Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,185,199	0	T	G	40.5%	106.5 / 26.7	75	T35P (ACC→CCC)	thiM	hydoxyethylthiazole kinase
Reads supporting (aligned to +/- strand):  ref base T (13/31);  new base G (30/0);  total (44/31)
Fisher's exact test for biased strand distribution p-value = 1.24e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTG  >  NC_000913/2185052‑2185349                                                                                                                                                      |                                                                                                                                                       caCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGgtt                                                                                                                                                      >  1:995616/1‑150 (MQ=255)     cATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGcacc                                                                                                                                                 >  1:630761/1‑151 (MQ=255)      aTCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAAGGTTTGaac                                                                                                                                                  >  2:949025/1‑150 (MQ=255)      aTCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGcacca                                                                                                                                                <  2:817529/151‑1 (MQ=255)      aTCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGcacc                                                                                                                                                 >  1:754561/1‑150 (MQ=255)        cGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGcaccac                                                                                                                                               <  2:351645/150‑1 (MQ=255)              aGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTT‑AAAGGttgccacacaccactt                                                                                                                                         >  1:786436/1‑137 (MQ=255)                 gcgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTTtaccaccgt                                                                                                                                            >  1:428301/1‑141 (MQ=255)                  cgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCAACAcatcaat                                                                                                                                         >  1:372916/1‑144 (MQ=255)                   gTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCa                                                                                                                                    <  1:330813/150‑1 (MQ=255)                   gTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCCCCCCATCATTGGTCAt                                                                                                                                   >  1:1052582/1‑151 (MQ=255)                           gTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCCCAACATTGGTCATGCAGTg                                                                                                                             >  1:1201723/1‑149 (MQ=255)                             cAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACCCCATTTGTCATGCAGTGcac                                                                                                                          >  2:726590/1‑150 (MQ=255)                              aGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACACAATTTGGCATGCAGt                                                                                                                              >  2:609117/1‑145 (MQ=255)                                 gtgCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAagag                                                                                                                     <  1:736507/151‑1 (MQ=255)                                 gtgCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAagag                                                                                                                     <  1:429538/151‑1 (MQ=255)                                 gtgCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGTCATGCCGTGCACaa                                                                                                                        >  1:5110/1‑148 (MQ=255)                                       aCGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑C‑AAAGGTTTGCGCCCCATCATTGGTCCTGCAGTGCACAAGAgggg                                                                                                                  >  1:1215193/1‑148 (MQ=255)                                         gTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGt                                                                                                             <  2:75275/151‑1 (MQ=255)                                           tAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGtt                                                                                                            <  2:998554/150‑1 (MQ=255)                                            aaTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAAGGTTTG‑AACACATCATTTGGCAAGCAATGGACAAAAGGGGGATggt                                                                                                            >  2:690384/1‑147 (MQ=255)                                             aTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGt                                                                                                         <  2:100575/151‑1 (MQ=255)                                              tCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTTGACCACCTCATTGGGGCTGCAGGGGACCAGAGGGGGATGTTGGTg                                                                                                        >  2:949579/1‑151 (MQ=255)                                              tCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGGATGTTgggg                                                                                                        >  2:290994/1‑149 (MQ=255)                                               caacaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑CAAGGTTTGCACCCCATCATTGGTCATGCAGAGCACCACAGGGGGATGTTgggg                                                                                                        >  2:762443/1‑148 (MQ=255)                                               caacaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTTCACCACACCATTTGGCACGCAGCGCACAAGAGGGGAAAGTTGGTg                                                                                                        >  2:782446/1‑150 (MQ=255)                                                aacaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGaa                                                                                                      <  2:444420/151‑1 (MQ=255)                                                aacaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGaa                                                                                                      <  1:1094901/151‑1 (MQ=255)                                                 acaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTTGTCATGCAGTGCACAAGAGGGGGATGTTgggg                                                                                                        >  2:112424/1‑146 (MQ=255)                                                    aGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGTCATGCAGGGCACAAGAGGGGAATGTTGGGGgaaa                                                                                                    >  1:1087978/1‑149 (MQ=255)                                                       cACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGgt                                                                                                <  1:943762/150‑1 (MQ=255)                                                           ggCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTaa                                                                                            <  1:755680/150‑1 (MQ=255)                                                           ggCGATAGCCGCAAACTGACTGGCCTCTGCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTaa                                                                                            <  1:675685/150‑1 (MQ=255)                                                               aTAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGTCATGCCGGGCACAAGAGGGGGATGTTGGGGAAAAAGGGGTAAcg                                                                                          >  1:431091/1‑148 (MQ=255)                                                                tAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑TAAGGTTTGCCCCCCATCACTCGTCATTTTGCGCACCAGAGGGGGGTGTTGGTGAAAAACGTGGAAAGCGTg                                                                                      >  2:582795/1‑151 (MQ=255)                                                                     gCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGTCATTCAGTGCACAAGAGGGGGATGTTGGTGGAAAAGGGGTAACGCGGgcg                                                                                    >  1:581734/1‑148 (MQ=255)                                                                     gCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGGCATGCAGTGCACAAGAGGGGGAAGTTGGTGGAAAAGGGGTAAAGCGTgcg                                                                                    >  1:736526/1‑148 (MQ=255)                                                                            gactgGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTgcgc                                                                          <  2:374064/151‑1 (MQ=255)                                                                              ctgGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACTACATCATTTGTCATGCCGTGCACAAGAGGGGGATGTTGGGGGAAAAAGGGTAACGCGTGCGCAGATTgcg                                                                           >  2:1267898/1‑148 (MQ=255)                                                                                    tctTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAAcc                                                                    <  1:1048674/149‑1 (MQ=255)                                                                                     ctTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑A‑AAAGGGTTGCACCACATCATTGGTCATTCAGTGCACAAGAGGGGCATGTTGGTGAAAAAGGGGTAACGCGGGCGCAGATTGCGCTGAAacc                                                                   >  1:750469/1‑149 (MQ=255)                                                                                       tCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCACGGTATTGGCGG‑A‑AAAAGGTTTCACCACACCCTTGGGCGTCCAGTGGACCAGAGGGGGAAGTTGGGGGAAAAGGTGTAAAGCGGGGGCCGATTTGGCTGAAcc                                                                    >  2:940280/1‑146 (MQ=255)                                                                                        cGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCcagca                                                               <  2:907289/150‑1 (MQ=255)                                                                                         ggTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAgg                                                             <  2:1089076/151‑1 (MQ=255)                                                                                         ggTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGGCATGCAGGGCACAAGAGGGGGATGTTGGGGGAAAAGGGGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAgg                                                             >  1:428895/1‑151 (MQ=255)                                                                                           tttCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGATTTCACCCCATCATTCGTTCGGCAGGGGCCAAAAGGGGATTGGTCGTGGAGAAGGTGTAACGCGTGCCTAGATTGGCCTGAACgcagcaag                                                             >  2:47133/1‑147 (MQ=255)                                                                                           tttCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAAGGTTGCACCACATCATTGGTCATGCCGGGCACAAGAGGGGGAAGGTGGGGGAAAAGGGGTAACGCGGGCGCAGATTGCGGTGAAACCAACAgg                                                             >  1:159883/1‑149 (MQ=255)                                                                                            ttCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTc                                                           <  2:56925/150‑1 (MQ=255)                                                                                             tCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTc                                                           <  2:873551/149‑1 (MQ=255)                                                                                             tCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGGCATGCAGGGCACAAGAGGGGAATGTTGGGGAAAAAGGTGGAAAGCGTGCGCAGATTGCGCTGAAACCAGCAgg                                                             >  2:252252/1‑147 (MQ=255)                                                                                              cGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGGATGTTGGGGAAAAAGGTGTAACGCGGGCGCAGATTGGGCTGAACCCAGCAGGTCg                                                          >  1:443930/1‑149 (MQ=255)                                                                                              cGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑C‑TAAGGGTTGCCCCACATCCTTTGGCCTGCAGGGCACCAGAGGGGGATGTTGGGGAAAAAGGGGGAAAGCGTGGGGAGATTGCCGTGAAGccaccaaggtgt                                                         >  2:635749/1‑144 (MQ=255)                                                                                                aTAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGGATGTTGGTGAAAAAGGGGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGGCGACtt                                                      >  1:1122892/1‑151 (MQ=255)                                                                                                 tAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCACATCATTTGTCATGCAGTGCACAAGAGGGGGATGTTGGTGGAAAAGGGGTAACGCGTGCGCCGATTGCGCTGAACCCAGCAGGTCGACtt                                                      >  1:279432/1‑150 (MQ=255)                                                                                                  aaCCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTg                                                     <  1:451584/150‑1 (MQ=255)                                                                                                   aCCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAAGGTTGCACCCCAACATTGGGTATGGCGGGGACCAAAGGGGGAGGGTGGGGGAAAAAGTGTAACGCGGGGGCAGGTTGGGCTGAACCCAGCAgggctggt                                                       >  2:1050733/1‑141 (MQ=255)                                                                                                      aTCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAAGTTTGCACCACATCATTTGTCATGCAGTGCACAAGAGGGGGATGTTGGTGAAAAAAGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCCGGTCGACTTGCAt                                                  >  1:1156933/1‑149 (MQ=255)                                                                                                        cGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGt                                               <  1:820166/150‑1 (MQ=255)                                                                                                          cTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGttt                                             <  1:1024679/150‑1 (MQ=255)                                                                                                              cGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTTGTCATGCCGGGCACAAGAGGGGGATGTTGGTGAAAAAGGTGTAAAGCGTGGGGAGATTGAGCTGAACCCCGCAGGTCGACTTGCATAGTTTTCTcc                                        >  2:1148904/1‑151 (MQ=255)                                                                                                               gATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCt                                       <  2:778056/151‑1 (MQ=255)                                                                                                                aTGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGGTTGCACCCCAACCCTGGTCCTGTAGGGCACAAGAGGGGGATGTTGGTGAAAAAAGGGTAACGCCTGCGCAGATTGCGCTGGAACCAGCCGGGCGACTTGCATAGTTTGCTcc                                        >  2:1195336/1‑149 (MQ=255)                                                                                                                         gcgcCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACg                              <  2:515068/150‑1 (MQ=255)                                                                                                                         gcgcCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGt                             <  1:417900/151‑1 (MQ=255)                                                                                                                         gcgcCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGt                             <  2:152353/151‑1 (MQ=255)                                                                                                                          cgcCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTg                            <  2:553998/151‑1 (MQ=255)                                                                                                                             cAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTgaag                         <  2:995547/151‑1 (MQ=255)                                                                                                                              aGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTgaaga                        <  1:848733/151‑1 (MQ=255)                                                                                                                               gCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGGCATAACGTgaagaa                       <  2:344442/151‑1 (MQ=255)                                                                                                                               gCAAGGTATTGGCGG‑T‑AAAGGGTTTCACCACATCATTGGTCATGCAGGGCACAAGGGGGGGAGGTTGGTGGAAAAGGGGTAACGCGGGCGCAGATTGCGCTGAACCCCGCAGGTCGACTTTCATTATTTGCTCCTGCCATAAAGGgaa                          >  2:1038446/1‑148 (MQ=255)                                                                                                                                 aaGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGGGAAAAAGGGGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTgaagaa                       >  1:355422/1‑149 (MQ=255)                                                                                                                                        tGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGAcc              >  2:1063049/1‑151 (MQ=255)                                                                                                                                        tGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGAc               <  2:574725/150‑1 (MQ=255)                                                                                                                                           cGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCtgg           >  2:993682/1‑151 (MQ=255)                                                                                                                                               t‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCtggtgg        >  2:683368/1‑151 (MQ=255)                                                                                                                                                  aaGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTc      >  1:899518/1‑151 (MQ=255)                                                                                                                                                  aaGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTc      >  2:1019956/1‑151 (MQ=255)                                                                                                                                                      tttGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTg  >  1:29870/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                       CACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑T‑AAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTG  >  NC_000913/2185052‑2185349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑