCGAACTGCCGAGCGAACCTCCCCTGCTTATaaa                                                                           >  2:1240219/1‑150 (MQ=255)                                                                           aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGG‑GC‑GGGAAAAATTACACACGGTAGCCCCCACATCAAGAAACGGGGGGGGCACACCGAGACGCGTAGCGGGCATCCCCTTCTGATaaaa                                                                          >  2:1242128/1‑148 (MQ=255)                                                                           aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAcc                                                                         <  1:545454/150‑1 (MQ=255)                                                                               tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTAAAAACGGGAGGCCCCCCATCCCAAAACGGGGGGCGCACACCCAGCTGCGGAGCGGTCATCCCCTTCTGATAAAACAacac                                                                    >  1:1181790/1‑151 (MQ=255)                                                                               tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGAGG‑GC‑GGAAAAAAATACACACGGTAACCCCCCCATCAGAAAACGGGGGGCGCGCAACCAGCTGCGATGCGATCATCCCTTGCTGTTAAAacaaca                                                                     >  2:694414/1‑150 (MQ=255)                                                                                    ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTg                                                                  <  2:1074788/148‑1 (MQ=255)                                                                                     cTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGG‑GC‑GGGAAAAATCAAACACGGTAACCCCCCCATCAGAAAAGGGGGGGGGCACACCGAGCAGCGGAGCGATCATCCCTTCCTGATAAAACAACACTGGcac                                                              >  2:593348/1‑151 (MQ=255)                                                                                        cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACGGGAGCCCCCCCCTCAAAAAACGGGGGGCGCACACCCAGCTGCGGAGCGATCATCCCCTTCTGGTAAAACAACACCGGcacc                                                             >  2:582726/1‑149 (MQ=255)                                                                                           aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                        <  1:918523/151‑1 (MQ=255)                                                                                           aCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTc                                                        <  1:227418/151‑1 (MQ=255)                                                                                            ccGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGG‑GG‑GGGAAAAATTAAACACGGGGGCCCCCACAACAAAAAGAGGGGGGGGCACACCGAGCGGCCTTGCGGTCAACCCCTGCTGGTaaaac                                                                         >  2:613112/1‑131 (MQ=255)                                                                                               gagggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCgg                                                    <  1:258265/151‑1 (MQ=255)                                                                                                    aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGC‑GC‑GGGAAAAATCACAAACCGCAGCCCCCCCATCAGAAAGCGCGGGGGGCCCACCCCGCTGCGTAGCGATCATCCCCTTGTTTTAAACCACCACTCGCACCCCCTCCCGGGGAt                                                >  1:34653/1‑150 (MQ=255)                                                                                                    aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACCGGAGCCCCCACACCAAAAAGCGGGGTGCGGACACCGAGCTGCGGTGGGATCATTCCCTCCTGAGCAAACAACACTAGCACCACCTcgc                                                      >  1:1222893/1‑144 (MQ=255)                                                                                                      gaaagaaaAGAGCGACCATTTTGTGGCGGTGGGGG‑GC‑GGGAAAAATTACAAACGGGAGCCCCCACATCAGAAAACGGGGGGGGCCCACCCAGCTGCGGAGCGGTCATCCCCTGCTGATAAAACAGCACCGGCACCCCTTCGCGGGGAtgg                                              >  1:534926/1‑150 (MQ=255)                                                                                                       aaagaaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtggtg                                            <  1:982977/151‑1 (MQ=255)                                                                                                        aagaaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtggtgg                                           <  2:1012575/151‑1 (MQ=255)                                                                                                           aaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATTACACACAGGAGCCCCCCCAACAAAAAGCGGGGGGCGCACACCCAGCTGCGTAGCGATCATCCCCTGCTGATAAAACAACACCGGCACCACCTCCCGGGGAtggtgggt                                         >  2:547436/1‑148 (MQ=255)                                                                                                           aaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtg                                        <  1:108936/151‑1 (MQ=255)                                                                                                           aaaagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtg                                        <  1:740491/151‑1 (MQ=255)                                                                                                             aagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtgtg                                      <  2:642049/151‑1 (MQ=255)                                                                                                             aagaGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCtgtg                                      <  1:424970/151‑1 (MQ=255)                                                                                                               gagCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAACTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGAc                                    <  1:1208247/151‑1 (MQ=255)                                                                                                                  cGACCATTTTGTGGCGGTGGCGC‑GC‑GGGAAAAATCACACACCGTAGCCCCCACATCAGAAAGCGGGGGGCGCACACCGAGCTGCGTAGCGATCATCCCCTTCTGATAAAACAGCACTGGCACCACTTCGCGGGGATGGTGGCTGTGAcc                                   >  2:59997/1‑149 (MQ=255)                                                                                                                  cGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCaa                                 <  1:53783/151‑1 (MQ=255)                                                                                                                    aCCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATg                               <  2:534952/151‑1 (MQ=255)                                                                                                                          ttGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCgg                          <  1:454282/150‑1 (MQ=255)                                                                                                                           tgtgGCGGTGGCGC‑GC‑GGGAAAAATCACACACGGGAGCCCCCCCATCAGAAAGCGTGGGGCGCCCACCGCGCTGCGTAGCGATCATCCCTTGCTGATAAAACCACACTGGCACCACCTCCCGGGGATGGTGGCTGTGACCAATGGGCgg                          >  2:1113071/1‑149 (MQ=255)                                                                                                                           tgtgGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGa                         <  1:855569/150‑1 (MQ=255)                                                                                                                            gtggcggtggggC‑GC‑GGGAAAAATCACACACGGGTGCCCCCCCATCAGAAAGCGGGGGGCGCACACCGCGCTGCGCAGCGATCATCCCTTGCTGATAAACAAGCACTGGGACCCCCTCTCGGGGATGGTGGGTGTGACCAAAGGGCgg                          >  1:795227/1‑148 (MQ=255)                                                                                                                            gtggcggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATc                       <  1:208701/151‑1 (MQ=255)                                                                                                                              ggcggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCTCTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg                      <  1:1008726/150‑1 (MQ=255)                                                                                                                              ggcggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGt                     <  2:404953/151‑1 (MQ=255)                                                                                                                                cggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg                   >  1:380026/1‑151 (MQ=255)                                                                                                                                cggtggcgC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg                   >  2:283646/1‑151 (MQ=255)                                                                                                                                     gcgc‑gc‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCg                <  1:820107/149‑1 (MQ=255)                                                                                                                                          gc‑gGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc          >  1:1240898/1‑151 (MQ=255)                                                                                                                                           c‑gGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg         >  1:300289/1‑151 (MQ=255)                                                                                                                                             ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGc        >  2:1243790/1‑151 (MQ=255)                                                                                                                                                   aTTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAc  <  2:654832/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGC‑GC‑GGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAC  >  NC_000913/3508517‑3508811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑