Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,486	0	C	A	40.8%	99.9 / 50.6	72	intergenic (+291/‑165)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (29/13);  new base A (0/29);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 4.12e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAT  >  NC_000913/29336‑29635                                                                                                                                                             |                                                                                                                                                      tttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑cac                                                                                                                                                       >  1:121818/1‑151 (MQ=255)    ttgttgTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACaa                                                                                                                                                     >  1:733592/1‑150 (MQ=255)      gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAaat                                                                                                                                                  <  1:1080976/151‑1 (MQ=255)       ttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAaat                                                                                                                                                  >  1:1101159/1‑150 (MQ=255)         gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAaataa                                                                                                                                                <  1:566389/150‑1 (MQ=255)          ttttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAaataat                                                                                                                                               >  1:686192/1‑150 (MQ=255)           tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAataata                                                                                                                                              <  2:1267679/150‑1 (MQ=255)             tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAAtata                                                                                                                                            <  2:194793/150‑1 (MQ=255)              caTGTACAGTGTGACCGTTTAGTCCACTTTTTTCTGCTCGTTTTTATTTCATTCAATCTTGTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGTCTTTAAAACAAGCATGAGC‑CACAAgataat                                                                                                                                               >  1:1204323/2‑146 (MQ=255)              aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATaaa                                                                                                                                          <  2:339005/151‑1 (MQ=255)               aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATaaa                                                                                                                                          >  2:600307/1‑150 (MQ=255)                  tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATaaaaaa                                                                                                                                       <  1:39954/150‑1 (MQ=255)                   aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAAt                                                                                                                                      <  1:437955/150‑1 (MQ=255)                   aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATc                                                                                                                                     <  1:32045/151‑1 (MQ=255)                   aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATc                                                                                                                                     <  2:1142935/151‑1 (MQ=255)                   aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATc                                                                                                                                     <  2:1047748/151‑1 (MQ=255)                    aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATcc                                                                                                                                    <  1:389348/151‑1 (MQ=255)                    aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATc                                                                                                                                     >  1:1238031/1‑150 (MQ=255)                      ttttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCg                                                                                                                                  >  2:1009328/1‑151 (MQ=255)                                     ccttttttttCTGCTCTTTTTTATTTCATGAATTCTTCTTGCTGCGCAAGCTTTT‑TCCAA‑AA‑CGGT‑TT‑AGATGATTTTTTTGTCTCTTAATGCCTGAAAAACATGCATGACC‑CAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACtt                                                                                                                   <  2:452908/147‑1 (MQ=255)                                      cACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                  >  2:96297/1‑151 (MQ=255)                                         tttttccgtctctTTTTTTTTTCACCCATTCTTTTTCCTGCGAATGCTTTT‑CCAAA‑AA‑CAGT‑TT‑AGATAATTTTTTTGTCGATATGGTCCTTTAAAACATGCATGAGC‑AAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTa                                                                                                                <  1:1155432/141‑1 (MQ=255)                                           ttttcttttcTTTTTTATTGCATGCATTCTTTTTGCT‑CGCAGGCGTTTCGCCAG‑AG‑CGGG‑TT‑ATATTTTTTTTTTATCGATTACCCGCTG‑AAAACCTTTTTGGCCGCAGAAAAAAATAAAAAAAGTCCCGCCATTAAGTTGACTTTTAg                                                                                                               <  1:55618/149‑1 (MQ=255)                                           ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                             >  2:88485/1‑151 (MQ=255)                                            tttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcgc                                                                                                            >  1:703727/1‑151 (MQ=255)                                               cTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                          >  2:91555/1‑150 (MQ=255)                                                tGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                        >  2:556789/1‑151 (MQ=255)                                                      ttttttttTCATGAATTTTTTTTGCTGCGAAGGCGTTT‑CCAAA‑AA‑AAGG‑TA‑AAATTTTTTTTTTTTCGTTTATTCCTTAAAAAACATACTTGCGC‑AAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                    <  1:1281414/149‑1 (MQ=255)                                                       ttttttttCATGCATTTTTTTTGCTGCGAAAG‑TTTT‑TCCAA‑AA‑AAAG‑TTAAAATAATTTTTTTGCCGCTTAATGCCTAAAAAACATGCTTGACC‑CAAAAAATAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                  <  1:131180/150‑1 (MQ=255)                                                        tttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGTTTAATGCCTGTAAAACATCCATGAGC‑CACAAATTAATTTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                   >  2:35014/1‑148 (MQ=255)                                                          tATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                               >  2:369876/1‑150 (MQ=255)                                                           aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                             >  2:93338/1‑151 (MQ=255)                                                                 tGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                       >  1:724993/1‑151 (MQ=255)                                                                      tcttctTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                  >  2:154336/1‑151 (MQ=255)                                                                        ttctgGCTGCGCAGGC‑TTT‑TCCAA‑AA‑AAGGTTT‑AGATGTTTTTTTTGTCTTTTAATGCTTGAAAAACAGGAGGGAGC‑CAAAAAAAAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                     <  2:14223/146‑1 (MQ=255)                                                                             gctgcGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                            >  1:1063668/1‑150 (MQ=255)                                                                               ctgcgaacgcttt‑tCCAA‑AACCAGT‑TG‑AGATAATTTTTTGGTCGCTAAAGCCCTGAAAACCTTCCTTGCCC‑CAAAAAATAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                            <  1:485658/139‑1 (MQ=255)                                                                                gcgaaggttttt‑tCCAA‑AA‑CAGT‑TT‑AGATGATTTTTTTGTCCATAAACGCCTGAAAAACTTGCATGACC‑CAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                          <  2:914576/140‑1 (MQ=255)                                                                                    aaGTGTTT‑TCCAA‑AA‑CGGA‑AA‑AGATTATTTTTTTGGCCTTTAATGCCAGTAAAACTGGAATGAGC‑AAAAAAAAAATAAAAAAAAGCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcg                                                                       <  2:36132/148‑1 (MQ=255)                                                                                    aaGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATTTTTTTGTCTCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtc                                                                     <  2:1195942/150‑1 (MQ=255)                                                                                       gcttt‑tCCAAAAA‑CAGT‑TT‑AAATTTTTTTTTTTTCGATTAATCCCTAAAAAACTTGAATGAAC‑CAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGt                                                                  <  1:356567/149‑1 (MQ=255)                                                                                         ttt‑tCCAG‑AC‑AAGG‑TT‑TGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGACAGAAATTCGTCGGt                                                                  >  1:921124/1‑148 (MQ=255)                                                                                             tCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                              >  2:1014954/1‑149 (MQ=255)                                                                                                   aa‑CAGT‑TT‑AAATGTTCTTTTTGTCCTTTAACCCCTAAAAAACTTGATTGATC‑AACAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                        <  1:939558/150‑1 (MQ=255)                                                                                                     aacggttt‑AGATGATTTTTTTTTCGTTAAATCCCTAAAAAACATGCATGACC‑CAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCTAGAAATTCGTCGGTAAGCAGATTTg                                                       <  2:362034/145‑1 (MQ=255)                                                                                                            t‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                                >  2:726831/1‑151 (MQ=255)                                                                                                              aaattatCTTTTTGTCCTTTATTGCTTAAAAAACGTCCATGAGC‑CAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                  <  2:941423/143‑1 (MQ=255)                                                                                                                 ttttttttttGTCTTTTAATCCCTATAAAACAGGCATGACC‑CAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                               <  2:1064747/143‑1 (MQ=255)                                                                                                                 tgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                            >  2:1246987/1‑151 (MQ=255)                                                                                                                    tttttttCTCGTTTAATCCTTAAAAAACATGAAGGACC‑CAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                               <  2:932958/143‑1 (MQ=255)                                                                                                                      ttttttTCGATTAATGCCTAAAAAACATGCAGGAGC‑AAAAAAAAAAAATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                        <  1:385353/150‑1 (MQ=255)                                                                                                                       ttttGTCGTTTATTGCCTAAAAAACTTGCTGGACC‑CAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcat                                       <  1:825156/150‑1 (MQ=255)                                                                                                                         ttGTCCCTAAATGCCTAAAAAACGTGCATGACC‑CAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                     <  2:973869/150‑1 (MQ=255)                                                                                                                           gtcctttATTGCCAGAAAAACAGACAGGACC‑AAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                   <  1:1208463/145‑1 (MQ=255)                                                                                                                             cGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                >  2:1255628/1‑151 (MQ=255)                                                                                                                              gctcagtgccgaaaaaaTATTCATGAGC‑CAAAAAATAAAATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                <  1:490868/137‑1 (MQ=255)                                                                                                                              gCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                >  2:919027/1‑150 (MQ=255)                                                                                                                                 tAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                            >  1:1255138/1‑151 (MQ=255)                                                                                                                                  aaTGCCTAAAAAACATGCAGGAGC‑AAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                            <  2:812343/150‑1 (MQ=255)                                                                                                                                      cttaaaaaaCAGGCATGACC‑AAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTaa                          <  1:1030031/143‑1 (MQ=255)                                                                                                                                      cctaaaaaaCATGTAGGACC‑CAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                        <  1:496102/145‑1 (MQ=255)                                                                                                                                       cTGAAAAACATGCATGAGC‑CAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                         <  2:179381/148‑1 (MQ=255)                                                                                                                                        tgaaaaaCAGGCATGAGC‑AAAAAAAAAATATAAAAAGCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                             <  2:387444/140‑1 (MQ=255)                                                                                                                                          aaaaacggacatgAGC‑CAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAatat                       <  1:458090/138‑1 (MQ=255)                                                                                                                                           aaaaCATGAATGAGC‑CAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa                    <  2:593666/149‑1 (MQ=255)                                                                                                                                              aCATGCTGGACC‑CAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa               <  1:422254/151‑1 (MQ=255)                                                                                                                                              aCATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATa                >  1:431650/1‑150 (MQ=255)                                                                                                                                                  gcatgAGC‑CACAAAATAATATAAAAAATCCTGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg           >  1:999288/1‑151 (MQ=255)                                                                                                                                                    atgAGC‑CAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga          <  1:369862/150‑1 (MQ=255)                                                                                                                                                       aGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg       >  2:239590/1‑150 (MQ=255)                                                                                                                                                        cc‑cAAAAAATAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt        <  1:232188/147‑1 (MQ=255)                                                                                                                                                         c‑cACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat    >  2:964666/1‑151 (MQ=255)                                                                                                                                                         c‑cAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGaa     <  2:558173/150‑1 (MQ=255)                                                                                                                                                            acaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat  >  2:181741/1‑151 (MQ=255)                                                                                                                                                             |                                                                                                                                                      TTTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTT‑TCCAG‑AA‑CAGG‑TT‑AGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGC‑CACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAT  >  NC_000913/29336‑29635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑