| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,875 | 0 | A | T | 40.5% | 43.9 / 13.8 | 37 | N272K (AAT→AAA) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base A (17/5); new base T (0/15); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.12e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTA > NC_000913/357727‑358019 | tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTAtt > 1:118940/1‑151 (MQ=255) ggCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTccgccg < 1:243324/150‑1 (MQ=255) ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGca > 1:920564/1‑150 (MQ=255) ctaaaaaGCGTAGCGCATCAGGCTATTCCACCCGCAGTCCTGGCTCCGCGCCTTGCTTGATCCATTTCCGCCAACACCCGCGCATTGTTCCACCGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCTGCTGTCTGCCAGCTATTTTTCCGCCGca < 1:1172270/144‑1 (MQ=17) gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca < 2:659290/150‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GAGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaa > 1:477322/1‑147 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc > 1:1100999/1‑151 (MQ=255) tAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgccg > 2:31148/1‑151 (MQ=255) aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGt > 1:919355/1‑151 (MQ=255) cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctc > 1:725895/1‑151 (MQ=255) cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctc > 2:783481/1‑151 (MQ=255) aTCAGGCAATGCCAGCCGCAGACCTGAGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGACGTtct > 1:355597/1‑149 (MQ=255) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gg > 1:190301/1‑151 (MQ=255) ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gggg > 2:814014/1‑151 (MQ=255) ccgtgtgCCGGCGTCTCCGGTGATTTTTTCCCCCAAACACCCGCCCTTTTACCCAACCCCATG‑GCAAAAAGTCCCTC‑C‑GCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAag < 2:1219728/146‑1 (MQ=255) ccTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGCGGC‑ggggg > 1:5610/1‑144 (MQ=255) tgtgTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑ggggggacagg > 1:693371/1‑142 (MQ=255) ctgCTACGGTATTTCTTTCCCGCCAACACCCCCGCTTTTACCCAACCCCTGGTG‑AAAAA‑AGCCTTCC‑GCGGGGGCTTTCTCCAGGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAAt < 2:731707/142‑1 (MQ=255) ctccggtgtTTCTTTCCCGCCAACACCCGCCCTTTTACCCACCGCCTTGTGAAAAAA‑TCCCTC‑C‑GGGGCGCTTGTCTCCCAGCTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAg < 2:836702/145‑1 (MQ=255) aCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAGaaaaaaaattaaaagc > 2:585065/1‑138 (MQ=25) attttCATCCCCCCCACCACCCGCCCTTTCACCCACCGCATTGTCAAAAAC‑GGCCTC‑C‑GGGGCGCCTGTCTCCCAGTTTTTTTCCCGCCACAAAAAAGCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGc < 1:247189/142‑1 (MQ=255) taaatcccccccAACACCCGCGCTTTTACCCAACGCCATGTGAAAAAA‑T‑CCTT‑CGGGGGGGGCTGTCTGCCAGTTTTTTTTCCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCAt < 2:431267/139‑1 (MQ=255) cATTCCCCCCACCACCCGCGCATTTCTCCAACGCAATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTTTTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgtt < 1:1141385/150‑1 (MQ=255) caacCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGCAGAGAAATAAATTTTAACCCCGCCTGTTTTGTgg > 2:502570/1‑144 (MQ=25) aTTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAAAGAAAAAAGCTTTAACACCG‑CCTGTTTTGTgggggagtct > 2:39833/1‑135 (MQ=25) caacccccATGTAAAAAAA‑TCCCTC‑C‑GGGGGGCCTGCCTCCCATTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc < 1:918972/140‑1 (MQ=255) ccATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGAGAAAAAAATTTTAAATCCCCATGTTGTGTGGGAATAGCTGCTGGGaagcaagagggaa > 2:491663/1‑139 (MQ=25) ccATGTAAAAAAA‑TCCTTC‑C‑GGGGCGGTTGTCTCCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAg < 2:625501/151‑1 (MQ=255) attcccTC‑C‑GGGGCGGCGTTCGGCCGGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggc < 1:308755/147‑1 (MQ=255) acgCCTT‑C‑GCGGCGCGTGTCGGCCATCTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggcg < 2:318772/149‑1 (MQ=255) ggggcggcTTTCCGCCGGCTTTTTTCCCCCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggcg < 2:775044/141‑1 (MQ=255) ggcgtcTGTCCGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGaa < 1:1070772/143‑1 (MQ=255) ggcggtTTTCTCCCATTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTc < 2:875240/150‑1 (MQ=255) ggcggtTGTCGCCCATTTTTTTTCCCCCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCt < 1:977023/149‑1 (MQ=255) ggcggcTGTCTGCCATTTTTTTTCCCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTcc < 2:919422/151‑1 (MQ=255) ggcggcTGTCTCCCTGTTTTTTTCCCGCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCt < 1:473264/149‑1 (MQ=255) cAGTTATTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTa < 1:498929/150‑1 (MQ=255) | TGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTA > NC_000913/357727‑358019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |