| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 52.9% | 7.5 / 47.4 | 34 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (14/2); new base A (0/18); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.62e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-06 | |||||||||||
GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGA > NC_000913/357742‑358038 | gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca < 2:659290/150‑1 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑c > 1:1100999/1‑151 (MQ=255) tAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑ccgccg > 2:31148/1‑151 (MQ=255) aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGt > 1:919355/1‑151 (MQ=255) cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTtctc > 1:725895/1‑151 (MQ=255) cATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTtctc > 2:783481/1‑151 (MQ=255) aTCAGGCAATGCCAGCCGCAGACCTGAGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGACGTtct > 1:355597/1‑149 (MQ=255) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑gg > 1:190301/1‑151 (MQ=255) ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑gggg > 2:814014/1‑151 (MQ=255) ccgtgtgCCGGCGTCTCCGGTGATTTTTTCCCCCAAACACCCGCCCTTTTACCCAACCCCATG‑GCAAAAAGTCCCTC‑C‑GCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAag < 2:1219728/146‑1 (MQ=255) ccTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGCGGC‑ggggg > 1:5610/1‑144 (MQ=255) tgtgTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑ggggggacagg > 1:693371/1‑142 (MQ=255) ctgCTACGGTATTTCTTTCCCGCCAACACCCCCGCTTTTACCCAACCCCTGGTG‑AAAAA‑AGCCTTCC‑GCGGGGGCTTTCTCCAGGCTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAAt < 2:731707/142‑1 (MQ=255) ctccggtgtTTCTTTCCCGCCAACACCCGCCCTTTTACCCACCGCCTTGTGAAAAAA‑TCCCTC‑C‑GGGGCGCTTGTCTCCCAGCTATTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAg < 2:836702/145‑1 (MQ=255) aCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAGaaaaaaaattaaaagc > 2:585065/1‑138 (MQ=25) attttCATCCCCCCCACCACCCGCCCTTTCACCCACCGCATTGTCAAAAAC‑GGCCTC‑C‑GGGGCGCCTGTCTCCCAGTTTTTTTCCCGCCACAAAAAAG‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGc < 1:247189/142‑1 (MQ=255) taaatcccccccAACACCCGCGCTTTTACCCAACGCCATGTGAAAAAA‑T‑CCTT‑CGGGGGGGGCTGTCTGCCAGTTTTTTTTCCGCCCAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCAt < 2:431267/139‑1 (MQ=255) cATTCCCCCCACCACCCGCGCATTTCTCCAACGCAATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTTTTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgtt < 1:1141385/150‑1 (MQ=255) caacCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGGCAGAGAAATAAATTTTAACCCCGCCTGTTTTGTgg > 2:502570/1‑144 (MQ=25) aTTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGCGGGGGCAAAGAAAAAAGCTTTAACACCG‑CCTGTTTTGTgggggagtct > 2:39833/1‑135 (MQ=25) caacccccATGTAAAAAAA‑TCCCTC‑C‑GGGGGGCCTGCCTCCCATTTTTTTTCCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc < 1:918972/140‑1 (MQ=255) ccATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGAGAAAAAAATTTTAAATCCCCATGTTGTGTGGGAATAGCTGCTGGGaagcaagagggaa > 2:491663/1‑139 (MQ=25) ccATGTAAAAAAA‑TCCTTC‑C‑GGGGCGGTTGTCTCCCAGCTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAg < 2:625501/151‑1 (MQ=255) attcccTC‑C‑GGGGCGGCGTTCGGCCGGTTTTTTTCCCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggc < 1:308755/147‑1 (MQ=255) acgCCTT‑C‑GCGGCGCGTGTCGGCCATCTTTTTTCCCGCCCCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggcg < 2:318772/149‑1 (MQ=255) ggggcggcTTTCCGCCGGCTTTTTTCCCCCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcggcg < 2:775044/141‑1 (MQ=255) ggcgtcTGTCCGCCAGTTTTTTTCCCGCCGAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGaa < 1:1070772/143‑1 (MQ=255) ggcggtTTTCTCCCATTTTTTTTCCCGCCGCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTc < 2:875240/150‑1 (MQ=255) ggcggtTGTCGCCCATTTTTTTTCCCCCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCt < 1:977023/149‑1 (MQ=255) ggcggcTGTCTGCCATTTTTTTTCCCGCCCAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTcc < 2:919422/151‑1 (MQ=255) ggcggcTGTCTCCCTGTTTTTTTCCCGCCGCAAAAAAC‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCt < 1:473264/149‑1 (MQ=255) cAGTTATTTTCCCGCCAAAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTa < 1:498929/150‑1 (MQ=255) cattttttttCCCCCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgcg < 1:783437/142‑1 (MQ=255) ttttcCCCCCACAAAAAAACCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg < 1:751598/149‑1 (MQ=255) cccgccaCAAAAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGtt < 1:253010/147‑1 (MQ=255) aacaaAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGa < 1:295923/151‑1 (MQ=255) | GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAA‑CCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAATGA > NC_000913/357742‑358038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |